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Q567B1 (OTU1_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ubiquitin thioesterase OTU1

EC=3.4.19.12
Gene names
Name:yod1
ORF Names:zgc:112182
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length301 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin chain on the associated substrate to facilitate their threading through the VCP/p97 pore. Ubiquitin moieties on substrates may present a steric impediment to the threading process when the substrate is transferred to the VCP pore and threaded through VCP's axial channel. Mediates deubiquitination of 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains. Also able to hydrolyze 'Lys-11'-linked ubiquitin chains. Cleaves both polyubiquitin and di-ubiquitin By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Domain

The C2H2-type zinc finger mediates specificity for 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains but not for 'Lys-11'-linked ubiquitin chains. Selectivity for 'Lys-11'-linked ubiquitin chains is provided by recognition of the sequence surrounding 'Lys-11' in ubiquitin. The S2 site region provides specificity for longer 'Lys-11'-linked ubiquitin chains By similarity.

Sequence similarities

Contains 1 C2H2-type zinc finger.

Contains 1 OTU domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 301301Ubiquitin thioesterase OTU1
PRO_0000282360

Regions

Domain102 – 227126OTU
Zinc finger271 – 29525C2H2-type
Region5 – 8379UBX-like
Region107 – 1137Cys-loop By similarity
Region166 – 17611Variable-loop By similarity
Region216 – 2205His-loop By similarity
Region244 – 2496S2 site By similarity

Sites

Active site1101 By similarity
Active site1131Nucleophile By similarity
Active site2201 By similarity
Binding site2191Substrate; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q567B1 [UniParc].

Last modified May 10, 2005. Version 1.
Checksum: 70D85A2694B56198

FASTA30133,486
        10         20         30         40         50         60 
MLRLRCKAKN GTHLMQGLTH QSCVQELKDK IEELTGIPCD VQKIMVGYPP SSLDLRNGEA 

        70         80         90        100        110        120 
HLKDYPIKSG DTLIVEEEKN KPKPPVQPTV TKGPSFEVTP VVERRVVPAD NSCLFTSVNY 

       130        140        150        160        170        180 
VMEGGVYDPA CASEMRGLIA QIVASDPTAY SEAVLGKTNE EYCTWIRRDD TWGGAIEVSI 

       190        200        210        220        230        240 
LSKFYQCEIC VVDTQTVRVD RFGEDAGYTK RVLLIYDGIH YDPLQKVLPG SDVPAQTVFS 

       250        260        270        280        290        300 
TVDDVILAQA LELADEARRK RQFTDVNRFA LRCMVCQTGL VGQKEAREHA KETGHTNFGE 


V 

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References

[1]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC093247 mRNA. Translation: AAH93247.1.
RefSeqNP_001017716.1. NM_001017716.2.
UniGeneDr.104832.

3D structure databases

ProteinModelPortalQ567B1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7955.ENSDARP00000003565.

Protein family/group databases

MEROPSC85.007.

Proteomic databases

PRIDEQ567B1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSDART00000023388; ENSDARP00000003565; ENSDARG00000008542.
GeneID550411.
KEGGdre:550411.

Organism-specific databases

CTD55432.
ZFINZDB-GENE-050417-217. yod1.

Phylogenomic databases

eggNOGCOG5539.
GeneTreeENSGT00390000009989.
HOGENOMHOG000193461.
HOVERGENHBG097006.
InParanoidQ567B1.
KOK13719.
OMAWIRREET.
OrthoDBEOG7J1808.
PhylomeDBQ567B1.
TreeFamTF323700.

Gene expression databases

BgeeQ567B1.

Family and domain databases

InterProIPR003323. OTU.
IPR000626. Ubiquitin-like.
[Graphical view]
PfamPF02338. OTU. 1 hit.
PF14560. Ubiquitin_2. 1 hit.
[Graphical view]
SMARTSM00213. UBQ. 1 hit.
[Graphical view]
PROSITEPS50802. OTU. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20879660.
PROQ567B1.

Entry information

Entry nameOTU1_DANRE
AccessionPrimary (citable) accession number: Q567B1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: May 10, 2005
Last modified: April 16, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families