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Q56366

- DPOL_THES9

UniProt

Q56366 - DPOL_THES9

Protein

DNA polymerase

Gene

pol

Organism
Thermococcus sp. (strain 9oN-7)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
    3. nucleotide binding Source: InterPro

    Keywords - Molecular functioni

    DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymerase (EC:2.7.7.7)
    Gene namesi
    Name:pol
    Synonyms:polA
    OrganismiThermococcus sp. (strain 9oN-7)
    Taxonomic identifieri103799 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 775775DNA polymerasePRO_0000046489Add
    BLAST

    Structurei

    Secondary structure

    1
    775
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 109
    Beta strandi13 – 2210
    Beta strandi25 – 317
    Beta strandi37 – 437
    Helixi45 – 473
    Helixi48 – 514
    Beta strandi55 – 584
    Beta strandi61 – 644
    Beta strandi67 – 759
    Beta strandi78 – 869
    Helixi92 – 10110
    Beta strandi106 – 1116
    Helixi116 – 1238
    Beta strandi137 – 1448
    Beta strandi157 – 1637
    Beta strandi168 – 1747
    Beta strandi179 – 1835
    Helixi187 – 20115
    Beta strandi204 – 2107
    Turni211 – 2144
    Helixi215 – 22511
    Beta strandi240 – 2445
    Beta strandi247 – 2515
    Beta strandi255 – 2595
    Helixi260 – 2678
    Helixi275 – 2839
    Helixi292 – 2998
    Helixi305 – 33733
    Helixi341 – 3444
    Helixi349 – 36315
    Helixi374 – 3796
    Turni380 – 3823
    Beta strandi397 – 4048
    Helixi408 – 4158
    Turni420 – 4223
    Beta strandi431 – 4333
    Turni435 – 4373
    Beta strandi440 – 4423
    Helixi448 – 46821
    Helixi473 – 49018
    Helixi493 – 4986
    Helixi507 – 53226
    Beta strandi535 – 5406
    Beta strandi543 – 5475
    Helixi553 – 56715
    Helixi568 – 5703
    Beta strandi578 – 59013
    Beta strandi593 – 5975
    Beta strandi604 – 6074
    Helixi609 – 6113
    Helixi617 – 63317
    Helixi636 – 64510
    Helixi657 – 6604
    Beta strandi662 – 6654
    Helixi670 – 6723
    Helixi677 – 68711
    Turni688 – 6903
    Beta strandi701 – 7033
    Helixi710 – 7123
    Beta strandi713 – 7164
    Helixi717 – 7193
    Turni722 – 7243
    Helixi731 – 7344
    Helixi737 – 7404
    Helixi742 – 7465
    Turni747 – 7493
    Helixi753 – 7553

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1QHTX-ray2.10A1-775[»]
    4K8XX-ray2.28A1-775[»]
    ProteinModelPortaliQ56366.
    SMRiQ56366. Positions 1-750.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ56366.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DNA polymerase type-B family.Curated

    Family and domain databases

    Gene3Di3.30.420.10. 1 hit.
    3.90.1600.10. 2 hits.
    InterProiIPR006172. DNA-dir_DNA_pol_B.
    IPR017964. DNA-dir_DNA_pol_B_CS.
    IPR006133. DNA-dir_DNA_pol_B_exonuc.
    IPR006134. DNA-dir_DNA_pol_B_multi_dom.
    IPR023211. DNA_pol_palm_dom.
    IPR012337. RNaseH-like_dom.
    [Graphical view]
    PfamiPF00136. DNA_pol_B. 1 hit.
    PF03104. DNA_pol_B_exo1. 2 hits.
    [Graphical view]
    PRINTSiPR00106. DNAPOLB.
    SMARTiSM00486. POLBc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53098. SSF53098. 1 hit.
    PROSITEiPS00116. DNA_POLYMERASE_B. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q56366-1 [UniParc]FASTAAdd to Basket

    « Hide

    MILDTDYITE NGKPVIRVFK KENGEFKIEY DRTFEPYFYA LLKDDSAIED    50
    VKKVTAKRHG TVVKVKRAEK VQKKFLGRPI EVWKLYFNHP QDVPAIRDRI 100
    RAHPAVVDIY EYDIPFAKRY LIDKGLIPME GDEELTMLAF DIETLYHEGE 150
    EFGTGPILMI SYADGSEARV ITWKKIDLPY VDVVSTEKEM IKRFLRVVRE 200
    KDPDVLITYN GDNFDFAYLK KRCEELGIKF TLGRDGSEPK IQRMGDRFAV 250
    EVKGRIHFDL YPVIRRTINL PTYTLEAVYE AVFGKPKEKV YAEEIAQAWE 300
    SGEGLERVAR YSMEDAKVTY ELGREFFPME AQLSRLIGQS LWDVSRSSTG 350
    NLVEWFLLRK AYKRNELAPN KPDERELARR RGGYAGGYVK EPERGLWDNI 400
    VYLDFRSLYP SIIITHNVSP DTLNREGCKE YDVAPEVGHK FCKDFPGFIP 450
    SLLGDLLEER QKIKRKMKAT VDPLEKKLLD YRQRAIKILA NSFYGYYGYA 500
    KARWYCKECA ESVTAWGREY IEMVIRELEE KFGFKVLYAD TDGLHATIPG 550
    ADAETVKKKA KEFLKYINPK LPGLLELEYE GFYVRGFFVT KKKYAVIDEE 600
    GKITTRGLEI VRRDWSEIAK ETQARVLEAI LKHGDVEEAV RIVKEVTEKL 650
    SKYEVPPEKL VIHEQITRDL RDYKATGPHV AVAKRLAARG VKIRPGTVIS 700
    YIVLKGSGRI GDRAIPADEF DPTKHRYDAE YYIENQVLPA VERILKAFGY 750
    RKEDLRYQKT KQVGLGAWLK VKGKK 775
    Length:775
    Mass (Da):89,781
    Last modified:November 1, 1996 - v1
    Checksum:i71525D63C0C9CD9D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U47108 Genomic DNA. Translation: AAA88769.1.
    PIRiS67920.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U47108 Genomic DNA. Translation: AAA88769.1 .
    PIRi S67920.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1QHT X-ray 2.10 A 1-775 [» ]
    4K8X X-ray 2.28 A 1-775 [» ]
    ProteinModelPortali Q56366.
    SMRi Q56366. Positions 1-750.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q56366.

    Family and domain databases

    Gene3Di 3.30.420.10. 1 hit.
    3.90.1600.10. 2 hits.
    InterProi IPR006172. DNA-dir_DNA_pol_B.
    IPR017964. DNA-dir_DNA_pol_B_CS.
    IPR006133. DNA-dir_DNA_pol_B_exonuc.
    IPR006134. DNA-dir_DNA_pol_B_multi_dom.
    IPR023211. DNA_pol_palm_dom.
    IPR012337. RNaseH-like_dom.
    [Graphical view ]
    Pfami PF00136. DNA_pol_B. 1 hit.
    PF03104. DNA_pol_B_exo1. 2 hits.
    [Graphical view ]
    PRINTSi PR00106. DNAPOLB.
    SMARTi SM00486. POLBc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53098. SSF53098. 1 hit.
    PROSITEi PS00116. DNA_POLYMERASE_B. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of thermostable DNA polymerases from hyperthermophilic marine Archaea with emphasis on Thermococcus sp. 9oN-7 and mutations affecting 3'-5' exonuclease activity."
      Southworth M.W., Kong H., Kucera R.B., Ware J., Jannasch H.W., Perler F.B.
      Proc. Natl. Acad. Sci. U.S.A. 93:5281-5285(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiDPOL_THES9
    AccessioniPrimary (citable) accession number: Q56366
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3