Q567B1 (OTU1_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin thioesterase OTU1
EC=3.4.19.12

Gene names

Name:	yod1
ORF Names:	zgc:112182

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Otocephala › Ostariophysi › Otophysi › Cypriniphysi › Cypriniformes › Cyprinoidea › Cyprinidae › Danio

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Contains 1 C2H2-type zinc finger. Contains 1 OTU domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway Unfolded protein response
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER-associated protein catabolic process Inferred from sequence or structural similarity. Source: UniProtKB cellular amino acid metabolic process Inferred from sequence or structural similarity. Source: UniProtKB endoplasmic reticulum unfolded protein response Inferred from sequence or structural similarity. Source: UniProtKB protein K48-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB protein K63-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	Ubiquitin thioesterase OTU1		PRO_0000282360
Regions
	Domain	102 – 227	126	OTU
	Zinc finger	271 – 295	25	C2H2-type
	Region	5 – 83	79	UBX-like
Sites
	Active site	110	1	By similarity
	Active site	113	1	Nucleophile By similarity
	Active site	295	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q567B1 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 70D85A2694B56198	FASTA	301	33,486
	10 20 30 40 50 60 MLRLRCKAKN GTHLMQGLTH QSCVQELKDK IEELTGIPCD VQKIMVGYPP SSLDLRNGEA 70 80 90 100 110 120 HLKDYPIKSG DTLIVEEEKN KPKPPVQPTV TKGPSFEVTP VVERRVVPAD NSCLFTSVNY 130 140 150 160 170 180 VMEGGVYDPA CASEMRGLIA QIVASDPTAY SEAVLGKTNE EYCTWIRRDD TWGGAIEVSI 190 200 210 220 230 240 LSKFYQCEIC VVDTQTVRVD RFGEDAGYTK RVLLIYDGIH YDPLQKVLPG SDVPAQTVFS 250 260 270 280 290 300 TVDDVILAQA LELADEARRK RQFTDVNRFA LRCMVCQTGL VGQKEAREHA KETGHTNFGE V

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC093247 mRNA. Translation: AAH93247.1.
IPI	IPI00481398.
RefSeq	NP_001017716.1. NM_001017716.2.
UniGene	Dr.104832.
3D structure databases
ProteinModelPortal	Q567B1.
ModBase	Search...
Protein-protein interaction databases
STRING	7955.ENSDARP00000003565.
Protein family/group databases
MEROPS	C85.007.
Proteomic databases
PRIDE	Q567B1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000023388; ENSDARP00000003565; ENSDARG00000008542.
GeneID	550411.
KEGG	dre:550411.
Organism-specific databases
CTD	55432.
ZFIN	ZDB-GENE-050417-217. yod1.
Phylogenomic databases
eggNOG	COG5539.
GeneTree	ENSGT00390000009989.
HOGENOM	HOG000193461.
HOVERGEN	HBG097006.
InParanoid	Q567B1.
KO	K13719.
OMA	PIQSGDM.
OrthoDB	EOG4GF3FR.
Gene expression databases
ArrayExpress	Q567B1.
Bgee	Q567B1.
Family and domain databases
InterPro	IPR003323. OTU. IPR000626. Ubiquitin. IPR019955. Ubiquitin_supergroup. [Graphical view]
Pfam	PF02338. OTU. 1 hit. [Graphical view]
SMART	SM00213. UBQ. 1 hit. [Graphical view]
PROSITE	PS50802. OTU. 1 hit. PS00299. UBIQUITIN_1. False negative. PS50053. UBIQUITIN_2. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 1 hit. PS50157. ZINC_FINGER_C2H2_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	20879660.

Entry information

Entry name

OTU1_DANRE

Accession

Primary (citable) accession number: Q567B1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	May 10, 2005
Last modified:	April 3, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program