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Protein

Glutamine synthetase cytosolic isozyme 1-1

Gene

GLN1-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity glutamine synthetase. May contribute to the homeostatic control of glutamine synthesis in roots.

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Kineticsi

The KM value for ammonium is smaller than 10 µM. Measured at pH 7.8 and 30 degrees Celsius for all experiments.

  1. KM=1.1 mM for glutamate1 Publication
  2. KM=10 µM for ammonium1 Publication
  3. KM=300 µM for ATP1 Publication
  1. Vmax=29.3 nmol/sec/mg enzyme with glutamate as substrate1 Publication
  2. Vmax=27.4 nmol/sec/mg enzyme with ammonium as substrate1 Publication
  3. Vmax=21.4 nmol/sec/mg enzyme with ATP as substrate1 Publication

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • copper ion binding Source: TAIR
  • glutamate-ammonia ligase activity Source: TAIR

GO - Biological processi

  • glutamine biosynthetic process Source: InterPro
  • leaf senescence Source: UniProtKB
  • nitrate assimilation Source: TAIR
  • nitrogen fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.1.2. 399.
ReactomeiREACT_276185. Amino acid synthesis and interconversion (transamination).
REACT_282855. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
SABIO-RKQ56WN1.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase cytosolic isozyme 1-1 (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase GLN1;1
Short name:
GLN1;1
Gene namesi
Name:GLN1-1
Ordered Locus Names:At5g37600
ORF Names:K12B20.50
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G37600.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cell wall Source: TAIR
  • cytosol Source: TAIR
  • cytosolic ribosome Source: TAIR
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 356355Glutamine synthetase cytosolic isozyme 1-1PRO_0000239818Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei2 – 21PhosphoserineBy similarity
Modified residuei48 – 481PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by CRK3.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ56WN1.
PRIDEiQ56WN1.

Expressioni

Tissue specificityi

Expressed in root tips, root hairs and epidermis. Ubiquitously expressed with higher levels in siliques and roots.2 Publications

Inductioni

By nitrogen deprivation, sucrose, glucose and fructose. Down-regulated by ammonium supply. Induced during leaf senescence.3 Publications

Interactioni

Subunit structurei

Homooctamer (By similarity). Interacts with CRK3 and GRF3.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CRK3Q9ZUZ27EBI-1538766,EBI-1538748

Protein-protein interaction databases

BioGridi18989. 1 interaction.
IntActiQ56WN1. 2 interactions.
MINTiMINT-8062724.
STRINGi3702.AT5G37600.1.

Structurei

3D structure databases

ProteinModelPortaliQ56WN1.
SMRiQ56WN1. Positions 4-354.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiCOG0174.
HOGENOMiHOG000061500.
InParanoidiQ56WN1.
KOiK01915.
OMAiKSFGRDV.
PhylomeDBiQ56WN1.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q56WN1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLVSDLINL NLSDSTDKII AEYIWVGGSG MDMRSKARTL PGPVTDPSQL
60 70 80 90 100
PKWNYDGSST GQAPGEDSEV ILYPQAIFKD PFRRGNNILV MCDAYTPAGE
110 120 130 140 150
PIPTNKRHAA AKVFSNPDVA AEVPWYGIEQ EYTLLQKDVK WPVGWPIGGY
160 170 180 190 200
PGPQGPYYCG IGADKSFGRD VVDSHYKACL YAGINISGIN GEVMPGQWEF
210 220 230 240 250
QVGPAVGISA ADEIWVARYI LERITEIAGV VVSFDPKPIP GDWNGAGAHC
260 270 280 290 300
NYSTKSMREE GGYEIIKKAI DKLGLRHKEH IAAYGEGNER RLTGHHETAD
310 320 330 340 350
INTFLWGVAN RGASIRVGRD TEKEGKGYFE DRRPASNMDP YIVTSMIAET

TILWNP
Length:356
Mass (Da):39,115
Last modified:June 13, 2006 - v2
Checksum:iAE54FB88287CFE9D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018107 Genomic DNA. Translation: BAB08306.1.
CP002688 Genomic DNA. Translation: AED94209.1.
AF419608 mRNA. Translation: AAL31940.1.
AF428386 mRNA. Translation: AAL16154.1.
AY079113 mRNA. Translation: AAL84997.1.
BT000753 mRNA. Translation: AAN31893.1.
AK222005 mRNA. Translation: BAD94626.1.
PIRiS18601.
RefSeqiNP_198576.1. NM_123119.3.
UniGeneiAt.7003.

Genome annotation databases

EnsemblPlantsiAT5G37600.1; AT5G37600.1; AT5G37600.
GeneIDi833738.
KEGGiath:AT5G37600.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018107 Genomic DNA. Translation: BAB08306.1.
CP002688 Genomic DNA. Translation: AED94209.1.
AF419608 mRNA. Translation: AAL31940.1.
AF428386 mRNA. Translation: AAL16154.1.
AY079113 mRNA. Translation: AAL84997.1.
BT000753 mRNA. Translation: AAN31893.1.
AK222005 mRNA. Translation: BAD94626.1.
PIRiS18601.
RefSeqiNP_198576.1. NM_123119.3.
UniGeneiAt.7003.

3D structure databases

ProteinModelPortaliQ56WN1.
SMRiQ56WN1. Positions 4-354.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi18989. 1 interaction.
IntActiQ56WN1. 2 interactions.
MINTiMINT-8062724.
STRINGi3702.AT5G37600.1.

Proteomic databases

PaxDbiQ56WN1.
PRIDEiQ56WN1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G37600.1; AT5G37600.1; AT5G37600.
GeneIDi833738.
KEGGiath:AT5G37600.

Organism-specific databases

TAIRiAT5G37600.

Phylogenomic databases

eggNOGiCOG0174.
HOGENOMiHOG000061500.
InParanoidiQ56WN1.
KOiK01915.
OMAiKSFGRDV.
PhylomeDBiQ56WN1.

Enzyme and pathway databases

BRENDAi6.3.1.2. 399.
ReactomeiREACT_276185. Amino acid synthesis and interconversion (transamination).
REACT_282855. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
SABIO-RKQ56WN1.

Miscellaneous databases

PROiQ56WN1.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S.
    DNA Res. 6:183-195(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 145-356.
    Strain: cv. Columbia.
  5. "Carbon and amino acids reciprocally modulate the expression of glutamine synthetase in Arabidopsis."
    Oliveira I.C., Coruzzi G.M.
    Plant Physiol. 121:301-310(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "Kinetic properties and ammonium-dependent regulation of cytosolic isoenzymes of glutamine synthetase in Arabidopsis."
    Ishiyama K., Inoue E., Watanabe-Takahashi A., Obara M., Yamaya T., Takahashi H.
    J. Biol. Chem. 279:16598-16605(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION.
  7. "Arabidopsis cytosolic glutamine synthetase AtGLN1;1 is a potential substrate of AtCRK3 involved in leaf senescence."
    Li R.-J., Hua W., Lu Y.-T.
    Biochem. Biophys. Res. Commun. 342:119-126(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CRK3, PHOSPHORYLATION BY CRK3, INDUCTION BY LEAF SENESCENCE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  8. "14-3-3 proteins fine-tune plant nutrient metabolism."
    Shin R., Jez J.M., Basra A., Zhang B., Schachtman D.P.
    FEBS Lett. 585:143-147(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GRF3.
  9. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGLN11_ARATH
AccessioniPrimary (citable) accession number: Q56WN1
Secondary accession number(s): Q9FHR0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 13, 2006
Last modified: June 24, 2015
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.