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Protein

Importin subunit alpha-6

Gene

Kpna5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates nuclear import of STAT1 homodimers and STAT1/STAT2 heterodimers by recognizing non-classical NLSs of STAT1 and STAT2 through ARM repeats 8-9 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Importin subunit alpha-6
Alternative name(s):
Karyopherin subunit alpha-5
Gene namesi
Name:Kpna5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1561324 Kpna5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003260831 – 536Importin subunit alpha-6Add BLAST536

Proteomic databases

PaxDbiQ56R16
PRIDEiQ56R16

PTM databases

PhosphoSitePlusiQ56R16

Interactioni

Subunit structurei

Forms a complex with importin subunit beta-1.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039571

Structurei

3D structure databases

ProteinModelPortaliQ56R16
SMRiQ56R16
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 57IBBPROSITE-ProRule annotationAdd BLAST56
Repeati73 – 115ARM 1; truncatedAdd BLAST43
Repeati116 – 159ARM 2Add BLAST44
Repeati160 – 204ARM 3Add BLAST45
Repeati205 – 243ARM 4Add BLAST39
Repeati244 – 288ARM 5Add BLAST45
Repeati289 – 328ARM 6Add BLAST40
Repeati329 – 370ARM 7Add BLAST42
Repeati371 – 410ARM 8Add BLAST40
Repeati411 – 453ARM 9Add BLAST43
Repeati457 – 502ARM 10; atypicalAdd BLAST46

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni147 – 239NLS binding site (major)By similarityAdd BLAST93
Regioni316 – 404NLS binding site (minor)By similarityAdd BLAST89

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi42 – 51Nuclear localization signalBy similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi25 – 28Poly-Arg4

Domaini

Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import (By similarity).By similarity
The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins (By similarity).By similarity
The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding (By similarity).By similarity

Sequence similaritiesi

Belongs to the importin alpha family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0166 Eukaryota
COG5064 LUCA
HOGENOMiHOG000167616
HOVERGENiHBG001846
InParanoidiQ56R16
OrthoDBiEOG091G095Z
PhylomeDBiQ56R16
TreeFamiTF354205

Family and domain databases

Gene3Di1.20.5.690, 1 hit
1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR032413 Arm_3
IPR000225 Armadillo
IPR002652 Importin-a_IBB
IPR036975 Importin-a_IBB_sf
IPR024931 Importing_su_alpha
PfamiView protein in Pfam
PF00514 Arm, 8 hits
PF16186 Arm_3, 1 hit
PF01749 IBB, 1 hit
PIRSFiPIRSF005673 Importin_alpha, 1 hit
SMARTiView protein in SMART
SM00185 ARM, 8 hits
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50176 ARM_REPEAT, 2 hits
PS51214 IBB, 1 hit

Sequencei

Sequence statusi: Complete.

Q56R16-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPGKDNYR MKSYKNKALN PQEMRRRREE EGIQLRKQKR EEQLFKRRNV
60 70 80 90 100
SLPRNDDCML ESPIQDPDVS STVPIPEEDM ITADMIQMIF SNNAEQQLTA
110 120 130 140 150
TQKFRKLLSK EPNPPIDQVI QKPGVVQRFV KFLERNENCT LQFEAAWALT
160 170 180 190 200
NIASGTFLHT KVVIETGAVP IFIRLLTSEH EDVQEQAVWA LGNIAGDNAE
210 220 230 240 250
CRDFVLNCEI LPPLLELLTN SNRLTTTRNA VWALSNLCRG KNPPPNFSKV
260 270 280 290 300
SPCLNVLSRL LFSSDPDVLA DVCWALSYLS DGPNDKIQVV IDSGVCRRLV
310 320 330 340 350
ELLMHNDYKV VSPALRAVGN IVTGDDIQTQ VILNCSALPC LLHLLGSPKE
360 370 380 390 400
SVRKEACWTI SNITAGNRMQ IQAVIDGSIF PVLIEVLQKA EFRTRKEAAW
410 420 430 440 450
AITNATSGGA PEQIRYLVTL GCIKPLCDLL TVMDSKIVQV ALNGLENILR
460 470 480 490 500
LGERESKQNG VGINPYCALI EEAYGLDKIE FLQSHENQEI YQKAFDLIER
510 520 530
YFGVEEDDPS LVPQVDEQQR QFLFQQCEAP GEGFQL
Length:536
Mass (Da):60,299
Last modified:May 10, 2005 - v1
Checksum:iF0602F130BBB812A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY779029 mRNA Translation: AAX07456.1
RefSeqiNP_001020284.1, NM_001025113.1
UniGeneiRn.138147

Genome annotation databases

GeneIDi294392
KEGGirno:294392
UCSCiRGD:1561324 rat

Similar proteinsi

Entry informationi

Entry nameiIMA6_RAT
AccessioniPrimary (citable) accession number: Q56R16
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: May 10, 2005
Last modified: March 28, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health