Reviewed, UniProtKB/Swiss-Prot Q56878 (USHA_YERE8)

Names and origin

Protein names

Recommended name:
    Protein ushA
Including the following 2 domains:
    1- Recommended name:
            UDP-sugar hydrolase
              EC=3.6.1.45
        Alternative name(s):
            UDP-sugar pyrophosphatase
            UDP-sugar diphosphatase
    2- Recommended name:
            5'-nucleotidase
                Short name=5'-NT
              EC=3.1.3.5

Gene names

Name:	ushA
Ordered Locus Names:	YE3066

Organism

Yersinia enterocolitica serotype O:8 / biotype 1B (strain 8081) [Complete proteome] [HAMAP]

Taxonomic identifier

393305 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	550 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Degradation of external UDP-glucose to uridine monophosphate and glucose-1-phosphate, which can then be used by the cell By similarity.
Catalytic activity	UDP-sugar + H2O = UMP + alpha-D-aldose 1-phosphate. A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.
Cofactor	Divalent cations. Most likely cobalt By similarity.
Subcellular location	Periplasm Potential.
Sequence similarities	Belongs to the 5'-nucleotidase family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Cobalt Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	5'-nucleotidase activity Inferred from electronic annotation. Source: EC UDP-sugar diphosphatase activity Inferred from electronic annotation. Source: EC cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 550	525	Protein ushA		PRO_0000000034
Regions
	Region	375 – 379	5	Substrate binding By similarity
	Region	498 – 504	7	Substrate binding By similarity
Sites
	Metal binding	41	1	Divalent metal cation 1 By similarity
	Metal binding	43	1	Divalent metal cation 1 By similarity
	Metal binding	84	1	Divalent metal cation 1 By similarity
	Metal binding	84	1	Divalent metal cation 2 By similarity
	Metal binding	116	1	Divalent metal cation 2 By similarity
	Metal binding	217	1	Divalent metal cation 2 By similarity
	Metal binding	252	1	Divalent metal cation 2 By similarity
	Metal binding	254	1	Divalent metal cation 1 By similarity
	Site	117	1	Transition state stabilizer By similarity
	Site	120	1	Transition state stabilizer By similarity
Amino acid modifications
	Disulfide bond	258 ↔ 275		By similarity
Experimental info
	Sequence conflict	24	1	V → A in AAC60782. Ref.2
	Sequence conflict	47	1	W → C in AAC60782. Ref.2
	Sequence conflict	72 – 83	12	AAGGS…LLSGG → RSRGKLVVALRW in AAC60782. Ref.2
	Sequence conflict	126	1	Missing in AAC60782. Ref.2
	Sequence conflict	129 – 130	2	QE → HQ in AAC60782. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q56878-1 [UniParc]. Last modified March 20, 2007. Version 3. Checksum: E52BA3FD5A71ED61	FASTA	550	60,383
	10 20 30 40 50 60 MRFSLSTTAA ALAVSLAFAP GWAVAWEKDK TYDITILHTN DHHGHFWQND HGEYGLAAQK 70 80 90 100 110 120 TLVDDIRKQV AAAGGSLLLL SGGDINTGVP ESDLQDAEPD FRGMNLVGYD AMAIGNHEFD 130 140 150 160 170 180 NPLSVLRQQE KWATFPLLSA NIYQKSTQQR LFKPYALFDK QGVKIAVIGL TTDDTAKIGN 190 200 210 220 230 240 PEYFTDIEFR VPATEAKQVV EQLRKTEKPD IIIAATHMGH YDDGKHGSNA PGDVEMARSL 250 260 270 280 290 300 PAGYLDMIVG GHSQDPVCMA SENHKQADYV PGTPCAPDRQ NGTWIVQAHE WGKYVGRADF 310 320 330 340 350 360 KFRNGELKLV SYQLIPINLK KKVEKADGTS ERIFYTQEIA QDPSMLKLLT PFEQQGKAQL 370 380 390 400 410 420 DVKVGSVNGK LEGDRSKVRF EQTNLARLLL AAQMERAGAD FAVMSGGGVR DSIDAGDITY 430 440 450 460 470 480 KDVLKVQPFG NTLVYADMKG SEVEKYLAVV ANKKVDSGAY AQFANVSLVA DGKGVSNVKI 490 500 510 520 530 540 QGKPLDPNKT YRLATLNFNA LGGDGYPKID TLPSYVNTGF IDAEVLKQYI EKHSPLDASQ 550 YQPKGEIVYK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence and comparative genome analysis of the high pathogenicity Yersinia enterocolitica strain 8081." Thomson N.R., Howard S., Wren B.W., Holden M.T.G., Crossman L., Challis G.L., Churcher C., Mungall K., Brooks K., Chillingworth T., Feltwell T., Abdellah Z., Hauser H., Jagels K., Maddison M., Moule S., Sanders M., Whitehead S. , Quail M.A., Dougan G., Parkhill J., Prentice M.B. PLoS Genet. 2:2039-2051(2006) [PubMed: 17173484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	Zhang L., Toivanen P., Skurnik M. Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-374.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM286415 Genomic DNA. Translation: CAL13101.1. U46859 Genomic DNA. Translation: AAC60782.1. Different initiation.
RefSeq	YP_001007248.1.
3D structure databases
SMR	Q56878. Positions 26-547.
ModBase	Search...
Protein-protein interaction databases
STRING	Q56878.
Genome annotation databases
GeneID	4714553.
GenomeReviews	Gene locus YE3066 in contig AM286415_GR.
KEGG	yen:YE3066.
NMPDR	fig|630.2.peg.2998.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0737.
HOGENOM	HBG534106.
OMA	CAPDRQN.
PhylomeDB	Q56878.
Family and domain databases
InterPro	IPR008334. 5'-Nucleotdase_C. IPR006146. 5'-Nucleotdase_CS. IPR006179. 5_nucleotidase/apyrase. IPR004843. M-pesterase. [Graphical view]
Gene3D	G3DSA:3.90.780.10. 5'-Nucleotdase_C. 1 hit.
PANTHER	PTHR11575. 5_nucleotidase. 1 hit.
Pfam	PF02872. 5_nucleotid_C. 1 hit. PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR01607. APYRASEFAMLY.
PROSITE	PS00785. 5_NUCLEOTIDASE_1. 1 hit. PS00786. 5_NUCLEOTIDASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

USHA_YERE8

Accession

Primary (citable) accession number: Q56878
Secondary accession number(s): A1JN00

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	March 20, 2007
Last modified:	January 19, 2010
This is version 71 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)