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Q56350 (NAPA_PARPN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
OrganismParacoccus pantotrophus (Thiosphaera pantotropha)
Taxonomic identifier82367 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeParacoccus

Protein attributes

Sequence length831 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131Tat-type signal Potential
Chain32 – 831800Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_0000019172

Regions

Domain41 – 97574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding481Iron-sulfur (4Fe-4S) By similarity
Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding551Iron-sulfur (4Fe-4S) By similarity
Metal binding831Iron-sulfur (4Fe-4S) By similarity

Experimental info

Sequence conflict1281T → D AA sequence Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q56350 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: ED78963BD2DA2144

FASTA83192,618
        10         20         30         40         50         60 
MTISRRDLLK AQAAGIAAMA ANIPLSSQAP AVPGGVESLQ ITWSKAPCRF CGTGCGVMVG 

        70         80         90        100        110        120 
VKEGRVVATH GDLLAEVNRG LNCVKGYFLS KIMYGADRLT QPLLRKKDGV YAKDGEFTPV 

       130        140        150        160        170        180 
SWEEAFDTMA AQAKRVLRDK GPTALGMFGS GQWTIFEGYA ATKLMRAGFR SNNLDPNARH 

       190        200        210        220        230        240 
CMASAAYAFM RTFGMDEPMG CYDDFEAADA FVLWGSNMAE MHPILWTRVA DRRLGHPHVK 

       250        260        270        280        290        300 
VAVLSTFTHR SSDLADIPIV FKPGTDLAIL NYIANHIIQT GRVNRDFVDR HTTFVAGATG 

       310        320        330        340        350        360 
IGYGLRDDDP REMAARTAED PAATTPSTFE EFAELVSEYT LDKVSELSGV EPAFLEQLAE 

       370        380        390        400        410        420 
LYADPDRKVM SLWTMGFNQH VRGVWANQMV YNLHLLTGKI SEPGNSPFSL TGQASACGTA 

       430        440        450        460        470        480 
RQVGTFRHRL PSDMTVTNPE RRQDAEEIWR IPHGVIPEQP GLHAVAQDRA LHDGTLNFYW 

       490        500        510        520        530        540 
IQVNNNLQAS PNNDGEAWPG YRNPDNFIVV SDAYPTVTAL AADLILPAAM WVEKEGAYGN 

       550        560        570        580        590        600 
AERRTHVWHQ LVEAPGEARS DLWQMMEFST RFTTDEVWPE EILAANPNYR GQSLFDVLFR 

       610        620        630        640        650        660 
NGSVDRFDLS ELNPVTPTAE SNAFGFYVQK GLFEEYAPFG RGHGHDLAPY DTYHEVRGLR 

       670        680        690        700        710        720 
WPVVDGKETL WRYREGLDPY VEPGAGVQFY GNPDGKARII AVPYEPPAEP PDEEYNIWLV 

       730        740        750        760        770        780 
TGRVLEHWHS GSMTMRVPEL YRAFPGARCF MNPEDARDMG FNQGAEVRIV SRRGEIRSRV 

       790        800        810        820        830 
ETRGRNRMPR GVVFVPWFDA SQLINKVTLD ATDPISKQTD FKKCAVKILP V 

« Hide

References

[1]"The napEDABC gene cluster encoding the periplasmic nitrate reductase system of Thiosphaera pantotropha."
Berks B.C., Richardson D.J., Reilly A., Willis A.C., Ferguson S.J.
Biochem. J. 309:983-992(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
Strain: ATCC 35512 / DSM 2944 / LMD 82.5 / NCCB 82005.
[2]"Purification and characterization of the periplasmic nitrate reductase from Thiosphaera pantotropha."
Berks B.C., Richardson D.J., Robinson C., Reilly A., Aplin R.T., Ferguson S.J.
Eur. J. Biochem. 220:117-124(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
Strain: M-6.
[3]"Characterization of the paramagnetic iron-containing redox centres of Thiosphaera pantotropha periplasmic nitrate reductase."
Breton J., Berks B.C., Reilly A., Thomson A.J., Ferguson S.J., Richardson D.J.
FEBS Lett. 345:76-80(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF 642-655 AND 699-715.
Strain: M-6.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z36773 Genomic DNA. Translation: CAA85346.1.
PIRS50163.

3D structure databases

ProteinModelPortalQ56350.
SMRQ56350. Positions 41-828.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_PARPN
AccessionPrimary (citable) accession number: Q56350
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families