Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q56WN1 (GLN11_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase cytosolic isozyme 1-1

EC=6.3.1.2
Alternative name(s):
Glutamate--ammonia ligase GLN1;1
Short name=GLN1;1
Gene names
Name:GLN1-1
Ordered Locus Names:At5g37600
ORF Names:K12B20.50
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

High-affinity glutamine synthetase. May contribute to the homeostatic control of glutamine synthesis in roots.

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Homooctamer By similarity. Interacts with CRK3. Ref.7

Subcellular location

Cytoplasm Ref.7.

Tissue specificity

Expressed in root tips, root hairs and epidermis. Ubiquitously expressed with higher levels in siliques and roots. Ref.6 Ref.7

Induction

By nitrogen deprivation, sucrose, glucose and fructose. Down-regulated by ammonium supply. Induced during leaf senescence. Ref.5 Ref.6 Ref.7

Post-translational modification

Phosphorylated by CRK3. Ref.7

Sequence similarities

Belongs to the glutamine synthetase family.

Biophysicochemical properties

Kinetic parameters:

The KM value for ammonium is smaller than 10 µM. Measured at pH 7.8 and 30 degrees Celsius for all experiments.

KM=1.1 mM for glutamate Ref.6

KM=10 µM for ammonium

KM=300 µM for ATP

Vmax=29.3 nmol/sec/mg enzyme with glutamate as substrate

Vmax=27.4 nmol/sec/mg enzyme with ammonium as substrate

Vmax=21.4 nmol/sec/mg enzyme with ATP as substrate

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CRK3Q9ZUZ27EBI-1538766,EBI-1538748

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.8
Chain2 – 356355Glutamine synthetase cytosolic isozyme 1-1
PRO_0000239818

Amino acid modifications

Modified residue21N-acetylserine Ref.8
Modified residue21Phosphoserine By similarity
Modified residue481Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q56WN1 [UniParc].

Last modified June 13, 2006. Version 2.
Checksum: AE54FB88287CFE9D

FASTA35639,115
        10         20         30         40         50         60 
MSLVSDLINL NLSDSTDKII AEYIWVGGSG MDMRSKARTL PGPVTDPSQL PKWNYDGSST 

        70         80         90        100        110        120 
GQAPGEDSEV ILYPQAIFKD PFRRGNNILV MCDAYTPAGE PIPTNKRHAA AKVFSNPDVA 

       130        140        150        160        170        180 
AEVPWYGIEQ EYTLLQKDVK WPVGWPIGGY PGPQGPYYCG IGADKSFGRD VVDSHYKACL 

       190        200        210        220        230        240 
YAGINISGIN GEVMPGQWEF QVGPAVGISA ADEIWVARYI LERITEIAGV VVSFDPKPIP 

       250        260        270        280        290        300 
GDWNGAGAHC NYSTKSMREE GGYEIIKKAI DKLGLRHKEH IAAYGEGNER RLTGHHETAD 

       310        320        330        340        350 
INTFLWGVAN RGASIRVGRD TEKEGKGYFE DRRPASNMDP YIVTSMIAET TILWNP 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S.
DNA Res. 6:183-195(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 145-356.
Strain: cv. Columbia.
[5]"Carbon and amino acids reciprocally modulate the expression of glutamine synthetase in Arabidopsis."
Oliveira I.C., Coruzzi G.M.
Plant Physiol. 121:301-310(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[6]"Kinetic properties and ammonium-dependent regulation of cytosolic isoenzymes of glutamine synthetase in Arabidopsis."
Ishiyama K., Inoue E., Watanabe-Takahashi A., Obara M., Yamaya T., Takahashi H.
J. Biol. Chem. 279:16598-16605(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION.
[7]"Arabidopsis cytosolic glutamine synthetase AtGLN1;1 is a potential substrate of AtCRK3 involved in leaf senescence."
Li R.-J., Hua W., Lu Y.-T.
Biochem. Biophys. Res. Commun. 342:119-126(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CRK3, PHOSPHORYLATION BY CRK3, INDUCTION BY LEAF SENESCENCE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[8]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB018107 Genomic DNA. Translation: BAB08306.1.
CP002688 Genomic DNA. Translation: AED94209.1.
AF419608 mRNA. Translation: AAL31940.1.
AF428386 mRNA. Translation: AAL16154.1.
AY079113 mRNA. Translation: AAL84997.1.
BT000753 mRNA. Translation: AAN31893.1.
AK222005 mRNA. Translation: BAD94626.1.
PIRS18601.
RefSeqNP_198576.1. NM_123119.3.
UniGeneAt.7003.

3D structure databases

ProteinModelPortalQ56WN1.
SMRQ56WN1. Positions 4-354.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid18989. 1 interaction.
IntActQ56WN1. 2 interactions.
MINTMINT-8062724.
STRING3702.AT5G37600.1-P.

Proteomic databases

PaxDbQ56WN1.
PRIDEQ56WN1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G37600.1; AT5G37600.1; AT5G37600.
GeneID833738.
KEGGath:AT5G37600.

Organism-specific databases

TAIRAT5G37600.

Phylogenomic databases

eggNOGCOG0174.
HOGENOMHOG000061500.
InParanoidQ56WN1.
KOK01915.
OMAKSFGRDV.
PhylomeDBQ56WN1.

Enzyme and pathway databases

BRENDA6.3.1.2. 399.
SABIO-RKQ56WN1.

Gene expression databases

GenevestigatorQ56WN1.

Family and domain databases

Gene3D3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLN11_ARATH
AccessionPrimary (citable) accession number: Q56WN1
Secondary accession number(s): Q9FHR0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 13, 2006
Last modified: June 11, 2014
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names