Q56UD0 (INV6_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-fructofuranosidase, insoluble isoenzyme 6
EC=3.2.1.26

Alternative name(s):
Cell wall beta-fructosidase 6
Invertase 6
OsCIN6
Sucrose hydrolase 6

Gene names

Name:	CIN6
Ordered Locus Names:	Os04g0664800, LOC_Os04g56920
ORF Names:	OSJNBa0087O24.3

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Subcellular location	Secreted › extracellular space › apoplast Probable. Secreted › cell wall Probable. Note: Associated to the cell wall Probable.
Tissue specificity	Expressed in roots. Weakly expressed in flowers. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Cellular component	Apoplast Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sucrose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 596	571	Beta-fructofuranosidase, insoluble isoenzyme 6		PRO_0000033383
Regions
	Region	49 – 52	4	Substrate binding By similarity
	Region	113 – 114	2	Substrate binding By similarity
	Region	177 – 178	2	Substrate binding By similarity
Sites
	Active site	52	1	By similarity
	Binding site	68	1	Substrate By similarity
	Binding site	76	1	Substrate By similarity
	Binding site	232	1	Substrate By similarity
Amino acid modifications
	Glycosylation	80	1	N-linked (GlcNAc...) Potential
	Glycosylation	335	1	N-linked (GlcNAc...) Potential
	Glycosylation	556	1	N-linked (GlcNAc...) Potential
	Disulfide bond	436 ↔ 482		By similarity
Experimental info
	Sequence conflict	52 – 54	3	Missing Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q56UD0 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 1FD62385B1389301	FASTA	596	64,743
	10 20 30 40 50 60 MALAGLPLSV FAIAVHFCLV FSSSSSPPVC PANGHRDRTA YHFQPAKNWQ NDPNGPVYYN 70 80 90 100 110 120 GMYHLFYQYN PHGALWDVGN LSWGHSVSGD LVNWAALDNA LDPTAPFDAN GCASGSVTIL 130 140 150 160 170 180 PDGVPVVMYS GIDARRRQVQ NVAFPKNPRD PLLREWTKPG YNPVIPVPAD VSPDNFRDPT 190 200 210 220 230 240 TAWLGSDGLW RFAISAVADG VGATLVYRSA DFLRWERNAA PLHASRDAVM AECPDLFPVA 250 260 270 280 290 300 EHGEDGLDLD ASAIGGAGAG VRHVLKVSMP DTLEDYYMVG RYDDADDTFT VPPEDLEAHG 310 320 330 340 350 360 DDYRRWRRID HGHLYASKTF YDAGKKRRVL WAWVNESDSE ADDVTKGWSG LQSFPRAVWL 370 380 390 400 410 420 DEGGRQLVQW PVEEIETLRR KRGVLLGGNE VEAGGLREIG GIAGSQADVE VAFEIASLAG 430 440 450 460 470 480 ADRLEPDHLR DPDALCGENG AAVHGGIGPF GLLVMASGDL RERTAVFFRV FRLSHGYTVL 490 500 510 520 530 540 MCTDLTRSTS RAGVYKPSHG GFVDIDIEKD RAISLRTLID HSIVESFGGG GRTCMTARVY 550 560 570 580 590 PEHVATGSSH LYVFNNASDA VKVSKLEAWE LATASVNAGD DGLISYGGPV CAAQVQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression analysis of the cell-wall invertase gene family in rice (Oryza sativa L.)." Cho J.-I., Lee S.-K., Ko S., Kim H.-K., Jun S.-H., Lee Y.-H., Bhoo S.H., Lee K.-W., An G., Hahn T.-R., Jeon J.-S. Plant Cell Rep. 24:225-236(2005) [PubMed: 15759120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: cv. Nipponbare.
[2]	"Sequence and analysis of rice chromosome 4." Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y., Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q., Zhang L. , Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T., Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R., Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G., Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C., Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J., Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J., Li J., Hong G., Xue Y., Han B. Nature 420:316-320(2002) [PubMed: 12447439] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY578163 mRNA. Translation: AAT84406.1. AL606646 Genomic DNA. Translation: CAE03580.1.
RefSeq	NP_001054172.2. NM_001060707.2.
UniGene	Os.61724.
3D structure databases
ProteinModelPortal	Q56UD0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q56UD0.
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os04g56920.1; LOC_Os04g56920.1; LOC_Os04g56920.
GeneID	4337312.
KEGG	osa:4337312.
Organism-specific databases
Gramene	Q56UD0.
Phylogenomic databases
GeneTree	EPGT00050000007871.
OMA	DANGCAS.
ProtClustDB	CLSN2695207.
Family and domain databases
InterPro	IPR008985. ConA-like_lec_gl. IPR001362. Glyco_hydro_32. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR023296. Glyco_hydro_43_beta-prop. [Graphical view]
Gene3D	G3DSA:2.115.10.20. Glyco_hydro_43_beta-prop. 1 hit.
KO	K01193.
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INV6_ORYSJ

Accession

Primary (citable) accession number: Q56UD0
Secondary accession number(s): Q7XPK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	May 10, 2005
Last modified:	November 16, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program