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Protein

Holliday junction ATP-dependent DNA helicase RuvB

Gene

ruvB

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi58 – 65ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Holliday junction ATP-dependent DNA helicase RuvBUniRule annotation (EC:3.6.4.12UniRule annotation)
Gene namesi
Name:ruvBUniRule annotation
Ordered Locus Names:TM_1730
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001656171 – 334Holliday junction ATP-dependent DNA helicase RuvBAdd BLAST334

Interactioni

Subunit structurei

Forms a complex with RuvA.

Protein-protein interaction databases

STRINGi243274.TM1730.

Structurei

Secondary structure

1334
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi24 – 26Combined sources3
Helixi31 – 47Combined sources17
Beta strandi54 – 59Combined sources6
Helixi64 – 75Combined sources12
Beta strandi79 – 83Combined sources5
Turni84 – 86Combined sources3
Helixi90 – 99Combined sources10
Beta strandi105 – 109Combined sources5
Helixi111 – 113Combined sources3
Helixi116 – 127Combined sources12
Beta strandi152 – 158Combined sources7
Helixi160 – 162Combined sources3
Helixi165 – 168Combined sources4
Beta strandi172 – 176Combined sources5
Helixi182 – 195Combined sources14
Helixi202 – 210Combined sources9
Helixi216 – 233Combined sources18
Beta strandi236 – 238Combined sources3
Helixi240 – 250Combined sources11
Helixi259 – 271Combined sources13
Helixi279 – 286Combined sources8
Helixi290 – 296Combined sources7
Helixi298 – 303Combined sources6
Beta strandi306 – 310Combined sources5
Beta strandi313 – 316Combined sources4
Helixi318 – 323Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IN4X-ray1.60A1-334[»]
1IN5X-ray2.00A1-334[»]
1IN6X-ray1.80A1-334[»]
1IN7X-ray1.90A1-334[»]
1IN8X-ray1.90A1-334[»]
1J7KX-ray1.80A1-334[»]
ProteinModelPortaliQ56313.
SMRiQ56313.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ56313.

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CKJ. Bacteria.
COG2255. LUCA.
InParanoidiQ56313.
KOiK03551.
OMAiYEPYLMQ.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00016. DNA_helic_RuvB. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR004605. DNA_helicase_Holl-junc_RuvB.
IPR008823. DNA_helicase_Holl-junc_RuvB_C.
IPR027417. P-loop_NTPase.
IPR008824. RuvB_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF05491. RuvB_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]
ProDomiPD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00635. ruvB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q56313-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEFLTPERT VYDSGVQFLR PKSLDEFIGQ ENVKKKLSLA LEAAKMRGEV
60 70 80 90 100
LDHVLLAGPP GLGKTTLAHI IASELQTNIH VTSGPVLVKQ GDMAAILTSL
110 120 130 140 150
ERGDVLFIDE IHRLNKAVEE LLYSAIEDFQ IDIMIGKGPS AKSIRIDIQP
160 170 180 190 200
FTLVGATTRS GLLSSPLRSR FGIILELDFY TVKELKEIIK RAASLMDVEI
210 220 230 240 250
EDAAAEMIAK RSRGTPRIAI RLTKRVRDML TVVKADRINT DIVLKTMEVL
260 270 280 290 300
NIDDEGLDEF DRKILKTIIE IYRGGPVGLN ALAASLGVEA DTLSEVYEPY
310 320 330
LLQAGFLART PRGRIVTEKA YKHLKYEVPE NRLF
Length:334
Mass (Da):37,156
Last modified:November 1, 1997 - v1
Checksum:i6F4BBFBFA7B9C7A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38840 Genomic DNA. Translation: AAB03727.1.
AE000512 Genomic DNA. Translation: AAD36795.1.
PIRiA72217.
RefSeqiNP_229528.1. NC_000853.1.
WP_004082245.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD36795; AAD36795; TM_1730.
GeneIDi897204.
KEGGitma:TM1730.
PATRICi23938436. VBITheMar51294_1748.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38840 Genomic DNA. Translation: AAB03727.1.
AE000512 Genomic DNA. Translation: AAD36795.1.
PIRiA72217.
RefSeqiNP_229528.1. NC_000853.1.
WP_004082245.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IN4X-ray1.60A1-334[»]
1IN5X-ray2.00A1-334[»]
1IN6X-ray1.80A1-334[»]
1IN7X-ray1.90A1-334[»]
1IN8X-ray1.90A1-334[»]
1J7KX-ray1.80A1-334[»]
ProteinModelPortaliQ56313.
SMRiQ56313.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM1730.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36795; AAD36795; TM_1730.
GeneIDi897204.
KEGGitma:TM1730.
PATRICi23938436. VBITheMar51294_1748.

Phylogenomic databases

eggNOGiENOG4105CKJ. Bacteria.
COG2255. LUCA.
InParanoidiQ56313.
KOiK03551.
OMAiYEPYLMQ.

Miscellaneous databases

EvolutionaryTraceiQ56313.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00016. DNA_helic_RuvB. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR004605. DNA_helicase_Holl-junc_RuvB.
IPR008823. DNA_helicase_Holl-junc_RuvB_C.
IPR027417. P-loop_NTPase.
IPR008824. RuvB_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF05491. RuvB_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]
ProDomiPD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00635. ruvB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRUVB_THEMA
AccessioniPrimary (citable) accession number: Q56313
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.