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Q56878

- USHA_YERE8

UniProt

Q56878 - USHA_YERE8

Protein

Protein UshA

Gene

ushA

Organism
Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 3 (20 Mar 2007)
      Previous versions | rss
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    Functioni

    Degradation of external UDP-glucose to uridine monophosphate and glucose-1-phosphate, which can then be used by the cell.By similarity

    Catalytic activityi

    UDP-sugar + H2O = UMP + alpha-D-aldose 1-phosphate.
    A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

    Cofactori

    Divalent cations. Most likely cobalt By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi41 – 411Divalent metal cation 1By similarity
    Metal bindingi43 – 431Divalent metal cation 1By similarity
    Metal bindingi84 – 841Divalent metal cation 1By similarity
    Metal bindingi84 – 841Divalent metal cation 2By similarity
    Metal bindingi116 – 1161Divalent metal cation 2By similarity
    Sitei117 – 1171Transition state stabilizerBy similarity
    Sitei120 – 1201Transition state stabilizerBy similarity
    Metal bindingi217 – 2171Divalent metal cation 2By similarity
    Metal bindingi252 – 2521Divalent metal cation 2By similarity
    Metal bindingi254 – 2541Divalent metal cation 1By similarity

    GO - Molecular functioni

    1. 5'-nucleotidase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW
    3. nucleotide binding Source: UniProtKB-KW
    4. UDP-sugar diphosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. nucleotide catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Cobalt, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein UshA
    Including the following 2 domains:
    UDP-sugar hydrolase (EC:3.6.1.45)
    Alternative name(s):
    UDP-sugar diphosphatase
    UDP-sugar pyrophosphatase
    5'-nucleotidase (EC:3.1.3.5)
    Short name:
    5'-NT
    Gene namesi
    Name:ushA
    Ordered Locus Names:YE3066
    OrganismiYersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
    Taxonomic identifieri393305 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
    ProteomesiUP000000642: Chromosome

    Subcellular locationi

    Periplasm Curated

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Chaini26 – 550525Protein UshAPRO_0000000034Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi258 ↔ 275By similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PRIDEiQ56878.

    Interactioni

    Protein-protein interaction databases

    STRINGi393305.YE3066.

    Structurei

    3D structure databases

    ProteinModelPortaliQ56878.
    SMRiQ56878. Positions 26-547.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni375 – 3795Substrate bindingBy similarity
    Regioni498 – 5047Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the 5'-nucleotidase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0737.
    HOGENOMiHOG000247216.
    KOiK11751.
    OMAiPFQDKGK.
    OrthoDBiEOG696BW0.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    3.90.780.10. 1 hit.
    InterProiIPR008334. 5'-Nucleotdase_C.
    IPR006146. 5'-Nucleotdase_CS.
    IPR006179. 5_nucleotidase/apyrase.
    IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    [Graphical view]
    PANTHERiPTHR11575. PTHR11575. 1 hit.
    PfamiPF02872. 5_nucleotid_C. 1 hit.
    PF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR01607. APYRASEFAMLY.
    SUPFAMiSSF55816. SSF55816. 1 hit.
    SSF56300. SSF56300. 1 hit.
    PROSITEiPS00785. 5_NUCLEOTIDASE_1. 1 hit.
    PS00786. 5_NUCLEOTIDASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q56878-1 [UniParc]FASTAAdd to Basket

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    MRFSLSTTAA ALAVSLAFAP GWAVAWEKDK TYDITILHTN DHHGHFWQND    50
    HGEYGLAAQK TLVDDIRKQV AAAGGSLLLL SGGDINTGVP ESDLQDAEPD 100
    FRGMNLVGYD AMAIGNHEFD NPLSVLRQQE KWATFPLLSA NIYQKSTQQR 150
    LFKPYALFDK QGVKIAVIGL TTDDTAKIGN PEYFTDIEFR VPATEAKQVV 200
    EQLRKTEKPD IIIAATHMGH YDDGKHGSNA PGDVEMARSL PAGYLDMIVG 250
    GHSQDPVCMA SENHKQADYV PGTPCAPDRQ NGTWIVQAHE WGKYVGRADF 300
    KFRNGELKLV SYQLIPINLK KKVEKADGTS ERIFYTQEIA QDPSMLKLLT 350
    PFEQQGKAQL DVKVGSVNGK LEGDRSKVRF EQTNLARLLL AAQMERAGAD 400
    FAVMSGGGVR DSIDAGDITY KDVLKVQPFG NTLVYADMKG SEVEKYLAVV 450
    ANKKVDSGAY AQFANVSLVA DGKGVSNVKI QGKPLDPNKT YRLATLNFNA 500
    LGGDGYPKID TLPSYVNTGF IDAEVLKQYI EKHSPLDASQ YQPKGEIVYK 550
    Length:550
    Mass (Da):60,383
    Last modified:March 20, 2007 - v3
    Checksum:iE52BA3FD5A71ED61
    GO

    Sequence cautioni

    The sequence AAC60782.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti24 – 241V → A in AAC60782. 1 PublicationCurated
    Sequence conflicti47 – 471W → C in AAC60782. 1 PublicationCurated
    Sequence conflicti72 – 8312AAGGS…LLSGG → RSRGKLVVALRW in AAC60782. 1 PublicationCuratedAdd
    BLAST
    Sequence conflicti126 – 1261Missing in AAC60782. 1 PublicationCurated
    Sequence conflicti129 – 1302QE → HQ in AAC60782. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM286415 Genomic DNA. Translation: CAL13101.1.
    U46859 Genomic DNA. Translation: AAC60782.1. Different initiation.
    RefSeqiYP_001007248.1. NC_008800.1.

    Genome annotation databases

    EnsemblBacteriaiCAL13101; CAL13101; YE3066.
    GeneIDi4714553.
    KEGGiyen:YE3066.
    PATRICi18565839. VBIYerEnt11519_3261.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM286415 Genomic DNA. Translation: CAL13101.1 .
    U46859 Genomic DNA. Translation: AAC60782.1 . Different initiation.
    RefSeqi YP_001007248.1. NC_008800.1.

    3D structure databases

    ProteinModelPortali Q56878.
    SMRi Q56878. Positions 26-547.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 393305.YE3066.

    Proteomic databases

    PRIDEi Q56878.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAL13101 ; CAL13101 ; YE3066 .
    GeneIDi 4714553.
    KEGGi yen:YE3066.
    PATRICi 18565839. VBIYerEnt11519_3261.

    Phylogenomic databases

    eggNOGi COG0737.
    HOGENOMi HOG000247216.
    KOi K11751.
    OMAi PFQDKGK.
    OrthoDBi EOG696BW0.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    3.90.780.10. 1 hit.
    InterProi IPR008334. 5'-Nucleotdase_C.
    IPR006146. 5'-Nucleotdase_CS.
    IPR006179. 5_nucleotidase/apyrase.
    IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    [Graphical view ]
    PANTHERi PTHR11575. PTHR11575. 1 hit.
    Pfami PF02872. 5_nucleotid_C. 1 hit.
    PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR01607. APYRASEFAMLY.
    SUPFAMi SSF55816. SSF55816. 1 hit.
    SSF56300. SSF56300. 1 hit.
    PROSITEi PS00785. 5_NUCLEOTIDASE_1. 1 hit.
    PS00786. 5_NUCLEOTIDASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NCTC 13174 / 8081.
    2. Zhang L., Toivanen P., Skurnik M.
      Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-374.

    Entry informationi

    Entry nameiUSHA_YERE8
    AccessioniPrimary (citable) accession number: Q56878
    Secondary accession number(s): A1JN00
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: March 20, 2007
    Last modified: October 1, 2014
    This is version 100 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3