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Q56YN3

- NADK1_ARATH

UniProt

Q56YN3 - NADK1_ARATH

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Protein
NAD(H) kinase 1
Gene
NADK1, At3g21070, MSA6.11
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Phosphorylates both NAD+ and NADH, with a twofold preference for NADH. Source of the cellular reductant NADPH which is an important antioxidant factor.1 Publication

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation
ATP + NADH = ADP + NADPH.UniRule annotation

Kineticsi

Measured at pH 7.9 and 25 degrees Celsius for all experiments.

  1. KM=520 µM for NAD1 Publication
  2. KM=730 µM for ATP

Vmax=11.1 µmol/h/mg enzyme with ATP as substrate

pH dependencei

Optimum pH is 7.9 at 25 degrees Celsius.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. NAD+ kinase activity Source: UniProtKB-EC
  3. NADH kinase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:GQT-1798-MONOMER.
BRENDAi2.7.1.86. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD(H) kinase 1 (EC:2.7.1.23, EC:2.7.1.86)
Short name:
AtNADK-1
Gene namesi
Name:NADK1
Ordered Locus Names:At3g21070
ORF Names:MSA6.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G21070.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 524524NAD(H) kinase 1UniRule annotation
PRO_0000233702Add
BLAST

Proteomic databases

PaxDbiQ56YN3.
PRIDEiQ56YN3.

Expressioni

Tissue specificityi

Ubiquitous.2 Publications

Developmental stagei

Expressed during development from young seedlings to flowering plants.1 Publication

Inductioni

By gamma radiation, hydrogen peroxide and infection by P.syringae.1 Publication

Gene expression databases

GenevestigatoriQ56YN3.

Structurei

3D structure databases

ProteinModelPortaliQ56YN3.
SMRiQ56YN3. Positions 207-512.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000029806.
InParanoidiQ56YN3.
KOiK00858.
PhylomeDBiQ56YN3.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q56YN3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSSTYKLNHT DSFANGDAKS LLPNPENGFT HLTSLAQSEK AVQELLLQQT    50
PMQATDDHLV EFSEALRTVA KALRGAAEGK ALAQAEAAEW KRRYELERSK 100
NVELQHKELS NGVCADESNG QRMEHLAKSP RLYAQEISSN GMETICSHEV 150
LQDGGFNSFN NKLKRKASFK LSWGCKGMAN DQHKKEIVSF ERGNISTAER 200
SSKQISLTWE SDPQTVLIIT KPNSTSVRVL SVDMVRWLRT QKGLNIYVEP 250
RVKEELLSES SSFNFVQTWE DDKEISLLHT KVDLLITLGG DGTVLWAASM 300
FKGPVPPIVP FSMGSLGFMT PFHSEQYRDC LEAILKGPIS ITLRHRLQCH 350
IIRDKATHEY EPEETMLVLN EVTIDRGISS YLTNLECYCD NSFVTCVQGD 400
GLILSTTSGS TAYSLAAGGS MVHPQVPGIL FTPICPHSLS FRPLILPEHV 450
TVRVQVPFNS RSSAWVSFDG KDRKQLEAGD ALVCSMAPWP VSTACQVEST 500
NDFLRSIHDG LHWNLRKTQS ADGP 524
Length:524
Mass (Da):58,245
Last modified:May 2, 2006 - v2
Checksum:i529A4D659C1F9298
GO

Sequence cautioni

The sequence AAM13987.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAB01450.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti178 – 1781M → I in AAM13987. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY383545 mRNA. Translation: AAR32133.1.
AP000604 Genomic DNA. Translation: BAB01450.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE76456.1.
AY090937 mRNA. Translation: AAM13987.1. Different initiation.
AK176132 mRNA. Translation: BAD43895.1.
AK221288 mRNA. Translation: BAD94003.1.
RefSeqiNP_974347.1. NM_202618.2. [Q56YN3-1]
UniGeneiAt.5579.
At.72877.

Genome annotation databases

EnsemblPlantsiAT3G21070.2; AT3G21070.2; AT3G21070. [Q56YN3-1]
GeneIDi821659.
KEGGiath:AT3G21070.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY383545 mRNA. Translation: AAR32133.1 .
AP000604 Genomic DNA. Translation: BAB01450.1 . Sequence problems.
CP002686 Genomic DNA. Translation: AEE76456.1 .
AY090937 mRNA. Translation: AAM13987.1 . Different initiation.
AK176132 mRNA. Translation: BAD43895.1 .
AK221288 mRNA. Translation: BAD94003.1 .
RefSeqi NP_974347.1. NM_202618.2. [Q56YN3-1 ]
UniGenei At.5579.
At.72877.

3D structure databases

ProteinModelPortali Q56YN3.
SMRi Q56YN3. Positions 207-512.
ModBasei Search...

Proteomic databases

PaxDbi Q56YN3.
PRIDEi Q56YN3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G21070.2 ; AT3G21070.2 ; AT3G21070 . [Q56YN3-1 ]
GeneIDi 821659.
KEGGi ath:AT3G21070.

Organism-specific databases

TAIRi AT3G21070.

Phylogenomic databases

eggNOGi COG0061.
HOGENOMi HOG000029806.
InParanoidi Q56YN3.
KOi K00858.
PhylomeDBi Q56YN3.

Enzyme and pathway databases

BioCyci ARA:GQT-1798-MONOMER.
BRENDAi 2.7.1.86. 399.

Gene expression databases

Genevestigatori Q56YN3.

Family and domain databases

Gene3Di 2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPi MF_00361. NAD_kinase.
InterProi IPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view ]
PANTHERi PTHR20275. PTHR20275. 1 hit.
Pfami PF01513. NAD_kinase. 1 hit.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Stress induces the expression of AtNADK-1, a gene encoding a NAD(H) kinase in Arabidopsis thaliana."
    Berrin J.-G., Pierrugues O., Brutesco C., Alonso B., Montillet J.-L., Roby D., Kazmaier M.
    Mol. Genet. Genomics 273:10-19(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, TISSUE SPECIFICITY.
  2. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Cloning and characterization of two NAD kinases from Arabidopsis. Identification of a calmodulin binding isoform."
    Turner W.L., Waller J.C., Vanderbeld B., Snedden W.A.
    Plant Physiol. 135:1243-1255(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiNADK1_ARATH
AccessioniPrimary (citable) accession number: Q56YN3
Secondary accession number(s): Q6TXT3, Q8RX27, Q9LJC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 2, 2006
Last modified: June 11, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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