Q56632 (VIBA_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vibriobactin-specific 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
EC=1.3.1.28

Gene names

Name:	vibA
Ordered Locus Names:	VC_0774

Organism

Vibrio cholerae

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in an early step of the biosynthesis of the catechol siderophore vibriobactin. Vibriobactin is a chelating compound involved in transporting iron from the bacterial environment into the cell cytoplasm.
Catalytic activity	2,3-dihydro-2,3-dihydroxybenzoate + NAD+ = 2,3-dihydroxybenzoate + NADH.
Pathway	Siderophore biosynthesis; vibriobactin biosynthesis.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Caution	It is uncertain whether Met-1 or Met-4 is the initiator.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	enterobactin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 262	262	Vibriobactin-specific 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase		PRO_0000054808
Regions
	Nucleotide binding	12 – 36	25	NAD By similarity
Sites
	Active site	158	1	Proton acceptor By similarity
	Binding site	145	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q56632 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 977C9CB943E800CD	FASTA	262	28,093
	10 20 30 40 50 60 MKDMSLVNSK VLLVGSARGI GFSVLEHLLQ AGAQVMAADC EWQLLLEQSE SLLGRYPDQL 70 80 90 100 110 120 TLKKLDLAEP EAVREQVNQW AEQVAGFDHL VCCAGILHVA PLHEMPMEQV SSIFTVNAFG 130 140 150 160 170 180 VLACMQGVAS SMKARQQGSM VIIGSNAANT PRMSIGAYGA SKAALHMLVK CIGMELAPYG 190 200 210 220 230 240 IRCNLVSPGS TRTAMQQQLW TEQYGEAQVI AGDAAQFRLG IPLNKIAEPA DIAQAVLFLL 250 260 SDNAGHITLH DLRVDGGATL DH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a Vibrio cholerae vibriobactin gene cluster: identification of genes required for early steps in siderophore biosynthesis." Wyckoff E.E., Stoebner J.A., Reed K.E., Payne S.M. J. Bacteriol. 179:7055-7062(1997) [PubMed: 9371453] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: El Tor Lou15.
[2]	"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed: 10952301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961 / Serotype O1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U52150 Genomic DNA. Translation: AAC45924.1. AE003852 Genomic DNA. Translation: AAF93939.1.
PIR	G82280.
RefSeq	NP_230423.1. NC_002505.1.
3D structure databases
ProteinModelPortal	Q56632.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2615317.
GenomeReviews	Gene locus VC_0774 in contig AE003852_GR.
KEGG	vch:VC0774.
PATRIC	20080659. VBIVibCho83274_0738.
TIGR	VC_0774.
Phylogenomic databases
HOGENOM	HBG750976.
OMA	NNIVESD.
PhylomeDB	Q56632.
ProtClustDB	PRK08220.
Enzyme and pathway databases
BioCyc	MetaCyc:VC0774-MONOMER. VCHO:VC0774-MONOMER.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR003560. DHB_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00216.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR01397. DHBDHDRGNASE.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VIBA_VIBCH

Accession

Primary (citable) accession number: Q56632
Secondary accession number(s): Q9JQ08

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program