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Protein

Type 4 prepilin-like proteins leader peptide-processing enzyme

Gene

xpsO

Organism
Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue.By similarity

Catalytic activityi

Typically cleaves a -Gly-|-Phe- bond to release an N-terminal, basic peptide of 5-8 residues from type IV prepilin, and then N-methylates the new N-terminal amino group, the methyl donor being S-adenosyl-L-methionine.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Methyltransferase, Protease, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciXCAM190485:GIXZ-3099-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Type 4 prepilin-like proteins leader peptide-processing enzyme
Including the following 2 domains:
Leader peptidase (EC:3.4.23.43)
Alternative name(s):
Prepilin peptidase
N-methyltransferase (EC:2.1.1.-)
Gene namesi
Name:xpsO
Synonyms:pilD
Ordered Locus Names:XCC3101
OrganismiXanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)
Taxonomic identifieri190485 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
Proteomesi
  • UP000001010 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei10 – 3021HelicalSequence analysisAdd
BLAST
Transmembranei101 – 12121HelicalSequence analysisAdd
BLAST
Transmembranei125 – 14521HelicalSequence analysisAdd
BLAST
Transmembranei177 – 19721HelicalSequence analysisAdd
BLAST
Transmembranei226 – 24621HelicalSequence analysisAdd
BLAST
Transmembranei253 – 27321HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 287287Type 4 prepilin-like proteins leader peptide-processing enzymePRO_0000192631Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi190485.XCC3101.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A24 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EHH. Bacteria.
COG1989. LUCA.
HOGENOMiHOG000248584.
KOiK02654.
OMAiVFWLFKL.
OrthoDBiEOG6F55M8.

Family and domain databases

InterProiIPR010627. Pept_A24A_N.
IPR014032. Peptidase_A24A_bac.
IPR000045. Prepilin_IV_endopep_pep.
[Graphical view]
PfamiPF06750. DiS_P_DiS. 1 hit.
PF01478. Peptidase_A24. 1 hit.
[Graphical view]
PRINTSiPR00864. PREPILNPTASE.

Sequencei

Sequence statusi: Complete.

Q56763-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFLDQHPGL GFPAAAGLGL LIGSFLNVVI LRLPKRMEWQ WRRDAREILE
60 70 80 90 100
LPDIYEPPPP GIVVEPSHDP VTGDKLKWWE NIPLFSWLML RGKSRYSGKP
110 120 130 140 150
ISIQYPLVEL LTSILCVASV WRFGFGWQGF GAIVLSCFLV AMSGIDLRHK
160 170 180 190 200
LLPDQLTLPL MWLGLVGSMD NLYMPAKPAL LGAAVGYVSL WTVWWLFKQL
210 220 230 240 250
TGKEGMGHGD FKLLAALGAW CGLKGILPII LISSLVGAVL GSIWLFAKGR
260 270 280
DRATPIPFGP YLAIAGWVVF FWGNDLVDGY LRFAGLR
Length:287
Mass (Da):31,843
Last modified:November 1, 1996 - v1
Checksum:iA58DD1A514F9ECC2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12432 Genomic DNA. Translation: AAC43571.1.
AE008922 Genomic DNA. Translation: AAM42372.1.
PIRiS70810.
RefSeqiNP_638448.1. NC_003902.1.
WP_011038216.1. NC_003902.1.

Genome annotation databases

EnsemblBacteriaiAAM42372; AAM42372; XCC3101.
GeneIDi998351.
KEGGixcc:XCC3101.
PATRICi24077250. VBIXanCam115730_3313.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12432 Genomic DNA. Translation: AAC43571.1.
AE008922 Genomic DNA. Translation: AAM42372.1.
PIRiS70810.
RefSeqiNP_638448.1. NC_003902.1.
WP_011038216.1. NC_003902.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi190485.XCC3101.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM42372; AAM42372; XCC3101.
GeneIDi998351.
KEGGixcc:XCC3101.
PATRICi24077250. VBIXanCam115730_3313.

Phylogenomic databases

eggNOGiENOG4105EHH. Bacteria.
COG1989. LUCA.
HOGENOMiHOG000248584.
KOiK02654.
OMAiVFWLFKL.
OrthoDBiEOG6F55M8.

Enzyme and pathway databases

BioCyciXCAM190485:GIXZ-3099-MONOMER.

Family and domain databases

InterProiIPR010627. Pept_A24A_N.
IPR014032. Peptidase_A24A_bac.
IPR000045. Prepilin_IV_endopep_pep.
[Graphical view]
PfamiPF06750. DiS_P_DiS. 1 hit.
PF01478. Peptidase_A24. 1 hit.
[Graphical view]
PRINTSiPR00864. PREPILNPTASE.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The type IV pre-pilin leader peptidase of Xanthomonas campestris pv. campestris is functional without conserved cysteine residues."
    Hu N.-T.T., Lee P.F., Chen C.
    Mol. Microbiol. 18:769-777(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Xc1701.
  2. "Comparison of the genomes of two Xanthomonas pathogens with differing host specificities."
    da Silva A.C.R., Ferro J.A., Reinach F.C., Farah C.S., Furlan L.R., Quaggio R.B., Monteiro-Vitorello C.B., Van Sluys M.A., Almeida N.F. Jr., Alves L.M.C., do Amaral A.M., Bertolini M.C., Camargo L.E.A., Camarotte G., Cannavan F., Cardozo J., Chambergo F., Ciapina L.P.
    , Cicarelli R.M.B., Coutinho L.L., Cursino-Santos J.R., El-Dorry H., Faria J.B., Ferreira A.J.S., Ferreira R.C.C., Ferro M.I.T., Formighieri E.F., Franco M.C., Greggio C.C., Gruber A., Katsuyama A.M., Kishi L.T., Leite R.P., Lemos E.G.M., Lemos M.V.F., Locali E.C., Machado M.A., Madeira A.M.B.N., Martinez-Rossi N.M., Martins E.C., Meidanis J., Menck C.F.M., Miyaki C.Y., Moon D.H., Moreira L.M., Novo M.T.M., Okura V.K., Oliveira M.C., Oliveira V.R., Pereira H.A., Rossi A., Sena J.A.D., Silva C., de Souza R.F., Spinola L.A.F., Takita M.A., Tamura R.E., Teixeira E.C., Tezza R.I.D., Trindade dos Santos M., Truffi D., Tsai S.M., White F.F., Setubal J.C., Kitajima J.P.
    Nature 417:459-463(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25.

Entry informationi

Entry nameiLEP4_XANCP
AccessioniPrimary (citable) accession number: Q56763
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: November 11, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.