Q566U4 (AFMID_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable arylformamidase
EC=3.5.1.9

Alternative name(s):
Kynurenine formamidase
Short name=KF

Gene names

Name:	afmid
ORF Names:	zgc:112472

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the conversion of tryptophan to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites By similarity.
Catalytic activity	N-formyl-L-kynurenine + H2O = formate + L-kynurenine.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 2/2.
Subcellular location	Cytoplasm › cytosol By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the AFMID family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tryptophan catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	arylformamidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q566U4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Gene prediction based on EST data.

Isoform 2 (identifier: Q566U4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-24: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 293	293	Probable arylformamidase		PRO_0000361878
Sites
	Active site	153	1	By similarity
	Active site	236	1	By similarity
	Active site	268	1	By similarity
Natural variations
	Alternative sequence	1 – 24	24	Missing in isoform 2.		VSP_036236

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 10, 2009. Version 2. Checksum: C2617B74DB3F65E1	FASTA	293	32,412
	10 20 30 40 50 60 MSDWKSMSKD ELELQYSPSR WSPRMSADDV IRAHVSALKS GTERARSVTQ TLMDVPYGEA 70 80 90 100 110 120 EGEKLDVYLP SSSSPDVPLV IYFHGGYWQF LSKDESGFLA VPLVQKGAVV VAVGYSIAPK 130 140 150 160 170 180 GDMDLMVSQV RRSVVSVIQQ YSHISGLYLC GHSAGAHLAA MVLSTDWTQY DVSPKIKGAF 190 200 210 220 230 240 LVSGIYDLQP ILSTYVNEPL KMTEEVALRN SPSRFLSQLK VSSASCDITV AVAQNDSPEF 250 260 270 280 290 RKQSEEYYKA LESAGLKVSF EDVSETDHFS IIEQLVDENY LLTKLLLKMI GKS
	Isoform 2 [UniParc]. Checksum: A2393E5041F29B23	FASTA	269	29,486
	10 20 30 40 50 60 MSADDVIRAH VSALKSGTER ARSVTQTLMD VPYGEAEGEK LDVYLPSSSS PDVPLVIYFH 70 80 90 100 110 120 GGYWQFLSKD ESGFLAVPLV QKGAVVVAVG YSIAPKGDMD LMVSQVRRSV VSVIQQYSHI 130 140 150 160 170 180 SGLYLCGHSA GAHLAAMVLS TDWTQYDVSP KIKGAFLVSG IYDLQPILST YVNEPLKMTE 190 200 210 220 230 240 EVALRNSPSR FLSQLKVSSA SCDITVAVAQ NDSPEFRKQS EEYYKALESA GLKVSFEDVS 250 260 ETDHFSIIEQ LVDENYLLTK LLLKMIGKS

References

[1]	The Danio rerio sequencing project at the Sanger Institute Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Olfactory epithelium.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR293511 Genomic DNA. No translation available. BC093330 mRNA. Translation: AAH93330.1.
IPI	IPI00483797. IPI00852089.
RefSeq	NP_001017677.1. NM_001017677.1.
UniGene	Dr.82584.
3D structure databases
ProteinModelPortal	Q566U4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q566U4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	550372.
KEGG	dre:550372.
Organism-specific databases
CTD	125061.
ZFIN	ZDB-GENE-050417-166. afmid.
Phylogenomic databases
eggNOG	fiNOG10950.
GeneTree	ENSGT00390000011093.
HOGENOM	HBG714368.
HOVERGEN	HBG100436.
OrthoDB	EOG470THR.
Gene expression databases
Bgee	Q566U4.
Family and domain databases
InterPro	IPR013094. AB_hydrolase_3. [Graphical view]
KO	K01432.
Pfam	PF07859. Abhydrolase_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AFMID_DANRE

Accession

Primary (citable) accession number: Q566U4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	February 10, 2009
Last modified:	November 16, 2011
This is version 42 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program