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Protein

Probable isoaspartyl peptidase/L-asparaginase 3

Gene

At5g61540

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Acts in asparagine catabolism but also in the final steps of protein degradation via hydrolysis of a range of isoaspartyl dipeptides.By similarity

Catalytic activityi

Cleavage of a beta-linked Asp residue from the N-terminus of a polypeptide.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei224NucleophileBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, Protease

Enzyme and pathway databases

BioCyciARA:AT5G61540-MONOMER.
ReactomeiR-ATH-6798695. Neutrophil degranulation.

Protein family/group databases

MEROPSiT02.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable isoaspartyl peptidase/L-asparaginase 3 (EC:3.4.19.5)
Alternative name(s):
L-asparagine amidohydrolase 3
Cleaved into the following 2 chains:
Gene namesi
Ordered Locus Names:At5g61540
ORF Names:K11J9.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G61540.
TAIRilocus:2151626. AT5G61540.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000454461 – 223Isoaspartyl peptidase/L-asparaginase 3 subunit alphaAdd BLAST223
ChainiPRO_0000045447224 – 359Isoaspartyl peptidase/L-asparaginase 3 subunit betaAdd BLAST136

Post-translational modificationi

Cleaved into an alpha and beta chain by autocatalysis; this activates the enzyme. The N-terminal residue of the beta subunit is responsible for the nucleophile hydrolase activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei223 – 224Cleavage; by autolysisBy similarity2

Keywords - PTMi

Autocatalytic cleavage

Proteomic databases

PaxDbiQ56W64.
PRIDEiQ56W64.

Expressioni

Gene expression databases

ExpressionAtlasiQ56W64. baseline and differential.
GenevisibleiQ56W64. AT.

Interactioni

Subunit structurei

Heterotetramer of two alpha and two beta chains arranged as a dimer of alpha/beta heterodimers.By similarity

Protein-protein interaction databases

STRINGi3702.AT5G61540.1.

Structurei

3D structure databases

ProteinModelPortaliQ56W64.
SMRiQ56W64.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni252 – 255Substrate bindingBy similarity4
Regioni275 – 278Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the Ntn-hydrolase family.Curated

Phylogenomic databases

eggNOGiKOG1593. Eukaryota.
COG1446. LUCA.
HOGENOMiHOG000174614.
InParanoidiQ56W64.
KOiK01444.
OMAiNIENHDT.
OrthoDBiEOG09360DCI.
PhylomeDBiQ56W64.

Family and domain databases

InterProiView protein in InterPro
IPR029055. Ntn_hydrolases_N.
IPR000246. Peptidase_T2.
PANTHERiPTHR10188. PTHR10188. 1 hit.
PfamiView protein in Pfam
PF01112. Asparaginase_2. 1 hit.
SUPFAMiSSF56235. SSF56235. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q56W64-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARSDVLIFV STLLLFLSLL TVADAELVKS DKFPVVVSTW PFLEAVRAAW
60 70 80 90 100
RAVDNGSSAV EAVVEGCSAC EELRCDGTVG PGGSPDENGE TMIDALVMDG
110 120 130 140 150
VTMEVGAVAA MRYVKDGIRA AHLVMKYSQH TLLAGEGASA FAISMGLPGP
160 170 180 190 200
MNLSSPESVK KWSDWKENQC QPNFRKNVVP ANDCGPYKPN NSAMNVFVDK
210 220 230 240 250
STESCEMGAI EYKPPLVGPH NHDTISMAVI DRMGHIAVGT STNGATYKIP
260 270 280 290 300
GRVGDGPIVG SSAYADDEVG GCGATGDGDT MMRFLPCYQV VESMRQGMKP
310 320 330 340 350
EEAAKDAISR IARKFPDFVG AVVAVDKNGS HAGACYGWTF QYSVQNPDMN

DVQVFTVLP
Length:359
Mass (Da):38,249
Last modified:May 10, 2005 - v1
Checksum:i6E8829A1318D82D8
GO
Isoform 2 (identifier: Q56W64-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-102: Missing.

Note: May be due to intron retention or to a competing acceptor splice site.
Show »
Length:257
Mass (Da):27,547
Checksum:i84F9ED6E444A9A21
GO

Sequence cautioni

The sequence BAB08998 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0169371 – 102Missing in isoform 2. 2 PublicationsAdd BLAST102

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012239 Genomic DNA. Translation: BAB08998.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED97483.1.
CP002688 Genomic DNA. Translation: AED97484.1.
CP002688 Genomic DNA. Translation: AED97485.1.
BT020388 mRNA. Translation: AAV91334.1.
BT022060 mRNA. Translation: AAY25472.1.
AK222183 mRNA. Translation: BAD95316.1.
AK230301 mRNA. Translation: BAF02102.1.
RefSeqiNP_001032119.1. NM_001037042.1. [Q56W64-2]
NP_200962.2. NM_125547.4. [Q56W64-1]
NP_974974.1. NM_203245.2. [Q56W64-2]
UniGeneiAt.50546.

Genome annotation databases

EnsemblPlantsiAT5G61540.1; AT5G61540.1; AT5G61540. [Q56W64-1]
AT5G61540.2; AT5G61540.2; AT5G61540. [Q56W64-2]
AT5G61540.3; AT5G61540.3; AT5G61540. [Q56W64-2]
GeneIDi836275.
GrameneiAT5G61540.1; AT5G61540.1; AT5G61540.
AT5G61540.2; AT5G61540.2; AT5G61540.
AT5G61540.3; AT5G61540.3; AT5G61540.
KEGGiath:AT5G61540.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiASPG3_ARATH
AccessioniPrimary (citable) accession number: Q56W64
Secondary accession number(s): Q27GI5, Q5M760, Q9FKG7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: May 10, 2005
Last modified: August 30, 2017
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families