Q56P03 (EAPP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E2F-associated phosphoprotein
Short name=EAPP

Gene names

Name:	EAPP
Synonyms:	C14orf11
ORF Names:	BM-036

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play an important role in the fine-tuning of both major E2F1 activities, the regulation of the cell-cycle and the induction of apoptosis. Promotes S-phase entry, and inhibits p14(ARP) expression. Ref.2
Subunit structure	Interacts with E2F1. The C-terminal half binds the N-terminal of E2F1. Also interacts with E2F2 and E2F3, but not E2F4. Ref.2
Subcellular location	Cytoplasm. Nucleus Ref.1 Ref.2.
Tissue specificity	Ubiquitously expressed. Highest levels in heart, placenta, skeletal muscle and pancreas. Lower levels in brain, lung and kidney. In the brain, expressed in all regions with high levels in the cerebellum and cerebral cortex. Expressed in COS1 and transformed skin fibroblasts. Ref.1 Ref.2
Developmental stage	During the cell cycle, expression disappears during mitosis.
Sequence caution	The sequence AAF67623.1 differs from that shown. Reason: Frameshift at positions 178, 189, 198 and 226. The sequence AAO17041.1 differs from that shown. Reason: Frameshift at positions 178, 189, 198 and 226. The sequence AAO17042.1 differs from that shown. Reason: Frameshift at positions 178, 189, 198 and 226.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of transcription elongation from RNA polymerase II promoter Inferred from direct assay Ref.2. Source: MGI positive regulation of cell proliferation Inferred from direct assay Ref.2. Source: MGI positive regulation of transcription elongation from RNA polymerase II promoter Inferred from direct assay Ref.2. Source: MGI
Cellular component	Golgi apparatus Inferred from direct assay. Source: HPA nucleus Inferred from direct assay Ref.2. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	E2F-associated phosphoprotein		PRO_0000086904
Regions
	Compositional bias	122 – 127	6	Poly-Lys
Amino acid modifications
	Modified residue	17	1	Phosphoserine Ref.7
	Modified residue	24	1	Phosphoserine Ref.7
	Modified residue	25	1	Phosphoserine Ref.7
	Modified residue	26	1	Phosphoserine Ref.7
	Modified residue	37	1	Phosphothreonine Ref.7
	Modified residue	50	1	Phosphothreonine Ref.7
	Modified residue	53	1	Phosphoserine Ref.7
	Modified residue	55	1	Phosphoserine Ref.7
	Modified residue	56	1	Phosphoserine Ref.7
	Modified residue	57	1	Phosphoserine Ref.7
	Modified residue	109	1	Phosphoserine Ref.7 Ref.8 Ref.9
	Modified residue	111	1	Phosphoserine Ref.7 Ref.8 Ref.9
Natural variations
	Natural variant	168	1	Q → E. Ref.2 Ref.6 Corresponds to variant rs17856038 [ dbSNP | Ensembl ].		VAR_031915
Experimental info
	Sequence conflict	3	1	R → L in AAX63200. Ref.2
	Sequence conflict	52	1	E → G in BAA91271. Ref.4
	Sequence conflict	239	1	R → Q in AAX63200. Ref.2
	Sequence conflict	239	1	R → Q in AAH01245. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q56P03 [UniParc]. Last modified May 18, 2010. Version 4. Checksum: 1C0691602FFB516A	FASTA	285	32,762
	10 20 30 40 50 60 MNRLPDDYDP YAVEEPSDEE PALSSSEDEV DVLLHGTPDQ KRKLIRECLT GESESSSEDE 70 80 90 100 110 120 FEKEMEAELN STMKTMEDKL SSLGTGSSSG NGKVATAPTR YYDDIYFDSD SEDEDRAVQV 130 140 150 160 170 180 TKKKKKKQHK IPTNDELLYD PEKDNRDQAW VDAQRRGYHG LGPQRSRQQQ PVPNSDAVLN 190 200 210 220 230 240 CPACMTTLCL DCQRHESYKT QYRAMFVMNC SINKEEVLRY KASENRKKRR VHKKMRSNRE 250 260 270 280 DAAEKAETDV EEIYHPVMCT ECSTEVAVYD KDEVFHFFNV LASHS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Defining a holoprosencephaly locus on human chromosome 14q13 and characterization of potential candidate genes." Kamnasaran D., Chen C.-P., Devriendt K., Mehta L., Cox D.W. Genomics 85:608-621(2005) [PubMed: 15820313] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Fetal brain.
[2]	"EAPP, a novel E2F binding protein that modulates E2F-dependent transcription." Novy M., Pohn R., Andorfer P., Novy-Weiland T., Galos B., Schwarzmayr L., Rotheneder H. Mol. Biol. Cell 16:2181-2190(2005) [PubMed: 15716352] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, PHOSPHORYLATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH E2F1; E2F2 AND E2F3, VARIANT GLU-168.
[3]	"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. , Gu J., Chen S.-J., Chen Z. Genome Res. 10:1546-1560(2000) [PubMed: 11042152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed: 12508121] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLU-168. Tissue: Cervix.
[7]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17; SER-24; SER-25; SER-26; THR-37; THR-50; SER-53; SER-55; SER-56; SER-57; SER-109 AND SER-111, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-109 AND SER-111, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-109 AND SER-111, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY157300 mRNA. Translation: AAO17041.1. Frameshift. AY157301 mRNA. Translation: AAO17042.1. Frameshift. AY869694 mRNA. Translation: AAX63200.1. AF217512 mRNA. Translation: AAF67623.1. Frameshift. AK000585 mRNA. Translation: BAA91271.1. AL445363 Genomic DNA. No translation available. BC001245 mRNA. Translation: AAH01245.1.
IPI	IPI00306299.
RefSeq	NP_060923.2. NM_018453.3.
UniGene	Hs.433269.
3D structure databases
ProteinModelPortal	Q56P03.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q56P03. 5 interactions.
MINT	MINT-2874613.
STRING	Q56P03.
PTM databases
PhosphoSite	Q56P03.
Polymorphism databases
DMDM	296439474.
Proteomic databases
PRIDE	Q56P03.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000250454; ENSP00000250454; ENSG00000129518.
GeneID	55837.
KEGG	hsa:55837.
UCSC	uc001wsd.1. human.
Organism-specific databases
CTD	55837.
GeneCards	GC14M034985.
HGNC	HGNC:19312. EAPP.
HPA	HPA002916.
MIM	609486. gene.
neXtProt	NX_Q56P03.
PharmGKB	PA162384208.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG09650.
GeneTree	ENSGT00390000001332.
HOGENOM	HBG717164.
HOVERGEN	HBG054593.
InParanoid	Q56P03.
OMA	QDDYDPY.
OrthoDB	EOG4K9BCZ.
PhylomeDB	Q56P03.
Gene expression databases
ArrayExpress	Q56P03.
Bgee	Q56P03.
CleanEx	HS_EAPP.
Genevestigator	Q56P03.
GermOnline	ENSG00000129518. Homo sapiens.
Family and domain databases
InterPro	IPR019370. E2F-assoc_phosphoprotein. [Graphical view]
Pfam	PF10238. Eapp_C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	61061.
SOURCE	Search...

Entry information

Entry name

EAPP_HUMAN

Accession

Primary (citable) accession number: Q56P03
Secondary accession number(s): Q9BVF4, Q9NWV5, Q9NZ86

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	May 18, 2010
Last modified:	January 25, 2012
This is version 65 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.