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Protein

Probable ATP-dependent RNA helicase DDX46

Gene

Ddx46

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an essential role in splicing, either prior to, or during A complex formation.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi416 – 4238ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DDX46 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 46
Gene namesi
Name:Ddx46
Synonyms:Kiaa0801
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1920895. Ddx46.

Subcellular locationi

  • Nucleus speckle By similarity
  • NucleusCajal body By similarity
  • Membrane By similarity; Lipid-anchor By similarity

  • Note: Present in Cajal bodies (CBs) and nuclear speckles.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 10321031Probable ATP-dependent RNA helicase DDX46PRO_0000055122Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Cross-linki186 – 186Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei199 – 1991PhosphoserineBy similarity
Modified residuei263 – 2631N6-acetyllysineBy similarity
Modified residuei294 – 2941PhosphotyrosineCombined sources
Modified residuei295 – 2951PhosphoserineCombined sources
Modified residuei296 – 2961PhosphoserineCombined sources
Modified residuei776 – 7761N6-acetyllysineBy similarity
Modified residuei804 – 8041PhosphoserineCombined sources
Modified residuei904 – 9041N6-acetyllysineCombined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Lipoprotein, Myristate, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ569Z5.
MaxQBiQ569Z5.
PaxDbiQ569Z5.
PRIDEiQ569Z5.

PTM databases

iPTMnetiQ569Z5.
PhosphoSiteiQ569Z5.

Expressioni

Gene expression databases

BgeeiQ569Z5.
CleanExiMM_DDX46.
ExpressionAtlasiQ569Z5. baseline and differential.
GenevisibleiQ569Z5. MM.

Interactioni

Subunit structurei

Integral component of the 17S U2 snRNP.By similarity

Protein-protein interaction databases

IntActiQ569Z5. 1 interaction.
MINTiMINT-4118429.
STRINGi10090.ENSMUSP00000133245.

Structurei

3D structure databases

ProteinModelPortaliQ569Z5.
SMRiQ569Z5. Positions 327-738.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini403 – 581179Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini592 – 753162Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili152 – 19746Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi372 – 40029Q motifAdd
BLAST
Motifi529 – 5324DEAD box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi3 – 103101Arg-richAdd
BLAST
Compositional biasi202 – 2098Poly-Asp
Compositional biasi297 – 3004Poly-Glu

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0334. Eukaryota.
ENOG410XSQV. LUCA.
GeneTreeiENSGT00840000129934.
HOGENOMiHOG000007229.
HOVERGENiHBG081426.
InParanoidiQ569Z5.
KOiK12811.
OMAiQEGGQSE.
OrthoDBiEOG718KBG.
PhylomeDBiQ569Z5.
TreeFamiTF354236.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q569Z5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRESRHYRK RSASRGRSGS RSRSRSPSDK RSKRGDDRRS RSRDRDRRRE
60 70 80 90 100
RSRSRDKRRS RSRDRKRLRR SRSRERDRSR ERRRSRSRDR RRSRSRSRGR
110 120 130 140 150
RSRSSSPGSK TKKTENRSRS KEKAEGGDSS KEKKKDKDDK EDEKEKDAGN
160 170 180 190 200
FDQNKLEEEM RKRKERVEKW REEQRKKAME NIGELKKEIE EMKQGKKWSL
210 220 230 240 250
EDDDDDEDDP AEAEKEGTEM EDEELDPLDA YMEEVKEEVK KFNMRSVKGG
260 270 280 290 300
AGNEKKSGPT VTKVVTVVTT KKAVVDADKK KGELMENDQD AMEYSSEEEE
310 320 330 340 350
VDLQTALTGY QTKQRKLLEP VDHGKIEYEP FRKNFYVEVP ELAKMSQEEV
360 370 380 390 400
NVFRLEMEGI TVKGKGCPKP IKSWVQCGIS MKILNSLKKH GYEKPTPIQT
410 420 430 440 450
QAIPAIMSGR DLIGIAKTGS GKTIAFLLPM FRHIMDQRSL EEGEGPIAVI
460 470 480 490 500
MTPTRELALQ ITKECKKFSK TLGLRVVCVY GGTGISEQIA ELKRGAEIIV
510 520 530 540 550
CTPGRMIDML AANSGRVTNL RRVTYVVLDE ADRMFDMGFE PQVMRIVDNV
560 570 580 590 600
RPDRQTVMFS ATFPRAMEAL ARRILSKPIE VQVGGRSVVC SDVEQQVIVI
610 620 630 640 650
EEEKKFLKLL ELLGHYQESG SVIIFVDKQE HADGLLKDLM RASYPCMSLH
660 670 680 690 700
GGIDQYDRDS IINDFKNGTC KLLVATSVAA RGLDVKHLIL VVNYSCPNHY
710 720 730 740 750
EDYVHRAGRT GRAGNKGYAY TFITEDQARY AGDIIKALEL SGTAVPPDLE
760 770 780 790 800
KLWSDFKDQQ KAEGKIIKKS SGFSGKGFKF DETEQALANE RKKLQKAALG
810 820 830 840 850
LQDSDDEDAA VDIDEQIESM FNSKKRVKDM AAPGTSSVPA PTAGNAEKLE
860 870 880 890 900
IAKRLALRIN AQKNLGIESQ VDVMQQATNA ILRGGTILAP TVSAKTIAEQ
910 920 930 940 950
LAEKINAKLN YVPLEKQEEE RQEGGQSESF KRYEEELEIN DFPQTARWKV
960 970 980 990 1000
TSKEALQRIS EYSEAAITIR GTYFPPGKEP KEGERKIYLA IESANELAVQ
1010 1020 1030
KAKAEITRLI KEELIRLQNS YQPTNKGRYK VL
Length:1,032
Mass (Da):117,448
Last modified:January 10, 2006 - v2
Checksum:iF690CE9973A079AA
GO
Isoform 2 (identifier: Q569Z5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     516-544: RVTNLRRVTYVVLDEADRMFDMGFEPQVM → KSRVFYYLFSLLFVLDMIFVEADL

Note: No experimental confirmation available.
Show »
Length:1,027
Mass (Da):116,889
Checksum:i755C384DE528A758
GO

Sequence cautioni

The sequence AAH26492.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH92240.1 differs from that shown. Reason: Erroneous termination at position 1028. Translated as Arg.Curated
The sequence BAC98030.2 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei516 – 54429RVTNL…EPQVM → KSRVFYYLFSLLFVLDMIFV EADL in isoform 2. 1 PublicationVSP_016859Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129220 Transcribed RNA. Translation: BAC98030.2. Different initiation.
BC026492 mRNA. Translation: AAH26492.1. Different initiation.
BC092240 mRNA. Translation: AAH92240.1. Sequence problems.
CCDSiCCDS70464.1. [Q569Z5-1]
RefSeqiNP_001268984.1. NM_001282055.1. [Q569Z5-1]
UniGeneiMm.202725.

Genome annotation databases

EnsembliENSMUST00000172272; ENSMUSP00000133245; ENSMUSG00000021500. [Q569Z5-1]
GeneIDi212880.
KEGGimmu:212880.
UCSCiuc033gma.1. mouse. [Q569Z5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK129220 Transcribed RNA. Translation: BAC98030.2. Different initiation.
BC026492 mRNA. Translation: AAH26492.1. Different initiation.
BC092240 mRNA. Translation: AAH92240.1. Sequence problems.
CCDSiCCDS70464.1. [Q569Z5-1]
RefSeqiNP_001268984.1. NM_001282055.1. [Q569Z5-1]
UniGeneiMm.202725.

3D structure databases

ProteinModelPortaliQ569Z5.
SMRiQ569Z5. Positions 327-738.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ569Z5. 1 interaction.
MINTiMINT-4118429.
STRINGi10090.ENSMUSP00000133245.

PTM databases

iPTMnetiQ569Z5.
PhosphoSiteiQ569Z5.

Proteomic databases

EPDiQ569Z5.
MaxQBiQ569Z5.
PaxDbiQ569Z5.
PRIDEiQ569Z5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000172272; ENSMUSP00000133245; ENSMUSG00000021500. [Q569Z5-1]
GeneIDi212880.
KEGGimmu:212880.
UCSCiuc033gma.1. mouse. [Q569Z5-1]

Organism-specific databases

CTDi9879.
MGIiMGI:1920895. Ddx46.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0334. Eukaryota.
ENOG410XSQV. LUCA.
GeneTreeiENSGT00840000129934.
HOGENOMiHOG000007229.
HOVERGENiHBG081426.
InParanoidiQ569Z5.
KOiK12811.
OMAiQEGGQSE.
OrthoDBiEOG718KBG.
PhylomeDBiQ569Z5.
TreeFamiTF354236.

Miscellaneous databases

ChiTaRSiDdx46. mouse.
NextBioi373723.
PROiQ569Z5.
SOURCEiSearch...

Gene expression databases

BgeeiQ569Z5.
CleanExiMM_DDX46.
ExpressionAtlasiQ569Z5. baseline and differential.
GenevisibleiQ569Z5. MM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Embryonic tail.
  2. Okazaki N., Kikuno R., Nagase T., Ohara O., Koga H.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Eye and Head.
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-804, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-294; SER-295; SER-296 AND SER-804, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney, Pancreas, Spleen and Testis.
  6. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-904, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiDDX46_MOUSE
AccessioniPrimary (citable) accession number: Q569Z5
Secondary accession number(s): Q6ZQ42, Q8R0R6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: January 10, 2006
Last modified: May 11, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.