Reviewed, UniProtKB/Swiss-Prot Q56UD1 (INV5_ORYSJ)

Names and origin

Protein names

Recommended name:
    Beta-fructofuranosidase, insoluble isoenzyme 5
    EC=3.2.1.26
Alternative name(s):
    Sucrose hydrolase 5
    Invertase 5
    Cell wall beta-fructosidase 5
    OsCIN5

Gene names

Name:	CIN5
Ordered Locus Names:	Os04g0664900, LOC_Os04g56930
ORF Names:	OSJNBa0087O24.4

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	542 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May play a role in stress response.
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Subcellular location	Secreted › extracellular space › apoplast Probable. Secreted › cell wall Probable. Note: Associated to the cell wall Probable.
Tissue specificity	Expressed in roots and leaves. Ref.2
Induction	By rice blast fungus (M.grisea) 48 hours after infection. Down-regulated by sucrose in excised leaves. Ref.2
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.
Sequence caution	The sequence CAE03581.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Apoplast Cell wall Secreted
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-fructofuranosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 542	542	Beta-fructofuranosidase, insoluble isoenzyme 5		PRO_0000169867
Regions
	Region	50 – 53	4	Substrate binding By similarity
	Region	114 – 115	2	Substrate binding By similarity
	Region	178 – 179	2	Substrate binding By similarity
Sites
	Active site	53	1	By similarity
	Binding site	69	1	Substrate By similarity
	Binding site	77	1	Substrate By similarity
	Binding site	233	1	Substrate By similarity
Amino acid modifications
	Glycosylation	142	1	N-linked (GlcNAc...) Potential
	Glycosylation	510	1	N-linked (GlcNAc...) Potential
	Glycosylation	516	1	N-linked (GlcNAc...) Potential
	Disulfide bond	389 ↔ 436		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q56UD1-1 [UniParc]. Last modified April 29, 2008. Version 3. Checksum: C5B64DB46B00FBDE	FASTA	542	59,533
	10 20 30 40 50 60 MQLLVPIYKR HSNSNFKVSS CSSSPMCPVN GKLQLHDGRT AYHFQPAKFW QNDPNGPLYH 70 80 90 100 110 120 NGLYHFFYQY NPHGPLWDTG KLSWGHSVSG DLVNWAFLGT AIDPTDPFDV NGCWSGSATV 130 140 150 160 170 180 LLGGRPAFLY TGRDAGGVQV QNVSFAKNPL DPLLREWEKP SCNPIIAFPA DVINNNFRDP 190 200 210 220 230 240 TTAWLGRDGL WRMVVAAEVA GAGSALVYRS ADFLRWERNA APMHSSAAVP VLECPDFFPV 250 260 270 280 290 300 AEHGIDGLDT SANGGGTGVK HVLKLSEFDT HQDFYMVGRN RRVQWLWVNE YDSKADDVAK 310 320 330 340 350 360 GWAGVQAFPR KVWLDGDGKQ LLQWPVDEIE TLRTKRVGLQ GTEVKAGGLH EIVGVASSQA 370 380 390 400 410 420 DVEVVFEIPN LEDEAESFDP DWLDPHKLCK DKGAASAHGG VGPFGLIVMA SGDLQEQTAV 430 440 450 460 470 480 FFRVFKHHGK YKVFMCTDLT RSSTKADVYK DAYGGFVDVD IQKDKSISLR TLIDHSMIES 490 500 510 520 530 540 FGGGGRACIT TRVYPEHAAT SSSHLYVFNN GSGTVNVSKL EAWEMATATV NSADALDAIT RS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of rice chromosome 4." Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y., Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q., Zhang L. , Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T., Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R., Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G., Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C., Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J., Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J., Li J., Hong G., Xue Y., Han B. Nature 420:316-320(2002) [PubMed: 12447439] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Molecular cloning and expression analysis of the cell-wall invertase gene family in rice (Oryza sativa L.)." Cho J.-I., Lee S.-K., Ko S., Kim H.-K., Jun S.-H., Lee Y.-H., Bhoo S.H., Lee K.-W., An G., Hahn T.-R., Jeon J.-S. Plant Cell Rep. 24:225-236(2005) [PubMed: 15759120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-542, TISSUE SPECIFICITY, INDUCTION. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL606646 Genomic DNA. Translation: CAE03581.1. Sequence problems. AY578162 mRNA. Translation: AAT84405.1. Different initiation.
3D structure databases
ModBase	Search...
Organism-specific databases
Gramene	Q56UD1.
Family and domain databases
InterPro	IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. [Graphical view]
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 2 hits. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INV5_ORYSJ

Accession

Primary (citable) accession number: Q56UD1
Secondary accession number(s): Q7XPK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	April 29, 2008
Last modified:	September 22, 2009
This is version 25 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)