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Protein

Eukaryotic translation initiation factor 3 subunit H

Gene

EIF3H

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis

Enzyme and pathway databases

ReactomeiR-BTA-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-BTA-72649. Translation initiation complex formation.
R-BTA-72689. Formation of a pool of free 40S subunits.
R-BTA-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-BTA-72702. Ribosomal scanning and start codon recognition.
R-BTA-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Protein family/group databases

MEROPSiM67.971.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit HUniRule annotation
Short name:
eIF3hUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 3UniRule annotation
eIF-3-gamma
eIF3 p40 subunitUniRule annotation
Gene namesi
Name:EIF3HUniRule annotation
Synonyms:EIF3S3UniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 14

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002310021 – 352Eukaryotic translation initiation factor 3 subunit HAdd BLAST352

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei3PhosphoserineBy similarity1
Modified residuei183PhosphoserineUniRule annotationBy similarity1
Cross-linki303Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ56JZ5.
PRIDEiQ56JZ5.

Expressioni

Gene expression databases

BgeeiENSBTAG00000014032.

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with RNF139; the interaction leads to protein translation inhibitions in a ubiquitination-dependent manner.UniRule annotation

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000018645.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 173MPNPROSITE-ProRule annotationAdd BLAST135

Sequence similaritiesi

Belongs to the eIF-3 subunit H family.UniRule annotation

Phylogenomic databases

eggNOGiKOG1560. Eukaryota.
ENOG410XSR9. LUCA.
GeneTreeiENSGT00730000111042.
HOGENOMiHOG000248787.
HOVERGENiHBG000883.
InParanoidiQ56JZ5.
KOiK03247.
OMAiKFNRYQQ.
OrthoDBiEOG091G0E5O.
TreeFamiTF101504.

Family and domain databases

CDDicd08065. MPN_eIF3h. 1 hit.
HAMAPiMF_03007. eIF3h. 1 hit.
InterProiView protein in InterPro
IPR027524. eIF3h.
IPR000555. JAMM/MPN+_dom.
PANTHERiPTHR10410:SF3. PTHR10410:SF3. 1 hit.
PfamiView protein in Pfam
PF01398. JAB. 1 hit.
SMARTiView protein in SMART
SM00232. JAB_MPN. 1 hit.
PROSITEiView protein in PROSITE
PS50249. MPN. 1 hit.

Sequencei

Sequence statusi: Complete.

Q56JZ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASRKEGTGS SATSSSSTTG AAGKGKGKGG SGDSAVKQVQ IDGLVVLKII
60 70 80 90 100
KHYQEEGQGT EVVQGVLLGL VVEDRLEITN CFPFPQHTED DADFDEVQYQ
110 120 130 140 150
MEMMRSLRHV NIDHLHVGWY QSTYYGSFVT RALLDSQFSY QHAIEESVVL
160 170 180 190 200
IYDPIKTAQG SLSLKAYRLT PKLMEVCKEK DFSPEALKKG NITFEHMFEE
210 220 230 240 250
VPIVIKNSHL INVLMWELEK KSAVADKHEL LSLASSNHLG KTLQLLMDRV
260 270 280 290 300
DEMSQDIVKY NTYMRNTSKQ QQQKHQYQQR RQQENMQRQS RGEPPLPEED
310 320 330 340 350
LSKLFKPHQP PARMDSLLIA GQINTYCQNI KEFTAQNLGK LFMAQALQEY

NS
Length:352
Mass (Da):39,906
Last modified:May 10, 2005 - v1
Checksum:i29D678DEC880CE8E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY911332 mRNA. Translation: AAW82100.1.
BC102096 mRNA. Translation: AAI02097.1.
RefSeqiNP_001020493.1. NM_001025322.2.
UniGeneiBt.16723.

Genome annotation databases

EnsembliENSBTAT00000018645; ENSBTAP00000018645; ENSBTAG00000014032.
GeneIDi506873.
KEGGibta:506873.

Similar proteinsi

Entry informationi

Entry nameiEIF3H_BOVIN
AccessioniPrimary (citable) accession number: Q56JZ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: May 10, 2005
Last modified: October 25, 2017
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. SIMILARITY comments
    Index of protein domains and families