Reviewed, UniProtKB/Swiss-Prot Q568I2 (ACER1_DANRE)

Names and origin

Protein names

Recommended name:
    Alkaline ceramidase 1
      Short name=Alkaline CDase 1
      Short name=AlkCDase 1
    EC=3.5.1.23
Alternative name(s):
    N-acylsphingosine amidohydrolase 3
    Acylsphingosine deacylase 3

Gene names

Name:	acer1
Synonyms:	asah3
ORF Names:	zgc:110285

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 8.0 By similarity.
Catalytic activity	N-acylsphingosine + H2O = a carboxylate + sphingosine.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the alkaline ceramidase family.

Ontologies

Keywords
Biological process	Lipid metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	ceramide metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: InterPro endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ceramidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 266	266	Alkaline ceramidase 1		PRO_0000247747
Regions
	Transmembrane	28 – 48	21	Potential
	Transmembrane	57 – 77	21	Potential
	Transmembrane	84 – 104	21	Potential
	Transmembrane	117 – 136	20	Potential
	Transmembrane	140 – 160	21	Potential
	Transmembrane	168 – 188	21	Potential
	Transmembrane	206 – 226	21	Potential
Amino acid modifications
	Glycosylation	260	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q568I2-1 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 0619331570BE647C	FASTA	266	30,908
	10 20 30 40 50 60 MAGVFSYESS EVDWCEDNYK HSENVVEYFN TMSSFIFFVI SPIMLYLLHP YAKERNLAVH 70 80 90 100 110 120 LVWIMMVFVG IFSMYFHMTL SFMGQMLDEL SILWVLAIGY SLWFPRKHFP SFVKDRTSFA 130 140 150 160 170 180 RLVLTITIIS TLSSFVKPTA NAYALNCFAI HILYSLFVEL KSCTDERVLR LAWASIGLWV 190 200 210 220 230 240 LAISCWISDR FGCSFWQKLD FCYLHGIWHI LIVMATAYAS TLIAYLDASQ EIPYSLPDLQ 250 260 YWPRNNWAIG LPYIVLKGTN TTRKTC

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Olfactory epithelium.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC092849 mRNA. Translation: AAH92849.1.
IPI	IPI00499405.
RefSeq	NP_001017603.1.
UniGene	Dr.80506
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q568I2.
Genome annotation databases
Ensembl	ENSDART00000023797; ENSDARP00000022832; ENSDARG00000010347; Danio rerio. [Genome view]
GeneID	550266.
KEGG	dre:550266.
Organism-specific databases
CTD	550266.
ZFIN	ZDB-GENE-050417-70. acer1.
Phylogenomic databases
eggNOG	fiNOG10726.
HOVERGEN	Q568I2.
InParanoid	Q568I2.
PhylomeDB	Q568I2.
Enzyme and pathway databases
BRENDA	3.5.1.23. 96826.
Gene expression databases
ArrayExpress	Q568I2.
Bgee	Q568I2.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

ACER1_DANRE

Accession

Primary (citable) accession number: Q568I2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	May 10, 2005
Last modified:	February 9, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project