Q56YN3 (NADK1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NAD(H) kinase 1
Short name=AtNADK-1
EC=2.7.1.23
EC=2.7.1.86

Gene names

Name:	NADK1
Ordered Locus Names:	At3g21070
ORF Names:	MSA6.11

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phosphorylates both NAD+ and NADH, with a twofold preference for NADH. Source of the cellular reductant NADPH which is an important antioxidant factor. Ref.1
Catalytic activity	ATP + NAD+ = ADP + NADP+. ATP + NADH = ADP + NADPH.
Tissue specificity	Ubiquitous. Ref.1 Ref.6
Developmental stage	Expressed during development from young seedlings to flowering plants. Ref.6
Induction	By gamma radiation, hydrogen peroxide and infection by P.syringae. Ref.1
Sequence similarities	Belongs to the NAD kinase family.
Biophysicochemical properties	Kinetic parameters: Measured at pH 7.9 and 25 degrees Celsius for all experiments. KM=520 µM for NAD Ref.6 KM=730 µM for ATP Vmax=11.1 µmol/h/mg enzyme with ATP as substrate pH dependence: Optimum pH is 7.9 at 25 degrees Celsius.
Sequence caution	The sequence AAM13987.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB01450.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding NAD NADP Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	NAD metabolic process Inferred from electronic annotation. Source: InterPro NADP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Inferred from electronic annotation. Source: EC NADH kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q56YN3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 524	524	NAD(H) kinase 1		PRO_0000233702
Experimental info
	Sequence conflict	178	1	M → I in AAM13987. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 2, 2006. Version 2. Checksum: 529A4D659C1F9298	FASTA	524	58,245
	10 20 30 40 50 60 MSSTYKLNHT DSFANGDAKS LLPNPENGFT HLTSLAQSEK AVQELLLQQT PMQATDDHLV 70 80 90 100 110 120 EFSEALRTVA KALRGAAEGK ALAQAEAAEW KRRYELERSK NVELQHKELS NGVCADESNG 130 140 150 160 170 180 QRMEHLAKSP RLYAQEISSN GMETICSHEV LQDGGFNSFN NKLKRKASFK LSWGCKGMAN 190 200 210 220 230 240 DQHKKEIVSF ERGNISTAER SSKQISLTWE SDPQTVLIIT KPNSTSVRVL SVDMVRWLRT 250 260 270 280 290 300 QKGLNIYVEP RVKEELLSES SSFNFVQTWE DDKEISLLHT KVDLLITLGG DGTVLWAASM 310 320 330 340 350 360 FKGPVPPIVP FSMGSLGFMT PFHSEQYRDC LEAILKGPIS ITLRHRLQCH IIRDKATHEY 370 380 390 400 410 420 EPEETMLVLN EVTIDRGISS YLTNLECYCD NSFVTCVQGD GLILSTTSGS TAYSLAAGGS 430 440 450 460 470 480 MVHPQVPGIL FTPICPHSLS FRPLILPEHV TVRVQVPFNS RSSAWVSFDG KDRKQLEAGD 490 500 510 520 ALVCSMAPWP VSTACQVEST NDFLRSIHDG LHWNLRKTQS ADGP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Stress induces the expression of AtNADK-1, a gene encoding a NAD(H) kinase in Arabidopsis thaliana." Berrin J.-G., Pierrugues O., Brutesco C., Alonso B., Montillet J.-L., Roby D., Kazmaier M. Mol. Genet. Genomics 273:10-19(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, TISSUE SPECIFICITY.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Cloning and characterization of two NAD kinases from Arabidopsis. Identification of a calmodulin binding isoform." Turner W.L., Waller J.C., Vanderbeld B., Snedden W.A. Plant Physiol. 135:1243-1255(2004) [PubMed] [Europe PMC] [Abstract] Cited for: BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY383545 mRNA. Translation: AAR32133.1. AP000604 Genomic DNA. Translation: BAB01450.1. Sequence problems. CP002686 Genomic DNA. Translation: AEE76456.1. AY090937 mRNA. Translation: AAM13987.1. Different initiation. AK176132 mRNA. Translation: BAD43895.1. AK221288 mRNA. Translation: BAD94003.1.
IPI	IPI00531291.
RefSeq	NP_974347.1. NM_202618.2.
UniGene	At.5579. At.72877.
3D structure databases
ProteinModelPortal	Q56YN3.
SMR	Q56YN3. Positions 207-512.
ModBase	Search...
Proteomic databases
PaxDb	Q56YN3.
PRIDE	Q56YN3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G21070.2; AT3G21070.2; AT3G21070.
GeneID	821659.
KEGG	ath:AT3G21070.
Organism-specific databases
TAIR	At3g21070.
Phylogenomic databases
eggNOG	COG0061.
HOGENOM	HOG000029806.
InParanoid	Q56YN3.
KO	K00858.
PhylomeDB	Q56YN3.
ProtClustDB	PLN02935.
Enzyme and pathway databases
BRENDA	2.7.1.86. 399.
Gene expression databases
ArrayExpress	Q56YN3.
Genevestigator	Q56YN3.
Family and domain databases
Gene3D	2.60.200.30. 1 hit. 3.40.50.10330. 1 hit.
InterPro	IPR017438. ATP-NAD_kinase_dom_1. IPR016064. ATP-NAD_kinase_PpnK-typ. IPR017437. ATP-NAD_kinase_PpnK-typ_all-b. IPR002504. PolyP/ATP_NADK_prd. [Graphical view]
PANTHER	PTHR20275. PTHR20275. 1 hit.
Pfam	PF01513. NAD_kinase. 1 hit. [Graphical view]
SUPFAM	SSF111331. ATP-NAD_kinase_PpnK-typ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADK1_ARATH

Accession

Primary (citable) accession number: Q56YN3
Secondary accession number(s): Q6TXT3, Q8RX27, Q9LJC5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	May 2, 2006
Last modified:	May 1, 2013
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program