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Protein

Methionine aminopeptidase 2B

Gene

MAP2B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei192SubstrateUniRule annotation1
Metal bindingi212Divalent metal cation 1UniRule annotation1
Metal bindingi223Divalent metal cation 1UniRule annotation1
Metal bindingi223Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi292Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation1
Binding sitei300SubstrateUniRule annotation1
Metal bindingi325Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi420Divalent metal cation 1UniRule annotation1
Metal bindingi420Divalent metal cation 2; catalyticUniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • protein initiator methionine removal Source: UniProtKB-HAMAP
  • protein processing Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Metal-binding

Protein family/group databases

MEROPSiM24.A02.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidase 2BUniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAP 2BUniRule annotation
Short name:
MetAP 2BUniRule annotation
Alternative name(s):
Peptidase MUniRule annotation
Gene namesi
Name:MAP2BUniRule annotation
Ordered Locus Names:At3g59990
ORF Names:F24G16.26
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G59990.

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

GO - Cellular componenti

  • cytoplasm Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001489711 – 439Methionine aminopeptidase 2BAdd BLAST439

Proteomic databases

PaxDbiQ56Y85.
PRIDEiQ56Y85.

Expressioni

Tissue specificityi

Ubiquitous. Preferentially expressed in roots.1 Publication

Gene expression databases

GenevisibleiQ56Y85. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G59990.1.

Structurei

3D structure databases

ProteinModelPortaliQ56Y85.
SMRiQ56Y85.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi61 – 73Lys-richAdd BLAST13

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2775. Eukaryota.
COG0024. LUCA.
HOGENOMiHOG000226278.
InParanoidiQ56Y85.
KOiK01265.
OMAiWEHTILL.
OrthoDBiEOG093608Q0.
PhylomeDBiQ56Y85.

Family and domain databases

CDDicd01088. MetAP2. 1 hit.
Gene3Di1.10.10.10. 1 hit.
3.90.230.10. 2 hits.
HAMAPiMF_03175. MetAP_2_euk. 1 hit.
InterProiIPR000994. Pept_M24.
IPR001714. Pept_M24_MAP.
IPR002468. Pept_M24A_MAP2.
IPR018349. Pept_M24A_MAP2_BS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10804:SF9. PTHR10804:SF9. 1 hit.
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF55920. SSF55920. 2 hits.
TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.
PROSITEiPS01202. MAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q56Y85-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASESPDVAV VAPVVENGGA ESSNGKEEQL ESELSKKLEI AEDGQEENDG
60 70 80 90 100
EEGSKAETST KKKKKKNKSK KKKELPQQTD PPSIPVVELF PSGEFPEGEI
110 120 130 140 150
QEYKDDNLWR TTSEEKRELE RFEKPIYNSV RRAAEVHRQV RKYVRSIVKP
160 170 180 190 200
GMLMTDICET LENTVRKLIS ENGLQAGIAF PTGCSLNWVA AHWTPNSGDK
210 220 230 240 250
TVLQYDDVMK LDFGTHIDGH IIDCAFTVAF NPMFDPLLAA SREATYTGIK
260 270 280 290 300
EAGIDVRLCD IGAAIQEVME SYEVEINGKV FQVKSIRNLN GHSIGPYQIH
310 320 330 340 350
AGKSVPIVKG GEQTKMEEGE FYAIETFGST GKGYVREDLE CSHYMKNFDA
360 370 380 390 400
GHVPLRLPRA KQLLATINKN FSTLAFCRRY LDRIGETKYL MALKNLCDSG
410 420 430
IVQPYPPLCD VKGSYVSQFE HTILLRPTCK EVLSKGDDY
Length:439
Mass (Da):49,060
Last modified:January 24, 2006 - v2
Checksum:iEC1C79EA1B49D3BF
GO

Sequence cautioni

The sequence BAD94472 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAB75818 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti270E → K in AAL38337 (PubMed:14593172).Curated1
Sequence conflicti270E → K in AAN15382 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF300880 mRNA. Translation: AAG34551.1.
AL138647 Genomic DNA. Translation: CAB75818.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79996.1.
CP002686 Genomic DNA. Translation: AEE79997.1.
CP002686 Genomic DNA. Translation: AEE79998.1.
CP002686 Genomic DNA. Translation: AEE79999.1.
AY065161 mRNA. Translation: AAL38337.1.
BT000063 mRNA. Translation: AAN15382.1.
AY084710 mRNA. Translation: AAM61284.1.
AK221438 mRNA. Translation: BAD94472.1. Different initiation.
PIRiT47823.
RefSeqiNP_001030898.1. NM_001035821.1.
NP_001190139.1. NM_001203210.1.
NP_567089.1. NM_115862.3.
NP_850725.1. NM_180394.2.
UniGeneiAt.16912.

Genome annotation databases

EnsemblPlantsiAT3G59990.1; AT3G59990.1; AT3G59990.
AT3G59990.2; AT3G59990.2; AT3G59990.
AT3G59990.3; AT3G59990.3; AT3G59990.
AT3G59990.4; AT3G59990.4; AT3G59990.
GeneIDi825169.
GrameneiAT3G59990.1; AT3G59990.1; AT3G59990.
AT3G59990.2; AT3G59990.2; AT3G59990.
AT3G59990.3; AT3G59990.3; AT3G59990.
AT3G59990.4; AT3G59990.4; AT3G59990.
KEGGiath:AT3G59990.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF300880 mRNA. Translation: AAG34551.1.
AL138647 Genomic DNA. Translation: CAB75818.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79996.1.
CP002686 Genomic DNA. Translation: AEE79997.1.
CP002686 Genomic DNA. Translation: AEE79998.1.
CP002686 Genomic DNA. Translation: AEE79999.1.
AY065161 mRNA. Translation: AAL38337.1.
BT000063 mRNA. Translation: AAN15382.1.
AY084710 mRNA. Translation: AAM61284.1.
AK221438 mRNA. Translation: BAD94472.1. Different initiation.
PIRiT47823.
RefSeqiNP_001030898.1. NM_001035821.1.
NP_001190139.1. NM_001203210.1.
NP_567089.1. NM_115862.3.
NP_850725.1. NM_180394.2.
UniGeneiAt.16912.

3D structure databases

ProteinModelPortaliQ56Y85.
SMRiQ56Y85.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G59990.1.

Protein family/group databases

MEROPSiM24.A02.

Proteomic databases

PaxDbiQ56Y85.
PRIDEiQ56Y85.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G59990.1; AT3G59990.1; AT3G59990.
AT3G59990.2; AT3G59990.2; AT3G59990.
AT3G59990.3; AT3G59990.3; AT3G59990.
AT3G59990.4; AT3G59990.4; AT3G59990.
GeneIDi825169.
GrameneiAT3G59990.1; AT3G59990.1; AT3G59990.
AT3G59990.2; AT3G59990.2; AT3G59990.
AT3G59990.3; AT3G59990.3; AT3G59990.
AT3G59990.4; AT3G59990.4; AT3G59990.
KEGGiath:AT3G59990.

Organism-specific databases

TAIRiAT3G59990.

Phylogenomic databases

eggNOGiKOG2775. Eukaryota.
COG0024. LUCA.
HOGENOMiHOG000226278.
InParanoidiQ56Y85.
KOiK01265.
OMAiWEHTILL.
OrthoDBiEOG093608Q0.
PhylomeDBiQ56Y85.

Miscellaneous databases

PROiQ56Y85.

Gene expression databases

GenevisibleiQ56Y85. AT.

Family and domain databases

CDDicd01088. MetAP2. 1 hit.
Gene3Di1.10.10.10. 1 hit.
3.90.230.10. 2 hits.
HAMAPiMF_03175. MetAP_2_euk. 1 hit.
InterProiIPR000994. Pept_M24.
IPR001714. Pept_M24_MAP.
IPR002468. Pept_M24A_MAP2.
IPR018349. Pept_M24A_MAP2_BS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10804:SF9. PTHR10804:SF9. 1 hit.
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF55920. SSF55920. 2 hits.
TIGRFAMsiTIGR00501. met_pdase_II. 1 hit.
PROSITEiPS01202. MAP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAP22_ARATH
AccessioniPrimary (citable) accession number: Q56Y85
Secondary accession number(s): Q8VZ89, Q9FPV8, Q9M1X7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: January 24, 2006
Last modified: November 30, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.