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Reviewed, UniProtKB/Swiss-Prot Q56841 (HCDS_XANP2)

Last modified June 16, 2009. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    2-(S)-hydroxypropyl-CoM dehydrogenase
      Short name=S-HPCDH
    EC=1.1.1.269
Alternative name(s):
    Aliphatic epoxide carboxylation component IV
Gene names
Name: xecE
Ordered Locus Names: Xaut_4869
Encoded onPlasmid pXAUT01
OrganismXanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) [Complete proteome] [HAMAP]
Taxonomic identifier78245 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeXanthobacter

Protein attributes

Sequence length249 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Catalyzes the oxidation of 2-(S)-hydroxyalkyl thioesters of CoM to 2-oxoalkyl thioesters of CoM. The enzyme highly specific for the S enantiomers.

Catalytic activity

2-(S)-hydroxypropyl-CoM + NAD+ = 2-oxopropyl-CoM + NADH.

Pathway

Alkene metabolism; propylene degradation.

Subunit structure

Homodimer. Component IV of the aliphatic epoxide carboxylation complex together with components I, II and III.

Induction

By aliphatic and chlorinated alkenes.

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Direct protein sequencing
Plasmid
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular function2-(S)-hydroxypropyl-CoM dehydrogenase activity

Inferred from electronic annotation. Source: EC

binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2492492-(S)-hydroxypropyl-CoM dehydrogenase
PRO_0000054706

Sites

Active site1501Proton acceptor By similarity
Binding site1371Substrate By similarity

Experimental info

Sequence conflict41A → N AA sequence Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q56841-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: B5E0B82C1D8D9782

FASTA24924,941
        10         20         30         40         50         60 
MLDAEVIAIT GGAAGIGLAV AHAAIRAGAR VALIDRDGAC AQRAAAEFGA AAWGVGADVT 

        70         80         90        100        110        120 
DEAAITAAMA GAQRALGPLT GLVNNAGIAG FGSVHATEVE TWSRIMAVNV TGTFLASKAA 

       130        140        150        160        170        180 
LFGMLERGRG AIVNFGSVAG LVGIPTMAAY CAAKGAVVNL TRQMAADYSG RGIRVNVVCP 

       190        200        210        220        230        240 
GTVAGTDMGR QLLGTDCDPE LEARRLAKYP MGRFGTPEDI AEAAVFLLST KAAFVTGSVL 


AVDGGMTAI 

« Hide

References

« Hide 'large scale' references
[1]"Complementation of Xanthobacter Py2 mutants in epoxyalkane degradation; expression and nucleotide sequence of the complementing DNA fragment."
Swaving J., Weijers C.A.G.M., van Ooyen A.J.J., de Bont J.A.M.
Microbiology 141:477-484(1995) [PubMed: 7704278] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete sequence of plasmid pXAUT01 of Xanthobacter autotrophicus Py2."
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Brainard J., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Ensigns S.A., Richardson P.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Purification to homogeneity and reconstitution of the individual components of the epoxide carboxylase multiprotein enzyme complex from Xanthobacter strain Py2."
Allen J.R., Ensign S.A.
J. Biol. Chem. 272:32121-32128(1997) [PubMed: 9405410] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-10, CHARACTERIZATION.
[4]"Aliphatic epoxide carboxylation."
Ensign S.A., Allen J.R.
Annu. Rev. Biochem. 72:55-76(2003) [PubMed: 12524213] [Abstract]
Cited for: REVIEW.

Cross-references

Sequence databases

X79863 Genomic DNA. Translation: CAA56245.1.
CP000782 Genomic DNA. Translation: ABS70080.1.
PIRS47055.
RefSeqYP_001409316.1.

3D structure databases

HSSPHSSP built from PDB template 1G0N based on UniProtKB Q12634.
ModBaseSearch...

Genome annotation databases

GeneID5420357.
GenomeReviewsGene locus Xaut_4869 in contig CP000782_GR.
KEGGxau:Xaut_4869.

Organism-specific databases

CMRSearch...

Enzyme and pathway databases

BioCycMetaCyc:MON-13284.

Family and domain databases

InterProIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHERPTHR19410. ADH_short_C2. 1 hit.
PfamPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHCDS_XANP2
AccessionPrimary (citable) accession number: Q56841
Secondary accession number(s): A7IPY4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: November 1, 1996
Last modified: June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents