Q56Y85 (AMP2B_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine aminopeptidase 2B
Short name=MAP 2B
Short name=MetAP 2B
EC=3.4.11.18

Alternative name(s):
Peptidase M 2B

Gene names

Name:	MAP2B
Ordered Locus Names:	At3g59990
ORF Names:	F24G16.26

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity.
Subcellular location	Cytoplasm Ref.1.
Tissue specificity	Ubiquitous. Preferentially expressed in roots. Ref.1
Sequence similarities	Belongs to the peptidase M24A family.
Sequence caution	The sequence BAD94472.1 differs from that shown. Reason: Erroneous initiation. The sequence CAB75818.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro protein processing Traceable author statement Ref.1. Source: TAIR proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay Ref.1. Source: TAIR
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 439	439	Methionine aminopeptidase 2B		PRO_0000148971
Regions
	Compositional bias	61 – 73	13	Lys-rich
Sites
	Metal binding	212	1	Cobalt 1 By similarity
	Metal binding	223	1	Cobalt 1 By similarity
	Metal binding	223	1	Cobalt 2 By similarity
	Metal binding	292	1	Cobalt 2 By similarity
	Metal binding	325	1	Cobalt 2 By similarity
	Metal binding	420	1	Cobalt 1 By similarity
	Metal binding	420	1	Cobalt 2 By similarity
	Binding site	192	1	Substrate By similarity
	Binding site	300	1	Substrate By similarity
Experimental info
	Sequence conflict	270	1	E → K in AAL38337. Ref.4
	Sequence conflict	270	1	E → K in AAN15382. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q56Y85 [UniParc]. Last modified January 24, 2006. Version 2. Checksum: EC1C79EA1B49D3BF	FASTA	439	49,060
	10 20 30 40 50 60 MASESPDVAV VAPVVENGGA ESSNGKEEQL ESELSKKLEI AEDGQEENDG EEGSKAETST 70 80 90 100 110 120 KKKKKKNKSK KKKELPQQTD PPSIPVVELF PSGEFPEGEI QEYKDDNLWR TTSEEKRELE 130 140 150 160 170 180 RFEKPIYNSV RRAAEVHRQV RKYVRSIVKP GMLMTDICET LENTVRKLIS ENGLQAGIAF 190 200 210 220 230 240 PTGCSLNWVA AHWTPNSGDK TVLQYDDVMK LDFGTHIDGH IIDCAFTVAF NPMFDPLLAA 250 260 270 280 290 300 SREATYTGIK EAGIDVRLCD IGAAIQEVME SYEVEINGKV FQVKSIRNLN GHSIGPYQIH 310 320 330 340 350 360 AGKSVPIVKG GEQTKMEEGE FYAIETFGST GKGYVREDLE CSHYMKNFDA GHVPLRLPRA 370 380 390 400 410 420 KQLLATINKN FSTLAFCRRY LDRIGETKYL MALKNLCDSG IVQPYPPLCD VKGSYVSQFE 430 HTILLRPTCK EVLSKGDDY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of eukaryotic peptide deformylases reveals universality of N-terminal protein processing mechanisms." Giglione C., Serero A., Pierre M., Boisson B., Meinnel T. EMBO J. 19:5916-5929(2000) [PubMed: 11060042] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[2]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 263-439. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF300880 mRNA. Translation: AAG34551.1. AL138647 Genomic DNA. Translation: CAB75818.1. Sequence problems. CP002686 Genomic DNA. Translation: AEE79996.1. CP002686 Genomic DNA. Translation: AEE79997.1. CP002686 Genomic DNA. Translation: AEE79998.1. CP002686 Genomic DNA. Translation: AEE79999.1. AY065161 mRNA. Translation: AAL38337.1. BT000063 mRNA. Translation: AAN15382.1. AY084710 mRNA. Translation: AAM61284.1. AK221438 mRNA. Translation: BAD94472.1. Different initiation.
IPI	IPI00536551.
PIR	T47823.
RefSeq	NP_001030898.1. NM_001035821.1. NP_001190139.1. NM_001203210.1. NP_567089.1. NM_115862.3. NP_850725.1. NM_180394.1.
UniGene	At.16912.
3D structure databases
ProteinModelPortal	Q56Y85.
SMR	Q56Y85. Positions 78-439.
ModBase	Search...
Protein family/group databases
MEROPS	M24.002.
Proteomic databases
PRIDE	Q56Y85.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G59990.1; AT3G59990.1; AT3G59990. AT3G59990.2; AT3G59990.2; AT3G59990. AT3G59990.3; AT3G59990.3; AT3G59990. AT3G59990.4; AT3G59990.4; AT3G59990.
GeneID	825169.
GenomeReviews	Gene locus AT3G59990 in contig BA000014_GR.
KEGG	ath:AT3G59990.
NMPDR	fig|3702.1.peg.17359.
Organism-specific databases
GeneFarm	1944. 191.
TAIR	At3g59990.
Phylogenomic databases
eggNOG	KOG2775.
GeneTree	EPGT00070000029106.
HOGENOM	HBG318153.
InParanoid	Q56Y85.
OMA	VKGCYTA.
PhylomeDB	Q56Y85.
ProtClustDB	CLSN2688923.
Gene expression databases
Genevestigator	Q56Y85.
GermOnline	AT3G59990. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002468. Pept_M24A_MAP2. IPR018349. Pept_M24A_MAP2_BS. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 2 hits. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K01265.
PANTHER	PTHR10804:SF9. Pept_M24A_MAP2. 1 hit.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
SUPFAM	SSF55920. Peptidase_M24_cat_core. 1 hit.
TIGRFAMs	TIGR00501. Met_pdase_II. 1 hit.
PROSITE	PS01202. MAP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMP2B_ARATH

Accession

Primary (citable) accession number: Q56Y85
Secondary accession number(s): Q8VZ89, Q9FPV8, Q9M1X7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	January 24, 2006
Last modified:	November 16, 2011
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program