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Protein

Holliday junction ATP-dependent DNA helicase RuvB

Gene

ruvB

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi58 – 658ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Holliday junction ATP-dependent DNA helicase RuvBUniRule annotation (EC:3.6.4.12UniRule annotation)
Gene namesi
Name:ruvBUniRule annotation
Ordered Locus Names:TM_1730
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 334334Holliday junction ATP-dependent DNA helicase RuvBPRO_0000165617Add
BLAST

Interactioni

Subunit structurei

Forms a complex with RuvA.

Protein-protein interaction databases

STRINGi243274.TM1730.

Structurei

Secondary structure

1
334
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi24 – 263Combined sources
Helixi31 – 4717Combined sources
Beta strandi54 – 596Combined sources
Helixi64 – 7512Combined sources
Beta strandi79 – 835Combined sources
Turni84 – 863Combined sources
Helixi90 – 9910Combined sources
Beta strandi105 – 1095Combined sources
Helixi111 – 1133Combined sources
Helixi116 – 12712Combined sources
Beta strandi152 – 1587Combined sources
Helixi160 – 1623Combined sources
Helixi165 – 1684Combined sources
Beta strandi172 – 1765Combined sources
Helixi182 – 19514Combined sources
Helixi202 – 2109Combined sources
Helixi216 – 23318Combined sources
Beta strandi236 – 2383Combined sources
Helixi240 – 25011Combined sources
Helixi259 – 27113Combined sources
Helixi279 – 2868Combined sources
Helixi290 – 2967Combined sources
Helixi298 – 3036Combined sources
Beta strandi306 – 3105Combined sources
Beta strandi313 – 3164Combined sources
Helixi318 – 3236Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IN4X-ray1.60A1-334[»]
1IN5X-ray2.00A1-334[»]
1IN6X-ray1.80A1-334[»]
1IN7X-ray1.90A1-334[»]
1IN8X-ray1.90A1-334[»]
1J7KX-ray1.80A1-334[»]
ProteinModelPortaliQ56313.
SMRiQ56313. Positions 17-329.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ56313.

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CKJ. Bacteria.
COG2255. LUCA.
InParanoidiQ56313.
KOiK03551.
OMAiYEPYLMQ.
OrthoDBiEOG6SR93S.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00016. DNA_helic_RuvB.
InterProiIPR003593. AAA+_ATPase.
IPR004605. DNA_helicase_Holl-junc_RuvB.
IPR008823. DNA_helicase_Holl-junc_RuvB_C.
IPR027417. P-loop_NTPase.
IPR008824. RuvB_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF05491. RuvB_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]
ProDomiPD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00635. ruvB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q56313-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEFLTPERT VYDSGVQFLR PKSLDEFIGQ ENVKKKLSLA LEAAKMRGEV
60 70 80 90 100
LDHVLLAGPP GLGKTTLAHI IASELQTNIH VTSGPVLVKQ GDMAAILTSL
110 120 130 140 150
ERGDVLFIDE IHRLNKAVEE LLYSAIEDFQ IDIMIGKGPS AKSIRIDIQP
160 170 180 190 200
FTLVGATTRS GLLSSPLRSR FGIILELDFY TVKELKEIIK RAASLMDVEI
210 220 230 240 250
EDAAAEMIAK RSRGTPRIAI RLTKRVRDML TVVKADRINT DIVLKTMEVL
260 270 280 290 300
NIDDEGLDEF DRKILKTIIE IYRGGPVGLN ALAASLGVEA DTLSEVYEPY
310 320 330
LLQAGFLART PRGRIVTEKA YKHLKYEVPE NRLF
Length:334
Mass (Da):37,156
Last modified:November 1, 1997 - v1
Checksum:i6F4BBFBFA7B9C7A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38840 Genomic DNA. Translation: AAB03727.1.
AE000512 Genomic DNA. Translation: AAD36795.1.
PIRiA72217.
RefSeqiNP_229528.1. NC_000853.1.
WP_004082245.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD36795; AAD36795; TM_1730.
GeneIDi897204.
KEGGitma:TM1730.
PATRICi23938436. VBITheMar51294_1748.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38840 Genomic DNA. Translation: AAB03727.1.
AE000512 Genomic DNA. Translation: AAD36795.1.
PIRiA72217.
RefSeqiNP_229528.1. NC_000853.1.
WP_004082245.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IN4X-ray1.60A1-334[»]
1IN5X-ray2.00A1-334[»]
1IN6X-ray1.80A1-334[»]
1IN7X-ray1.90A1-334[»]
1IN8X-ray1.90A1-334[»]
1J7KX-ray1.80A1-334[»]
ProteinModelPortaliQ56313.
SMRiQ56313. Positions 17-329.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM1730.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36795; AAD36795; TM_1730.
GeneIDi897204.
KEGGitma:TM1730.
PATRICi23938436. VBITheMar51294_1748.

Phylogenomic databases

eggNOGiENOG4105CKJ. Bacteria.
COG2255. LUCA.
InParanoidiQ56313.
KOiK03551.
OMAiYEPYLMQ.
OrthoDBiEOG6SR93S.

Miscellaneous databases

EvolutionaryTraceiQ56313.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00016. DNA_helic_RuvB.
InterProiIPR003593. AAA+_ATPase.
IPR004605. DNA_helicase_Holl-junc_RuvB.
IPR008823. DNA_helicase_Holl-junc_RuvB_C.
IPR027417. P-loop_NTPase.
IPR008824. RuvB_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF05491. RuvB_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]
ProDomiPD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00635. ruvB. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing, and expression of ruvB and characterization of RuvB proteins from two distantly related thermophilic eubacteria."
    Tong J., Wetmur J.G.
    J. Bacteriol. 178:2695-2700(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Entry informationi

Entry nameiRUVB_THEMA
AccessioniPrimary (citable) accession number: Q56313
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 13, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.