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Protein

Atypical kinase COQ8B, mitochondrial

Gene

Coq8b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Atypical kinase involved in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration. Its substrate specificity is unclear: does not show any protein kinase activity. Probably acts as a small molecule kinase, possibly a lipid kinase that phosphorylates a prenyl lipid in the ubiquinone biosynthesis pathway. Required for podocyte migration.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei220ATPBy similarity1
Binding sitei238ATPBy similarity1
Active sitei368Proton acceptorBy similarity1
Binding sitei373ATPBy similarity1
Binding sitei387ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi325 – 328ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Atypical kinase COQ8B, mitochondrialCurated (EC:2.7.-.-By similarity)
Alternative name(s):
AarF domain-containing protein kinase 4Imported
Coenzyme Q protein 8BBy similarity
Gene namesi
Name:Coq8bBy similarity
Synonyms:Adck4Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1924139. Adck4.

Subcellular locationi

  • Mitochondrion membrane By similarity; Single-pass membrane protein By similarity
  • Cytoplasmcytosol By similarity
  • Cell membrane By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei93 – 109HelicalSequence analysisAdd BLAST17

GO - Cellular componenti

  • cytosol Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial membrane Source: UniProtKB-SubCell
  • mitochondrion Source: MGI
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002717981 – 533Atypical kinase COQ8B, mitochondrialAdd BLAST533

Proteomic databases

EPDiQ566J8.
MaxQBiQ566J8.
PaxDbiQ566J8.
PRIDEiQ566J8.

PTM databases

PhosphoSitePlusiQ566J8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000003762.
CleanExiMM_ADCK4.

Interactioni

Subunit structurei

Homodimer; homodimerizes via its transmembrane region. Interacts with COQ6 and COQ7. Interacts with the multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000003860.

Structurei

3D structure databases

ProteinModelPortaliQ566J8.
SMRiQ566J8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini192 – 424Protein kinaseAdd BLAST233

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi156 – 159KxGQ motifBy similarity4
Motifi217 – 220AAAS motifBy similarity4

Domaini

Adopts an atypical protein kinase-like fold: while it adopts a core fold similar to that of well-characterized protein kinase-like domains. The KxGQ motif completely occludes the typical substrate binding pocket. Nucleotide-binding opens the substrate binding pocket and flips the active site from inside the hydrophobic core into a catalytically competent, solvent-exposed posture.By similarity

Sequence similaritiesi

Contains 1 protein kinase domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1234. Eukaryota.
COG0661. LUCA.
HOGENOMiHOG000201140.
HOVERGENiHBG061318.
InParanoidiQ566J8.
PhylomeDBiQ566J8.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR004147. UbiB_dom.
[Graphical view]
PfamiPF03109. ABC1. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.

Sequencei

Sequence statusi: Complete.

Q566J8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWLELGAMLR RTCGPLGRAV RLPCGGALGP RPHWWGPCRS CLAQSVHQDQ
60 70 80 90 100
PGRGLSEDDI RRAREARLRK APRPQLSDRS RERKVPASRI SRLASFGGLA
110 120 130 140 150
VGLGLGALAE VTKKSLPGGS LQHEGVSGLG SSPFLSEANA ERIVQTLCTV
160 170 180 190 200
RGAALKIGQM LSIQDNSFIS PQLQRIFERV RQSADFMPRW QMMRVLEEEL
210 220 230 240 250
GKDWQDKVAS LEEVPFAAAS IGQVHQGLLK DGTEVAVKIQ YPGVAQSIQS
260 270 280 290 300
DVENLLALLK MSVGLPEGLF AEQSLQTLQQ ELAWECDYRR EAACAQTFRK
310 320 330 340 350
LLADDPFFRV PAVVQELCTT RVLGMELAGG IPLDQCQGLS QDIRNQICFQ
360 370 380 390 400
LLRLCLRELF EFRFMQTDPN WANFLYDASS HQVTLLDFGA SRAFGTEFTD
410 420 430 440 450
HYIEVVKAAA DGDRDRVLQK SQDLKFLTGF ETKAFSDAHV EAVMILGEPF
460 470 480 490 500
AASGPYDFGA GETARRIQGL IPVLLRHRLR PPPEETYALH RKLAGAFLAC
510 520 530
ARLHAHIACR DLFQDTYHRY WASRQTLPLP AAS
Length:533
Mass (Da):59,232
Last modified:May 10, 2005 - v1
Checksum:i72B8FC097972E290
GO

Sequence cautioni

The sequence AAH13485 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti289R → C in AAH83324 (PubMed:15489334).Curated1
Sequence conflicti300 – 304KLLAD → SAFSS in AAH83324 (PubMed:15489334).Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC013485 mRNA. Translation: AAH13485.1. Different initiation.
BC083324 mRNA. Translation: AAH83324.1.
BC093498 mRNA. Translation: AAH93498.1.
CCDSiCCDS39847.1.
UniGeneiMm.124728.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC013485 mRNA. Translation: AAH13485.1. Different initiation.
BC083324 mRNA. Translation: AAH83324.1.
BC093498 mRNA. Translation: AAH93498.1.
CCDSiCCDS39847.1.
UniGeneiMm.124728.

3D structure databases

ProteinModelPortaliQ566J8.
SMRiQ566J8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000003860.

PTM databases

PhosphoSitePlusiQ566J8.

Proteomic databases

EPDiQ566J8.
MaxQBiQ566J8.
PaxDbiQ566J8.
PRIDEiQ566J8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1924139. Adck4.

Phylogenomic databases

eggNOGiKOG1234. Eukaryota.
COG0661. LUCA.
HOGENOMiHOG000201140.
HOVERGENiHBG061318.
InParanoidiQ566J8.
PhylomeDBiQ566J8.

Miscellaneous databases

PROiQ566J8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000003762.
CleanExiMM_ADCK4.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR004147. UbiB_dom.
[Graphical view]
PfamiPF03109. ABC1. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiCOQ8B_MOUSE
AccessioniPrimary (citable) accession number: Q566J8
Secondary accession number(s): Q5XJH7, Q91WT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: May 10, 2005
Last modified: November 30, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.