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Q569L8

- CENPJ_MOUSE

UniProt

Q569L8 - CENPJ_MOUSE

Protein

Centromere protein J

Gene

Cenpj

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Plays an important role in cell division and centrosome function by participating in centriole duplication. Inhibits microtubule nucleation from the centrosome By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: MGI

    GO - Biological processi

    1. centriole replication Source: Ensembl
    2. microtubule polymerization Source: Ensembl
    3. regulation of centriole replication Source: UniProtKB

    Enzyme and pathway databases

    ReactomeiREACT_196635. Regulation of PLK1 Activity at G2/M Transition.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Centromere protein J
    Short name:
    CENP-J
    Gene namesi
    Name:Cenpj
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:2684927. Cenpj.

    Subcellular locationi

    Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole By similarity
    Note: Localized within the center of microtubule asters. During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles By similarity.By similarity

    GO - Cellular componenti

    1. centriole Source: UniProtKB
    2. microtubule Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13441344Centromere protein JPRO_0000286053Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei577 – 5771Phosphoserine; by PLK2By similarity
    Modified residuei583 – 5831Phosphoserine; by PLK2By similarity
    Modified residuei748 – 7481PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylation at Ser-577 and Ser-583 by PLK2 is required for procentriole formation and centriole elongation. Phosphorylation by PLK2 oscillates during the cell cycle: it increases at G1/S transition and decreases during the exit from mitosis. Phosphorylation at Ser-583 is also mediated by PLK4 but is not a critical step in PLK4 function in procentriole assembly.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ569L8.
    PRIDEiQ569L8.

    PTM databases

    PhosphoSiteiQ569L8.

    Expressioni

    Gene expression databases

    BgeeiQ569L8.
    CleanExiMM_CENPJ.
    GenevestigatoriQ569L8.

    Interactioni

    Subunit structurei

    Part of a ternary complex composed of SASS6, CENPJ and CEP350. Associated with the gamma-tubulin complex. Interacts with the head domain of EPB41. Interacts with LYST By similarity. Interacts with CEP152 (via C-terminus) By similarity.By similarity

    Protein-protein interaction databases

    BioGridi230108. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ569L8.
    SMRiQ569L8. Positions 1173-1327.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the TCP10 family.Curated

    Phylogenomic databases

    eggNOGiNOG83204.
    GeneTreeiENSGT00530000063927.
    HOGENOMiHOG000111541.
    HOVERGENiHBG050894.
    InParanoidiQ569L8.
    KOiK11502.
    OMAiDSPQVCD.
    OrthoDBiEOG77DJ60.
    TreeFamiTF343156.

    Family and domain databases

    InterProiIPR009852. Tcp10_C_dom.
    IPR026581. TCP10_fam.
    [Graphical view]
    PANTHERiPTHR10331. PTHR10331. 1 hit.
    PfamiPF07202. Tcp10_C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q569L8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFLMPTSSEL NSGQNFLTQW MTSPSRAGVI LNRGFPILEA DDKQAATNVS     50
    TSFPAKATHF SNSFSISSEE DSFHEEQKLE AGGPYKPWSE NPEAPPVFPS 100
    VRKEPIASRQ DAPGCQEDNN NDLTPHLESE FKEVANKNPL FKKLEQLKEI 150
    QQKKQEQLKR QQLEQLQRLM EEQEKLLTMV SAQHAFPGTL LPDDQSQKHR 200
    SPGDLTLPPH SYSNPTQENS CASNVLPDEQ SNFCRATQDS VLTSKNASDL 250
    FYESQYQEAH VKRNDLKEES PAHPSGEGAL PRWEKKMGRS QEGKDVNLQK 300
    CGDSSEVVNI DERPIKAAVR EKQQTFEDYL EEQIQLEERE LRQKQLQEAE 350
    GPLLAKTKPK QPFLKRGEGL ARFTNAKSKF QKGKESKLAS TQSPSEDQPG 400
    SKVDRQHLQR KTALINKDLC AETPTVKKDS KARPKAGFAS LRQKPKVTKT 450
    NMRESLSPPG LKVQTGKKRD GQFRHQVKGE RNAHASNKEN VPACIKPWDA 500
    GCKMWSKTQG RERLPLSTGP VGCVVSRSPI RETDRETESS LDFSLQKKLE 550
    IWEREKEKEN LELDEFLFLE RAADEISFSS NSSFVLRILE RDQQICDGHR 600
    LSSTPVKAVQ QREAQQADPR GQSNCSEIPR YGVAHENESE CEAMLLSWGS 650
    GSPDGLRELS CKRSMKAFQT STSEIQSQWD ARDDGVANSD SSTESEEQHD 700
    ITIKPSTEVG DRVFSNREDS PQVCDAKGPI RDTGAQEDKW RDADLDLSDK 750
    ECSSDESVIV ESLNNKVLEP LRLPSSQAGS KIDFDDERSW TDLEENPYEH 800
    GVIHREEAIY GTPQTQCHSK SEGCVLDKTI KRKIAPVKKG EDFKCDRRIS 850
    PPPPSDLMVK FFPSLKPKPK LDSHLENESK LNLSQDQPPE FMVCFIGDSV 900
    RSQVLREKVT ELESEIEKFK AENTSLAKLR IERESALEKL RKEIADFEQQ 950
    KARELARIEE YRKEETRKLQ KERKVFEKYT AAARTFPDKK EREEIQALKQ 1000
    QIADLQEDLK RKETKWSSTQ SRLRSQIEML VKENTDLREE IKVMERFRLD 1050
    AWKRAEAMEN SPKACQYMMA TKKDESMNSS FQFQKSHVSS GVQVEKYKKK 1100
    YLPAQGNLSR RIKSAPPRDL GSSDKGQAAL PREPLQQVNF PDLEYKNKEE 1150
    KEEEIQGEIS HPDGKVEKIY KNGRRVVLFP NGTRKEVSAD GKSVTVTFFN 1200
    GDVKQVMPDE RVVYYYAAAQ TTHTTYPEGL EVLHFSSGQI EKHFPDGRKE 1250
    ITFPDQTIKT LFADGQEESI FPDGTIVRVQ RDGNKIIEFN NGQRELHTAQ 1300
    FKRREYPDGT VKTVYANGHQ ETKYTSGRVR VKDKDGNVLM DTEM 1344
    Length:1,344
    Mass (Da):153,079
    Last modified:July 27, 2011 - v2
    Checksum:i0C7596B79161C2C6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti716 – 7161N → S in AAH92399. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC154837 Genomic DNA. No translation available.
    BC092399 mRNA. Translation: AAH92399.1.
    CCDSiCCDS27150.1.
    RefSeqiNP_001014996.2. NM_001014996.2.
    UniGeneiMm.212525.

    Genome annotation databases

    EnsembliENSMUST00000065302; ENSMUSP00000065949; ENSMUSG00000064128.
    GeneIDi219103.
    KEGGimmu:219103.
    UCSCiuc007ucb.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC154837 Genomic DNA. No translation available.
    BC092399 mRNA. Translation: AAH92399.1 .
    CCDSi CCDS27150.1.
    RefSeqi NP_001014996.2. NM_001014996.2.
    UniGenei Mm.212525.

    3D structure databases

    ProteinModelPortali Q569L8.
    SMRi Q569L8. Positions 1173-1327.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 230108. 1 interaction.

    PTM databases

    PhosphoSitei Q569L8.

    Proteomic databases

    PaxDbi Q569L8.
    PRIDEi Q569L8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000065302 ; ENSMUSP00000065949 ; ENSMUSG00000064128 .
    GeneIDi 219103.
    KEGGi mmu:219103.
    UCSCi uc007ucb.2. mouse.

    Organism-specific databases

    CTDi 55835.
    MGIi MGI:2684927. Cenpj.

    Phylogenomic databases

    eggNOGi NOG83204.
    GeneTreei ENSGT00530000063927.
    HOGENOMi HOG000111541.
    HOVERGENi HBG050894.
    InParanoidi Q569L8.
    KOi K11502.
    OMAi DSPQVCD.
    OrthoDBi EOG77DJ60.
    TreeFami TF343156.

    Enzyme and pathway databases

    Reactomei REACT_196635. Regulation of PLK1 Activity at G2/M Transition.

    Miscellaneous databases

    NextBioi 376568.
    PROi Q569L8.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q569L8.
    CleanExi MM_CENPJ.
    Genevestigatori Q569L8.

    Family and domain databases

    InterProi IPR009852. Tcp10_C_dom.
    IPR026581. TCP10_fam.
    [Graphical view ]
    PANTHERi PTHR10331. PTHR10331. 1 hit.
    Pfami PF07202. Tcp10_C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Eye.

    Entry informationi

    Entry nameiCENPJ_MOUSE
    AccessioniPrimary (citable) accession number: Q569L8
    Secondary accession number(s): E9QLP3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 2007
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 63 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3