Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q56YN3 (NADK1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD(H) kinase 1

Short name=AtNADK-1
EC=2.7.1.23
EC=2.7.1.86
Gene names
Name:NADK1
Ordered Locus Names:At3g21070
ORF Names:MSA6.11
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length524 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Phosphorylates both NAD+ and NADH, with a twofold preference for NADH. Source of the cellular reductant NADPH which is an important antioxidant factor. Ref.1

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

ATP + NADH = ADP + NADPH. HAMAP-Rule MF_00361

Tissue specificity

Ubiquitous. Ref.1 Ref.6

Developmental stage

Expressed during development from young seedlings to flowering plants. Ref.6

Induction

By gamma radiation, hydrogen peroxide and infection by P.syringae. Ref.1

Sequence similarities

Belongs to the NAD kinase family.

Biophysicochemical properties

Kinetic parameters:

Measured at pH 7.9 and 25 degrees Celsius for all experiments.

KM=520 µM for NAD Ref.6

KM=730 µM for ATP

Vmax=11.1 µmol/h/mg enzyme with ATP as substrate

pH dependence:

Optimum pH is 7.9 at 25 degrees Celsius.

Sequence caution

The sequence AAM13987.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAB01450.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q56YN3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 524524NAD(H) kinase 1 HAMAP-Rule MF_00361
PRO_0000233702

Experimental info

Sequence conflict1781M → I in AAM13987. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 2, 2006. Version 2.
Checksum: 529A4D659C1F9298

FASTA52458,245
        10         20         30         40         50         60 
MSSTYKLNHT DSFANGDAKS LLPNPENGFT HLTSLAQSEK AVQELLLQQT PMQATDDHLV 

        70         80         90        100        110        120 
EFSEALRTVA KALRGAAEGK ALAQAEAAEW KRRYELERSK NVELQHKELS NGVCADESNG 

       130        140        150        160        170        180 
QRMEHLAKSP RLYAQEISSN GMETICSHEV LQDGGFNSFN NKLKRKASFK LSWGCKGMAN 

       190        200        210        220        230        240 
DQHKKEIVSF ERGNISTAER SSKQISLTWE SDPQTVLIIT KPNSTSVRVL SVDMVRWLRT 

       250        260        270        280        290        300 
QKGLNIYVEP RVKEELLSES SSFNFVQTWE DDKEISLLHT KVDLLITLGG DGTVLWAASM 

       310        320        330        340        350        360 
FKGPVPPIVP FSMGSLGFMT PFHSEQYRDC LEAILKGPIS ITLRHRLQCH IIRDKATHEY 

       370        380        390        400        410        420 
EPEETMLVLN EVTIDRGISS YLTNLECYCD NSFVTCVQGD GLILSTTSGS TAYSLAAGGS 

       430        440        450        460        470        480 
MVHPQVPGIL FTPICPHSLS FRPLILPEHV TVRVQVPFNS RSSAWVSFDG KDRKQLEAGD 

       490        500        510        520 
ALVCSMAPWP VSTACQVEST NDFLRSIHDG LHWNLRKTQS ADGP 

« Hide

References

« Hide 'large scale' references
[1]"Stress induces the expression of AtNADK-1, a gene encoding a NAD(H) kinase in Arabidopsis thaliana."
Berrin J.-G., Pierrugues O., Brutesco C., Alonso B., Montillet J.-L., Roby D., Kazmaier M.
Mol. Genet. Genomics 273:10-19(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, TISSUE SPECIFICITY.
[2]"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Cloning and characterization of two NAD kinases from Arabidopsis. Identification of a calmodulin binding isoform."
Turner W.L., Waller J.C., Vanderbeld B., Snedden W.A.
Plant Physiol. 135:1243-1255(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY383545 mRNA. Translation: AAR32133.1.
AP000604 Genomic DNA. Translation: BAB01450.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE76456.1.
AY090937 mRNA. Translation: AAM13987.1. Different initiation.
AK176132 mRNA. Translation: BAD43895.1.
AK221288 mRNA. Translation: BAD94003.1.
RefSeqNP_974347.1. NM_202618.2. [Q56YN3-1]
UniGeneAt.5579.
At.72877.

3D structure databases

ProteinModelPortalQ56YN3.
SMRQ56YN3. Positions 207-512.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ56YN3.
PRIDEQ56YN3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G21070.2; AT3G21070.2; AT3G21070. [Q56YN3-1]
GeneID821659.
KEGGath:AT3G21070.

Organism-specific databases

TAIRAT3G21070.

Phylogenomic databases

eggNOGCOG0061.
HOGENOMHOG000029806.
InParanoidQ56YN3.
KOK00858.
PhylomeDBQ56YN3.

Enzyme and pathway databases

BioCycARA:GQT-1798-MONOMER.
BRENDA2.7.1.86. 399.

Gene expression databases

GenevestigatorQ56YN3.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNADK1_ARATH
AccessionPrimary (citable) accession number: Q56YN3
Secondary accession number(s): Q6TXT3, Q8RX27, Q9LJC5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 2, 2006
Last modified: June 11, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names