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Q56P03

- EAPP_HUMAN

UniProt

Q56P03 - EAPP_HUMAN

Protein

E2F-associated phosphoprotein

Gene

EAPP

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 4 (18 May 2010)
      Previous versions | rss
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    Functioni

    May play an important role in the fine-tuning of both major E2F1 activities, the regulation of the cell-cycle and the induction of apoptosis. Promotes S-phase entry, and inhibits p14(ARP) expression.1 Publication

    GO - Biological processi

    1. negative regulation of transcription elongation from RNA polymerase II promoter Source: MGI
    2. positive regulation of cell proliferation Source: MGI
    3. positive regulation of transcription elongation from RNA polymerase II promoter Source: MGI

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    E2F-associated phosphoprotein
    Short name:
    EAPP
    Gene namesi
    Name:EAPP
    Synonyms:C14orf11
    ORF Names:BM-036
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 14

    Organism-specific databases

    HGNCiHGNC:19312. EAPP.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: HPA
    2. Golgi apparatus Source: HPA
    3. nucleus Source: HPA

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162384208.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 285285E2F-associated phosphoproteinPRO_0000086904Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication
    Modified residuei17 – 171Phosphoserine2 Publications
    Modified residuei109 – 1091Phosphoserine5 Publications
    Modified residuei111 – 1111Phosphoserine5 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ56P03.
    PaxDbiQ56P03.
    PRIDEiQ56P03.

    PTM databases

    PhosphoSiteiQ56P03.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed. Highest levels in heart, placenta, skeletal muscle and pancreas. Lower levels in brain, lung and kidney. In the brain, expressed in all regions with high levels in the cerebellum and cerebral cortex. Expressed in COS1 and transformed skin fibroblasts.2 Publications

    Developmental stagei

    During the cell cycle, expression disappears during mitosis.

    Gene expression databases

    ArrayExpressiQ56P03.
    BgeeiQ56P03.
    CleanExiHS_EAPP.
    GenevestigatoriQ56P03.

    Organism-specific databases

    HPAiHPA002916.

    Interactioni

    Subunit structurei

    Interacts with E2F1. The C-terminal half binds the N-terminal of E2F1. Also interacts with E2F2 and E2F3, but not E2F4.1 Publication

    Protein-protein interaction databases

    BioGridi120941. 6 interactions.
    IntActiQ56P03. 5 interactions.
    MINTiMINT-2874613.
    STRINGi9606.ENSP00000250454.

    Structurei

    3D structure databases

    ProteinModelPortaliQ56P03.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi122 – 1276Poly-Lys

    Phylogenomic databases

    eggNOGiNOG250977.
    HOGENOMiHOG000068005.
    HOVERGENiHBG054593.
    InParanoidiQ56P03.
    OMAiRGHKKMR.
    OrthoDBiEOG7SR4ND.
    PhylomeDBiQ56P03.
    TreeFamiTF328497.

    Family and domain databases

    InterProiIPR019370. E2F-assoc_phosphoprotein.
    [Graphical view]
    PfamiPF10238. Eapp_C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q56P03-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNRLPDDYDP YAVEEPSDEE PALSSSEDEV DVLLHGTPDQ KRKLIRECLT    50
    GESESSSEDE FEKEMEAELN STMKTMEDKL SSLGTGSSSG NGKVATAPTR 100
    YYDDIYFDSD SEDEDRAVQV TKKKKKKQHK IPTNDELLYD PEKDNRDQAW 150
    VDAQRRGYHG LGPQRSRQQQ PVPNSDAVLN CPACMTTLCL DCQRHESYKT 200
    QYRAMFVMNC SINKEEVLRY KASENRKKRR VHKKMRSNRE DAAEKAETDV 250
    EEIYHPVMCT ECSTEVAVYD KDEVFHFFNV LASHS 285
    Length:285
    Mass (Da):32,762
    Last modified:May 18, 2010 - v4
    Checksum:i1C0691602FFB516A
    GO

    Sequence cautioni

    The sequence AAF67623.1 differs from that shown. Reason: Frameshift at positions 178, 189, 198 and 226.
    The sequence AAO17041.1 differs from that shown. Reason: Frameshift at positions 178, 189, 198 and 226.
    The sequence AAO17042.1 differs from that shown. Reason: Frameshift at positions 178, 189, 198 and 226.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti3 – 31R → L in AAX63200. (PubMed:15716352)Curated
    Sequence conflicti52 – 521E → G in BAA91271. (PubMed:14702039)Curated
    Sequence conflicti239 – 2391R → Q in AAX63200. (PubMed:15716352)Curated
    Sequence conflicti239 – 2391R → Q in AAH01245. (PubMed:15489334)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti168 – 1681Q → E.2 Publications
    Corresponds to variant rs17856038 [ dbSNP | Ensembl ].
    VAR_031915

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY157300 mRNA. Translation: AAO17041.1. Frameshift.
    AY157301 mRNA. Translation: AAO17042.1. Frameshift.
    AY869694 mRNA. Translation: AAX63200.1.
    AF217512 mRNA. Translation: AAF67623.1. Frameshift.
    AK000585 mRNA. Translation: BAA91271.1.
    AL445363 Genomic DNA. No translation available.
    BC001245 mRNA. Translation: AAH01245.1.
    CCDSiCCDS41941.1.
    RefSeqiNP_060923.2. NM_018453.3.
    UniGeneiHs.433269.

    Genome annotation databases

    EnsembliENST00000250454; ENSP00000250454; ENSG00000129518.
    GeneIDi55837.
    KEGGihsa:55837.
    UCSCiuc001wsd.1. human.

    Polymorphism databases

    DMDMi296439474.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY157300 mRNA. Translation: AAO17041.1 . Frameshift.
    AY157301 mRNA. Translation: AAO17042.1 . Frameshift.
    AY869694 mRNA. Translation: AAX63200.1 .
    AF217512 mRNA. Translation: AAF67623.1 . Frameshift.
    AK000585 mRNA. Translation: BAA91271.1 .
    AL445363 Genomic DNA. No translation available.
    BC001245 mRNA. Translation: AAH01245.1 .
    CCDSi CCDS41941.1.
    RefSeqi NP_060923.2. NM_018453.3.
    UniGenei Hs.433269.

    3D structure databases

    ProteinModelPortali Q56P03.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 120941. 6 interactions.
    IntActi Q56P03. 5 interactions.
    MINTi MINT-2874613.
    STRINGi 9606.ENSP00000250454.

    PTM databases

    PhosphoSitei Q56P03.

    Polymorphism databases

    DMDMi 296439474.

    Proteomic databases

    MaxQBi Q56P03.
    PaxDbi Q56P03.
    PRIDEi Q56P03.

    Protocols and materials databases

    DNASUi 55837.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000250454 ; ENSP00000250454 ; ENSG00000129518 .
    GeneIDi 55837.
    KEGGi hsa:55837.
    UCSCi uc001wsd.1. human.

    Organism-specific databases

    CTDi 55837.
    GeneCardsi GC14M034985.
    HGNCi HGNC:19312. EAPP.
    HPAi HPA002916.
    MIMi 609486. gene.
    neXtProti NX_Q56P03.
    PharmGKBi PA162384208.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG250977.
    HOGENOMi HOG000068005.
    HOVERGENi HBG054593.
    InParanoidi Q56P03.
    OMAi RGHKKMR.
    OrthoDBi EOG7SR4ND.
    PhylomeDBi Q56P03.
    TreeFami TF328497.

    Miscellaneous databases

    GeneWikii EAPP.
    GenomeRNAii 55837.
    NextBioi 61061.
    PROi Q56P03.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q56P03.
    Bgeei Q56P03.
    CleanExi HS_EAPP.
    Genevestigatori Q56P03.

    Family and domain databases

    InterProi IPR019370. E2F-assoc_phosphoprotein.
    [Graphical view ]
    Pfami PF10238. Eapp_C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Defining a holoprosencephaly locus on human chromosome 14q13 and characterization of potential candidate genes."
      Kamnasaran D., Chen C.-P., Devriendt K., Mehta L., Cox D.W.
      Genomics 85:608-621(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Fetal brain.
    2. "EAPP, a novel E2F binding protein that modulates E2F-dependent transcription."
      Novy M., Pohn R., Andorfer P., Novy-Weiland T., Galos B., Schwarzmayr L., Rotheneder H.
      Mol. Biol. Cell 16:2181-2190(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, PHOSPHORYLATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH E2F1; E2F2 AND E2F3, VARIANT GLU-168.
    3. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
      Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
      , Gu J., Chen S.-J., Chen Z.
      Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Bone marrow.
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "The DNA sequence and analysis of human chromosome 14."
      Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H.
      , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
      Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-168.
      Tissue: Cervix.
    7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-109 AND SER-111, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-109 AND SER-111, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    9. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17; SER-109 AND SER-111, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-109 AND SER-111, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiEAPP_HUMAN
    AccessioniPrimary (citable) accession number: Q56P03
    Secondary accession number(s): Q9BVF4, Q9NWV5, Q9NZ86
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 30, 2005
    Last sequence update: May 18, 2010
    Last modified: October 1, 2014
    This is version 85 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 14
      Human chromosome 14: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3