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Protein

Transcription factor FAMA

Gene

FAMA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription activator (PubMed:17088607, PubMed:17183265, PubMed:17183267). Together with MYB88 and MYB124, ensures that stomata contain just two guard cells (GCs) by enforcing a single symmetric precursor cell division before stomatal maturity (PubMed:24571519). Together with SPCH and MUTE, regulates the stomata formation. Required to promote differentiation and morphogenesis of stomatal guard cells and to halt proliferative divisions in their immediate precursors. Mediates the formation of stomata (PubMed:17088607, PubMed:17183265, PubMed:17183267). Prevents histone H3K27me3 marks and derepresses stem cell gene expression (PubMed:24654956).5 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • guard cell differentiation Source: TAIR
  • guard cell fate commitment Source: UniProtKB
  • guard mother cell differentiation Source: UniProtKB
  • negative regulation of cell division Source: TAIR
  • negative regulation of histone H3-K27 methylation Source: UniProtKB
  • positive regulation of cell differentiation Source: TAIR
  • positive regulation of transcription, DNA-templated Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor FAMA
Alternative name(s):
Basic helix-loop-helix protein 97
Short name:
AtbHLH97
Short name:
bHLH 97
Transcription factor EN 14
bHLH transcription factor bHLH097
Gene namesi
Name:FAMA
Synonyms:BHLH97, EN14, FMA
Ordered Locus Names:At3g24140
ORF Names:MUJ8.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRilocus:2093746. AT3G24140.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  • nucleus Source: TAIR

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Excess symmetric divisions during stomata development leading to abnormal guard cells clusters formation. Reduced seeds set and low biomass. These phenotypes are complemented by MYB124.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi251 – 253CLE → GLK: Abolishes interaction with RBR1 that leads to a stomatal lineage-specific loss of terminal commitment. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003588431 – 414Transcription factor FAMAAdd BLAST414

Proteomic databases

PaxDbiQ56YJ8.
PRIDEiQ56YJ8.

Expressioni

Tissue specificityi

Resctricted to stomatal cell lineages (at protein level). Expressed in roots, leaves, stems, and flowers.2 Publications

Developmental stagei

Not expressed in meristemoids, but strongly expressed in guard mother cells (GMCs) and in young guard cells (at protein level) (PubMed:17088607). Expressed at the transition to terminal stomatal differentiation, just before and after the symmetric division of stomatal differentiation, being confined to late-stage GMC and to young, still differentiating guard cells (PubMed:24571519).2 Publications

Gene expression databases

ExpressionAtlasiQ56YJ8. baseline and differential.
GenevisibleiQ56YJ8. AT.

Interactioni

Subunit structurei

Interacts with FAMA through its LxCxE motif (PubMed:25303364). Self-interacts. Interacts also with bHLH071 and bHLH093 (PubMed:17088607). Interacts with RBR1 (PubMed:24571519).3 Publications

Protein-protein interaction databases

BioGridi7332. 19 interactors.
IntActiQ56YJ8. 15 interactors.
STRINGi3702.AT3G24140.1.

Structurei

3D structure databases

ProteinModelPortaliQ56YJ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini194 – 245bHLHPROSITE-ProRule annotationAdd BLAST52

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi249 – 253LxCxE motif1 Publication5

Phylogenomic databases

eggNOGiENOG410IIZK. Eukaryota.
ENOG410YBHA. LUCA.
HOGENOMiHOG000238962.
InParanoidiQ56YJ8.
OMAiHANTNIS.
OrthoDBiEOG09360J1S.
PhylomeDBiQ56YJ8.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiView protein in InterPro
IPR011598. bHLH_dom.
PfamiView protein in Pfam
PF00010. HLH. 1 hit.
SMARTiView protein in SMART
SM00353. HLH. 1 hit.
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiView protein in PROSITE
PS50888. BHLH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q56YJ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKDYSAPNF LGESSGGNDD NSSGMIDYMF NRNLQQQQKQ SMPQQQQHQL
60 70 80 90 100
SPSGFGATPF DKMNFSDVMQ FADFGSKLAL NQTRNQDDQE TGIDPVYFLK
110 120 130 140 150
FPVLNDKIED HNQTQHLMPS HQTSQEGGEC GGNIGNVFLE EKEDQDDDND
160 170 180 190 200
NNSVQLRFIG GEEEDRENKN VTKKEVKSKR KRARTSKTSE EVESQRMTHI
210 220 230 240 250
AVERNRRKQM NEHLRVLRSL MPGSYVQRGD QASIIGGAIE FVRELEQLLQ
260 270 280 290 300
CLESQKRRRI LGETGRDMTT TTTSSSSPIT TVANQAQPLI ITGNVTELEG
310 320 330 340 350
GGGLREETAE NKSCLADVEV KLLGFDAMIK ILSRRRPGQL IKTIAALEDL
360 370 380 390 400
HLSILHTNIT TMEQTVLYSF NVKITSETRF TAEDIASSIQ QIFSFIHANT
410
NISGSSNLGN IVFT
Length:414
Mass (Da):46,408
Last modified:May 10, 2005 - v1
Checksum:i70F6B5DC0732B1B2
GO

Sequence cautioni

The sequence BAB01355 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028621 Genomic DNA. Translation: BAB01355.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE76862.1.
AK221324 mRNA. Translation: BAD94119.1.
BT028961 mRNA. Translation: ABI54336.1.
AF488624 mRNA. No translation available.
RefSeqiNP_189056.2. NM_113319.5.
UniGeneiAt.37586.

Genome annotation databases

EnsemblPlantsiAT3G24140.1; AT3G24140.1; AT3G24140.
GeneIDi822000.
GrameneiAT3G24140.1; AT3G24140.1; AT3G24140.
KEGGiath:AT3G24140.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028621 Genomic DNA. Translation: BAB01355.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE76862.1.
AK221324 mRNA. Translation: BAD94119.1.
BT028961 mRNA. Translation: ABI54336.1.
AF488624 mRNA. No translation available.
RefSeqiNP_189056.2. NM_113319.5.
UniGeneiAt.37586.

3D structure databases

ProteinModelPortaliQ56YJ8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7332. 19 interactors.
IntActiQ56YJ8. 15 interactors.
STRINGi3702.AT3G24140.1.

Proteomic databases

PaxDbiQ56YJ8.
PRIDEiQ56YJ8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G24140.1; AT3G24140.1; AT3G24140.
GeneIDi822000.
GrameneiAT3G24140.1; AT3G24140.1; AT3G24140.
KEGGiath:AT3G24140.

Organism-specific databases

AraportiAT3G24140.
TAIRilocus:2093746. AT3G24140.

Phylogenomic databases

eggNOGiENOG410IIZK. Eukaryota.
ENOG410YBHA. LUCA.
HOGENOMiHOG000238962.
InParanoidiQ56YJ8.
OMAiHANTNIS.
OrthoDBiEOG09360J1S.
PhylomeDBiQ56YJ8.

Miscellaneous databases

PROiQ56YJ8.

Gene expression databases

ExpressionAtlasiQ56YJ8. baseline and differential.
GenevisibleiQ56YJ8. AT.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiView protein in InterPro
IPR011598. bHLH_dom.
PfamiView protein in Pfam
PF00010. HLH. 1 hit.
SMARTiView protein in SMART
SM00353. HLH. 1 hit.
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiView protein in PROSITE
PS50888. BHLH. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFAMA_ARATH
AccessioniPrimary (citable) accession number: Q56YJ8
Secondary accession number(s): Q9LRN3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: May 10, 2005
Last modified: February 15, 2017
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plants overexpressing FAMA exhibit ectopic stomata.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.