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Protein

Importin subunit alpha-6

Gene

Kpna5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates nuclear import of STAT1 homodimers and STAT1/STAT2 heterodimers by recognizing non-classical NLSs of STAT1 and STAT2 through ARM repeats 8-9 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Importin subunit alpha-6
Alternative name(s):
Karyopherin subunit alpha-5
Gene namesi
Name:Kpna5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1561324. Kpna5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 536536Importin subunit alpha-6PRO_0000326083Add
BLAST

Proteomic databases

PaxDbiQ56R16.
PRIDEiQ56R16.

PTM databases

PhosphoSiteiQ56R16.

Interactioni

Subunit structurei

Forms a complex with importin subunit beta-1.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039571.

Structurei

3D structure databases

ProteinModelPortaliQ56R16.
SMRiQ56R16. Positions 9-51, 82-505.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 5756IBBPROSITE-ProRule annotationAdd
BLAST
Repeati73 – 11543ARM 1; truncatedAdd
BLAST
Repeati116 – 15944ARM 2Add
BLAST
Repeati160 – 20445ARM 3Add
BLAST
Repeati205 – 24339ARM 4Add
BLAST
Repeati244 – 28845ARM 5Add
BLAST
Repeati289 – 32840ARM 6Add
BLAST
Repeati329 – 37042ARM 7Add
BLAST
Repeati371 – 41040ARM 8Add
BLAST
Repeati411 – 45343ARM 9Add
BLAST
Repeati457 – 50246ARM 10; atypicalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni147 – 23993NLS binding site (major)By similarityAdd
BLAST
Regioni316 – 40489NLS binding site (minor)By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5110Nuclear localization signalBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi25 – 284Poly-Arg

Domaini

Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import (By similarity).By similarity
The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins (By similarity).By similarity
The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding (By similarity).By similarity

Sequence similaritiesi

Belongs to the importin alpha family.Curated
Contains 10 ARM repeats.PROSITE-ProRule annotation
Contains 1 IBB domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0166. Eukaryota.
COG5064. LUCA.
HOGENOMiHOG000167616.
HOVERGENiHBG001846.
InParanoidiQ56R16.
OMAiADMIQMI.
OrthoDBiEOG7VHSWV.
PhylomeDBiQ56R16.
TreeFamiTF354205.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR032413. Arm_3.
IPR000225. Armadillo.
IPR002652. Importin-a_IBB.
IPR024931. Importing_su_alpha.
[Graphical view]
PfamiPF00514. Arm. 8 hits.
PF16186. Arm_3. 1 hit.
PF01749. IBB. 1 hit.
[Graphical view]
PIRSFiPIRSF005673. Importin_alpha. 1 hit.
SMARTiSM00185. ARM. 8 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 2 hits.
PS51214. IBB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q56R16-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPGKDNYR MKSYKNKALN PQEMRRRREE EGIQLRKQKR EEQLFKRRNV
60 70 80 90 100
SLPRNDDCML ESPIQDPDVS STVPIPEEDM ITADMIQMIF SNNAEQQLTA
110 120 130 140 150
TQKFRKLLSK EPNPPIDQVI QKPGVVQRFV KFLERNENCT LQFEAAWALT
160 170 180 190 200
NIASGTFLHT KVVIETGAVP IFIRLLTSEH EDVQEQAVWA LGNIAGDNAE
210 220 230 240 250
CRDFVLNCEI LPPLLELLTN SNRLTTTRNA VWALSNLCRG KNPPPNFSKV
260 270 280 290 300
SPCLNVLSRL LFSSDPDVLA DVCWALSYLS DGPNDKIQVV IDSGVCRRLV
310 320 330 340 350
ELLMHNDYKV VSPALRAVGN IVTGDDIQTQ VILNCSALPC LLHLLGSPKE
360 370 380 390 400
SVRKEACWTI SNITAGNRMQ IQAVIDGSIF PVLIEVLQKA EFRTRKEAAW
410 420 430 440 450
AITNATSGGA PEQIRYLVTL GCIKPLCDLL TVMDSKIVQV ALNGLENILR
460 470 480 490 500
LGERESKQNG VGINPYCALI EEAYGLDKIE FLQSHENQEI YQKAFDLIER
510 520 530
YFGVEEDDPS LVPQVDEQQR QFLFQQCEAP GEGFQL
Length:536
Mass (Da):60,299
Last modified:May 10, 2005 - v1
Checksum:iF0602F130BBB812A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY779029 mRNA. Translation: AAX07456.1.
RefSeqiNP_001020284.1. NM_001025113.1.
UniGeneiRn.138147.

Genome annotation databases

GeneIDi294392.
KEGGirno:294392.
UCSCiRGD:1561324. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY779029 mRNA. Translation: AAX07456.1.
RefSeqiNP_001020284.1. NM_001025113.1.
UniGeneiRn.138147.

3D structure databases

ProteinModelPortaliQ56R16.
SMRiQ56R16. Positions 9-51, 82-505.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039571.

PTM databases

PhosphoSiteiQ56R16.

Proteomic databases

PaxDbiQ56R16.
PRIDEiQ56R16.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi294392.
KEGGirno:294392.
UCSCiRGD:1561324. rat.

Organism-specific databases

CTDi3841.
RGDi1561324. Kpna5.

Phylogenomic databases

eggNOGiKOG0166. Eukaryota.
COG5064. LUCA.
HOGENOMiHOG000167616.
HOVERGENiHBG001846.
InParanoidiQ56R16.
OMAiADMIQMI.
OrthoDBiEOG7VHSWV.
PhylomeDBiQ56R16.
TreeFamiTF354205.

Miscellaneous databases

NextBioi638106.
PROiQ56R16.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR032413. Arm_3.
IPR000225. Armadillo.
IPR002652. Importin-a_IBB.
IPR024931. Importing_su_alpha.
[Graphical view]
PfamiPF00514. Arm. 8 hits.
PF16186. Arm_3. 1 hit.
PF01749. IBB. 1 hit.
[Graphical view]
PIRSFiPIRSF005673. Importin_alpha. 1 hit.
SMARTiSM00185. ARM. 8 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 2 hits.
PS51214. IBB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Transcriptomic and phylogenetic analysis of Kpna genes: a family of nuclear import factors modulated in xenobiotic-mediated liver growth."
    Plant K.E., Everett D.M., Gordon Gibson G., Lyon J., Plant N.J.
    Pharmacogenet. Genomics 16:647-658(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Testis.

Entry informationi

Entry nameiIMA6_RAT
AccessioniPrimary (citable) accession number: Q56R16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: May 10, 2005
Last modified: May 11, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.