Reviewed, UniProtKB/Swiss-Prot Q56XE8 (HXK4_ARATH)

Names and origin

Protein names

Recommended name:
    Hexokinase-4
    EC=2.7.1.1

Gene names

Ordered Locus Names:	At3g20040
ORF Names:	MAL21.4

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Fructose and glucose phosphorylating enzyme. May be involved in the phosphorylation of glucose during the export from mitochondrion to cytosol By similarity.
Catalytic activity	ATP + D-hexose = ADP + D-hexose 6-phosphate.
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein Ref.3.
Sequence similarities	Belongs to the hexokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hexokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Hexokinase-4		PRO_0000259632
Regions
	Transmembrane	4 – 24	21	Potential
	Nucleotide binding	101 – 106	6	ATP Potential
	Region	171 – 197	27	Glucose-binding Potential
Experimental info
	Sequence conflict	502	1	V → I in BAD93730. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q56XE8-1 [UniParc]. Last modified October 31, 2006. Version 2. Checksum: 354D46773BA91199	FASTA	502	54,955
	10 20 30 40 50 60 MGKVLVMLTA AAAVVACSVA TVMVRRRMKG RRKWRRVVGL LKDLEEACET PLGRLRQMVD 70 80 90 100 110 120 AIAVEMQAGL VSEGGSKLKM LLTFVDDLPN GSETGTYYAL HLGGSYFRII KVHLGGQRSS 130 140 150 160 170 180 LEVQDVERHS IPTSLMNSTS EVLFDFLASS LQRFIEKEGN DFSLSQPLKR ELAFTFSFPV 190 200 210 220 230 240 KQTSISSGVL IKWTKGFAIS EMAGEDIAEC LQGALNKRGL DIRVAALVND TVGALSFGHF 250 260 270 280 290 300 HDPDTIAAVV FGTGSNACYL ERTDAIIKCQ NPRTTSGSMV VNMEWGNFWS SRLPRTSYDL 310 320 330 340 350 360 ELDAESMNSN DMGFEKMIGG MYLGDIVRRV ILRMSQESDI FGPISSILST PFVLRTNSVS 370 380 390 400 410 420 AMHEDDTSEL QEVARILKDL GVSEVPMKVR KLVVKICDVV TRRAARLAAA GIAGILKKVG 430 440 450 460 470 480 RDGSGGGRRS DKQIMRRTVV AVEGGLYLNY RMFREYMDEA LRDILGEDVA QHVVVKAMED 490 500 GSSIGSALLL ASSQSVQTIP SV

References

[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 18-502. Strain: cv. Columbia.
[3]	"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed: 14671022] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP000383 Genomic DNA. Translation: BAB01861.1. AK221726 mRNA. Translation: BAD93730.1. Different initiation.
IPI	IPI00523342.
RefSeq	NP_188639.2.
UniGene	At.50841
3D structure databases
HSSP	HSSP built from PDB template 1BG3 based on UniProtKB P05708.
ModBase	Search...
Protein-protein interaction databases
STRING	Q56XE8.
Genome annotation databases
GeneID	821543.
GenomeReviews	Gene locus AT3G20040 in contig BA000014_GR.
KEGG	ath:AT3G20040.
Organism-specific databases
GeneFarm	4272. 430.
TAIR	At3g20040.
Phylogenomic databases
eggNOG	KOG1369.
HOGENOM	HBG522186.
InParanoid	Q56XE8.
PhylomeDB	Q56XE8.
Enzyme and pathway databases
BRENDA	2.7.1.1. 302.
Gene expression databases
Genevestigator	Q56XE8.
Family and domain databases
InterPro	IPR001312. Hexokinase. [Graphical view]
PANTHER	PTHR19443. Hexokinase. 1 hit.
Pfam	PF00349. Hexokinase_1. 1 hit. PF03727. Hexokinase_2. 1 hit. [Graphical view]
PRINTS	PR00475. HEXOKINASE.
ProtoNet	Search...

Entry information

Entry name

HXK4_ARATH

Accession

Primary (citable) accession number: Q56XE8
Secondary accession number(s): Q9LJZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 31, 2006
Last modified:	February 9, 2010
This is version 37 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)