Q568I2 (ACER1_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alkaline ceramidase 1
Short name=AlkCDase 1
Short name=Alkaline CDase 1
EC=3.5.1.23

Alternative name(s):
Acylsphingosine deacylase 3
N-acylsphingosine amidohydrolase 3

Gene names

Name:	acer1
Synonyms:	asah3
ORF Names:	zgc:110285

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 8.0 By similarity.
Catalytic activity	N-acylsphingosine + H2O = a carboxylate + sphingosine.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the alkaline ceramidase family.

Ontologies

Keywords
Biological process	Lipid metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ceramide metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ceramidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 266	266	Alkaline ceramidase 1		PRO_0000247747
Regions
	Topological domain	1 – 27	27	Lumenal Potential
	Transmembrane	28 – 48	21	Helical; Potential
	Topological domain	49 – 56	8	Cytoplasmic Potential
	Transmembrane	57 – 77	21	Helical; Potential
	Topological domain	78 – 83	6	Lumenal Potential
	Transmembrane	84 – 104	21	Helical; Potential
	Topological domain	105 – 116	12	Cytoplasmic Potential
	Transmembrane	117 – 136	20	Helical; Potential
	Topological domain	137 – 139	3	Lumenal Potential
	Transmembrane	140 – 160	21	Helical; Potential
	Topological domain	161 – 167	7	Cytoplasmic Potential
	Transmembrane	168 – 188	21	Helical; Potential
	Topological domain	189 – 205	17	Lumenal Potential
	Transmembrane	206 – 226	21	Helical; Potential
	Topological domain	227 – 266	40	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	Q568I2 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 0619331570BE647C	FASTA	266	30,908
	10 20 30 40 50 60 MAGVFSYESS EVDWCEDNYK HSENVVEYFN TMSSFIFFVI SPIMLYLLHP YAKERNLAVH 70 80 90 100 110 120 LVWIMMVFVG IFSMYFHMTL SFMGQMLDEL SILWVLAIGY SLWFPRKHFP SFVKDRTSFA 130 140 150 160 170 180 RLVLTITIIS TLSSFVKPTA NAYALNCFAI HILYSLFVEL KSCTDERVLR LAWASIGLWV 190 200 210 220 230 240 LAISCWISDR FGCSFWQKLD FCYLHGIWHI LIVMATAYAS TLIAYLDASQ EIPYSLPDLQ 250 260 YWPRNNWAIG LPYIVLKGTN TTRKTC

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Olfactory epithelium.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC092849 mRNA. Translation: AAH92849.1.
IPI	IPI00499405.
RefSeq	NP_001017603.1. NM_001017603.1.
UniGene	Dr.80506.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q568I2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	550266.
KEGG	dre:550266.
Organism-specific databases
CTD	125981.
ZFIN	ZDB-GENE-050417-70. acer1.
Phylogenomic databases
eggNOG	fiNOG10726.
GeneTree	ENSGT00390000004916.
InParanoid	Q568I2.
OrthoDB	EOG4XD3RW.
PhylomeDB	Q568I2.
Gene expression databases
ArrayExpress	Q568I2.
Bgee	Q568I2.
Family and domain databases
InterPro	IPR008901. Ceramidase. [Graphical view]
KO	K01441.
Pfam	PF05875. Ceramidase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACER1_DANRE

Accession

Primary (citable) accession number: Q568I2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	May 10, 2005
Last modified:	November 16, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program