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Protein

Protein UshA

Gene

ushA

Organism
Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Degradation of external UDP-glucose to uridine monophosphate and glucose-1-phosphate, which can then be used by the cell.By similarity

Catalytic activityi

UDP-sugar + H2O = UMP + alpha-D-aldose 1-phosphate.
A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

Cofactori

Co2+By similarityNote: Divalent metal cations. Most likely Co2+.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi41Divalent metal cation 1By similarity1
Metal bindingi43Divalent metal cation 1By similarity1
Metal bindingi84Divalent metal cation 1By similarity1
Metal bindingi84Divalent metal cation 2By similarity1
Metal bindingi116Divalent metal cation 2By similarity1
Sitei117Transition state stabilizerBy similarity1
Sitei120Transition state stabilizerBy similarity1
Metal bindingi217Divalent metal cation 2By similarity1
Metal bindingi252Divalent metal cation 2By similarity1
Metal bindingi254Divalent metal cation 1By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Cobalt, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein UshA
Including the following 2 domains:
UDP-sugar hydrolase (EC:3.6.1.45)
Alternative name(s):
UDP-sugar diphosphatase
UDP-sugar pyrophosphatase
5'-nucleotidase (EC:3.1.3.5)
Short name:
5'-NT
Gene namesi
Name:ushA
Ordered Locus Names:YE3066
OrganismiYersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
Taxonomic identifieri393305 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000642 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000000003426 – 550Protein UshAAdd BLAST525

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi258 ↔ 275By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ56878.

Interactioni

Protein-protein interaction databases

STRINGi393305.YE3066.

Structurei

3D structure databases

ProteinModelPortaliQ56878.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni375 – 379Substrate bindingBy similarity5
Regioni498 – 504Substrate bindingBy similarity7

Sequence similaritiesi

Belongs to the 5'-nucleotidase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105CGH. Bacteria.
COG0737. LUCA.
HOGENOMiHOG000247216.
KOiK11751.
OMAiAADYEPK.
OrthoDBiPOG091H03VR.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProiIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PANTHERiPTHR11575. PTHR11575. 1 hit.
PfamiPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR01607. APYRASEFAMLY.
SUPFAMiSSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q56878-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFSLSTTAA ALAVSLAFAP GWAVAWEKDK TYDITILHTN DHHGHFWQND
60 70 80 90 100
HGEYGLAAQK TLVDDIRKQV AAAGGSLLLL SGGDINTGVP ESDLQDAEPD
110 120 130 140 150
FRGMNLVGYD AMAIGNHEFD NPLSVLRQQE KWATFPLLSA NIYQKSTQQR
160 170 180 190 200
LFKPYALFDK QGVKIAVIGL TTDDTAKIGN PEYFTDIEFR VPATEAKQVV
210 220 230 240 250
EQLRKTEKPD IIIAATHMGH YDDGKHGSNA PGDVEMARSL PAGYLDMIVG
260 270 280 290 300
GHSQDPVCMA SENHKQADYV PGTPCAPDRQ NGTWIVQAHE WGKYVGRADF
310 320 330 340 350
KFRNGELKLV SYQLIPINLK KKVEKADGTS ERIFYTQEIA QDPSMLKLLT
360 370 380 390 400
PFEQQGKAQL DVKVGSVNGK LEGDRSKVRF EQTNLARLLL AAQMERAGAD
410 420 430 440 450
FAVMSGGGVR DSIDAGDITY KDVLKVQPFG NTLVYADMKG SEVEKYLAVV
460 470 480 490 500
ANKKVDSGAY AQFANVSLVA DGKGVSNVKI QGKPLDPNKT YRLATLNFNA
510 520 530 540 550
LGGDGYPKID TLPSYVNTGF IDAEVLKQYI EKHSPLDASQ YQPKGEIVYK
Length:550
Mass (Da):60,383
Last modified:March 20, 2007 - v3
Checksum:iE52BA3FD5A71ED61
GO

Sequence cautioni

The sequence AAC60782 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24V → A in AAC60782 (Ref. 2) Curated1
Sequence conflicti47W → C in AAC60782 (Ref. 2) Curated1
Sequence conflicti72 – 83AAGGS…LLSGG → RSRGKLVVALRW in AAC60782 (Ref. 2) CuratedAdd BLAST12
Sequence conflicti126Missing in AAC60782 (Ref. 2) Curated1
Sequence conflicti129 – 130QE → HQ in AAC60782 (Ref. 2) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM286415 Genomic DNA. Translation: CAL13101.1.
U46859 Genomic DNA. Translation: AAC60782.1. Different initiation.
RefSeqiWP_011816863.1. NC_008800.1.
YP_001007248.1. NC_008800.1.

Genome annotation databases

EnsemblBacteriaiCAL13101; CAL13101; YE3066.
GeneIDi4714553.
KEGGiyen:YE3066.
PATRICi18565839. VBIYerEnt11519_3261.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM286415 Genomic DNA. Translation: CAL13101.1.
U46859 Genomic DNA. Translation: AAC60782.1. Different initiation.
RefSeqiWP_011816863.1. NC_008800.1.
YP_001007248.1. NC_008800.1.

3D structure databases

ProteinModelPortaliQ56878.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi393305.YE3066.

Proteomic databases

PRIDEiQ56878.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL13101; CAL13101; YE3066.
GeneIDi4714553.
KEGGiyen:YE3066.
PATRICi18565839. VBIYerEnt11519_3261.

Phylogenomic databases

eggNOGiENOG4105CGH. Bacteria.
COG0737. LUCA.
HOGENOMiHOG000247216.
KOiK11751.
OMAiAADYEPK.
OrthoDBiPOG091H03VR.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProiIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PANTHERiPTHR11575. PTHR11575. 1 hit.
PfamiPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR01607. APYRASEFAMLY.
SUPFAMiSSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUSHA_YERE8
AccessioniPrimary (citable) accession number: Q56878
Secondary accession number(s): A1JN00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 20, 2007
Last modified: November 30, 2016
This is version 114 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.