SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q569C3

- UBP1_RAT

UniProt

Q569C3 - UBP1_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Ubiquitin carboxyl-terminal hydrolase 1
Gene
Usp1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei90 – 901Nucleophile By similarityBy similarity
Active sitei593 – 5931Proton acceptor By similarityBy similarity
Sitei670 – 6712Cleavage; by autolysis By similarityBy similarity

GO - Molecular functioni

  1. ubiquitin thiolesterase activity Source: UniProtKB
  2. ubiquitin-specific protease activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. DNA repair Source: UniProtKB-KW
  2. monoubiquitinated protein deubiquitination Source: UniProtKB
  3. protein deubiquitination Source: UniProtKB
  4. regulation of DNA repair Source: UniProtKB
  5. response to UV Source: UniProtKB
  6. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.019.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 1 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 1
Ubiquitin thioesterase 1
Ubiquitin-specific-processing protease 1
Gene namesi
Name:Usp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 5

Organism-specific databases

RGDi1306461. Usp1.

Subcellular locationi

Nucleus By similarity By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 784784Ubiquitin carboxyl-terminal hydrolase 1
PRO_0000306288Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei42 – 421Phosphoserine By similarityBy similarity
Modified residuei67 – 671Phosphoserine By similarity
Modified residuei475 – 4751Phosphoserine By similarity

Post-translational modificationi

Autocatalytic cleavage of USP1 following UV irradiation inactivates it leading to an increase in ubiquitinated PCNA, recruitment of POLH and translesion synthesis By similarity.
Ubiquitinated; leading to its subsequent proteasomal degradation By similarity.

Keywords - PTMi

Autocatalytic cleavage, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ569C3.

PTM databases

PhosphoSiteiQ569C3.

Expressioni

Gene expression databases

GenevestigatoriQ569C3.

Interactioni

Subunit structurei

Interacts with FANCD2 and PCNA. Interacts with WDR48 By similarity.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010935.

Structurei

3D structure databases

ProteinModelPortaliQ569C3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini81 – 784704USP
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.
Contains 1 USP domain.

Phylogenomic databases

eggNOGiCOG5077.
GeneTreeiENSGT00650000093027.
HOGENOMiHOG000154756.
HOVERGENiHBG017288.
InParanoidiQ569C3.
KOiK11832.
OMAiFAVVMHS.
OrthoDBiEOG7JT6VZ.
PhylomeDBiQ569C3.
TreeFamiTF331057.

Family and domain databases

InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00443. UCH. 2 hits.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q569C3-1 [UniParc]FASTAAdd to Basket

« Hide

MPGVIPSESN GLSRGSPSKK NRLSLKFFQK KETKRALDFT DSQEDEEKAS    50
EYRGSEIDQV VPAAQSSPVS CEKRENLLPF VGLNNLGNTC YLNSILQVLY 100
FCPGFKAGVK HLFNIISRKK EALKDDSIQK DKGSCKEDPL ASYELICSLQ 150
SLIISVEQLQ ASFLLNPEKY TDELATQPRR LLNTLRELNP MYEGYLQHDA 200
QEVLQCILGN IQETCQLLKK EEIKNLTEFS SKVEEKSLQK EETGGISSTE 250
TDSTRNLDDL KEQLPKGNWK RKSDGESGNM KKKVKLSRES QPLEENQRQT 300
RSKRKATGDT LEASPKIIPK CVSENESAKP SQKKSKVKIN WLKPATKQPS 350
ILSKFCSLGK ITTNQRSKGQ PKVNEGDLEE DLEKDGRDNT VNGSGPASPG 400
SSVTPVDSSE AKSINKGAEQ IGFELVEKLF QGQLVLRTRC LECESLTERR 450
EDFQDISVPV QEDELSKVEE SSEISPEPKT EMKTLRWAIS QFASVERIVG 500
EDKYFCENCH HYTEAERSLL FDKMPEVITI HLKCFAASGL EFDCYGGGLS 550
KINTPLLTPL KLSLEEWSTK PTNDSYGLFA VVMHSGITIS SGHYTASVKV 600
TDLNSLELDK GNFVVDQMCE IGKPEPLNEE EARGTAENYD DEVSIRVGGN 650
AQPSKVLNKK NVEGIGLLGG QKSKADYELC SKASNPEKVV GTPFTDSRNS 700
ETNDTNGTQE SDRSKESSDQ TGINVSGLEN KISYVVQSLK EYEGKWLLFD 750
DSEVKVTEEK DFLNSLSPST SPTSTPYLLF YKKL 784
Length:784
Mass (Da):87,329
Last modified:May 10, 2005 - v1
Checksum:i4CAFB369297DE7CC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC092574 mRNA. Translation: AAH92574.1.
RefSeqiNP_001015015.1. NM_001015015.1.
UniGeneiRn.155728.

Genome annotation databases

EnsembliENSRNOT00000010933; ENSRNOP00000010935; ENSRNOG00000007890.
GeneIDi313387.
KEGGirno:313387.
UCSCiRGD:1306461. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC092574 mRNA. Translation: AAH92574.1 .
RefSeqi NP_001015015.1. NM_001015015.1.
UniGenei Rn.155728.

3D structure databases

ProteinModelPortali Q569C3.
ModBasei Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000010935.

Protein family/group databases

MEROPSi C19.019.

PTM databases

PhosphoSitei Q569C3.

Proteomic databases

PRIDEi Q569C3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000010933 ; ENSRNOP00000010935 ; ENSRNOG00000007890 .
GeneIDi 313387.
KEGGi rno:313387.
UCSCi RGD:1306461. rat.

Organism-specific databases

CTDi 7398.
RGDi 1306461. Usp1.

Phylogenomic databases

eggNOGi COG5077.
GeneTreei ENSGT00650000093027.
HOGENOMi HOG000154756.
HOVERGENi HBG017288.
InParanoidi Q569C3.
KOi K11832.
OMAi FAVVMHS.
OrthoDBi EOG7JT6VZ.
PhylomeDBi Q569C3.
TreeFami TF331057.

Miscellaneous databases

NextBioi 666079.
PROi Q569C3.

Gene expression databases

Genevestigatori Q569C3.

Family and domain databases

InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00443. UCH. 2 hits.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Brown Norway.
    Tissue: Testis.

Entry informationi

Entry nameiUBP1_RAT
AccessioniPrimary (citable) accession number: Q569C3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: May 10, 2005
Last modified: April 16, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi