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Q56JV6 (TWF1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Twinfilin-1
Gene names
Name:TWF1
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length350 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles By similarity.

Subunit structure

Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF2 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down-regulated By similarity.

Subcellular location

Cytoplasm. Cytoplasmcytoskeleton By similarity. Note: Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts By similarity.

Post-translational modification

Phosphorylated on serine and threonine residues By similarity.

Sequence similarities

Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.

Contains 2 ADF-H domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 350349Twinfilin-1
PRO_0000232404

Regions

Domain2 – 139138ADF-H 1
Domain177 – 313137ADF-H 2

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue1431Phosphoserine By similarity
Modified residue3091Phosphotyrosine By similarity
Modified residue3491Phosphothreonine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q56JV6 [UniParc].

Last modified May 10, 2005. Version 1.
Checksum: EFEFC1E0DC21E7A3

FASTA35040,249
        10         20         30         40         50         60 
MSHQTGIQAS EDVKDIFARA RNGKYRLLKI SIENEKLVIG SCRKPSDSWD QDYDSFVLPL 

        70         80         90        100        110        120 
LEDKQPCYVL FRLDSQNAQG YEWIFIAWSP DHSHVRQKML YAATRATLKK EFGGGHIKDE 

       130        140        150        160        170        180 
MFGTVKEDVS LHGYKKYLLS QSSPAPLTAA EEELRQIKIN EVQTDVSVDA KHQTLQGVAF 

       190        200        210        220        230        240 
PISQEAFQAL EKLNNRQLNY VQLEIDIKNE IIILANTINT ELKDLPKRIP KDAARYHFFL 

       250        260        270        280        290        300 
YKHSHEGDYL ESIVFIYSMP GYTCSIRERM LYSSCKSPLL EIVERQLQMD IIRKIEIDNG 

       310        320        330        340        350 
DELTADFLYE EVHPKQHAHK QSFAKPKGPS GKRGIRRIIR GPAETEATTE 

« Hide

References

[1]"Analysis of sequences obtained from constructed full-length bovine cDNA libraries."
Yu J., Meng Y., Wang Z., Hansen C., Li C., Moore S.S.
Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lymphoid epithelium.

Web resources

Protein Spotlight

Molecular embrace - Issue 73 of August 2006

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY911376 mRNA. Translation: AAW82139.1.
RefSeqNP_001020491.1. NM_001025320.2.
UniGeneBt.5944.

3D structure databases

ProteinModelPortalQ56JV6.
SMRQ56JV6. Positions 7-139, 161-313.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000027889.

Proteomic databases

PRIDEQ56JV6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000027889; ENSBTAP00000027889; ENSBTAG00000020938.
GeneID506683.
KEGGbta:506683.

Organism-specific databases

CTD5756.

Phylogenomic databases

eggNOGNOG263290.
GeneTreeENSGT00530000063868.
HOGENOMHOG000168296.
HOVERGENHBG000848.
InParanoidQ56JV6.
KOK08870.
OMATVFIYSM.
OrthoDBEOG79GT6W.
TreeFamTF352598.

Family and domain databases

InterProIPR002108. ADF-H.
IPR028458. Twinfilin.
[Graphical view]
PANTHERPTHR13759. PTHR13759. 1 hit.
PfamPF00241. Cofilin_ADF. 2 hits.
[Graphical view]
SMARTSM00102. ADF. 2 hits.
[Graphical view]
PROSITEPS51263. ADF_H. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio20867714.

Entry information

Entry nameTWF1_BOVIN
AccessionPrimary (citable) accession number: Q56JV6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: May 10, 2005
Last modified: April 16, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries