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Q569C3 (UBP1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 1

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 1
Ubiquitin thioesterase 1
Ubiquitin-specific-processing protease 1
Gene names
Name:Usp1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length784 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Interacts with FANCD2 and PCNA. Interacts with WDR48 By similarity.

Subcellular location

Nucleus By similarity UniProtKB O94782.

Post-translational modification

Autocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis By similarity.

Ubiquitinated; leading to its subsequent proteasomal degradation By similarity.

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 USP domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 784784Ubiquitin carboxyl-terminal hydrolase 1
PRO_0000306288

Regions

Domain81 – 784704USP

Sites

Active site901Nucleophile By similarity UniProtKB Q93009
Active site5931Proton acceptor By similarity UniProtKB Q93009
Site670 – 6712Cleavage; by autolysis By similarity UniProtKB O94782

Amino acid modifications

Modified residue421Phosphoserine By similarity UniProtKB O94782
Modified residue671Phosphoserine By similarity
Modified residue4751Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q569C3 [UniParc].

Last modified May 10, 2005. Version 1.
Checksum: 4CAFB369297DE7CC

FASTA78487,329
        10         20         30         40         50         60 
MPGVIPSESN GLSRGSPSKK NRLSLKFFQK KETKRALDFT DSQEDEEKAS EYRGSEIDQV 

        70         80         90        100        110        120 
VPAAQSSPVS CEKRENLLPF VGLNNLGNTC YLNSILQVLY FCPGFKAGVK HLFNIISRKK 

       130        140        150        160        170        180 
EALKDDSIQK DKGSCKEDPL ASYELICSLQ SLIISVEQLQ ASFLLNPEKY TDELATQPRR 

       190        200        210        220        230        240 
LLNTLRELNP MYEGYLQHDA QEVLQCILGN IQETCQLLKK EEIKNLTEFS SKVEEKSLQK 

       250        260        270        280        290        300 
EETGGISSTE TDSTRNLDDL KEQLPKGNWK RKSDGESGNM KKKVKLSRES QPLEENQRQT 

       310        320        330        340        350        360 
RSKRKATGDT LEASPKIIPK CVSENESAKP SQKKSKVKIN WLKPATKQPS ILSKFCSLGK 

       370        380        390        400        410        420 
ITTNQRSKGQ PKVNEGDLEE DLEKDGRDNT VNGSGPASPG SSVTPVDSSE AKSINKGAEQ 

       430        440        450        460        470        480 
IGFELVEKLF QGQLVLRTRC LECESLTERR EDFQDISVPV QEDELSKVEE SSEISPEPKT 

       490        500        510        520        530        540 
EMKTLRWAIS QFASVERIVG EDKYFCENCH HYTEAERSLL FDKMPEVITI HLKCFAASGL 

       550        560        570        580        590        600 
EFDCYGGGLS KINTPLLTPL KLSLEEWSTK PTNDSYGLFA VVMHSGITIS SGHYTASVKV 

       610        620        630        640        650        660 
TDLNSLELDK GNFVVDQMCE IGKPEPLNEE EARGTAENYD DEVSIRVGGN AQPSKVLNKK 

       670        680        690        700        710        720 
NVEGIGLLGG QKSKADYELC SKASNPEKVV GTPFTDSRNS ETNDTNGTQE SDRSKESSDQ 

       730        740        750        760        770        780 
TGINVSGLEN KISYVVQSLK EYEGKWLLFD DSEVKVTEEK DFLNSLSPST SPTSTPYLLF 


YKKL 

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References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Brown Norway.
Tissue: Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC092574 mRNA. Translation: AAH92574.1.
RefSeqNP_001015015.1. NM_001015015.1.
UniGeneRn.155728.

3D structure databases

ProteinModelPortalQ569C3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000010935.

Protein family/group databases

MEROPSC19.019.

PTM databases

PhosphoSiteQ569C3.

Proteomic databases

PRIDEQ569C3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000010933; ENSRNOP00000010935; ENSRNOG00000007890.
GeneID313387.
KEGGrno:313387.
UCSCRGD:1306461. rat.

Organism-specific databases

CTD7398.
RGD1306461. Usp1.

Phylogenomic databases

eggNOGCOG5077.
GeneTreeENSGT00650000093027.
HOGENOMHOG000154756.
HOVERGENHBG017288.
InParanoidQ569C3.
KOK11832.
OMAFAVVMHS.
OrthoDBEOG7JT6VZ.
PhylomeDBQ569C3.
TreeFamTF331057.

Gene expression databases

GenevestigatorQ569C3.

Family and domain databases

InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00443. UCH. 2 hits.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio666079.
PROQ569C3.

Entry information

Entry nameUBP1_RAT
AccessionPrimary (citable) accession number: Q569C3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: May 10, 2005
Last modified: April 16, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries