Reviewed,
UniProtKB/Swiss-Prot Q56350 (NAPA_PARPN)
Last modified
June 16, 2009.
Version 66.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Periplasmic nitrate reductase EC=1.7.99.4 | ||
| Gene names |
| ||
| Organism | Paracoccus pantotrophus (Thiosphaera pantotropha) | ||
| Taxonomic identifier | 82367 [NCBI] | ||
| Taxonomic lineage | Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Paracoccus |
Protein attributes
| Sequence length | 831 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism. HAMAP MF_01630 |
| Catalytic activity | Nitrite + acceptor = nitrate + reduced acceptor. HAMAP MF_01630 |
| Cofactor | Binds 1 4Fe-4S cluster By similarity. Binds 1 molybdenum ion per subunit. HAMAP MF_01630 Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit. HAMAP MF_01630 |
| Subunit structure | Interacts with napB By similarity. |
| Subcellular location | |
| Post-translational modification | Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP MF_01630 |
| Sequence similarities | Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 31 | 31 | Tat-type signal Potential | ||||||
| Chain | 32 – 831 | 800 | Periplasmic nitrate reductase HAMAP MF_01630 | PRO_0000019172 | |||||
Sites | |||||||||
| Metal binding | 48 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 51 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 55 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
| Metal binding | 83 | 1 | Iron-sulfur (4Fe-4S) By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 128 | 1 | T → D AA sequence Ref.1 | ||||||
Sequences
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References
| [1] | "The napEDABC gene cluster encoding the periplasmic nitrate reductase system of Thiosphaera pantotropha." Berks B.C., Richardson D.J., Reilly A., Willis A.C., Ferguson S.J. Biochem. J. 309:983-992(1995) [PubMed: 7639719] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: ATCC 35512 / DSM 2944 / LMD 82.5 / NCCB 82005. |
| [2] | "Purification and characterization of the periplasmic nitrate reductase from Thiosphaera pantotropha." Berks B.C., Richardson D.J., Robinson C., Reilly A., Aplin R.T., Ferguson S.J. Eur. J. Biochem. 220:117-124(1994) [PubMed: 8119278] [Abstract] Cited for: CHARACTERIZATION. Strain: M-6. |
| [3] | "Characterization of the paramagnetic iron-containing redox centres of Thiosphaera pantotropha periplasmic nitrate reductase." Breton J., Berks B.C., Reilly A., Thomson A.J., Ferguson S.J., Richardson D.J. FEBS Lett. 345:76-80(1994) [PubMed: 8194605] [Abstract] Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF 642-655 AND 699-715. Strain: M-6. |
Cross-references
Sequence databases | |
|---|---|
| Z36773 Genomic DNA. Translation: CAA85346.1. | |
| PIR | S50163. |
3D structure databases | |
| HSSP | HSSP built from PDB template 2NAP based on UniProtKB P81186. |
| ModBase | Search... |
Enzyme and pathway databases | |
| BRENDA | 1.7.99.4. 258307. |
Family and domain databases | |
| HAMAP | MF_01630. [Tree] |
| InterPro | IPR009010. Asp_de-COase-like_fold. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006657. MPT_dinuc_bd. IPR010051. NO3_reductase_lsu_periplasm. IPR006311. Tat. IPR017909. Twin_arg_translocation_Tat. [Graphical view] |
| Gene3D | G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit. |
| Pfam | PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit. |
| PROSITE | PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | NAPA_PARPN | ||||||||
| Accession | Primary (citable) accession number: Q56350 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||

Clusters with


