Glutamine synthetase cytosolic isozyme 1-1 - Arabidopsis thaliana (Mouse-ear cress)

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Q56WN1 (GLN11_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 50. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Glutamine synthetase cytosolic isozyme 1-1
EC=6.3.1.2

Alternative name(s):
Glutamate--ammonia ligase GLN1;1
Short name=GLN1;1

Gene names

Name:	GLN1-1
Ordered Locus Names:	At5g37600
ORF Names:	K12B20.50

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	High-affinity glutamine synthetase. May contribute to the homeostatic control of glutamine synthesis in roots.
Catalytic activity	ATP + L-glutamate + NH₃ = ADP + phosphate + L-glutamine.
Subunit structure	Homooctamer By similarity.
Subcellular location	Cytoplasm.
Tissue specificity	Expressed in root tips, root hairs and epidermis. Ref.6
Induction	By nitrogen deprivation, sucrose, glucose and fructose. Down-regulated by ammonium supply. Ref.5 Ref.6
Sequence similarities	Belongs to the glutamine synthetase family.
Biophysicochemical properties	Kinetic parameters: The KM value for ammonium is smaller than 10 µM. Measured at pH 7.8 and 30 degrees Celsius for all experiments. K_M=1.1 mM for glutamate Ref.6 K_M=10 µM for ammonium K_M=300 µM for ATP V_max=29.3 nmol/sec/mg enzyme with glutamate as substrate V_max=27.4 nmol/sec/mg enzyme with ammonium as substrate V_max=21.4 nmol/sec/mg enzyme with ATP as substrate

Ontologies

Keywords
Biological process	Nitrogen fixation
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glutamine biosynthetic process Inferred from electronic annotation. Source: InterPro nitrate assimilation Traceable author statement. Source: TAIR nitrogen fixation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from direct assay. Source: TAIR cytosolic ribosome Inferred from direct assay. Source: TAIR plasma membrane Inferred from direct assay. Source: TAIR plasmodesma Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW copper ion binding Inferred from direct assay. Source: TAIR glutamate-ammonia ligase activity Inferred from direct assay Ref.6. Source: TAIR protein binding Inferred from physical interaction Ref.7. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CRK3	Q9ZUZ2	7	EBI-1538766,EBI-1538748

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 356

356

Glutamine synthetase cytosolic isozyme 1-1

PRO_0000239818

Sequences

Sequence

Length

Mass (Da)

Tools

Q56WN1 [UniParc].

Last modified June 13, 2006. Version 2.
Checksum: AE54FB88287CFE9D
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FASTA

356

39,115

        10         20         30         40         50         60 
MSLVSDLINL NLSDSTDKII AEYIWVGGSG MDMRSKARTL PGPVTDPSQL PKWNYDGSST 

        70         80         90        100        110        120 
GQAPGEDSEV ILYPQAIFKD PFRRGNNILV MCDAYTPAGE PIPTNKRHAA AKVFSNPDVA 

       130        140        150        160        170        180 
AEVPWYGIEQ EYTLLQKDVK WPVGWPIGGY PGPQGPYYCG IGADKSFGRD VVDSHYKACL 

       190        200        210        220        230        240 
YAGINISGIN GEVMPGQWEF QVGPAVGISA ADEIWVARYI LERITEIAGV VVSFDPKPIP 

       250        260        270        280        290        300 
GDWNGAGAHC NYSTKSMREE GGYEIIKKAI DKLGLRHKEH IAAYGEGNER RLTGHHETAD 

       310        320        330        340        350 
INTFLWGVAN RGASIRVGRD TEKEGKGYFE DRRPASNMDP YIVTSMIAET TILWNP

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S. DNA Res. 6:183-195(1999) [PubMed: 10470850] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 145-356. Strain: cv. Columbia.
[5]	"Carbon and amino acids reciprocally modulate the expression of glutamine synthetase in Arabidopsis." Oliveira I.C., Coruzzi G.M. Plant Physiol. 121:301-310(1999) [PubMed: 10482686] [Abstract] Cited for: INDUCTION.
[6]	"Kinetic properties and ammonium-dependent regulation of cytosolic isoenzymes of glutamine synthetase in Arabidopsis." Ishiyama K., Inoue E., Watanabe-Takahashi A., Obara M., Yamaya T., Takahashi H. J. Biol. Chem. 279:16598-16605(2004) [PubMed: 14757761] [Abstract] Cited for: BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION.
[7]	"Arabidopsis cytosolic glutamine synthetase AtGLN1;1 is a potential substrate of AtCRK3 involved in leaf senescence." Li R.-J., Hua W., Lu Y.-T. Biochem. Biophys. Res. Commun. 342:119-126(2006) [PubMed: 16472779] [Abstract] Cited for: INTERACTION WITH CRK3/CAMK4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB018107 Genomic DNA. Translation: BAB08306.1. CP002688 Genomic DNA. Translation: AED94209.1. AF419608 mRNA. Translation: AAL31940.1. AF428386 mRNA. Translation: AAL16154.1. AY079113 mRNA. Translation: AAL84997.1. BT000753 mRNA. Translation: AAN31893.1. AK222005 mRNA. Translation: BAD94626.1.
IPI	IPI00519748.
PIR	S18601.
RefSeq	NP_198576.1. NM_123119.3.
UniGene	At.7003.
3D structure databases
ProteinModelPortal	Q56WN1.
SMR	Q56WN1. Positions 4-354.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q56WN1. 2 interactions.
STRING	Q56WN1.
Proteomic databases
PRIDE	Q56WN1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G37600.1; AT5G37600.1; AT5G37600.
GeneID	833738.
GenomeReviews	Gene locus AT5G37600 in contig BA000015_GR.
KEGG	ath:AT5G37600.
NMPDR	fig\|3702.1.peg.25447.
Organism-specific databases
TAIR	At5g37600.
Phylogenomic databases
eggNOG	KOG0683.
GeneTree	EPGT00050000002349.
HOGENOM	HBG299709.
InParanoid	Q56WN1.
OMA	DPYRVTS.
PhylomeDB	Q56WN1.
ProtClustDB	PLN02284.
Enzyme and pathway databases
BRENDA	6.3.1.2. 399.
Gene expression databases
Genevestigator	Q56WN1.
GermOnline	AT5G37600. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR008147. Gln_synt_beta. IPR014746. Gln_synth/guanido_kin_cat_dom. IPR008146. Gln_synth_cat_dom. [Graphical view]
Gene3D	G3DSA:3.30.590.10. ATP-gua_Ptrans. 1 hit. G3DSA:3.10.20.70. G3DSA:3.10.20.70. 1 hit.
KO	K01915.
Pfam	PF00120. Gln-synt_C. 1 hit. PF03951. Gln-synt_N. 1 hit. [Graphical view]
SUPFAM	SSF54368. Gln_synt_beta. 1 hit.
PROSITE	PS00180. GLNA_1. 1 hit. PS00181. GLNA_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLN11_ARATH

Accession

Primary (citable) accession number: Q56WN1
Secondary accession number(s): Q9FHR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	June 13, 2006
Last modified:	December 14, 2011
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents