Reviewed, UniProtKB/Swiss-Prot Q56806 (PEL_XANCM)

Names and origin

Protein names	Recommended name:     Pectate lyase       Short name=PL     EC=4.2.2.2 Alternative name(s):     PSTRU-3
Organism	Xanthomonas campestris pv. malvacearum
Taxonomic identifier	86040 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays a role in bacterial invasion of plants.
Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Subcellular location	Secreted.
Sequence similarities	Belongs to the polysaccharide lyase 1 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 377	351	Pectate lyase		PRO_0000024864
Sites
	Active site	262	1	Potential
	Metal binding	166	1	Calcium By similarity
	Metal binding	205	1	Calcium By similarity
	Metal binding	209	1	Calcium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q56806-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A947A0D9BF5A81A7	FASTA	377	40,184
	10 20 30 40 50 60 MKPKFSTAAA ASLFVGSLLV VGVACADPAL EVATTGWATQ NGGTKGGSKA AAANIYTVKT 70 80 90 100 110 120 AAELKSALKA SVGSNGRIIK ISGIIDVSEG KPYTTTSDMK SRARLDIPTK TTLIGITSNA 130 140 150 160 170 180 EIREGYFYVK ANDVIIRNIT IENPWDPEPV WDPDDGSAGN WNSEYDGLTV EGATNVWVDH 190 200 210 220 230 240 VTFTDGRRTD DQNGTANGRP KQHHDGAMDV KKGANFVTIS YSAFKSHEKN DLIGSSDSAS 250 260 270 280 290 300 STDSGKLKVT IHNTLFENIS ARAPRVRFGQ VHLYNNYHVG STSNKVYPFS HAHGVGKESK 310 320 330 340 350 360 IFSERNVFDI SGVSSCDKIA ADYGGSVYRD QGSLLNGKAL TCSWNSNIGW TPPYTYSLLS 370 ADKVAADVKA KAGAGKL

References

[1]

"Cloning of a pectate lyase gene from Xanthomonas campestris pv. malvacearum and comparison of its sequence relationship with pel genes of soft-rot Erwinia and Pseudomonas." Liao C.H., Gaffney T.D., Bradley S.P., Wong L.C. Mol. Plant Microbe Interact. 9:14-21(1996) [PubMed: 8589419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B-414.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L38573 Genomic DNA. Translation: AAC41522.1.
3D structure databases
SMR	Q56806. Positions 26-377.
ModBase	Search...
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
SMART	SM00656. Amb_all. 1 hit. SM00710. PbH1. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEL_XANCM

Accession

Primary (citable) accession number: Q56806

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)