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Protein

Glutamine synthetase cytosolic isozyme 1-1

Gene

GLN1-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

High-affinity glutamine synthetase. May contribute to the homeostatic control of glutamine synthesis in roots.

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Kineticsi

The KM value for ammonium is smaller than 10 µM. Measured at pH 7.8 and 30 degrees Celsius for all experiments.
  1. KM=1.1 mM for glutamate1 Publication
  2. KM=10 µM for ammonium1 Publication
  3. KM=300 µM for ATP1 Publication
  1. Vmax=29.3 nmol/sec/mg enzyme with glutamate as substrate1 Publication
  2. Vmax=27.4 nmol/sec/mg enzyme with ammonium as substrate1 Publication
  3. Vmax=21.4 nmol/sec/mg enzyme with ATP as substrate1 Publication

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • copper ion binding Source: TAIR
  • glutamate-ammonia ligase activity Source: TAIR

GO - Biological processi

  • glutamine biosynthetic process Source: InterPro
  • leaf senescence Source: UniProtKB
  • nitrate assimilation Source: TAIR

Keywordsi

Molecular functionLigase
Biological processNitrogen fixation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.1.2 399
ReactomeiR-ATH-210455 Astrocytic Glutamate-Glutamine Uptake And Metabolism
R-ATH-70614 Amino acid synthesis and interconversion (transamination)
SABIO-RKiQ56WN1

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase cytosolic isozyme 1-1 (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase GLN1;1
Short name:
GLN1;1
Gene namesi
Name:GLN1-1
Ordered Locus Names:At5g37600
ORF Names:K12B20.50
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi

Organism-specific databases

AraportiAT5G37600
TAIRilocus:2151739 AT5G37600

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002398182 – 356Glutamine synthetase cytosolic isozyme 1-1Add BLAST355

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei2PhosphoserineBy similarity1
Modified residuei48PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by CRK3.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ56WN1
PRIDEiQ56WN1

PTM databases

iPTMnetiQ56WN1

Expressioni

Tissue specificityi

Expressed in root tips, root hairs and epidermis. Ubiquitously expressed with higher levels in siliques and roots.2 Publications

Inductioni

By nitrogen deprivation, sucrose, glucose and fructose. Down-regulated by ammonium supply. Induced during leaf senescence.3 Publications

Gene expression databases

ExpressionAtlasiQ56WN1 baseline and differential
GenevisibleiQ56WN1 AT

Interactioni

Subunit structurei

Homooctamer (By similarity). Interacts with CRK3 and GRF3.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CRK3Q9ZUZ27EBI-1538766,EBI-1538748

Protein-protein interaction databases

BioGridi18989, 4 interactors
IntActiQ56WN1, 2 interactors
STRINGi3702.AT5G37600.1

Structurei

3D structure databases

ProteinModelPortaliQ56WN1
SMRiQ56WN1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiKOG0683 Eukaryota
COG0174 LUCA
HOGENOMiHOG000061500
InParanoidiQ56WN1
KOiK01915
OMAiCETLTHD
OrthoDBiEOG09360CB0
PhylomeDBiQ56WN1

Family and domain databases

Gene3Di3.10.20.70, 1 hit
InterProiView protein in InterPro
IPR008147 Gln_synt_b-grasp
IPR036651 Gln_synt_N
IPR014746 Gln_synth/guanido_kin_cat_dom
IPR008146 Gln_synth_cat_dom
IPR027303 Gln_synth_gly_rich_site
IPR027302 Gln_synth_N_conserv_site
PfamiView protein in Pfam
PF00120 Gln-synt_C, 1 hit
PF03951 Gln-synt_N, 1 hit
SMARTiView protein in SMART
SM01230 Gln-synt_C, 1 hit
SUPFAMiSSF54368 SSF54368, 1 hit
SSF55931 SSF55931, 1 hit
PROSITEiView protein in PROSITE
PS00180 GLNA_1, 1 hit
PS00181 GLNA_ATP, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q56WN1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLVSDLINL NLSDSTDKII AEYIWVGGSG MDMRSKARTL PGPVTDPSQL
60 70 80 90 100
PKWNYDGSST GQAPGEDSEV ILYPQAIFKD PFRRGNNILV MCDAYTPAGE
110 120 130 140 150
PIPTNKRHAA AKVFSNPDVA AEVPWYGIEQ EYTLLQKDVK WPVGWPIGGY
160 170 180 190 200
PGPQGPYYCG IGADKSFGRD VVDSHYKACL YAGINISGIN GEVMPGQWEF
210 220 230 240 250
QVGPAVGISA ADEIWVARYI LERITEIAGV VVSFDPKPIP GDWNGAGAHC
260 270 280 290 300
NYSTKSMREE GGYEIIKKAI DKLGLRHKEH IAAYGEGNER RLTGHHETAD
310 320 330 340 350
INTFLWGVAN RGASIRVGRD TEKEGKGYFE DRRPASNMDP YIVTSMIAET

TILWNP
Length:356
Mass (Da):39,115
Last modified:June 13, 2006 - v2
Checksum:iAE54FB88287CFE9D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018107 Genomic DNA Translation: BAB08306.1
CP002688 Genomic DNA Translation: AED94209.1
AF419608 mRNA Translation: AAL31940.1
AF428386 mRNA Translation: AAL16154.1
AY079113 mRNA Translation: AAL84997.1
BT000753 mRNA Translation: AAN31893.1
AK222005 mRNA Translation: BAD94626.1
PIRiS18601
RefSeqiNP_198576.1, NM_123119.4
UniGeneiAt.7003

Genome annotation databases

EnsemblPlantsiAT5G37600.1; AT5G37600.1; AT5G37600
GeneIDi833738
GrameneiAT5G37600.1; AT5G37600.1; AT5G37600
KEGGiath:AT5G37600

Similar proteinsi

Entry informationi

Entry nameiGLN11_ARATH
AccessioniPrimary (citable) accession number: Q56WN1
Secondary accession number(s): Q9FHR0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 13, 2006
Last modified: April 25, 2018
This is version 96 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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