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Q569Z6

- TR150_MOUSE

UniProt

Q569Z6 - TR150_MOUSE

Protein

Thyroid hormone receptor-associated protein 3

Gene

Thrap3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (10 May 2005)
      Previous versions | rss
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    Functioni

    Involved in pre-mRNA splicing. Remains associated with spliced mRNA after splicing which probably involves interactions with the exon junction complex (EJC). Can trigger mRNA decay which seems to be independent of nonsense-mediated decay involving premature stop codons (PTC) recognition. May be involved in nuclear mRNA decay. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45 is proposed to sequester phosphorylated SFPQ from PTPRC/CD45 pre-mRNA in resting T-cells. Involved in cyclin-D1/CCND1 mRNA stability probably by acting as component of the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. Involved in response to DNA damage. Is excluced from DNA damage sites in a manner that parallels transcription inhibition; the function may involve the SNARP complex. Initially thought to play a role in transcriptional coactivation through its association with the TRAP complex; however, it is not regarded as a stable Mediator complex subunit. Cooperatively with HELZ2, enhances the transcriptional activation mediated by PPARG, maybe through the stabilization of the PPARG binding to DNA in presence of ligand. May play a role in the terminal stage of adipocyte differentiation.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi549 – 5568ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. phosphoprotein binding Source: UniProtKB
    3. receptor activity Source: Ensembl
    4. RNA polymerase II transcription cofactor activity Source: Ensembl
    5. transcription coactivator activity Source: Ensembl

    GO - Biological processi

    1. androgen receptor signaling pathway Source: Ensembl
    2. mRNA processing Source: UniProtKB-KW
    3. mRNA stabilization Source: UniProtKB
    4. nuclear-transcribed mRNA catabolic process Source: UniProtKB
    5. positive regulation of mRNA splicing, via spliceosome Source: UniProtKB
    6. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
    7. regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
    8. RNA splicing Source: UniProtKB-KW
    9. transcription from RNA polymerase II promoter Source: MGI
    10. transcription initiation from RNA polymerase II promoter Source: Ensembl

    Keywords - Molecular functioni

    Activator, Receptor

    Keywords - Biological processi

    mRNA processing, mRNA splicing, Transcription, Transcription regulation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thyroid hormone receptor-associated protein 3
    Alternative name(s):
    Thyroid hormone receptor-associated protein complex 150 kDa component
    Short name:
    Trap150
    Gene namesi
    Name:Thrap3
    Synonyms:Trap150
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:2442637. Thrap3.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. exon-exon junction complex Source: Ensembl
    2. mediator complex Source: MGI
    3. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 951950Thyroid hormone receptor-associated protein 3PRO_0000235980Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei17 – 171Dimethylated arginineBy similarity
    Modified residuei221 – 2211N6-acetyllysineBy similarity
    Modified residuei233 – 2331PhosphoserineBy similarity
    Modified residuei238 – 2381PhosphoserineBy similarity
    Modified residuei240 – 2401PhosphoserineBy similarity
    Modified residuei243 – 2431Phosphoserine3 Publications
    Modified residuei248 – 2481Phosphoserine1 Publication
    Modified residuei253 – 2531Phosphoserine1 Publication
    Modified residuei257 – 2571PhosphoserineBy similarity
    Modified residuei315 – 3151Phosphoserine1 Publication
    Modified residuei320 – 3201Phosphoserine1 Publication
    Modified residuei339 – 3391PhosphoserineBy similarity
    Modified residuei346 – 3461N6-acetyllysine1 Publication
    Modified residuei379 – 3791Phosphoserine2 Publications
    Modified residuei403 – 4031PhosphoserineBy similarity
    Modified residuei405 – 4051PhosphoserineBy similarity
    Modified residuei452 – 4521N6-acetyllysine1 Publication
    Modified residuei468 – 4681N6-acetyllysine1 Publication
    Modified residuei476 – 4761N6-acetyllysine1 Publication
    Modified residuei516 – 5161N6-acetyllysineBy similarity
    Modified residuei524 – 5241N6-acetyllysine1 Publication
    Modified residuei532 – 5321PhosphoserineBy similarity
    Modified residuei555 – 5551N6-acetyllysine1 Publication
    Modified residuei572 – 5721Phosphoserine3 Publications
    Modified residuei619 – 6191PhosphoserineBy similarity
    Modified residuei669 – 6691PhosphoserineBy similarity
    Modified residuei679 – 6791Phosphoserine3 Publications
    Modified residuei695 – 6951PhosphoserineBy similarity
    Modified residuei808 – 8081N6-acetyllysineBy similarity
    Modified residuei870 – 8701PhosphothreonineBy similarity
    Modified residuei924 – 9241Phosphoserine2 Publications
    Modified residuei935 – 9351Phosphoserine2 Publications

    Keywords - PTMi

    Acetylation, Methylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ569Z6.
    PaxDbiQ569Z6.
    PRIDEiQ569Z6.

    PTM databases

    PhosphoSiteiQ569Z6.

    Expressioni

    Gene expression databases

    ArrayExpressiQ569Z6.
    BgeeiQ569Z6.
    CleanExiMM_THRAP3.
    GenevestigatoriQ569Z6.

    Interactioni

    Subunit structurei

    Associated with the large multiprotein complex TRAP (Mediator complex-like). Interacts with SFPQ; the interaction is dependent on SFPQ phosphorylation at 'Thr-679' and inhibits binding of SFPQ to an ESS1 exonic splicing silencer element-containing RNA. Interacts with NXF1. Component of the SNARP complex which consists at least of SNIP1, SNW1, THRAP3, BCLAF1 and PNN. Associated with spliced mRNP complexes. Interacts with HELZ2 and PPARG.1 Publication

    Protein-protein interaction databases

    BioGridi231014. 3 interactions.
    IntActiQ569Z6. 4 interactions.
    STRINGi10090.ENSMUSP00000079722.

    Structurei

    3D structure databases

    ProteinModelPortaliQ569Z6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 190189Required for mRNA splicing activationBy similarityAdd
    BLAST
    Regioni359 – 951593Required for mRNA decay activityBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi7 – 339333Ser-richAdd
    BLAST
    Compositional biasi12 – 161150Arg-richAdd
    BLAST

    Phylogenomic databases

    eggNOGiNOG43306.
    GeneTreeiENSGT00530000063211.
    HOGENOMiHOG000231570.
    HOVERGENiHBG054554.
    InParanoidiQ569Z6.
    KOiK13112.
    OMAiRSTEKTE.
    OrthoDBiEOG7RBZ7X.
    PhylomeDBiQ569Z6.
    TreeFamiTF335939.

    Family and domain databases

    InterProiIPR026667. THRAP3.
    IPR029199. THRAP3_BCLAF1.
    [Graphical view]
    PANTHERiPTHR15268:SF16. PTHR15268:SF16. 1 hit.
    PfamiPF15440. THRAP3_BCLAF1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q569Z6-1 [UniParc]FASTAAdd to Basket

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    MSKTNKSKSG SRSSRSRSAS RSRSRSFSKS RSRSRSVSRS RKRRLSSRSR    50
    SRSYSPAHNR ERNHPRVYQN RDFRGHNRGY RRPYYFRGRN RGFYPWGQYN 100
    RGGYGNYRSN WQNYRQAYSP RRGRSRSRSP KRRSPSPRSR SHSRNSDKSS 150
    SDRSRRSSSS RSSSNHSRVE SSKRKSTKEK KSSSKDSRPS QAAGDNQGDE 200
    AKEQTFSGGT SQDIKGSESS KPWPDATTYG AGSASRASVS DLSPRERSPA 250
    LKSPLQSVVV RRRSPRPSPV PKPSPPLSNA SQMGSSMSGG AGYQSGAHQG 300
    QFDHGSGSLS PSKKSPVGKS PPATGSAYGS SQKEESAASG GAAYSKRYLE 350
    EQKTENGKDK EQKQTNADKE KLKEKGGFSD ADVKMKSDPF APKTDSEKPF 400
    RGSQSPKRYK LRDDFEKKMA DFHKEELDEH DKDKSKGRKE PEFDDEPKFM 450
    SKVIAGASKN QEEEKSGKWE SLHTGKEKQR KAEEMEDEPF TERSRKEERG 500
    GSKRSESGHR GFVPEKNFRV TAYKAVQEKS SSPPPRKTSE SRDKLGSKGD 550
    FSSGKSSFSI TREAQVNVRM DSFDEDLARP SGLLAQERKL CRDLVHSNKK 600
    EQEFRSIFQH IQSAQSQRSP SELFAQHIVT IVHHVKEHHF GSSGMTLHER 650
    FTKYLKRGNE QEAAKNKKSP EIHRRIDISP STFRKHGLTH EELKSPREPG 700
    YKAEGKYKDD PVDLRLDIER RKKHKERDLK RGKSRESVDS RDSSHSRERS 750
    TEKTEKTHKG SKKQKKHRRA RDRSRSSSSS SQSSHSYKAE EYPEEAEERE 800
    ESTSGFDKSR LGTKDFVGPN ERGGRARGTF QFRARGRGWG RGNYSGNNNN 850
    NSNNDFQKRS REEEWDPEYT PKSKKYYLHD DREGEGSDKW MGRGRGRGAF 900
    PRGRGRFMFR KSSTSPKWAH DKFSGEEGEI EDDESGTENR EEKDSLQPSA 950
    E 951
    Length:951
    Mass (Da):108,178
    Last modified:May 10, 2005 - v1
    Checksum:i40DF483027363B4E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC092239 mRNA. Translation: AAH92239.1.
    CCDSiCCDS18645.1.
    RefSeqiNP_666265.3. NM_146153.3.
    XP_006503104.1. XM_006503041.1.
    UniGeneiMm.236211.
    Mm.29813.

    Genome annotation databases

    EnsembliENSMUST00000080919; ENSMUSP00000079722; ENSMUSG00000043962.
    GeneIDi230753.
    KEGGimmu:230753.
    UCSCiuc008usv.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC092239 mRNA. Translation: AAH92239.1 .
    CCDSi CCDS18645.1.
    RefSeqi NP_666265.3. NM_146153.3.
    XP_006503104.1. XM_006503041.1.
    UniGenei Mm.236211.
    Mm.29813.

    3D structure databases

    ProteinModelPortali Q569Z6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 231014. 3 interactions.
    IntActi Q569Z6. 4 interactions.
    STRINGi 10090.ENSMUSP00000079722.

    PTM databases

    PhosphoSitei Q569Z6.

    Proteomic databases

    MaxQBi Q569Z6.
    PaxDbi Q569Z6.
    PRIDEi Q569Z6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000080919 ; ENSMUSP00000079722 ; ENSMUSG00000043962 .
    GeneIDi 230753.
    KEGGi mmu:230753.
    UCSCi uc008usv.1. mouse.

    Organism-specific databases

    CTDi 9967.
    MGIi MGI:2442637. Thrap3.

    Phylogenomic databases

    eggNOGi NOG43306.
    GeneTreei ENSGT00530000063211.
    HOGENOMi HOG000231570.
    HOVERGENi HBG054554.
    InParanoidi Q569Z6.
    KOi K13112.
    OMAi RSTEKTE.
    OrthoDBi EOG7RBZ7X.
    PhylomeDBi Q569Z6.
    TreeFami TF335939.

    Miscellaneous databases

    ChiTaRSi THRAP3. mouse.
    NextBioi 380111.
    PROi Q569Z6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q569Z6.
    Bgeei Q569Z6.
    CleanExi MM_THRAP3.
    Genevestigatori Q569Z6.

    Family and domain databases

    InterProi IPR026667. THRAP3.
    IPR029199. THRAP3_BCLAF1.
    [Graphical view ]
    PANTHERi PTHR15268:SF16. PTHR15268:SF16. 1 hit.
    Pfami PF15440. THRAP3_BCLAF1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Head.
    2. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-572, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-243; SER-315; SER-320; SER-379; SER-924 AND SER-935, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    4. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
      Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
      J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-243 AND SER-679, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    5. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-379; SER-572; SER-679; SER-924 AND SER-935, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-243; SER-248; SER-253; SER-572 AND SER-679, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.
    7. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-346; LYS-452; LYS-468; LYS-476; LYS-524 AND LYS-555, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.
    8. Cited for: FUNCTION, INTERACTION WITH HELZ2 AND PPARG, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiTR150_MOUSE
    AccessioniPrimary (citable) accession number: Q569Z6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2006
    Last sequence update: May 10, 2005
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

    External Data

    Dasty 3