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Protein

Na(+)-translocating NADH-quinone reductase subunit F

Gene

nqrF

Organism
Vibrio alginolyticus
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na+ ions from the cytoplasm to the periplasm. The first step is catalyzed by NqrF, which accepts electrons from NADH and reduces ubiquinone-1 to ubisemiquinone by a one-electron transfer pathway.UniRule annotation2 Publications

Catalytic activityi

NADH + ubiquinone + n Na+(In) = NAD+ + ubiquinol + n Na+(Out).UniRule annotation1 Publication

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster.UniRule annotation
  • FADUniRule annotation1 Publication

Enzyme regulationi

Activated by Na+ or K+. Inhibited by silver.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi69 – 691Iron-sulfur (2Fe-2S)UniRule annotation
Metal bindingi75 – 751Iron-sulfur (2Fe-2S)UniRule annotation
Metal bindingi78 – 781Iron-sulfur (2Fe-2S)UniRule annotation
Metal bindingi110 – 1101Iron-sulfur (2Fe-2S)UniRule annotation

GO - Molecular functioni

GO - Biological processi

  • oxidation-reduction process Source: UniProtKB
  • sodium ion transport Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, NAD, Sodium, Ubiquinone

Enzyme and pathway databases

BRENDAi1.6.5.8. 6624.

Protein family/group databases

TCDBi3.D.5.1.1. the na(+)-translocating nadh:quinone dehydrogenase (na-ndh or nqr) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Na(+)-translocating NADH-quinone reductase subunit F1 PublicationUniRule annotation (EC:1.6.5.8UniRule annotation1 Publication)
Short name:
Na(+)-NQR subunit FUniRule annotation
Short name:
Na(+)-translocating NQR subunit FUniRule annotation
Alternative name(s):
NQR complex subunit FUniRule annotation
NQR-1 subunit FUniRule annotation
Gene namesi
Name:nqrFUniRule annotation
Synonyms:nqr61 Publication
OrganismiVibrio alginolyticus
Taxonomic identifieri663 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Subcellular locationi

  • Cell inner membrane UniRule annotationCurated; Single-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei3 – 2321HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 407407Na(+)-translocating NADH-quinone reductase subunit FPRO_0000074504Add
BLAST

Post-translational modificationi

The N-terminus is blocked.1 Publication

Interactioni

Subunit structurei

Composed of six subunits; NqrA, NqrB, NqrC, NqrD, NqrE and NqrF.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi674977.VMC_17380.

Structurei

3D structure databases

ProteinModelPortaliQ56584.
SMRiQ56584. Positions 128-406.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 126952Fe-2S ferredoxin-typeUniRule annotationAdd
BLAST
Domaini129 – 269141FAD-binding FR-typeUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni272 – 389118CatalyticAdd
BLAST

Sequence similaritiesi

Belongs to the NqrF family.UniRule annotationCurated
Contains 1 2Fe-2S ferredoxin-type domain.UniRule annotation
Contains 1 FAD-binding FR-type domain.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D26. Bacteria.
COG2871. LUCA.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
HAMAPiMF_00430. NqrF.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR010205. NqrF.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00970. FAD_binding_6. 1 hit.
PF00111. Fer2. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000044. Cis_Diol_DH_RD. 1 hit.
PRINTSiPR00371. FPNCR.
SUPFAMiSSF54292. SSF54292. 1 hit.
SSF63380. SSF63380. 1 hit.
TIGRFAMsiTIGR01941. nqrF. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q56584-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIILGVVMF TLIVLALVLV ILFAKSKLVP TGDITISVND DPSLAIVTQP
60 70 80 90 100
GGKLLSALAG AGVFVSSACG GGGSCGQCRV KVKSGGGDIL PTELDHITKG
110 120 130 140 150
EAREGERLAC QVAMKTDMDI ELPEEIFGVK KWECTVISND NKATFIKELK
160 170 180 190 200
LQIPDGESVP FRAGGYIQIE APAHHVKYAD YDIPEEYRED WEKFNLFRYE
210 220 230 240 250
SKVNEETIRA YSMANYPEEH GIIMLNVRIA TPPPNNPDVP PGIMSSYIWS
260 270 280 290 300
LKEGDKCTIS GPFGEFFAKD TDAEMVFVGG GAGMAPMRSH IFDQLKRLHS
310 320 330 340 350
KRKMSFWYGA RSKREMFYVE DFDMLQAEND NFVWHCALSD PLPEDNWDGY
360 370 380 390 400
TGFIHNVLYE NYLRDHEAPE DCEYYMCGPP MMNAAVIGML KDLGVEDENI

LLDDFGG
Length:407
Mass (Da):45,275
Last modified:November 1, 1996 - v1
Checksum:iA878E89DFCA87346
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008030 Genomic DNA. Translation: BAA22915.1.
PIRiS65531.
RefSeqiWP_005380271.1. NZ_LRIG01000001.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008030 Genomic DNA. Translation: BAA22915.1.
PIRiS65531.
RefSeqiWP_005380271.1. NZ_LRIG01000001.1.

3D structure databases

ProteinModelPortaliQ56584.
SMRiQ56584. Positions 128-406.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi674977.VMC_17380.

Protein family/group databases

TCDBi3.D.5.1.1. the na(+)-translocating nadh:quinone dehydrogenase (na-ndh or nqr) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105D26. Bacteria.
COG2871. LUCA.

Enzyme and pathway databases

BRENDAi1.6.5.8. 6624.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
HAMAPiMF_00430. NqrF.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR010205. NqrF.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00970. FAD_binding_6. 1 hit.
PF00111. Fer2. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000044. Cis_Diol_DH_RD. 1 hit.
PRINTSiPR00371. FPNCR.
SUPFAMiSSF54292. SSF54292. 1 hit.
SSF63380. SSF63380. 1 hit.
TIGRFAMsiTIGR01941. nqrF. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequencing and the alignment of structural genes in the nqr operon encoding the Na(+)-translocating NADH-quinone reductase from Vibrio alginolyticus."
    Hayashi M., Hirai K., Unemoto T.
    FEBS Lett. 363:75-77(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Expression and analysis of the gene for the catalytic beta subunit of the sodium-translocating NADH-ubiquinone oxidoreductase of Vibrio alginolyticus."
    Tan K., Beattie P., Leach D.R.F., Rich P.R., Coulson A.F.W., Ward F.B.
    Biochem. Soc. Trans. 24:12S-12S(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Identification of six subunits constituting Na+-translocating NADH-quinone reductase from the marine Vibrio alginolyticus."
    Nakayama Y., Hayashi M., Unemoto T.
    FEBS Lett. 422:240-242(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-10, CATALYTIC ACTIVITY, SUBUNIT.
  4. "The Na+-translocating NADH:ubiquinone oxidoreductase from Vibrio alginolyticus -- redox states of the FAD prosthetic group and mechanism of Ag+ inhibition."
    Steuber J., Krebs W., Dimroth P.
    Eur. J. Biochem. 249:770-776(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: COFACTOR, ENZYME REGULATION.
    Strain: ATCC 17749 / DSM 2171 / NBRC 15630 / NCIMB 1903 / XII-53.
  5. "Modifications by Na+ and K+, and the site of Ag+ inhibition in the Na+-translocating NADH-quinone reductase from a marine Vibrio alginolyticus."
    Unemoto T., Ogura T., Hayashi M.
    Biochim. Biophys. Acta 1183:201-205(1993)
    Cited for: INHIBITION OF ENZYMATIC ACTIVITY.
  6. "Inhibitor studies of a new antibiotic, korormicin, 2-n-heptyl-4-hydroxyquinoline N-oxide and Ag+ toward the Na+-translocating NADH-quinone reductase from the marine Vibrio alginolyticus."
    Nakayama Y., Hayashi M., Yoshikawa K., Mochida K., Unemoto T.
    Biol. Pharm. Bull. 22:1064-1067(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INHIBITION OF ENZYMATIC ACTIVITY.
  7. "Recent progress in the Na(+)-translocating NADH-quinone reductase from the marine Vibrio alginolyticus."
    Hayashi M., Nakayama Y., Unemoto T.
    Biochim. Biophys. Acta 1505:37-44(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  8. "Na(+) translocation by bacterial NADH:quinone oxidoreductases: an extension to the complex-I family of primary redox pumps."
    Steuber J.
    Biochim. Biophys. Acta 1505:45-56(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiNQRF_VIBAL
AccessioniPrimary (citable) accession number: Q56584
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.