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Protein

E3 ubiquitin-protein ligase RNF185

Gene

Rnf185

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating 'Lys-63'-linked polyubiquitination of BNIP1. Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway. Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2 (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri39 – 80RING-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Autophagy, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-382556. ABC-family proteins mediated transport.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF185 (EC:6.3.2.-)
Alternative name(s):
RING finger protein 185
Gene namesi
Name:Rnf185
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi1564777. Rnf185.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 130CytoplasmicAdd BLAST130
Transmembranei131 – 151HelicalSequence analysisAdd BLAST21
Topological domaini152 – 171Mitochondrial intermembraneAdd BLAST20
Transmembranei172 – 192HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002475231 – 192E3 ubiquitin-protein ligase RNF185Add BLAST192

Proteomic databases

PaxDbiQ568Y3.

PTM databases

PhosphoSitePlusiQ568Y3.

Expressioni

Gene expression databases

BgeeiENSRNOG00000019325.
GenevisibleiQ568Y3. RN.

Interactioni

Subunit structurei

Interacts with ATG5 and BNIP1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026134.

Structurei

3D structure databases

ProteinModelPortaliQ568Y3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity.By similarity

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri39 – 80RING-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG0823. Eukaryota.
ENOG4111IHV. LUCA.
GeneTreeiENSGT00390000014107.
HOGENOMiHOG000238304.
HOVERGENiHBG054495.
InParanoidiQ568Y3.
KOiK10666.
OMAiPNRQLCP.
OrthoDBiEOG091G0W2I.
PhylomeDBiQ568Y3.
TreeFamiTF317334.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q568Y3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASKGPSASA STENSSAGGP SGSSNGTGES GGQDSTFECN ICLDTAKDAV
60 70 80 90 100
ISLCGHLFCW PCLHQWLETR PNRQVCPVCK AGISRDKVIP LYGRGSTGQQ
110 120 130 140 150
DPREKTPPRP QGQRPEPENR GGFQGFGFGD GGFQMSFGIG AFPFGIFATA
160 170 180 190
FNINDGRPPP AVPGTPQYVD EQFLSRLFLF VALVIMFWLL IA
Length:192
Mass (Da):20,493
Last modified:May 10, 2005 - v1
Checksum:iBB3F58FF557089DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC092655 mRNA. Translation: AAH92655.1.
RefSeqiNP_001019442.1. NM_001024271.1.
XP_006251395.1. XM_006251333.2.
XP_017454807.1. XM_017599318.1.
UniGeneiRn.7234.

Genome annotation databases

EnsembliENSRNOT00000026134; ENSRNOP00000026134; ENSRNOG00000019325.
GeneIDi360967.
KEGGirno:360967.
UCSCiRGD:1564777. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC092655 mRNA. Translation: AAH92655.1.
RefSeqiNP_001019442.1. NM_001024271.1.
XP_006251395.1. XM_006251333.2.
XP_017454807.1. XM_017599318.1.
UniGeneiRn.7234.

3D structure databases

ProteinModelPortaliQ568Y3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026134.

PTM databases

PhosphoSitePlusiQ568Y3.

Proteomic databases

PaxDbiQ568Y3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000026134; ENSRNOP00000026134; ENSRNOG00000019325.
GeneIDi360967.
KEGGirno:360967.
UCSCiRGD:1564777. rat.

Organism-specific databases

CTDi91445.
RGDi1564777. Rnf185.

Phylogenomic databases

eggNOGiKOG0823. Eukaryota.
ENOG4111IHV. LUCA.
GeneTreeiENSGT00390000014107.
HOGENOMiHOG000238304.
HOVERGENiHBG054495.
InParanoidiQ568Y3.
KOiK10666.
OMAiPNRQLCP.
OrthoDBiEOG091G0W2I.
PhylomeDBiQ568Y3.
TreeFamiTF317334.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-RNO-382556. ABC-family proteins mediated transport.

Miscellaneous databases

PROiQ568Y3.

Gene expression databases

BgeeiENSRNOG00000019325.
GenevisibleiQ568Y3. RN.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRN185_RAT
AccessioniPrimary (citable) accession number: Q568Y3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: May 10, 2005
Last modified: November 30, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.