Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q56W08

- PLCD3_ARATH

UniProt

Q56W08 - PLCD3_ARATH

Protein

Phosphoinositide phospholipase C 3

Gene

PLC3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (10 May 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.1 Publication

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

    Cofactori

    Calcium.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei121 – 1211PROSITE-ProRule annotation
    Active sitei167 – 1671PROSITE-ProRule annotation

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
    3. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. lipid catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Transducer

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Enzyme and pathway databases

    BioCyciARA:AT4G38530-MONOMER.
    ReactomeiREACT_187614. Synthesis of IP3 and IP4 in the cytosol.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoinositide phospholipase C 3 (EC:3.1.4.11)
    Alternative name(s):
    Phosphoinositide phospholipase PLC3
    Short name:
    AtPLC1F
    Short name:
    AtPLC3
    Short name:
    PI-PLC3
    Gene namesi
    Name:PLC3
    Synonyms:ATPLC1
    Ordered Locus Names:At4g38530
    ORF Names:F20M13.90
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G38530.

    Subcellular locationi

    Cell membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 564564Phosphoinositide phospholipase C 3PRO_0000324128Add
    BLAST

    Proteomic databases

    PRIDEiQ56W08.

    Expressioni

    Tissue specificityi

    Expressed in leaves, roots and siliques, but not in flowers.1 Publication

    Inductioni

    Not induced by environmental stresses such as dehydration, salinity and low temperature.

    Gene expression databases

    GenevestigatoriQ56W08.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT4G38530.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ56W08.
    SMRiQ56W08. Positions 26-557.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini19 – 5436EF-handAdd
    BLAST
    Domaini106 – 250145PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini296 – 412117PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini415 – 523109C2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi67 – 8216His-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 EF-hand domain.Curated
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG149692.
    HOGENOMiHOG000244119.
    InParanoidiQ56W08.
    KOiK05857.
    OMAiIAIHAAN.
    PhylomeDBiQ56W08.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view]
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q56W08-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSESFKVCFC CSRSFKEKTR QPPVSIKRLF EAYSRNGKMS FDELLRFVSE    50
    VQGERHAGLD YVQDIFHSVK HHNVFHHHGL VHLNAFYRYL FSDTNSPLPM 100
    SGQVHHDMKA PLSHYFVYTG HNSYLTGNQV NSRSSVEPIV QALRKGVKVI 150
    ELDLWPNPSG NAAEVRHGRT LTSHEDLQKC LTAIKDNAFH VSDYPVIITL 200
    EDHLPPKLQA QVAKMLTKTY RGMLFRRVSE SFKHFPSPEE LKGKILISTK 250
    PPKEYLESKT VHTTRTPTVK ETSWNRVANK ILEEYKDMES EAVGYRDLIA 300
    IHAANCKDPS KDCLSDDPEK PIRVSMDEQW LDTMVRTRGT DLVRFTQRNL 350
    VRIYPKGTRV DSSNYDPHVG WTHGAQMVAF NMQGHGKQLW IMQGMFRGNG 400
    GCGYVKKPRI LLDEHTLFDP CKRFPIKTTL KVKIYTGEGW DLDFHHTHFD 450
    QYSPPDFFVK IGIAGVPRDT VSYRTETAVD QWFPIWGNDE FLFQLSVPEL 500
    ALLWFKVQDY DNDTQNDFAG QTCLPLPELK SGVRAVRLHD RTGKAYKNTR 550
    LLVSFALDPP YTFR 564
    Length:564
    Mass (Da):64,944
    Last modified:May 10, 2005 - v1
    Checksum:i6EA1067C8864345A
    GO

    Sequence cautioni

    The sequence AAC48991.1 differs from that shown. Reason: Frameshift at positions 535 and 543.
    The sequence AAC48991.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence CAB37509.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAB80517.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti526 – 5261L → R in AAC48991. (PubMed:7716237)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL035540 Genomic DNA. Translation: CAB37509.1. Sequence problems.
    AL161593 Genomic DNA. Translation: CAB80517.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE86943.1.
    AK222239 mRNA. Translation: BAD95426.1.
    U13203 mRNA. Translation: AAC48991.1. Sequence problems.
    PIRiT05681.
    RefSeqiNP_195565.2. NM_120014.4.
    UniGeneiAt.2775.

    Genome annotation databases

    EnsemblPlantsiAT4G38530.1; AT4G38530.1; AT4G38530.
    GeneIDi830010.
    KEGGiath:AT4G38530.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL035540 Genomic DNA. Translation: CAB37509.1 . Sequence problems.
    AL161593 Genomic DNA. Translation: CAB80517.1 . Sequence problems.
    CP002687 Genomic DNA. Translation: AEE86943.1 .
    AK222239 mRNA. Translation: BAD95426.1 .
    U13203 mRNA. Translation: AAC48991.1 . Sequence problems.
    PIRi T05681.
    RefSeqi NP_195565.2. NM_120014.4.
    UniGenei At.2775.

    3D structure databases

    ProteinModelPortali Q56W08.
    SMRi Q56W08. Positions 26-557.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT4G38530.1-P.

    Proteomic databases

    PRIDEi Q56W08.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G38530.1 ; AT4G38530.1 ; AT4G38530 .
    GeneIDi 830010.
    KEGGi ath:AT4G38530.

    Organism-specific databases

    TAIRi AT4G38530.

    Phylogenomic databases

    eggNOGi NOG149692.
    HOGENOMi HOG000244119.
    InParanoidi Q56W08.
    KOi K05857.
    OMAi IAIHAAN.
    PhylomeDBi Q56W08.

    Enzyme and pathway databases

    BioCyci ARA:AT4G38530-MONOMER.
    Reactomei REACT_187614. Synthesis of IP3 and IP4 in the cytosol.

    Gene expression databases

    Genevestigatori Q56W08.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view ]
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "An Arabidopsis cDNA related to animal phosphoinositide-specific phospholipase C genes."
      Yamamoto Y.T., Conkling M.A., Sussex I.M., Irish V.F.
      Plant Physiol. 107:1029-1030(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-564.
      Strain: cv. Landsberg erecta.
      Tissue: Shoot.
    5. "Inositol phospholipid metabolism in Arabidopsis. Characterized and putative isoforms of inositol phospholipid kinase and phosphoinositide-specific phospholipase C."
      Mueller-Roeber B., Pical C.
      Plant Physiol. 130:22-46(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, LACK OF INDUCTION, NOMENCLATURE.
    6. "Gene-specific expression and calcium activation of Arabidopsis thaliana phospholipase C isoforms."
      Hunt L., Otterhag L., Lee J.C., Lasheen T., Hunt J., Seki M., Shinozaki K., Sommarin M., Gilmour D.J., Pical C., Gray J.E.
      New Phytol. 162:643-654(2004)
      [AGRICOLA] [Europe PMC]
      Cited for: FUNCTION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiPLCD3_ARATH
    AccessioniPrimary (citable) accession number: Q56W08
    Secondary accession number(s): Q38811, Q9SZN3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: May 10, 2005
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3