Q56725 (NHAA_VIBPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Na(+)/H(+) antiporter nhaA

Alternative name(s):
Sodium/proton antiporter nhaA

Gene names

Name:	nhaA
Ordered Locus Names:	VP1228

Organism

Vibrio parahaemolyticus [Complete proteome] [HAMAP]

Taxonomic identifier

670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Na+/H+ antiporter that extrudes sodium in exchange for external protons. Activity is pH-dependent. Ref.1
Enzyme regulation	Amiloride strongly reduces affinity for Na+, but does not change the Vmax. Ref.1
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_01844.
Sequence similarities	Belongs to the nhaA Na(+)/H(+) (TC 2.A.33) antiporter family. [View classification]
Biophysicochemical properties	Kinetic parameters: KM=36 mM for Na+ Ref.1 KM=24 mM for Li+ pH dependence: Optimum pH is 8.5. Activity is null at pH 7.0 and increases as the pH increases from 7.0 to 8.5.

Ontologies

Keywords
Biological process	Antiport Ion transport Sodium transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Sodium
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of pH Inferred from electronic annotation. Source: InterPro sodium ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	antiporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 383	383	Na(+)/H(+) antiporter nhaA HAMAP MF_01844		PRO_0000334460
Regions
	Transmembrane	14 – 34	21	Helical; Potential
	Transmembrane	47 – 67	21	Helical; Potential
	Transmembrane	87 – 107	21	Helical; Potential
	Transmembrane	117 – 137	21	Helical; Potential
	Transmembrane	146 – 166	21	Helical; Potential
	Transmembrane	171 – 191	21	Helical; Potential
	Transmembrane	205 – 225	21	Helical; Potential
	Transmembrane	252 – 272	21	Helical; Potential
	Transmembrane	280 – 300	21	Helical; Potential
	Transmembrane	321 – 341	21	Helical; Potential
	Transmembrane	356 – 376	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q56725 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 9EE53D008D2FD6A2	FASTA	383	40,433
	10 20 30 40 50 60 MNDVIRDFFK MESAGGILLV IAAAIAMTIA NSPLGETYQS LLHTYVFGMS VSHWINDGLM 70 80 90 100 110 120 AVFFLLIGLE VKRELLEGAL KSKETAIFPA IAAVGGMLAP ALIYVAFNAN DPEAISGWAI 130 140 150 160 170 180 PAATDIAFAL GIMALLGKRV PVSLKVFLLA LAIIDDLGVV VIIALFYTGD LSSMALLVGF 190 200 210 220 230 240 VMTGVLFMLN AKEVTKLTPY MIVGAILWFA VLKSGVHATL AGVVIGFAIP LKGKQGEHSP 250 260 270 280 290 300 LKHMEHALHP YVAFGILPLF AFANAGISLE GVSMSGLTSM LPLGIALGLL IGKPLGIFSF 310 320 330 340 350 360 SWAAVKLGVA KLPEGINFKH IFAVSVLCGI GFTMSIFISS LAFGNVSPEF DTYARLGILM 370 380 GSTTAAVLGY ALLHFSLPKK AQD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Properties and sequence of the NhaA Na+/H+ antiporter of Vibrio parahaemolyticus." Kuroda T., Shimamoto T., Inaba K., Tsuda M., Tsuchiya T. J. Biochem. 116:1030-1038(1994) [PubMed: 7896730] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES. Strain: AQ3334.
[2]	"Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae." Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T. Lancet 361:743-749(2003) [PubMed: 12620739] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RIMD 2210633 / Serotype O3:K6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D28504 Genomic DNA. Translation: BAA05864.1. BA000031 Genomic DNA. Translation: BAC59491.1.
PIR	JX0360.
RefSeq	NP_797607.1. NC_004603.1.
3D structure databases
HSSP	HSSP built from PDB template 1ZCD based on UniProtKB P13738.
ProteinModelPortal	Q56725.
SMR	Q56725. Positions 11-377.
ModBase	Search...
Protein family/group databases
TCDB	2.A.33.1.2. NhaA Na+:H+ antiporter (NhaA) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1188733.
GenomeReviews	Gene locus VP1228 in contig BA000031_GR.
KEGG	vpa:VP1228.
NMPDR	fig|223926.1.peg.1228.
PATRIC	20140662. VBIVibPar50997_1168.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG665619.
OMA	LIHWIND.
ProtClustDB	PRK09560.
Enzyme and pathway databases
BioCyc	VPAR223926:VP1228-MONOMER.
Family and domain databases
HAMAP	MF_01844. NhaA. [Tree]
InterPro	IPR023171. Na/H_antiporter_dom. IPR004670. NhaA. [Graphical view]
Gene3D	G3DSA:1.20.1530.10. G3DSA:1.20.1530.10. 1 hit.
KO	K03313.
Pfam	PF06965. Na_H_antiport_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00773. NhaA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NHAA_VIBPA

Accession

Primary (citable) accession number: Q56725

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program