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Q56R42

- HOG1_CRYNH

UniProt

Q56R42 - HOG1_CRYNH

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Protein

Mitogen-activated protein kinase HOG1

Gene

HOG1

Organism
Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription of target genes. Also involved in response to UV radiations, heat stress and oxidative stress. Mediates the sensitivity to fludioxonil, an agricultural fungicide.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by dephosphorylation after osmotic stress or in presence of the fludioxonil fungicide.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei49 – 491ATPPROSITE-ProRule annotation
Active sitei141 – 1411Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 349ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase HOG1 (EC:2.7.11.24)
Short name:
MAP kinase HOG1
Gene namesi
Name:HOG1
ORF Names:CNAG_01523
OrganismiCryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
Taxonomic identifieri235443 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTremellaceaeFilobasidiellaFilobasidiella/Cryptococcus neoformans species complex
ProteomesiUP000010091: Chromosome 11

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication
Note: Concentration into the nucleus in response to hyperosmotic conditions is transient and moderate.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi49 – 491K → S: Confers resistance to fludioxonil; when associated with N-50. 1 Publication
Mutagenesisi50 – 501K → N: Confers resistance to fludioxonil; when associated with S-49. 1 Publication
Mutagenesisi171 – 1711T → A: Confers resistance to fludioxonil; when associated with A-173. 1 Publication
Mutagenesisi173 – 1731Y → A: Confers resistance to fludioxonil; when associated with A-171. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 365365Mitogen-activated protein kinase HOG1PRO_0000289685Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei171 – 1711PhosphothreonineBy similarity
Modified residuei173 – 1731PhosphotyrosineBy similarity

Post-translational modificationi

Contrary to what happens in most fungi, is constitutively phosphorylated during non-stress conditions and dephosphorylated after osmotic stress or in presence of the fludioxonil fungicide.3 Publications

Keywords - PTMi

Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliQ56R42.
SMRiQ56R42. Positions 3-342.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 299280Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi171 – 1733TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily. HOG1 sub-subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

OrthoDBiEOG7K3TWD.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01773. P38MAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q56R42-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADFVKLSIF GTVFEVTTRY VDLQPVGMGA FGLVCSAKDQ LSGTSVAIKK
60 70 80 90 100
IMKPFSTPVL SKRTYRELKL LKHLRHENII SLSDIFISPL EDIYFVTELL
110 120 130 140 150
GTDLHRLLTS RPLEKQFIQY FLYQILRGLK YVHSAGVVHR DLKPSNILVN
160 170 180 190 200
ENCDLKICDF GLARIQDPQM TGYVSTRYYR APEIMLTWQK YDVAVDIWST
210 220 230 240 250
GCIFAEMLEG KPLFPGKDHV NQFSIITELL GTPPDDVIQT IASENTLRFV
260 270 280 290 300
QSLPKREKVP FSTKFPNADP VSLDLLEKML VFDPRTRISA AEGLAHEYLA
310 320 330 340 350
PYHDPTDEPV AAEVFDWSFN DADLPVDTWK VMMYSEILDF HNLGDISQNE
360
AEGPVTGEVP AAPAS
Length:365
Mass (Da):41,213
Last modified:May 10, 2005 - v1
Checksum:iF28E2E95B6DAD6B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY775548 Genomic DNA. Translation: AAX08139.1.
CP003830 Genomic DNA. Translation: AFR97728.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY775548 Genomic DNA. Translation: AAX08139.1 .
CP003830 Genomic DNA. Translation: AFR97728.1 .

3D structure databases

ProteinModelPortali Q56R42.
SMRi Q56R42. Positions 3-342.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

OrthoDBi EOG7K3TWD.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR01773. P38MAPKINASE.
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Specialization of the HOG pathway and its impact on differentiation and virulence of Cryptococcus neoformans."
    Bahn Y.-S., Kojima K., Cox G.M., Heitman J.
    Mol. Biol. Cell 16:2285-2300(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PHOSPHORYLATION, SUBCELLULAR LOCATION.
    Strain: H99 / ATCC 208821 / CBS 10515 / FGSC 9487.
  2. "Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation."
    Janbon G., Ormerod K.L., Paulet D., Byrnes E.J. III, Yadav V., Chatterjee G., Mullapudi N., Hon C.-C., Billmyre R.B., Brunel F., Bahn Y.-S., Chen W., Chen Y., Chow E.W.L., Coppee J.-Y., Floyd-Averette A., Gaillardin C., Gerik K.J.
    , Goldberg J., Gonzalez-Hilarion S., Gujja S., Hamlin J.L., Hsueh Y.-P., Ianiri G., Jones S., Kodira C.D., Kozubowski L., Lam W., Marra M., Mesner L.D., Mieczkowski P.A., Moyrand F., Nielsen K., Proux C., Rossignol T., Schein J.E., Sun S., Wollschlaeger C., Wood I.A., Zeng Q., Neuveglise C., Newlon C.S., Perfect J.R., Lodge J.K., Idnurm A., Stajich J.E., Kronstad J.W., Sanyal K., Heitman J., Fraser J.A., Cuomo C.A., Dietrich F.S.
    PLoS Genet. 10:E1004261-E1004261(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: H99 / ATCC 208821 / CBS 10515 / FGSC 9487.
  3. "Calcineurin, Mpk1 and Hog1 MAPK pathways independently control fludioxonil antifungal sensitivity in Cryptococcus neoformans."
    Kojima K., Bahn Y.-S., Heitman J.
    Microbiology 152:591-604(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION, MUTAGENESIS OF LYS-49; LYS-50; THR-171 AND TYR-173.
  4. "A unique fungal two-component system regulates stress responses, drug sensitivity, sexual development, and virulence of Cryptococcus neoformans."
    Bahn Y.-S., Kojima K., Cox G.M., Heitman J.
    Mol. Biol. Cell 17:3122-3135(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION.

Entry informationi

Entry nameiHOG1_CRYNH
AccessioniPrimary (citable) accession number: Q56R42
Secondary accession number(s): J9VTY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 10, 2005
Last modified: November 26, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3