Reviewed, UniProtKB/Swiss-Prot Q56660 (SCRB2_VIBCH)

Names and origin

Protein names	Recommended name:     Probable sucrose-6-phosphate hydrolase       Short name=Sucrase     EC=3.2.1.26 Alternative name(s):     Invertase
Organism	Vibrio cholerae
Taxonomic identifier	666 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	546 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Enables the bacterium to metabolize sucrose as a sole carbon source By similarity.
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Pathway	Glycan biosynthesis; sucrose metabolism.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-fructofuranosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 546	546	Probable sucrose-6-phosphate hydrolase		PRO_0000341298
Regions
	Region	105 – 108	4	Substrate binding By similarity
	Region	167 – 168	2	Substrate binding By similarity
	Region	228 – 229	2	Substrate binding By similarity
Sites
	Active site	108	1	By similarity
	Binding site	124	1	Substrate By similarity
	Binding site	283	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q56660-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: BC6FF116B1A40CB4	FASTA	546	62,334
	10 20 30 40 50 60 MLLDTQLELA GGINNVTRIL APQGQVVLAL KHPPHAPHLP DDVSLQSVLG EWQLSVQRTA 70 80 90 100 110 120 EVSDQQLATI GKAISERQKL ETLPYQTALD CPYRPLWHIS PPQGLLNDPN GFIYHQGEYH 130 140 150 160 170 180 LFYQWHPFVC EHKDKYWVHL KSLDLVHWQW QSVALTPSDW FDSHGVFSGH AVSHQQDLWL 190 200 210 220 230 240 FYTGNTRLGV DRQRQTMQCA ARMNANGEFE KLGPVIRCLP EGVTEHIRDP KVIYTQGKWH 250 260 270 280 290 300 MLLGAQTLAH QGRLAVYHSD DLLHWHFDKL YGDELGNYGY MWECPDWFEL QGEAFFVFGP 310 320 330 340 350 360 QGIASANPHH TIEHQNRIFR ATQNAQGEIA LLQGWPLDEG FDFYAPQTAQ TTDGRRVLCG 370 380 390 400 410 420 WMGLPDETQH PSCDQGWIHQ LTALRELEWR EGKIYQHPLR ELDTLRSEPH TLLLSDTVTE 430 440 450 460 470 480 LKAKSFDVQV TLPWGCQLRL MQNAQYCVTL TLDAENRLLR LDRSATQIRQ GDTIRELKLD 490 500 510 520 530 540 SPTVELRILA DQSSLEIFIN QGEHVMTSRI FTPLDATGIS LHGASVDAKL YYMAPASAPF NLEVNV

References

[1]	Chon S.Y. Submitted (FEB-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BV6.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M74035 Genomic DNA. Translation: AAA27560.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Family and domain databases
InterPro	IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR006232. Suc6P_hydrolase. [Graphical view]
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
TIGRFAMs	TIGR01322. scrB_fam. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SCRB2_VIBCH

Accession

Primary (citable) accession number: Q56660

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)