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Q56JV6

- TWF1_BOVIN

UniProt

Q56JV6 - TWF1_BOVIN

Protein

Twinfilin-1

Gene

TWF1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (10 May 2005)
      Previous versions | rss
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    Functioni

    Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles By similarity.By similarity

    GO - Molecular functioni

    1. ATP binding Source: Ensembl
    2. phosphatidylinositol-4,5-bisphosphate binding Source: Ensembl
    3. protein tyrosine kinase activity Source: Ensembl

    GO - Biological processi

    1. barbed-end actin filament capping Source: Ensembl
    2. regulation of actin phosphorylation Source: Ensembl
    3. sequestering of actin monomers Source: Ensembl

    Keywords - Ligandi

    Actin-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Twinfilin-1
    Gene namesi
    Name:TWF1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 5

    Subcellular locationi

    Cytoplasm. Cytoplasmcytoskeleton By similarity
    Note: Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts By similarity.By similarity

    GO - Cellular componenti

    1. actin cytoskeleton Source: Ensembl
    2. cell-cell junction Source: Ensembl
    3. filopodium Source: Ensembl
    4. myofibril Source: Ensembl
    5. perinuclear region of cytoplasm Source: Ensembl
    6. ruffle membrane Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 350349Twinfilin-1PRO_0000232404Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei143 – 1431PhosphoserineBy similarity
    Modified residuei309 – 3091PhosphotyrosineBy similarity
    Modified residuei349 – 3491PhosphothreonineBy similarity

    Post-translational modificationi

    Phosphorylated on serine and threonine residues.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PRIDEiQ56JV6.

    Interactioni

    Subunit structurei

    Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF2 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down-regulated By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000027889.

    Structurei

    3D structure databases

    ProteinModelPortaliQ56JV6.
    SMRiQ56JV6. Positions 7-139, 161-313.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 139138ADF-H 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini177 – 313137ADF-H 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 2 ADF-H domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG263290.
    GeneTreeiENSGT00530000063868.
    HOGENOMiHOG000168296.
    HOVERGENiHBG000848.
    InParanoidiQ56JV6.
    KOiK08870.
    OMAiTVFIYSM.
    OrthoDBiEOG79GT6W.
    TreeFamiTF352598.

    Family and domain databases

    Gene3Di3.40.20.10. 2 hits.
    InterProiIPR002108. ADF-H.
    IPR029006. ADF-H/Gelsolin-like_dom.
    IPR028458. Twinfilin.
    [Graphical view]
    PANTHERiPTHR13759. PTHR13759. 1 hit.
    PfamiPF00241. Cofilin_ADF. 2 hits.
    [Graphical view]
    SMARTiSM00102. ADF. 2 hits.
    [Graphical view]
    PROSITEiPS51263. ADF_H. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q56JV6-1 [UniParc]FASTAAdd to Basket

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    MSHQTGIQAS EDVKDIFARA RNGKYRLLKI SIENEKLVIG SCRKPSDSWD    50
    QDYDSFVLPL LEDKQPCYVL FRLDSQNAQG YEWIFIAWSP DHSHVRQKML 100
    YAATRATLKK EFGGGHIKDE MFGTVKEDVS LHGYKKYLLS QSSPAPLTAA 150
    EEELRQIKIN EVQTDVSVDA KHQTLQGVAF PISQEAFQAL EKLNNRQLNY 200
    VQLEIDIKNE IIILANTINT ELKDLPKRIP KDAARYHFFL YKHSHEGDYL 250
    ESIVFIYSMP GYTCSIRERM LYSSCKSPLL EIVERQLQMD IIRKIEIDNG 300
    DELTADFLYE EVHPKQHAHK QSFAKPKGPS GKRGIRRIIR GPAETEATTE 350
    Length:350
    Mass (Da):40,249
    Last modified:May 10, 2005 - v1
    Checksum:iEFEFC1E0DC21E7A3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY911376 mRNA. Translation: AAW82139.1.
    RefSeqiNP_001020491.1. NM_001025320.2.
    UniGeneiBt.5944.

    Genome annotation databases

    EnsembliENSBTAT00000027889; ENSBTAP00000027889; ENSBTAG00000020938.
    GeneIDi506683.
    KEGGibta:506683.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Molecular embrace - Issue 73 of August 2006

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY911376 mRNA. Translation: AAW82139.1 .
    RefSeqi NP_001020491.1. NM_001025320.2.
    UniGenei Bt.5944.

    3D structure databases

    ProteinModelPortali Q56JV6.
    SMRi Q56JV6. Positions 7-139, 161-313.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000027889.

    Proteomic databases

    PRIDEi Q56JV6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000027889 ; ENSBTAP00000027889 ; ENSBTAG00000020938 .
    GeneIDi 506683.
    KEGGi bta:506683.

    Organism-specific databases

    CTDi 5756.

    Phylogenomic databases

    eggNOGi NOG263290.
    GeneTreei ENSGT00530000063868.
    HOGENOMi HOG000168296.
    HOVERGENi HBG000848.
    InParanoidi Q56JV6.
    KOi K08870.
    OMAi TVFIYSM.
    OrthoDBi EOG79GT6W.
    TreeFami TF352598.

    Miscellaneous databases

    NextBioi 20867714.

    Family and domain databases

    Gene3Di 3.40.20.10. 2 hits.
    InterProi IPR002108. ADF-H.
    IPR029006. ADF-H/Gelsolin-like_dom.
    IPR028458. Twinfilin.
    [Graphical view ]
    PANTHERi PTHR13759. PTHR13759. 1 hit.
    Pfami PF00241. Cofilin_ADF. 2 hits.
    [Graphical view ]
    SMARTi SM00102. ADF. 2 hits.
    [Graphical view ]
    PROSITEi PS51263. ADF_H. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of sequences obtained from constructed full-length bovine cDNA libraries."
      Yu J., Meng Y., Wang Z., Hansen C., Li C., Moore S.S.
      Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lymphoid epithelium.

    Entry informationi

    Entry nameiTWF1_BOVIN
    AccessioniPrimary (citable) accession number: Q56JV6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 4, 2006
    Last sequence update: May 10, 2005
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3