Serine--glyoxylate aminotransferase - Arabidopsis thaliana (Mouse-ear cress)

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Q56YA5 (SGAT_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 66. History...

Clusters with 100%, 90%, 50% identity |

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Serine--glyoxylate aminotransferase
EC=2.6.1.45

Alternative name(s):
Alanine--glyoxylate aminotransferase
Short name=AGT
EC=2.6.1.44
Serine--pyruvate aminotransferase
EC=2.6.1.51

Gene names

Name:	AGT1
Ordered Locus Names:	At2g13360
ORF Names:	F14O4.7

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Photorespiratory enzyme that catalyzes transamination reactions with multiple substrates.
Catalytic activity	L-serine + glyoxylate = 3-hydroxypyruvate + glycine. L-alanine + glyoxylate = pyruvate + glycine. L-serine + pyruvate = 3-hydroxypyruvate + L-alanine.
Cofactor	Pyridoxal phosphate.
Subunit structure	Homodimer.
Subcellular location	Peroxisome Ref.7.
Tissue specificity	Ubiquitous. Preferentially expressed in green, leafy tissues. Ref.7
Miscellaneous	Preferentially acts as a serine--glyoxylate aminotransferase in vitro.
Sequence similarities	Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Photorespiration
Cellular component	Peroxisome
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	photorespiration Non-traceable author statement Ref.7. Source: TAIR
Cellular_component	apoplast Inferred from direct assay PubMed 18538804. Source: TAIR chloroplast stroma Inferred from direct assay PubMed 18633119 PubMed 20061580. Source: TAIR peroxisome Inferred from direct assay Ref.7 PubMed 17951448. Source: TAIR plasma membrane Inferred from direct assay Ref.8. Source: TAIR
Molecular_function	alanine-glyoxylate transaminase activity Inferred from direct assay Ref.7. Source: TAIR pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro serine-glyoxylate transaminase activity Inferred from direct assay Ref.7. Source: TAIR serine-pyruvate transaminase activity Inferred from direct assay Ref.7. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 401

401

Serine--glyoxylate aminotransferase

PRO_0000150234

Regions

Motif

399 – 401

Microbody targeting signal Potential

Amino acid modifications

Modified residue

201

N6-(pyridoxal phosphate)lysine By similarity

Modified residue

330

Phosphotyrosine Ref.8

Experimental info

Mutagenesis

251

P → L: Abolishes aminotransferase activity. Ref.7

Sequences

Sequence

Length

Mass (Da)

Tools

Q56YA5 [UniParc].

Last modified September 13, 2005. Version 2.
Checksum: 608E7EFD840C84D3
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FASTA

401

44,208

        10         20         30         40         50         60 
MDYMYGPGRH HLFVPGPVNI PEPVIRAMNR NNEDYRSPAI PALTKTLLED VKKIFKTTSG 

        70         80         90        100        110        120 
TPFLFPTTGT GAWESALTNT LSPGDRIVSF LIGQFSLLWI DQQKRLNFNV DVVESDWGQG 

       130        140        150        160        170        180 
ANLQVLASKL SQDENHTIKA ICIVHNETAT GVTNDISAVR TLLDHYKHPA LLLVDGVSSI 

       190        200        210        220        230        240 
CALDFRMDEW GVDVALTGSQ KALSLPTGLG IVCASPKALE ATKTSKSLKV FFDWNDYLKF 

       250        260        270        280        290        300 
YKLGTYWPYT PSIQLLYGLR AALDLIFEEG LENIIARHAR LGKATRLAVE AWGLKNCTQK 

       310        320        330        340        350        360 
EEWISNTVTA VMVPPHIDGS EIVRRAWQRY NLSLGLGLNK VAGKVFRIGH LGNVNELQLL 

       370        380        390        400 
GCLAGVEMIL KDVGYPVVMG SGVAAASTYL QHHIPLIPSR I

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a cDNA encoding alanine:glyoxylate aminotransferase from Arabidopsis thaliana." Liepman A.H., Olsen L.J. Plant Gene Register PGR98-113 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Molecular characterization of serine:glyoxylate aminotransferase localized in plant leaf peroxisomes." Yamaguchi K., Takeuchi Y., Nishimura M. Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 278-401. Strain: cv. Columbia.
[7]	"Peroxisomal alanine:glyoxylate aminotransferase (AGT1) is a photorespiratory enzyme with multiple substrates in Arabidopsis thaliana." Liepman A.H., Olsen L.J. Plant J. 25:487-498(2001) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, MUTAGENESIS OF PRO-251.
[8]	"Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry." Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C. Mol. Cell. Proteomics 2:1234-1243(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-330, MASS SPECTROMETRY. Strain: cv. La-0.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF063901 mRNA. Translation: AAC26854.1. AB048945 mRNA. Translation: BAB20811.1. AC007209 Genomic DNA. Translation: AAD28669.1. CP002685 Genomic DNA. Translation: AEC06227.1. CP002685 Genomic DNA. Translation: AEC06228.1. AY096498 mRNA. Translation: AAM20136.1. AY114010 mRNA. Translation: AAM45058.1. AK221418 mRNA. Translation: BAD94403.1.
IPI	IPI00540396.
PIR	T52250.
RefSeq	NP_178969.1. NM_126925.4. NP_849951.1. NM_179620.2.
UniGene	At.24853.
3D structure databases
ProteinModelPortal	Q56YA5.
SMR	Q56YA5. Positions 12-352.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q56YA5. 3 interactions.
Proteomic databases
PaxDb	Q56YA5.
PRIDE	Q56YA5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G13360.1; AT2G13360.1; AT2G13360. AT2G13360.2; AT2G13360.2; AT2G13360.
GeneID	815822.
KEGG	ath:AT2G13360.
Organism-specific databases
GeneFarm	5080.
TAIR	At2g13360.
Phylogenomic databases
eggNOG	COG0075.
HOGENOM	HOG000171814.
InParanoid	Q56YA5.
KO	K00830.
OMA	RINHMGI.
PhylomeDB	Q56YA5.
ProtClustDB	PLN02409.
Enzyme and pathway databases
BioCyc	MetaCyc:AT2G13360-MONOMER.
BRENDA	2.6.1.44. 399.
SABIO-RK	Q56YA5.
Gene expression databases
Genevestigator	Q56YA5.
GermOnline	AT2G13360. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 1 hit.
InterPro	IPR000192. Aminotrans_V/Cys_dSase. IPR020578. Aminotrans_V_PyrdxlP_BS. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. IPR024169. SP_NH2Trfase/AEP_transaminase. [Graphical view]
Pfam	PF00266. Aminotran_5. 1 hit. [Graphical view]
PIRSF	PIRSF000524. SPT. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00595. AA_TRANSFER_CLASS_5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SGAT_ARATH

Accession

Primary (citable) accession number: Q56YA5
Secondary accession number(s): O81248

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	September 13, 2005
Last modified:	May 1, 2013
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents