Q56320 (TRPF_THEMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-(5'-phosphoribosyl)anthranilate isomerase
Short name=PRAI
EC=5.3.1.24

Gene names

Name:	trpF
Ordered Locus Names:	TM_0139

Organism

Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [Reference proteome] [HAMAP]

Taxonomic identifier

243274 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Thermotogae › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga › Thermotoga maritima

Protein attributes

Sequence length	205 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate. HAMAP-Rule MF_00135
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5. HAMAP-Rule MF_00135
Subunit structure	Homodimer.
Sequence similarities	Belongs to the TrpF family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Molecular function	Isomerase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tryptophan biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	phosphoribosylanthranilate isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 205

205

N-(5'-phosphoribosyl)anthranilate isomerase HAMAP-Rule MF_00135

PRO_0000154390

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

205

Helix Strand Turn

Details...

Beta strand

3 – 6

4

Helix

12 – 21

10

Beta strand

24 – 29

6

Helix

40 – 49

10

Beta strand

52 – 62

11

Helix

65 – 75

11

Beta strand

78 – 82

5

Helix

88 – 95

8

Beta strand

98 – 108

11

Helix

109 – 115

7

Helix

116 – 118

3

Beta strand

123 – 128

6

Beta strand

130 – 134

5

Helix

141 – 143

3

Helix

145 – 150

6

Beta strand

154 – 159

6

Turn

162 – 164

3

Helix

165 – 172

8

Beta strand

175 – 180

6

Helix

181 – 183

3

Beta strand

184 – 186

3

Helix

192 – 203

12

Sequences

	Sequence		Length	Mass (Da)
	Q56320 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 9E1D76948C7EAE5E	FASTA	205	23,041
	10 20 30 40 50 60 MVRVKICGIT NLEDALFSVE SGADAVGFVF YPKSKRYISP EDARRISVEL PPFVFRVGVF 70 80 90 100 110 120 VNEEPEKILD VASYVQLNAV QLHGEEPIEL CRKIAERILV IKAVGVSNER DMERALNYRE 130 140 150 160 170 180 FPILLDTKTP EYGGSGKTFD WSLILPYRDR FRYLVLSGGL NPENVRSAID VVRPFAVDVS 190 200 SGVEAFPGKK DHDSIKMFIK NAKGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"(Beta alpha)8-barrel proteins of tryptophan biosynthesis in the hyperthermophile Thermotoga maritima." Sterner R., Dahm A., Darimont B., Ivens A., Liebl W., Kirschner K. EMBO J. 14:4395-4402(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[3]	"Crystal structure at 2.0-A resolution of phosphoribosyl anthranilate isomerase from the hyperthermophile Thermotoga maritima: possible determinants of protein stability." Hennig M., Sterner R., Kirschner K., Jansonius J.N. Biochemistry 36:6009-6016(1997) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X92729 Genomic DNA. Translation: CAA63390.1.
AE000512 Genomic DNA. Translation: AAD35232.1.

PIR

S59048.

RefSeq

NP_227954.1. NC_000853.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DL3	X-ray	2.70	A/B	1-205	[»]
1LBM	X-ray	2.80	A	1-205	[»]
1NSJ	X-ray	2.00	A	1-205	[»]

ProteinModelPortal

Q56320.

SMR

Q56320. Positions 1-205.

ModBase

Search...

Protein-protein interaction databases

STRING

243274.TM0139.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAD35232; AAD35232; TM_0139.

GeneID

896969.

KEGG

tma:TM0139.

PATRIC

23935120. VBITheMar51294_0138.

Phylogenomic databases

eggNOG

COG0135.

KO

K01817.

OMA

FVNASRC.

ProtClustDB

PRK01222.

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-301.

UniPathway

UPA00035; UER00042.

Family and domain databases

Gene3D

3.20.20.70. 1 hit.

HAMAP

MF_00135. PRAI.

InterPro

IPR013785. Aldolase_TIM.
IPR001240. PRAI.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]

Pfam

PF00697. PRAI. 1 hit.
[Graphical view]

SUPFAM

SSF51366. RibP_bind_barrel. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q56320.

Entry information

Entry name

TRPF_THEMA

Accession

Primary (citable) accession number: Q56320

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program