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Protein

Hexokinase-4

Gene

At3g20040

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Fructose and glucose phosphorylating enzyme. May be involved in the phosphorylation of glucose during the export from mitochondrion to cytosol (By similarity).By similarity

Catalytic activityi

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi101 – 1066ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hexokinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G20040-MONOMER.
ReactomeiREACT_234283. Regulation of Glucokinase by Glucokinase Regulatory Protein.
REACT_239741. Regulation of gene expression in beta cells.
REACT_253266. Glucose transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Hexokinase-4 (EC:2.7.1.1)
Gene namesi
Ordered Locus Names:At3g20040
ORF Names:MAL21.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G20040.

Subcellular locationi

Mitochondrion outer membrane 1 Publication; Single-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei4 – 2421HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of mitochondrial outer membrane Source: TAIR
  2. mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502Hexokinase-4PRO_0000259632Add
BLAST

Proteomic databases

PaxDbiQ56XE8.
PRIDEiQ56XE8.

Expressioni

Gene expression databases

GenevestigatoriQ56XE8.

Interactioni

Protein-protein interaction databases

BioGridi6875. 1 interaction.
STRINGi3702.AT3G20040.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ56XE8.
SMRiQ56XE8. Positions 62-491.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 235195Hexokinase type-1Add
BLAST
Domaini248 – 491244Hexokinase type-2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni171 – 19727Glucose-bindingSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the hexokinase family.Curated
Contains 1 hexokinase type-1 domain.Curated
Contains 1 hexokinase type-2 domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5026.
HOGENOMiHOG000162670.
InParanoidiQ56XE8.
KOiK00844.
OMAiFAISEMA.
PhylomeDBiQ56XE8.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PRINTSiPR00475. HEXOKINASE.

Sequencei

Sequence statusi: Complete.

Q56XE8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKVLVMLTA AAAVVACSVA TVMVRRRMKG RRKWRRVVGL LKDLEEACET
60 70 80 90 100
PLGRLRQMVD AIAVEMQAGL VSEGGSKLKM LLTFVDDLPN GSETGTYYAL
110 120 130 140 150
HLGGSYFRII KVHLGGQRSS LEVQDVERHS IPTSLMNSTS EVLFDFLASS
160 170 180 190 200
LQRFIEKEGN DFSLSQPLKR ELAFTFSFPV KQTSISSGVL IKWTKGFAIS
210 220 230 240 250
EMAGEDIAEC LQGALNKRGL DIRVAALVND TVGALSFGHF HDPDTIAAVV
260 270 280 290 300
FGTGSNACYL ERTDAIIKCQ NPRTTSGSMV VNMEWGNFWS SRLPRTSYDL
310 320 330 340 350
ELDAESMNSN DMGFEKMIGG MYLGDIVRRV ILRMSQESDI FGPISSILST
360 370 380 390 400
PFVLRTNSVS AMHEDDTSEL QEVARILKDL GVSEVPMKVR KLVVKICDVV
410 420 430 440 450
TRRAARLAAA GIAGILKKVG RDGSGGGRRS DKQIMRRTVV AVEGGLYLNY
460 470 480 490 500
RMFREYMDEA LRDILGEDVA QHVVVKAMED GSSIGSALLL ASSQSVQTIP

SV
Length:502
Mass (Da):54,955
Last modified:October 31, 2006 - v2
Checksum:i354D46773BA91199
GO

Sequence cautioni

The sequence BAD93730.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti502 – 5021V → I in BAD93730. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000383 Genomic DNA. Translation: BAB01861.1.
CP002686 Genomic DNA. Translation: AEE76323.1.
AK221726 mRNA. Translation: BAD93730.1. Different initiation.
RefSeqiNP_188639.2. NM_112895.2.
UniGeneiAt.50841.

Genome annotation databases

EnsemblPlantsiAT3G20040.1; AT3G20040.1; AT3G20040.
GeneIDi821543.
KEGGiath:AT3G20040.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000383 Genomic DNA. Translation: BAB01861.1.
CP002686 Genomic DNA. Translation: AEE76323.1.
AK221726 mRNA. Translation: BAD93730.1. Different initiation.
RefSeqiNP_188639.2. NM_112895.2.
UniGeneiAt.50841.

3D structure databases

ProteinModelPortaliQ56XE8.
SMRiQ56XE8. Positions 62-491.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6875. 1 interaction.
STRINGi3702.AT3G20040.1-P.

Proteomic databases

PaxDbiQ56XE8.
PRIDEiQ56XE8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G20040.1; AT3G20040.1; AT3G20040.
GeneIDi821543.
KEGGiath:AT3G20040.

Organism-specific databases

GeneFarmi4272. 430.
TAIRiAT3G20040.

Phylogenomic databases

eggNOGiCOG5026.
HOGENOMiHOG000162670.
InParanoidiQ56XE8.
KOiK00844.
OMAiFAISEMA.
PhylomeDBiQ56XE8.

Enzyme and pathway databases

BioCyciARA:AT3G20040-MONOMER.
ReactomeiREACT_234283. Regulation of Glucokinase by Glucokinase Regulatory Protein.
REACT_239741. Regulation of gene expression in beta cells.
REACT_253266. Glucose transport.

Gene expression databases

GenevestigatoriQ56XE8.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 1 hit.
PF03727. Hexokinase_2. 1 hit.
[Graphical view]
PRINTSiPR00475. HEXOKINASE.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 18-502.
    Strain: cv. Columbia.
  4. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
    Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
    Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Landsberg erecta.

Entry informationi

Entry nameiHXK4_ARATH
AccessioniPrimary (citable) accession number: Q56XE8
Secondary accession number(s): Q9LJZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 31, 2006
Last modified: January 7, 2015
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.