Q56841 (HCDS_XANP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-(S)-hydroxypropyl-CoM dehydrogenase
Short name=S-HPCDH
EC=1.1.1.269

Alternative name(s):
Aliphatic epoxide carboxylation component IV

Gene names

Name:	xecE
Ordered Locus Names:	Xaut_4869

Encoded on

Plasmid pXAUT01

Organism

Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) [Complete proteome] [HAMAP]

Taxonomic identifier

78245 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Xanthobacteraceae › Xanthobacter

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the oxidation of 2-(S)-hydroxyalkyl thioesters of CoM to 2-oxoalkyl thioesters of CoM. The enzyme highly specific for the S enantiomers.
Catalytic activity	2-(S)-hydroxypropyl-CoM + NAD+ = 2-oxopropyl-CoM + NADH.
Pathway	Alkene metabolism; propylene degradation.
Subunit structure	Homodimer. Component IV of the aliphatic epoxide carboxylation complex together with components I, II and III.
Induction	By aliphatic and chlorinated alkenes.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Plasmid
Gene Ontology (GO)
Molecular function	2-(S)-hydroxypropyl-CoM dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	2-(S)-hydroxypropyl-CoM dehydrogenase		PRO_0000054706
Sites
	Active site	150	1	Proton acceptor By similarity
	Binding site	137	1	Substrate By similarity
Experimental info
	Sequence conflict	4	1	A → N AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q56841 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: B5E0B82C1D8D9782	FASTA	249	24,941
	10 20 30 40 50 60 MLDAEVIAIT GGAAGIGLAV AHAAIRAGAR VALIDRDGAC AQRAAAEFGA AAWGVGADVT 70 80 90 100 110 120 DEAAITAAMA GAQRALGPLT GLVNNAGIAG FGSVHATEVE TWSRIMAVNV TGTFLASKAA 130 140 150 160 170 180 LFGMLERGRG AIVNFGSVAG LVGIPTMAAY CAAKGAVVNL TRQMAADYSG RGIRVNVVCP 190 200 210 220 230 240 GTVAGTDMGR QLLGTDCDPE LEARRLAKYP MGRFGTPEDI AEAAVFLLST KAAFVTGSVL AVDGGMTAI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complementation of Xanthobacter Py2 mutants in epoxyalkane degradation; expression and nucleotide sequence of the complementing DNA fragment." Swaving J., Weijers C.A.G.M., van Ooyen A.J.J., de Bont J.A.M. Microbiology 141:477-484(1995) [PubMed: 7704278] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of plasmid pXAUT01 of Xanthobacter autotrophicus Py2." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Brainard J., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Ensigns S.A., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1158 / Py2.
[3]	"Purification to homogeneity and reconstitution of the individual components of the epoxide carboxylase multiprotein enzyme complex from Xanthobacter strain Py2." Allen J.R., Ensign S.A. J. Biol. Chem. 272:32121-32128(1997) [PubMed: 9405410] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-10, CHARACTERIZATION.
[4]	"Aliphatic epoxide carboxylation." Ensign S.A., Allen J.R. Annu. Rev. Biochem. 72:55-76(2003) [PubMed: 12524213] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X79863 Genomic DNA. Translation: CAA56245.1. CP000782 Genomic DNA. Translation: ABS70080.1.
PIR	S47055.
RefSeq	YP_001409316.1. NC_009717.1.
3D structure databases
ProteinModelPortal	Q56841.
ModBase	Search...
Protein-protein interaction databases
STRING	Q56841.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5420357.
GenomeReviews	Gene locus Xaut_4869 in contig CP000782_GR.
KEGG	xau:Xaut_4869.
PATRIC	24041519. VBIXanAut29526_0071.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1028.
HOGENOM	HBG750976.
ProtClustDB	CLSK979092.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-13284. XAUT78245:XAUT_4869-MONOMER.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HCDS_XANP2

Accession

Primary (citable) accession number: Q56841
Secondary accession number(s): A7IPY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program