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Protein

2-(S)-hydroxypropyl-CoM dehydrogenase

Gene

xecE

Organism
Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of 2-(S)-hydroxyalkyl thioesters of CoM to 2-oxoalkyl thioesters of CoM. The enzyme highly specific for the S enantiomers.

Catalytic activityi

2-(S)-hydroxypropyl-CoM + NAD+ = 2-oxopropyl-CoM + NADH.

Pathwayi: propylene degradation

This protein is involved in the pathway propylene degradation, which is part of Alkene metabolism.
View all proteins of this organism that are known to be involved in the pathway propylene degradation and in Alkene metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei137 – 1371SubstrateBy similarity
Active sitei150 – 1501Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13284.
XAUT78245:GHS6-4928-MONOMER.
BRENDAi1.1.1.269. 1641.
UniPathwayiUPA00776.

Names & Taxonomyi

Protein namesi
Recommended name:
2-(S)-hydroxypropyl-CoM dehydrogenase (EC:1.1.1.269)
Short name:
S-HPCDH
Alternative name(s):
Aliphatic epoxide carboxylation component IV
Gene namesi
Name:xecE
Ordered Locus Names:Xaut_4869
Encoded oniPlasmid pXAUT010 Publication
OrganismiXanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)
Taxonomic identifieri78245 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeXanthobacter
Proteomesi
  • UP000002417 Componenti: Plasmid pXAUT01

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2492492-(S)-hydroxypropyl-CoM dehydrogenasePRO_0000054706Add
BLAST

Expressioni

Inductioni

By aliphatic and chlorinated alkenes.

Interactioni

Subunit structurei

Homodimer. Component IV of the aliphatic epoxide carboxylation complex together with components I, II and III.

Structurei

3D structure databases

ProteinModelPortaliQ56841.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

OrthoDBiEOG6N3CR8.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q56841-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDAEVIAIT GGAAGIGLAV AHAAIRAGAR VALIDRDGAC AQRAAAEFGA
60 70 80 90 100
AAWGVGADVT DEAAITAAMA GAQRALGPLT GLVNNAGIAG FGSVHATEVE
110 120 130 140 150
TWSRIMAVNV TGTFLASKAA LFGMLERGRG AIVNFGSVAG LVGIPTMAAY
160 170 180 190 200
CAAKGAVVNL TRQMAADYSG RGIRVNVVCP GTVAGTDMGR QLLGTDCDPE
210 220 230 240
LEARRLAKYP MGRFGTPEDI AEAAVFLLST KAAFVTGSVL AVDGGMTAI
Length:249
Mass (Da):24,941
Last modified:November 1, 1996 - v1
Checksum:iB5E0B82C1D8D9782
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41A → N AA sequence (PubMed:9405410).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79863 Genomic DNA. Translation: CAA56245.1.
CP000782 Genomic DNA. Translation: ABS70080.1.
PIRiS47055.
RefSeqiWP_011992984.1. NC_009717.1.

Genome annotation databases

EnsemblBacteriaiABS70080; ABS70080; Xaut_4869.
KEGGixau:Xaut_4869.
PATRICi24041519. VBIXanAut29526_0071.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79863 Genomic DNA. Translation: CAA56245.1.
CP000782 Genomic DNA. Translation: ABS70080.1.
PIRiS47055.
RefSeqiWP_011992984.1. NC_009717.1.

3D structure databases

ProteinModelPortaliQ56841.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS70080; ABS70080; Xaut_4869.
KEGGixau:Xaut_4869.
PATRICi24041519. VBIXanAut29526_0071.

Phylogenomic databases

OrthoDBiEOG6N3CR8.

Enzyme and pathway databases

UniPathwayiUPA00776.
BioCyciMetaCyc:MONOMER-13284.
XAUT78245:GHS6-4928-MONOMER.
BRENDAi1.1.1.269. 1641.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complementation of Xanthobacter Py2 mutants in epoxyalkane degradation; expression and nucleotide sequence of the complementing DNA fragment."
    Swaving J., Weijers C.A.G.M., van Ooyen A.J.J., de Bont J.A.M.
    Microbiology 141:477-484(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1158 / Py2.
  3. "Purification to homogeneity and reconstitution of the individual components of the epoxide carboxylase multiprotein enzyme complex from Xanthobacter strain Py2."
    Allen J.R., Ensign S.A.
    J. Biol. Chem. 272:32121-32128(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-10, CHARACTERIZATION.
  4. Cited for: REVIEW.

Entry informationi

Entry nameiHCDS_XANP2
AccessioniPrimary (citable) accession number: Q56841
Secondary accession number(s): A7IPY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Plasmid, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.