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Protein

Methionine aminopeptidase 2B

Gene

MAP2B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei192SubstrateUniRule annotation1
Metal bindingi212Divalent metal cation 1UniRule annotation1
Metal bindingi223Divalent metal cation 1UniRule annotation1
Metal bindingi223Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi292Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation1
Binding sitei300SubstrateUniRule annotation1
Metal bindingi325Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi420Divalent metal cation 1UniRule annotation1
Metal bindingi420Divalent metal cation 2; catalyticUniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • protein processing Source: TAIR

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease
LigandMetal-binding

Enzyme and pathway databases

ReactomeiR-ATH-2514859 Inactivation, recovery and regulation of the phototransduction cascade

Protein family/group databases

MEROPSiM24.A02

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidase 2BUniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAP 2BUniRule annotation
Short name:
MetAP 2BUniRule annotation
Alternative name(s):
Peptidase MUniRule annotation
Gene namesi
Name:MAP2BUniRule annotation
Ordered Locus Names:At3g59990
ORF Names:F24G16.26
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G59990
TAIRilocus:2080507 AT3G59990

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001489711 – 439Methionine aminopeptidase 2BAdd BLAST439

Proteomic databases

PaxDbiQ56Y85
PRIDEiQ56Y85

Expressioni

Tissue specificityi

Ubiquitous. Preferentially expressed in roots.1 Publication

Gene expression databases

ExpressionAtlasiQ56Y85 baseline and differential
GenevisibleiQ56Y85 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G59990.1

Structurei

3D structure databases

ProteinModelPortaliQ56Y85
SMRiQ56Y85
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi61 – 73Lys-richAdd BLAST13

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2775 Eukaryota
COG0024 LUCA
HOGENOMiHOG000226278
InParanoidiQ56Y85
KOiK01265
OMAiTINKHFG
OrthoDBiEOG093608Q0
PhylomeDBiQ56Y85

Family and domain databases

CDDicd01088 MetAP2, 1 hit
Gene3Di1.10.10.10, 1 hit
HAMAPiMF_03175 MetAP_2_euk, 1 hit
InterProiView protein in InterPro
IPR036005 Creatinase/aminopeptidase-like
IPR000994 Pept_M24
IPR001714 Pept_M24_MAP
IPR002468 Pept_M24A_MAP2
IPR018349 Pept_M24A_MAP2_BS
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00557 Peptidase_M24, 1 hit
PRINTSiPR00599 MAPEPTIDASE
SUPFAMiSSF46785 SSF46785, 1 hit
SSF55920 SSF55920, 2 hits
TIGRFAMsiTIGR00501 met_pdase_II, 1 hit
PROSITEiView protein in PROSITE
PS01202 MAP_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q56Y85-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASESPDVAV VAPVVENGGA ESSNGKEEQL ESELSKKLEI AEDGQEENDG
60 70 80 90 100
EEGSKAETST KKKKKKNKSK KKKELPQQTD PPSIPVVELF PSGEFPEGEI
110 120 130 140 150
QEYKDDNLWR TTSEEKRELE RFEKPIYNSV RRAAEVHRQV RKYVRSIVKP
160 170 180 190 200
GMLMTDICET LENTVRKLIS ENGLQAGIAF PTGCSLNWVA AHWTPNSGDK
210 220 230 240 250
TVLQYDDVMK LDFGTHIDGH IIDCAFTVAF NPMFDPLLAA SREATYTGIK
260 270 280 290 300
EAGIDVRLCD IGAAIQEVME SYEVEINGKV FQVKSIRNLN GHSIGPYQIH
310 320 330 340 350
AGKSVPIVKG GEQTKMEEGE FYAIETFGST GKGYVREDLE CSHYMKNFDA
360 370 380 390 400
GHVPLRLPRA KQLLATINKN FSTLAFCRRY LDRIGETKYL MALKNLCDSG
410 420 430
IVQPYPPLCD VKGSYVSQFE HTILLRPTCK EVLSKGDDY
Length:439
Mass (Da):49,060
Last modified:January 24, 2006 - v2
Checksum:iEC1C79EA1B49D3BF
GO

Sequence cautioni

The sequence BAD94472 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAB75818 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti270E → K in AAL38337 (PubMed:14593172).Curated1
Sequence conflicti270E → K in AAN15382 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF300880 mRNA Translation: AAG34551.1
AL138647 Genomic DNA Translation: CAB75818.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE79996.1
CP002686 Genomic DNA Translation: AEE79997.1
CP002686 Genomic DNA Translation: AEE79998.1
CP002686 Genomic DNA Translation: AEE79999.1
AY065161 mRNA Translation: AAL38337.1
BT000063 mRNA Translation: AAN15382.1
AY084710 mRNA Translation: AAM61284.1
AK221438 mRNA Translation: BAD94472.1 Different initiation.
PIRiT47823
RefSeqiNP_001030898.1, NM_001035821.1
NP_001190139.1, NM_001203210.1
NP_567089.1, NM_115862.3
NP_850725.1, NM_180394.2
UniGeneiAt.16912

Genome annotation databases

EnsemblPlantsiAT3G59990.1; AT3G59990.1; AT3G59990
AT3G59990.2; AT3G59990.2; AT3G59990
AT3G59990.3; AT3G59990.3; AT3G59990
AT3G59990.4; AT3G59990.4; AT3G59990
GeneIDi825169
GrameneiAT3G59990.1; AT3G59990.1; AT3G59990
AT3G59990.2; AT3G59990.2; AT3G59990
AT3G59990.3; AT3G59990.3; AT3G59990
AT3G59990.4; AT3G59990.4; AT3G59990
KEGGiath:AT3G59990

Similar proteinsi

Entry informationi

Entry nameiMAP22_ARATH
AccessioniPrimary (citable) accession number: Q56Y85
Secondary accession number(s): Q8VZ89, Q9FPV8, Q9M1X7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: January 24, 2006
Last modified: April 25, 2018
This is version 100 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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