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Q56YN3

- NADK1_ARATH

UniProt

Q56YN3 - NADK1_ARATH

Protein

NAD(H) kinase 1

Gene

NADK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 2 (02 May 2006)
      Previous versions | rss
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    Functioni

    Phosphorylates both NAD+ and NADH, with a twofold preference for NADH. Source of the cellular reductant NADPH which is an important antioxidant factor.1 Publication

    Catalytic activityi

    ATP + NAD+ = ADP + NADP+.
    ATP + NADH = ADP + NADPH.

    Kineticsi

    Measured at pH 7.9 and 25 degrees Celsius for all experiments.

    1. KM=520 µM for NAD1 Publication
    2. KM=730 µM for ATP1 Publication

    Vmax=11.1 µmol/h/mg enzyme with ATP as substrate1 Publication

    pH dependencei

    Optimum pH is 7.9 at 25 degrees Celsius.1 Publication

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. NAD+ kinase activity Source: UniProtKB-EC
    3. NADH kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. NAD metabolic process Source: InterPro
    2. NADP biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, NAD, NADP, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:GQT-1798-MONOMER.
    BRENDAi2.7.1.86. 399.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NAD(H) kinase 1 (EC:2.7.1.23, EC:2.7.1.86)
    Short name:
    AtNADK-1
    Gene namesi
    Name:NADK1
    Ordered Locus Names:At3g21070
    ORF Names:MSA6.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G21070.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 524524NAD(H) kinase 1PRO_0000233702Add
    BLAST

    Proteomic databases

    PaxDbiQ56YN3.
    PRIDEiQ56YN3.

    Expressioni

    Tissue specificityi

    Ubiquitous.2 Publications

    Developmental stagei

    Expressed during development from young seedlings to flowering plants.1 Publication

    Inductioni

    By gamma radiation, hydrogen peroxide and infection by P.syringae.1 Publication

    Gene expression databases

    GenevestigatoriQ56YN3.

    Structurei

    3D structure databases

    ProteinModelPortaliQ56YN3.
    SMRiQ56YN3. Positions 207-512.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NAD kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0061.
    HOGENOMiHOG000029806.
    InParanoidiQ56YN3.
    KOiK00858.
    PhylomeDBiQ56YN3.

    Family and domain databases

    Gene3Di2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPiMF_00361. NAD_kinase.
    InterProiIPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR002504. PolyP/ATP_NADK.
    [Graphical view]
    PANTHERiPTHR20275. PTHR20275. 1 hit.
    PfamiPF01513. NAD_kinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q56YN3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSSTYKLNHT DSFANGDAKS LLPNPENGFT HLTSLAQSEK AVQELLLQQT    50
    PMQATDDHLV EFSEALRTVA KALRGAAEGK ALAQAEAAEW KRRYELERSK 100
    NVELQHKELS NGVCADESNG QRMEHLAKSP RLYAQEISSN GMETICSHEV 150
    LQDGGFNSFN NKLKRKASFK LSWGCKGMAN DQHKKEIVSF ERGNISTAER 200
    SSKQISLTWE SDPQTVLIIT KPNSTSVRVL SVDMVRWLRT QKGLNIYVEP 250
    RVKEELLSES SSFNFVQTWE DDKEISLLHT KVDLLITLGG DGTVLWAASM 300
    FKGPVPPIVP FSMGSLGFMT PFHSEQYRDC LEAILKGPIS ITLRHRLQCH 350
    IIRDKATHEY EPEETMLVLN EVTIDRGISS YLTNLECYCD NSFVTCVQGD 400
    GLILSTTSGS TAYSLAAGGS MVHPQVPGIL FTPICPHSLS FRPLILPEHV 450
    TVRVQVPFNS RSSAWVSFDG KDRKQLEAGD ALVCSMAPWP VSTACQVEST 500
    NDFLRSIHDG LHWNLRKTQS ADGP 524
    Length:524
    Mass (Da):58,245
    Last modified:May 2, 2006 - v2
    Checksum:i529A4D659C1F9298
    GO

    Sequence cautioni

    The sequence AAM13987.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAB01450.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti178 – 1781M → I in AAM13987. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY383545 mRNA. Translation: AAR32133.1.
    AP000604 Genomic DNA. Translation: BAB01450.1. Sequence problems.
    CP002686 Genomic DNA. Translation: AEE76456.1.
    AY090937 mRNA. Translation: AAM13987.1. Different initiation.
    AK176132 mRNA. Translation: BAD43895.1.
    AK221288 mRNA. Translation: BAD94003.1.
    RefSeqiNP_974347.1. NM_202618.2. [Q56YN3-1]
    UniGeneiAt.5579.
    At.72877.

    Genome annotation databases

    EnsemblPlantsiAT3G21070.2; AT3G21070.2; AT3G21070. [Q56YN3-1]
    GeneIDi821659.
    KEGGiath:AT3G21070.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY383545 mRNA. Translation: AAR32133.1 .
    AP000604 Genomic DNA. Translation: BAB01450.1 . Sequence problems.
    CP002686 Genomic DNA. Translation: AEE76456.1 .
    AY090937 mRNA. Translation: AAM13987.1 . Different initiation.
    AK176132 mRNA. Translation: BAD43895.1 .
    AK221288 mRNA. Translation: BAD94003.1 .
    RefSeqi NP_974347.1. NM_202618.2. [Q56YN3-1 ]
    UniGenei At.5579.
    At.72877.

    3D structure databases

    ProteinModelPortali Q56YN3.
    SMRi Q56YN3. Positions 207-512.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q56YN3.
    PRIDEi Q56YN3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G21070.2 ; AT3G21070.2 ; AT3G21070 . [Q56YN3-1 ]
    GeneIDi 821659.
    KEGGi ath:AT3G21070.

    Organism-specific databases

    TAIRi AT3G21070.

    Phylogenomic databases

    eggNOGi COG0061.
    HOGENOMi HOG000029806.
    InParanoidi Q56YN3.
    KOi K00858.
    PhylomeDBi Q56YN3.

    Enzyme and pathway databases

    BioCyci ARA:GQT-1798-MONOMER.
    BRENDAi 2.7.1.86. 399.

    Gene expression databases

    Genevestigatori Q56YN3.

    Family and domain databases

    Gene3Di 2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPi MF_00361. NAD_kinase.
    InterProi IPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR002504. PolyP/ATP_NADK.
    [Graphical view ]
    PANTHERi PTHR20275. PTHR20275. 1 hit.
    Pfami PF01513. NAD_kinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Stress induces the expression of AtNADK-1, a gene encoding a NAD(H) kinase in Arabidopsis thaliana."
      Berrin J.-G., Pierrugues O., Brutesco C., Alonso B., Montillet J.-L., Roby D., Kazmaier M.
      Mol. Genet. Genomics 273:10-19(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, TISSUE SPECIFICITY.
    2. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
      Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
      DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Cloning and characterization of two NAD kinases from Arabidopsis. Identification of a calmodulin binding isoform."
      Turner W.L., Waller J.C., Vanderbeld B., Snedden W.A.
      Plant Physiol. 135:1243-1255(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiNADK1_ARATH
    AccessioniPrimary (citable) accession number: Q56YN3
    Secondary accession number(s): Q6TXT3, Q8RX27, Q9LJC5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 2, 2006
    Last sequence update: May 2, 2006
    Last modified: October 1, 2014
    This is version 79 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3