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Q56310

- CHEA_THEMA

UniProt

Q56310 - CHEA_THEMA

Protein

Chemotaxis protein CheA

Gene

cheA

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. CheA is autophosphorylated; it can transfer its phosphate group to either CheB or CheY By similarity.By similarity

    Catalytic activityi

    ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. phosphorelay sensor kinase activity Source: InterPro

    GO - Biological processi

    1. cellular component movement Source: InterPro
    2. chemotaxis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Chemotaxis, Two-component regulatory system

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.13.3. 6331.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chemotaxis protein CheA (EC:2.7.13.3)
    Gene namesi
    Name:cheA
    Ordered Locus Names:TM_0702
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    ProteomesiUP000008183: Chromosome

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 671671Chemotaxis protein CheAPRO_0000074718Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei45 – 451Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-29071N.
    IntActiQ56310. 1 interaction.
    STRINGi243274.TM0702.

    Structurei

    Secondary structure

    1
    671
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 2926
    Helixi34 – 5320
    Helixi57 – 7418
    Helixi82 – 10221
    Helixi114 – 12310
    Beta strandi177 – 1848
    Helixi192 – 20514
    Beta strandi209 – 2157
    Helixi217 – 2215
    Beta strandi225 – 23713
    Helixi239 – 2479
    Beta strandi249 – 2513
    Beta strandi253 – 2608
    Beta strandi295 – 2995
    Helixi300 – 32223
    Beta strandi354 – 3574
    Helixi359 – 3624
    Helixi365 – 37511
    Beta strandi380 – 3856
    Beta strandi390 – 3923
    Helixi393 – 41321
    Helixi418 – 4247
    Beta strandi428 – 43912
    Beta strandi442 – 4498
    Helixi456 – 46510
    Beta strandi466 – 4683
    Helixi471 – 4744
    Helixi479 – 4835
    Helixi484 – 4874
    Turni489 – 4924
    Helixi493 – 4986
    Helixi499 – 5013
    Helixi506 – 51611
    Beta strandi520 – 5267
    Turni527 – 5293
    Beta strandi530 – 5389
    Beta strandi542 – 55110
    Beta strandi554 – 5596
    Helixi560 – 5623
    Beta strandi563 – 5675
    Helixi571 – 5733
    Beta strandi575 – 5773
    Beta strandi580 – 5856
    Beta strandi588 – 5947
    Helixi595 – 5984
    Beta strandi611 – 6177
    Beta strandi620 – 63718
    Helixi641 – 6444
    Beta strandi648 – 6558
    Beta strandi657 – 6593
    Beta strandi661 – 6655
    Helixi667 – 6693

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1B3QX-ray2.60A/B293-671[»]
    1I58X-ray1.60A/B352-540[»]
    1I59X-ray1.80A/B352-540[»]
    1I5AX-ray1.90A/B352-540[»]
    1I5BX-ray1.94A/B352-540[»]
    1I5CX-ray1.90A/B352-540[»]
    1I5DX-ray2.90A350-540[»]
    1TQGX-ray0.98A4-104[»]
    1U0SX-ray1.90A175-260[»]
    2CH4X-ray3.50A/B355-671[»]
    2LD6NMR-A1-131[»]
    3UR1X-ray4.50A355-671[»]
    4JPBX-ray3.19A355-671[»]
    ProteinModelPortaliQ56310.
    SMRiQ56310. Positions 4-104, 175-260, 293-671.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ56310.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 102102HPtPROSITE-ProRule annotationAdd
    BLAST
    Domaini291 – 541251Histidine kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini543 – 671129CheW-likePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 cheW-like domain.PROSITE-ProRule annotation
    Contains 1 histidine kinase domain.PROSITE-ProRule annotation
    Contains 1 HPt domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0643.
    KOiK03407.
    OMAiKISTPPM.
    OrthoDBiEOG62RS8P.

    Family and domain databases

    Gene3Di1.10.287.560. 1 hit.
    1.20.120.160. 1 hit.
    3.30.565.10. 1 hit.
    3.30.70.1110. 1 hit.
    InterProiIPR004105. CheA-like_dim.
    IPR002545. CheW.
    IPR015162. CheY-binding.
    IPR003594. HATPase_ATP-bd.
    IPR004358. Sig_transdc_His_kin-like_C.
    IPR008207. Sig_transdc_His_kin_Hpt_dom.
    IPR005467. Sig_transdc_His_kinase_core.
    IPR009082. Sig_transdc_His_kinase_dimeric.
    IPR010808. Sig_transdc_His_kinase_P2-bd.
    [Graphical view]
    PfamiPF01584. CheW. 1 hit.
    PF02895. H-kinase_dim. 1 hit.
    PF02518. HATPase_c. 1 hit.
    PF01627. Hpt. 1 hit.
    PF07194. P2. 1 hit.
    [Graphical view]
    PRINTSiPR00344. BCTRLSENSOR.
    SMARTiSM00260. CheW. 1 hit.
    SM00387. HATPase_c. 1 hit.
    SM00073. HPT. 1 hit.
    [Graphical view]
    SUPFAMiSSF47226. SSF47226. 1 hit.
    SSF47384. SSF47384. 1 hit.
    SSF50341. SSF50341. 1 hit.
    SSF55052. SSF55052. 1 hit.
    SSF55874. SSF55874. 1 hit.
    PROSITEiPS50851. CHEW. 1 hit.
    PS50109. HIS_KIN. 1 hit.
    PS50894. HPT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q56310-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMEEYLGVFV DETKEYLQNL NDTLLELEKN PEDMELINEA FRALHTLKGM    50
    AGTMGFSSMA KLCHTLENIL DKARNSEIKI TSDLLDKIFA GVDMITRMVD 100
    KIVSEGSDDI GENIDVFSDT IKSFASSGKE KPSEIKNETE TKGEEEHKGE 150
    STSNEEVVVL PEEVAHVLQE ARNKGFKTFY IKVILKEGTQ LKSARIYLVF 200
    HKLEELKCEV VRTIPSVEEI EEEKFENEVE LFVISPVDLE KLSEALSSIA 250
    DIERVIIKEV TAVTEESGAE KRTEKEEKTE KTEEKAERKK VISQTVRVDI 300
    EKLDNLMDLM GELVIARSRI LETLKKYNIK ELDESLSHLS RITLDLQNVV 350
    MKIRMVPISF VFNRFPRMVR DLAKKMNKEV NFIMRGEDTE LDRTFVEEIG 400
    EPLLHLLRNA IDHGIEPKEE RIAKGKPPIG TLILSARHEG NNVVIEVEDD 450
    GRGIDKEKII RKAIEKGLID ESKAATLSDQ EILNFLFVPG FSTKEKVSEV 500
    SGRGVGMDVV KNVVESLNGS ISIESEKDKG TKVTIRLPLT LAIIQALLVK 550
    VNNLVYAIPI ANIDTILSIS KEDIQRVQDR DVIVIRGEVI PVYRLWEVLQ 600
    IEHKEELEEM EAVIVRVGNR KYGIVVDDLL GQDDIVIKSL GKVFSEVKEF 650
    SGAAILGDGS IALIINVSGI V 671
    Length:671
    Mass (Da):75,556
    Last modified:May 30, 2000 - v2
    Checksum:iF264398B88DA34E1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti304 – 3041D → G in AAA96387. (PubMed:8550470)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U30501 Genomic DNA. Translation: AAA96387.1.
    AE000512 Genomic DNA. Translation: AAD35784.1.
    PIRiD72346.
    RefSeqiNP_228511.1. NC_000853.1.
    YP_007977052.1. NC_021214.1.
    YP_008990329.1. NC_023151.1.

    Genome annotation databases

    EnsemblBacteriaiAAD35784; AAD35784; TM_0702.
    GeneIDi898369.
    KEGGitma:TM0702.
    tmi:THEMA_01155.
    tmm:Tmari_0702.
    PATRICi23936322. VBITheMar51294_0714.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U30501 Genomic DNA. Translation: AAA96387.1 .
    AE000512 Genomic DNA. Translation: AAD35784.1 .
    PIRi D72346.
    RefSeqi NP_228511.1. NC_000853.1.
    YP_007977052.1. NC_021214.1.
    YP_008990329.1. NC_023151.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1B3Q X-ray 2.60 A/B 293-671 [» ]
    1I58 X-ray 1.60 A/B 352-540 [» ]
    1I59 X-ray 1.80 A/B 352-540 [» ]
    1I5A X-ray 1.90 A/B 352-540 [» ]
    1I5B X-ray 1.94 A/B 352-540 [» ]
    1I5C X-ray 1.90 A/B 352-540 [» ]
    1I5D X-ray 2.90 A 350-540 [» ]
    1TQG X-ray 0.98 A 4-104 [» ]
    1U0S X-ray 1.90 A 175-260 [» ]
    2CH4 X-ray 3.50 A/B 355-671 [» ]
    2LD6 NMR - A 1-131 [» ]
    3UR1 X-ray 4.50 A 355-671 [» ]
    4JPB X-ray 3.19 A 355-671 [» ]
    ProteinModelPortali Q56310.
    SMRi Q56310. Positions 4-104, 175-260, 293-671.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-29071N.
    IntActi Q56310. 1 interaction.
    STRINGi 243274.TM0702.

    Protocols and materials databases

    DNASUi 898369.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD35784 ; AAD35784 ; TM_0702 .
    GeneIDi 898369.
    KEGGi tma:TM0702.
    tmi:THEMA_01155.
    tmm:Tmari_0702.
    PATRICi 23936322. VBITheMar51294_0714.

    Phylogenomic databases

    eggNOGi COG0643.
    KOi K03407.
    OMAi KISTPPM.
    OrthoDBi EOG62RS8P.

    Enzyme and pathway databases

    BRENDAi 2.7.13.3. 6331.

    Miscellaneous databases

    EvolutionaryTracei Q56310.

    Family and domain databases

    Gene3Di 1.10.287.560. 1 hit.
    1.20.120.160. 1 hit.
    3.30.565.10. 1 hit.
    3.30.70.1110. 1 hit.
    InterProi IPR004105. CheA-like_dim.
    IPR002545. CheW.
    IPR015162. CheY-binding.
    IPR003594. HATPase_ATP-bd.
    IPR004358. Sig_transdc_His_kin-like_C.
    IPR008207. Sig_transdc_His_kin_Hpt_dom.
    IPR005467. Sig_transdc_His_kinase_core.
    IPR009082. Sig_transdc_His_kinase_dimeric.
    IPR010808. Sig_transdc_His_kinase_P2-bd.
    [Graphical view ]
    Pfami PF01584. CheW. 1 hit.
    PF02895. H-kinase_dim. 1 hit.
    PF02518. HATPase_c. 1 hit.
    PF01627. Hpt. 1 hit.
    PF07194. P2. 1 hit.
    [Graphical view ]
    PRINTSi PR00344. BCTRLSENSOR.
    SMARTi SM00260. CheW. 1 hit.
    SM00387. HATPase_c. 1 hit.
    SM00073. HPT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47226. SSF47226. 1 hit.
    SSF47384. SSF47384. 1 hit.
    SSF50341. SSF50341. 1 hit.
    SSF55052. SSF55052. 1 hit.
    SSF55874. SSF55874. 1 hit.
    PROSITEi PS50851. CHEW. 1 hit.
    PS50109. HIS_KIN. 1 hit.
    PS50894. HPT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Thermostable chemotaxis proteins from the hyperthermophilic bacterium Thermotoga maritima."
      Swanson R.V., Sanna M.G., Simon M.I.
      J. Bacteriol. 178:484-489(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
    3. "Structure of CheA, a signal-transducing histidine kinase."
      Bilwes A.M., Alex L.A., Crane B.R., Simon M.I.
      Cell 96:131-141(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 293-671.

    Entry informationi

    Entry nameiCHEA_THEMA
    AccessioniPrimary (citable) accession number: Q56310
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3