Reviewed, UniProtKB/Swiss-Prot Q56R42 (HOG1_CRYNV)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase HOG1
      Short name=MAP kinase HOG1
    EC=2.7.11.24

Gene names

Name:

HOG1

Organism

Cryptococcus neoformans var. grubii (Filobasidiella neoformans var. grubii)

Taxonomic identifier

178876 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Mitogen-activated protein kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment. Controls osmotic regulation of transcription of target genes. Also involved in response to UV radiations, heat stress and oxidative stress. Mediates the sensitivity to fludioxonil, an agricultural fungicide. Ref.1 Ref.2 Ref.3
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by dephosphorylation after osmotic stress or in presence of the fludioxonil fungicide.
Subcellular location	Cytoplasm. Nucleus. Note: Concentration into the nucleus in response to hyperosmotic conditions is transient and moderate. Ref.1
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Contrary to what happens in most fungi, is constitutively phosphorylated during non-stress conditions and dephosphorylated after osmotic stress or in presence of the fludioxonil fungicide. Ref.1 Ref.2 Ref.3
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily. HOG1 sub-subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Mitogen-activated protein kinase HOG1		PRO_0000289685
Regions
	Domain	20 – 299	280	Protein kinase
	Nucleotide binding	26 – 34	9	ATP By similarity
	Motif	171 – 173	3	TXY
Sites
	Active site	141	1	Proton acceptor By similarity
	Binding site	49	1	ATP By similarity
Amino acid modifications
	Modified residue	171	1	Phosphothreonine By similarity
	Modified residue	173	1	Phosphotyrosine By similarity
Experimental info
	Mutagenesis	49	1	K → S: Confers resistance to fludioxonil; when associated with N-50. Ref.2
	Mutagenesis	50	1	K → N: Confers resistance to fludioxonil; when associated with S-49. Ref.2
	Mutagenesis	171	1	T → A: Confers resistance to fludioxonil; when associated with A-173. Ref.2
	Mutagenesis	173	1	Y → A: Confers resistance to fludioxonil; when associated with A-171. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q56R42-1 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: F28E2E95B6DAD6B5	FASTA	365	41,213
	10 20 30 40 50 60 MADFVKLSIF GTVFEVTTRY VDLQPVGMGA FGLVCSAKDQ LSGTSVAIKK IMKPFSTPVL 70 80 90 100 110 120 SKRTYRELKL LKHLRHENII SLSDIFISPL EDIYFVTELL GTDLHRLLTS RPLEKQFIQY 130 140 150 160 170 180 FLYQILRGLK YVHSAGVVHR DLKPSNILVN ENCDLKICDF GLARIQDPQM TGYVSTRYYR 190 200 210 220 230 240 APEIMLTWQK YDVAVDIWST GCIFAEMLEG KPLFPGKDHV NQFSIITELL GTPPDDVIQT 250 260 270 280 290 300 IASENTLRFV QSLPKREKVP FSTKFPNADP VSLDLLEKML VFDPRTRISA AEGLAHEYLA 310 320 330 340 350 360 PYHDPTDEPV AAEVFDWSFN DADLPVDTWK VMMYSEILDF HNLGDISQNE AEGPVTGEVP AAPAS

References

[1]	"Specialization of the HOG pathway and its impact on differentiation and virulence of Cryptococcus neoformans." Bahn Y.-S., Kojima K., Cox G.M., Heitman J. Mol. Biol. Cell 16:2285-2300(2005) [PubMed: 15728721] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PHOSPHORYLATION, SUBCELLULAR LOCATION. Strain: H99.
[2]	"Calcineurin, Mpk1 and Hog1 MAPK pathways independently control fludioxonil antifungal sensitivity in Cryptococcus neoformans." Kojima K., Bahn Y.-S., Heitman J. Microbiology 152:591-604(2006) [PubMed: 16514140] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION, MUTAGENESIS OF LYS-49; LYS-50; THR-171 AND TYR-173.
[3]	"A unique fungal two-component system regulates stress responses, drug sensitivity, sexual development, and virulence of Cryptococcus neoformans." Bahn Y.-S., Kojima K., Cox G.M., Heitman J. Mol. Biol. Cell 17:3122-3135(2006) [PubMed: 16672377] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY775548 Genomic DNA. Translation: AAX08139.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.11.24. 303268.
Family and domain databases
InterPro	IPR003527. MAP_kinase_CS. IPR008352. MAPK_p38. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR01773. P38MAPKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOG1_CRYNV

Accession

Primary (citable) accession number: Q56R42

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	May 10, 2005
Last modified:	September 22, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)