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Protein

Glutamine synthetase cytosolic isozyme 1-1

Gene

GLN1-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity glutamine synthetase. May contribute to the homeostatic control of glutamine synthesis in roots.

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Kineticsi

The KM value for ammonium is smaller than 10 µM. Measured at pH 7.8 and 30 degrees Celsius for all experiments.

  1. KM=1.1 mM for glutamate1 Publication
  2. KM=10 µM for ammonium1 Publication
  3. KM=300 µM for ATP1 Publication
  1. Vmax=29.3 nmol/sec/mg enzyme with glutamate as substrate1 Publication
  2. Vmax=27.4 nmol/sec/mg enzyme with ammonium as substrate1 Publication
  3. Vmax=21.4 nmol/sec/mg enzyme with ATP as substrate1 Publication

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • copper ion binding Source: TAIR
  • glutamate-ammonia ligase activity Source: TAIR

GO - Biological processi

  • glutamine biosynthetic process Source: InterPro
  • leaf senescence Source: UniProtKB
  • nitrate assimilation Source: TAIR
  • nitrogen fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.1.2. 399.
ReactomeiR-ATH-210455. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
R-ATH-70614. Amino acid synthesis and interconversion (transamination).
SABIO-RKQ56WN1.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase cytosolic isozyme 1-1 (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase GLN1;1
Short name:
GLN1;1
Gene namesi
Name:GLN1-1
Ordered Locus Names:At5g37600
ORF Names:K12B20.50
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G37600.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cell wall Source: TAIR
  • cytosol Source: TAIR
  • cytosolic ribosome Source: TAIR
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002398182 – 356Glutamine synthetase cytosolic isozyme 1-1Add BLAST355

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei2PhosphoserineBy similarity1
Modified residuei48PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by CRK3.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ56WN1.
PRIDEiQ56WN1.

PTM databases

iPTMnetiQ56WN1.

Expressioni

Tissue specificityi

Expressed in root tips, root hairs and epidermis. Ubiquitously expressed with higher levels in siliques and roots.2 Publications

Inductioni

By nitrogen deprivation, sucrose, glucose and fructose. Down-regulated by ammonium supply. Induced during leaf senescence.3 Publications

Gene expression databases

GenevisibleiQ56WN1. AT.

Interactioni

Subunit structurei

Homooctamer (By similarity). Interacts with CRK3 and GRF3.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CRK3Q9ZUZ27EBI-1538766,EBI-1538748

Protein-protein interaction databases

BioGridi18989. 4 interactors.
IntActiQ56WN1. 2 interactors.
MINTiMINT-8062724.
STRINGi3702.AT5G37600.1.

Structurei

3D structure databases

ProteinModelPortaliQ56WN1.
SMRiQ56WN1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiKOG0683. Eukaryota.
COG0174. LUCA.
HOGENOMiHOG000061500.
InParanoidiQ56WN1.
KOiK01915.
OMAiGIMCETV.
OrthoDBiEOG09360CB0.
PhylomeDBiQ56WN1.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SMARTiSM01230. Gln-synt_C. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q56WN1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLVSDLINL NLSDSTDKII AEYIWVGGSG MDMRSKARTL PGPVTDPSQL
60 70 80 90 100
PKWNYDGSST GQAPGEDSEV ILYPQAIFKD PFRRGNNILV MCDAYTPAGE
110 120 130 140 150
PIPTNKRHAA AKVFSNPDVA AEVPWYGIEQ EYTLLQKDVK WPVGWPIGGY
160 170 180 190 200
PGPQGPYYCG IGADKSFGRD VVDSHYKACL YAGINISGIN GEVMPGQWEF
210 220 230 240 250
QVGPAVGISA ADEIWVARYI LERITEIAGV VVSFDPKPIP GDWNGAGAHC
260 270 280 290 300
NYSTKSMREE GGYEIIKKAI DKLGLRHKEH IAAYGEGNER RLTGHHETAD
310 320 330 340 350
INTFLWGVAN RGASIRVGRD TEKEGKGYFE DRRPASNMDP YIVTSMIAET

TILWNP
Length:356
Mass (Da):39,115
Last modified:June 13, 2006 - v2
Checksum:iAE54FB88287CFE9D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018107 Genomic DNA. Translation: BAB08306.1.
CP002688 Genomic DNA. Translation: AED94209.1.
AF419608 mRNA. Translation: AAL31940.1.
AF428386 mRNA. Translation: AAL16154.1.
AY079113 mRNA. Translation: AAL84997.1.
BT000753 mRNA. Translation: AAN31893.1.
AK222005 mRNA. Translation: BAD94626.1.
PIRiS18601.
RefSeqiNP_198576.1. NM_123119.4.
UniGeneiAt.7003.

Genome annotation databases

EnsemblPlantsiAT5G37600.1; AT5G37600.1; AT5G37600.
GeneIDi833738.
GrameneiAT5G37600.1; AT5G37600.1; AT5G37600.
KEGGiath:AT5G37600.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018107 Genomic DNA. Translation: BAB08306.1.
CP002688 Genomic DNA. Translation: AED94209.1.
AF419608 mRNA. Translation: AAL31940.1.
AF428386 mRNA. Translation: AAL16154.1.
AY079113 mRNA. Translation: AAL84997.1.
BT000753 mRNA. Translation: AAN31893.1.
AK222005 mRNA. Translation: BAD94626.1.
PIRiS18601.
RefSeqiNP_198576.1. NM_123119.4.
UniGeneiAt.7003.

3D structure databases

ProteinModelPortaliQ56WN1.
SMRiQ56WN1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi18989. 4 interactors.
IntActiQ56WN1. 2 interactors.
MINTiMINT-8062724.
STRINGi3702.AT5G37600.1.

PTM databases

iPTMnetiQ56WN1.

Proteomic databases

PaxDbiQ56WN1.
PRIDEiQ56WN1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G37600.1; AT5G37600.1; AT5G37600.
GeneIDi833738.
GrameneiAT5G37600.1; AT5G37600.1; AT5G37600.
KEGGiath:AT5G37600.

Organism-specific databases

TAIRiAT5G37600.

Phylogenomic databases

eggNOGiKOG0683. Eukaryota.
COG0174. LUCA.
HOGENOMiHOG000061500.
InParanoidiQ56WN1.
KOiK01915.
OMAiGIMCETV.
OrthoDBiEOG09360CB0.
PhylomeDBiQ56WN1.

Enzyme and pathway databases

BRENDAi6.3.1.2. 399.
ReactomeiR-ATH-210455. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
R-ATH-70614. Amino acid synthesis and interconversion (transamination).
SABIO-RKQ56WN1.

Miscellaneous databases

PROiQ56WN1.

Gene expression databases

GenevisibleiQ56WN1. AT.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SMARTiSM01230. Gln-synt_C. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLN11_ARATH
AccessioniPrimary (citable) accession number: Q56WN1
Secondary accession number(s): Q9FHR0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 13, 2006
Last modified: November 30, 2016
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.