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Q569C3

- UBP1_RAT

UniProt

Q569C3 - UBP1_RAT

Protein

Ubiquitin carboxyl-terminal hydrolase 1

Gene

Usp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (10 May 2005)
      Previous versions | rss
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    Functioni

    Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei90 – 901NucleophilePROSITE-ProRule annotation
    Active sitei593 – 5931Proton acceptorPROSITE-ProRule annotation
    Sitei670 – 6712Cleavage; by autolysisBy similarity

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: UniProtKB
    2. ubiquitin thiolesterase activity Source: UniProtKB

    GO - Biological processi

    1. DNA repair Source: UniProtKB-KW
    2. monoubiquitinated protein deubiquitination Source: UniProtKB
    3. protein deubiquitination Source: UniProtKB
    4. regulation of DNA repair Source: UniProtKB
    5. response to UV Source: UniProtKB
    6. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    DNA damage, DNA repair, Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC19.019.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 1 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 1
    Ubiquitin thioesterase 1
    Ubiquitin-specific-processing protease 1
    Gene namesi
    Name:Usp1Imported
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 5

    Organism-specific databases

    RGDi1306461. Usp1.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 784784Ubiquitin carboxyl-terminal hydrolase 1PRO_0000306288Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei42 – 421PhosphoserineBy similarity
    Modified residuei67 – 671PhosphoserineBy similarity
    Modified residuei475 – 4751PhosphoserineBy similarity

    Post-translational modificationi

    Autocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis.By similarity
    Ubiquitinated; leading to its subsequent proteasomal degradation.By similarity

    Keywords - PTMi

    Autocatalytic cleavage, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiQ569C3.

    PTM databases

    PhosphoSiteiQ569C3.

    Expressioni

    Gene expression databases

    GenevestigatoriQ569C3.

    Interactioni

    Subunit structurei

    Interacts with FANCD2 and PCNA. Interacts with WDR48 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000010935.

    Structurei

    3D structure databases

    ProteinModelPortaliQ569C3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini81 – 784704USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Sequence Analysis
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5077.
    GeneTreeiENSGT00650000093027.
    HOGENOMiHOG000154756.
    HOVERGENiHBG017288.
    InParanoidiQ569C3.
    KOiK11832.
    OMAiFAVVMHS.
    OrthoDBiEOG7JT6VZ.
    PhylomeDBiQ569C3.
    TreeFamiTF331057.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 2 hits.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q569C3-1 [UniParc]FASTAAdd to Basket

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    MPGVIPSESN GLSRGSPSKK NRLSLKFFQK KETKRALDFT DSQEDEEKAS    50
    EYRGSEIDQV VPAAQSSPVS CEKRENLLPF VGLNNLGNTC YLNSILQVLY 100
    FCPGFKAGVK HLFNIISRKK EALKDDSIQK DKGSCKEDPL ASYELICSLQ 150
    SLIISVEQLQ ASFLLNPEKY TDELATQPRR LLNTLRELNP MYEGYLQHDA 200
    QEVLQCILGN IQETCQLLKK EEIKNLTEFS SKVEEKSLQK EETGGISSTE 250
    TDSTRNLDDL KEQLPKGNWK RKSDGESGNM KKKVKLSRES QPLEENQRQT 300
    RSKRKATGDT LEASPKIIPK CVSENESAKP SQKKSKVKIN WLKPATKQPS 350
    ILSKFCSLGK ITTNQRSKGQ PKVNEGDLEE DLEKDGRDNT VNGSGPASPG 400
    SSVTPVDSSE AKSINKGAEQ IGFELVEKLF QGQLVLRTRC LECESLTERR 450
    EDFQDISVPV QEDELSKVEE SSEISPEPKT EMKTLRWAIS QFASVERIVG 500
    EDKYFCENCH HYTEAERSLL FDKMPEVITI HLKCFAASGL EFDCYGGGLS 550
    KINTPLLTPL KLSLEEWSTK PTNDSYGLFA VVMHSGITIS SGHYTASVKV 600
    TDLNSLELDK GNFVVDQMCE IGKPEPLNEE EARGTAENYD DEVSIRVGGN 650
    AQPSKVLNKK NVEGIGLLGG QKSKADYELC SKASNPEKVV GTPFTDSRNS 700
    ETNDTNGTQE SDRSKESSDQ TGINVSGLEN KISYVVQSLK EYEGKWLLFD 750
    DSEVKVTEEK DFLNSLSPST SPTSTPYLLF YKKL 784
    Length:784
    Mass (Da):87,329
    Last modified:May 10, 2005 - v1
    Checksum:i4CAFB369297DE7CC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC092574 mRNA. Translation: AAH92574.1.
    RefSeqiNP_001015015.1. NM_001015015.1.
    UniGeneiRn.155728.

    Genome annotation databases

    EnsembliENSRNOT00000010933; ENSRNOP00000010935; ENSRNOG00000007890.
    GeneIDi313387.
    KEGGirno:313387.
    UCSCiRGD:1306461. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC092574 mRNA. Translation: AAH92574.1 .
    RefSeqi NP_001015015.1. NM_001015015.1.
    UniGenei Rn.155728.

    3D structure databases

    ProteinModelPortali Q569C3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000010935.

    Protein family/group databases

    MEROPSi C19.019.

    PTM databases

    PhosphoSitei Q569C3.

    Proteomic databases

    PRIDEi Q569C3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000010933 ; ENSRNOP00000010935 ; ENSRNOG00000007890 .
    GeneIDi 313387.
    KEGGi rno:313387.
    UCSCi RGD:1306461. rat.

    Organism-specific databases

    CTDi 7398.
    RGDi 1306461. Usp1.

    Phylogenomic databases

    eggNOGi COG5077.
    GeneTreei ENSGT00650000093027.
    HOGENOMi HOG000154756.
    HOVERGENi HBG017288.
    InParanoidi Q569C3.
    KOi K11832.
    OMAi FAVVMHS.
    OrthoDBi EOG7JT6VZ.
    PhylomeDBi Q569C3.
    TreeFami TF331057.

    Miscellaneous databases

    NextBioi 666079.
    PROi Q569C3.

    Gene expression databases

    Genevestigatori Q569C3.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 2 hits.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Brown Norway.
      Tissue: TestisImported.

    Entry informationi

    Entry nameiUBP1_RAT
    AccessioniPrimary (citable) accession number: Q569C3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: May 10, 2005
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3