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Q56WN1

- GLN11_ARATH

UniProt

Q56WN1 - GLN11_ARATH

Protein

Glutamine synthetase cytosolic isozyme 1-1

Gene

GLN1-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 2 (13 Jun 2006)
      Previous versions | rss
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    Functioni

    High-affinity glutamine synthetase. May contribute to the homeostatic control of glutamine synthesis in roots.

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    Kineticsi

    The KM value for ammonium is smaller than 10 µM. Measured at pH 7.8 and 30 degrees Celsius for all experiments.

    1. KM=1.1 mM for glutamate1 Publication
    2. KM=10 µM for ammonium1 Publication
    3. KM=300 µM for ATP1 Publication

    Vmax=29.3 nmol/sec/mg enzyme with glutamate as substrate1 Publication

    Vmax=27.4 nmol/sec/mg enzyme with ammonium as substrate1 Publication

    Vmax=21.4 nmol/sec/mg enzyme with ATP as substrate1 Publication

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. copper ion binding Source: TAIR
    3. glutamate-ammonia ligase activity Source: TAIR
    4. protein binding Source: IntAct

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro
    2. leaf senescence Source: UniProtKB
    3. nitrate assimilation Source: TAIR
    4. nitrogen fixation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Nitrogen fixation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi6.3.1.2. 399.
    SABIO-RKQ56WN1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase cytosolic isozyme 1-1 (EC:6.3.1.2)
    Alternative name(s):
    Glutamate--ammonia ligase GLN1;1
    Short name:
    GLN1;1
    Gene namesi
    Name:GLN1-1
    Ordered Locus Names:At5g37600
    ORF Names:K12B20.50
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G37600.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cell wall Source: TAIR
    2. cytosol Source: TAIR
    3. cytosolic ribosome Source: TAIR
    4. plasma membrane Source: TAIR
    5. plasmodesma Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 356355Glutamine synthetase cytosolic isozyme 1-1PRO_0000239818Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei2 – 21PhosphoserineBy similarity
    Modified residuei48 – 481PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated by CRK3.1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ56WN1.
    PRIDEiQ56WN1.

    Expressioni

    Tissue specificityi

    Expressed in root tips, root hairs and epidermis. Ubiquitously expressed with higher levels in siliques and roots.2 Publications

    Inductioni

    By nitrogen deprivation, sucrose, glucose and fructose. Down-regulated by ammonium supply. Induced during leaf senescence.3 Publications

    Gene expression databases

    GenevestigatoriQ56WN1.

    Interactioni

    Subunit structurei

    Homooctamer By similarity. Interacts with CRK3.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CRK3Q9ZUZ27EBI-1538766,EBI-1538748

    Protein-protein interaction databases

    BioGridi18989. 1 interaction.
    IntActiQ56WN1. 2 interactions.
    MINTiMINT-8062724.
    STRINGi3702.AT5G37600.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ56WN1.
    SMRiQ56WN1. Positions 4-354.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Phylogenomic databases

    eggNOGiCOG0174.
    HOGENOMiHOG000061500.
    InParanoidiQ56WN1.
    KOiK01915.
    OMAiKSFGRDV.
    PhylomeDBiQ56WN1.

    Family and domain databases

    Gene3Di3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q56WN1-1 [UniParc]FASTAAdd to Basket

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    MSLVSDLINL NLSDSTDKII AEYIWVGGSG MDMRSKARTL PGPVTDPSQL    50
    PKWNYDGSST GQAPGEDSEV ILYPQAIFKD PFRRGNNILV MCDAYTPAGE 100
    PIPTNKRHAA AKVFSNPDVA AEVPWYGIEQ EYTLLQKDVK WPVGWPIGGY 150
    PGPQGPYYCG IGADKSFGRD VVDSHYKACL YAGINISGIN GEVMPGQWEF 200
    QVGPAVGISA ADEIWVARYI LERITEIAGV VVSFDPKPIP GDWNGAGAHC 250
    NYSTKSMREE GGYEIIKKAI DKLGLRHKEH IAAYGEGNER RLTGHHETAD 300
    INTFLWGVAN RGASIRVGRD TEKEGKGYFE DRRPASNMDP YIVTSMIAET 350
    TILWNP 356
    Length:356
    Mass (Da):39,115
    Last modified:June 13, 2006 - v2
    Checksum:iAE54FB88287CFE9D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB018107 Genomic DNA. Translation: BAB08306.1.
    CP002688 Genomic DNA. Translation: AED94209.1.
    AF419608 mRNA. Translation: AAL31940.1.
    AF428386 mRNA. Translation: AAL16154.1.
    AY079113 mRNA. Translation: AAL84997.1.
    BT000753 mRNA. Translation: AAN31893.1.
    AK222005 mRNA. Translation: BAD94626.1.
    PIRiS18601.
    RefSeqiNP_198576.1. NM_123119.3.
    UniGeneiAt.7003.

    Genome annotation databases

    EnsemblPlantsiAT5G37600.1; AT5G37600.1; AT5G37600.
    GeneIDi833738.
    KEGGiath:AT5G37600.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB018107 Genomic DNA. Translation: BAB08306.1 .
    CP002688 Genomic DNA. Translation: AED94209.1 .
    AF419608 mRNA. Translation: AAL31940.1 .
    AF428386 mRNA. Translation: AAL16154.1 .
    AY079113 mRNA. Translation: AAL84997.1 .
    BT000753 mRNA. Translation: AAN31893.1 .
    AK222005 mRNA. Translation: BAD94626.1 .
    PIRi S18601.
    RefSeqi NP_198576.1. NM_123119.3.
    UniGenei At.7003.

    3D structure databases

    ProteinModelPortali Q56WN1.
    SMRi Q56WN1. Positions 4-354.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 18989. 1 interaction.
    IntActi Q56WN1. 2 interactions.
    MINTi MINT-8062724.
    STRINGi 3702.AT5G37600.1-P.

    Proteomic databases

    PaxDbi Q56WN1.
    PRIDEi Q56WN1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G37600.1 ; AT5G37600.1 ; AT5G37600 .
    GeneIDi 833738.
    KEGGi ath:AT5G37600.

    Organism-specific databases

    TAIRi AT5G37600.

    Phylogenomic databases

    eggNOGi COG0174.
    HOGENOMi HOG000061500.
    InParanoidi Q56WN1.
    KOi K01915.
    OMAi KSFGRDV.
    PhylomeDBi Q56WN1.

    Enzyme and pathway databases

    BRENDAi 6.3.1.2. 399.
    SABIO-RK Q56WN1.

    Gene expression databases

    Genevestigatori Q56WN1.

    Family and domain databases

    Gene3Di 3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence features of the regions of 1,011,550 bp covered by seventeen P1 and TAC clones."
      Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H., Miyajima N., Tabata S.
      DNA Res. 6:183-195(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 145-356.
      Strain: cv. Columbia.
    5. "Carbon and amino acids reciprocally modulate the expression of glutamine synthetase in Arabidopsis."
      Oliveira I.C., Coruzzi G.M.
      Plant Physiol. 121:301-310(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    6. "Kinetic properties and ammonium-dependent regulation of cytosolic isoenzymes of glutamine synthetase in Arabidopsis."
      Ishiyama K., Inoue E., Watanabe-Takahashi A., Obara M., Yamaya T., Takahashi H.
      J. Biol. Chem. 279:16598-16605(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION.
    7. "Arabidopsis cytosolic glutamine synthetase AtGLN1;1 is a potential substrate of AtCRK3 involved in leaf senescence."
      Li R.-J., Hua W., Lu Y.-T.
      Biochem. Biophys. Res. Commun. 342:119-126(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CRK3, PHOSPHORYLATION BY CRK3, INDUCTION BY LEAF SENESCENCE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    8. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGLN11_ARATH
    AccessioniPrimary (citable) accession number: Q56WN1
    Secondary accession number(s): Q9FHR0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 13, 2006
    Last sequence update: June 13, 2006
    Last modified: October 1, 2014
    This is version 71 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3