Reviewed,
UniProtKB/Swiss-Prot Q56YN3 (NADK1_ARATH)
Last modified
October 13, 2009.
Version 38.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: NAD(H) kinase 1 Short name=AtNADK-1 EC=2.7.1.23 EC=2.7.1.86 | ||||||
| Gene names |
| ||||||
| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 524 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Phosphorylates both NAD+ and NADH, with a twofold preference for NADH. Source of the cellular reductant NADPH which is an important antioxidant factor. Ref.1 |
| Catalytic activity | ATP + NAD+ = ADP + NADP+. ATP + NADH = ADP + NADPH. |
| Tissue specificity | |
| Developmental stage | Expressed during development from young seedlings to flowering plants. Ref.5 |
| Induction | By gamma radiation, hydrogen peroxide and infection by P.syringae. Ref.1 |
| Sequence similarities | Belongs to the NAD kinase family. |
| Biophysicochemical properties | Kinetic parameters: Measured at pH 7.9 and 25 degrees Celsius for all experiments. KM=520 µM for NAD KM=730 µM for ATP Vmax=11.1 µmol/h/mg enzyme with ATP as substrate pH dependence: Optimum pH is 7.9 at 25 degrees Celsius. |
| Sequence caution | The sequence BAB01450.1 differs from that shown. Reason: Erroneous gene model prediction. |
Ontologies
| Keywords | |
|---|---|
| Coding sequence diversity | Alternative splicing |
| Ligand | ATP-binding NAD NADP Nucleotide-binding |
| Molecular function | Kinase Transferase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | NADP biosynthetic process Ref.1 Traceable author statement. Source: TAIR |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Ref.1Inferred from direct assay. Source: TAIR NADH kinase activity Ref.1Inferred from direct assay. Source: TAIR |
| Complete GO annotation... | |
Alternative products
| This entry describes 1 isoform produced by alternative splicing. [Select] Note: A number of isoforms are produced. According to EST sequences. | ||||||
| Isoform 1 (identifier: Q56YN3-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. |
Sequence annotation (Features)
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Stress induces the expression of AtNADK-1, a gene encoding a NAD(H) kinase in Arabidopsis thaliana." Berrin J.-G., Pierrugues O., Brutesco C., Alonso B., Montillet J.-L., Roby D., Kazmaier M. Mol. Genet. Genomics 273:10-19(2005) [PubMed: 15711971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, TISSUE SPECIFICITY. |
| [2] | "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [4] | "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. Shinozaki K.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [5] | "Cloning and characterization of two NAD kinases from Arabidopsis. Identification of a calmodulin binding isoform." Turner W.L., Waller J.C., Vanderbeld B., Snedden W.A. Plant Physiol. 135:1243-1255(2004) [PubMed: 15247403] [Abstract] Cited for: BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AY383545 mRNA. Translation: AAR32133.1. AP000604 Genomic DNA. Translation: BAB01450.1. Sequence problems. AY090937 mRNA. Translation: AAM13987.1. Different initiation. AK176132 mRNA. Translation: BAD43895.1. AK221288 mRNA. Translation: BAD94003.1. | |
| IPI | IPI00531291. |
| RefSeq | NP_974347.1. |
| UniGene | At.5579 At.72877 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q56YN3. |
Proteomic databases | |
| PRIDE | Q56YN3. |
Genome annotation databases | |
| GeneID | 821659. |
| GenomeReviews | Gene locus AT3G21070 in contig BA000014_GR. |
| NMPDR | fig|3702.1.peg.14338. |
Organism-specific databases | |
| TAIR | At3g21070. |
Enzyme and pathway databases | |
| BRENDA | 2.7.1.23. 302. 2.7.1.86. 302. |
Gene expression databases | |
| Genevestigator | Q56YN3. |
Family and domain databases | |
| InterPro | IPR017437. ATP-NAD_kinase_PpnK-typ_all-b. IPR002504. ATP_NADK. [Graphical view] |
| Gene3D | G3DSA:2.60.200.30. ATP-NAD_kinase_PpnK-typ. 1 hit. |
| PANTHER | PTHR20275. ATP_NADK. 1 hit. |
| Pfam | PF01513. NAD_kinase. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | NADK1_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q56YN3 Secondary accession number(s): Q6TXT3, Q8RX27, Q9LJC5 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with


