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Protein

IST1 homolog

Gene

Ist1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

ESCRT-III-like protein involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing. Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Names & Taxonomyi

Protein namesi
Recommended name:
IST1 homolog
Gene namesi
Name:Ist1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi1307799. Ist1.

Subcellular locationi

  • Cytoplasmic vesicle By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
  • Midbody By similarity
  • Nucleus envelope By similarity

  • Note: Localizes to centrosome and midbody of dividing cells. Colocalized with SPG20 to the ends of Flemming bodies during cytokinesis. Localizes to the nuclear envelope during late anaphase.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 366366IST1 homologPRO_0000327595Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei43 – 431PhosphotyrosineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ568Z6.
PRIDEiQ568Z6.

PTM databases

iPTMnetiQ568Z6.
PhosphoSiteiQ568Z6.

Expressioni

Gene expression databases

GenevisibleiQ568Z6. RN.

Interactioni

Subunit structurei

Interacts with CHMP1A, CHMP1B, VPS4A and VTA1. Interacts with SPAST, STAMBP, and USP8. May interact with VPS37B. May associate with the ESCRT-I complex. Interacts with MITD1, in competition with VSP4. Interacts with SPG20 (via MIT domain); leading to the recruitement of SPG20 to midbodies. Interacts with SPAST.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020365.

Family & Domainsi

Sequence similaritiesi

Belongs to the IST1 family.Curated

Phylogenomic databases

eggNOGiKOG2027. Eukaryota.
ENOG410YG52. LUCA.
GeneTreeiENSGT00390000007453.
HOGENOMiHOG000205346.
InParanoidiQ568Z6.
KOiK19476.
OMAiDTYDNFV.
OrthoDBiEOG7K3TNC.
PhylomeDBiQ568Z6.
TreeFamiTF314258.

Family and domain databases

InterProiIPR005061. Ist1.
[Graphical view]
PANTHERiPTHR12161. PTHR12161. 1 hit.
PfamiPF03398. Ist1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q568Z6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGSGFKAER LRVNLRLVIN RLKLLEKKKT ELAQKARKEI ADYLAAGKDE
60 70 80 90 100
RARIRVEHII REDYLVEAME ILELYCDLLL ARFGLIQSMK ELDSGLAESV
110 120 130 140 150
STLIWAAPRL QSEVAELKIV ADQLCAKYSK EYGKLCRTNQ IGTVNDRLMH
160 170 180 190 200
KLSVEAPPKI LVERYLIEIA KNYNVPYEPD SVVMAEAPVG VETDLIDVGF
210 220 230 240 250
TDDVKKGGPG RGGGGGFTAP VGAPDGTMPM PMPMPMPMPS PSPNAPFAYP
260 270 280 290 300
LPKGPSDFSG LPVGTYQAFP NIHPPQIPAT PPSYESVDDI NADKNVSSAQ
310 320 330 340 350
IVGPKPEAPA KPPSRPVDNY NTFVLPELPS VPDTLPTASA GASTSASEDI
360
DFDDLSRRFE ELKKKT
Length:366
Mass (Da):39,942
Last modified:May 10, 2005 - v1
Checksum:iDDFC8E43B0C6DFEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC092631 mRNA. Translation: AAH92631.1.
RefSeqiNP_001017454.1. NM_001017454.2.
UniGeneiRn.2250.

Genome annotation databases

EnsembliENSRNOT00000020365; ENSRNOP00000020365; ENSRNOG00000015144.
GeneIDi307833.
KEGGirno:307833.
UCSCiRGD:1307799. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC092631 mRNA. Translation: AAH92631.1.
RefSeqiNP_001017454.1. NM_001017454.2.
UniGeneiRn.2250.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020365.

PTM databases

iPTMnetiQ568Z6.
PhosphoSiteiQ568Z6.

Proteomic databases

PaxDbiQ568Z6.
PRIDEiQ568Z6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000020365; ENSRNOP00000020365; ENSRNOG00000015144.
GeneIDi307833.
KEGGirno:307833.
UCSCiRGD:1307799. rat.

Organism-specific databases

CTDi9798.
RGDi1307799. Ist1.

Phylogenomic databases

eggNOGiKOG2027. Eukaryota.
ENOG410YG52. LUCA.
GeneTreeiENSGT00390000007453.
HOGENOMiHOG000205346.
InParanoidiQ568Z6.
KOiK19476.
OMAiDTYDNFV.
OrthoDBiEOG7K3TNC.
PhylomeDBiQ568Z6.
TreeFamiTF314258.

Miscellaneous databases

NextBioi657944.
PROiQ568Z6.

Gene expression databases

GenevisibleiQ568Z6. RN.

Family and domain databases

InterProiIPR005061. Ist1.
[Graphical view]
PANTHERiPTHR12161. PTHR12161. 1 hit.
PfamiPF03398. Ist1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiIST1_RAT
AccessioniPrimary (citable) accession number: Q568Z6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 10, 2005
Last modified: May 11, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.