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Protein

Protein-L-isoaspartate O-methyltransferase

Gene

pcm

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins.

Catalytic activityi

S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester.

Kineticsi

    1. Vmax=164 nmol/min/mg enzyme with KASA (isoD) LAKY as substrate at 85 degrees Celsius

    Temperature dependencei

    Optimum temperature is 85 degrees Celsius. Highly thermostable, with no loss of activity after 60 min at 100 degrees Celsius. Enzyme activity is observed at temperatures as high as 93 degrees Celsius.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei59By similarity1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein-L-isoaspartate O-methyltransferase (EC:2.1.1.77)
    Alternative name(s):
    L-isoaspartyl protein carboxyl methyltransferase
    Protein L-isoaspartyl methyltransferase
    Protein-beta-aspartate methyltransferase
    Short name:
    PIMT
    Gene namesi
    Name:pcm
    Ordered Locus Names:TM_0704
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    Proteomesi
    • UP000008183 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001119051 – 317Protein-L-isoaspartate O-methyltransferaseAdd BLAST317

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    STRINGi243274.TM0704.

    Structurei

    Secondary structure

    1317
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi2 – 11Combined sources10
    Helixi16 – 24Combined sources9
    Helixi27 – 30Combined sources4
    Helixi37 – 40Combined sources4
    Beta strandi42 – 44Combined sources3
    Beta strandi46 – 50Combined sources5
    Beta strandi55 – 58Combined sources4
    Helixi61 – 70Combined sources10
    Beta strandi78 – 82Combined sources5
    Helixi88 – 97Combined sources10
    Beta strandi102 – 108Combined sources7
    Helixi110 – 122Combined sources13
    Beta strandi127 – 133Combined sources7
    Helixi135 – 137Combined sources3
    Helixi140 – 142Combined sources3
    Beta strandi145 – 150Combined sources6
    Beta strandi152 – 155Combined sources4
    Helixi159 – 164Combined sources6
    Beta strandi165 – 175Combined sources11
    Helixi178 – 180Combined sources3
    Beta strandi184 – 191Combined sources8
    Beta strandi194 – 202Combined sources9
    Helixi210 – 212Combined sources3
    Helixi215 – 220Combined sources6
    Beta strandi228 – 233Combined sources6
    Helixi237 – 248Combined sources12
    Beta strandi251 – 254Combined sources4
    Beta strandi257 – 261Combined sources5
    Beta strandi263 – 270Combined sources8
    Beta strandi273 – 278Combined sources6
    Helixi281 – 292Combined sources12
    Turni293 – 295Combined sources3
    Helixi298 – 300Combined sources3
    Beta strandi301 – 309Combined sources9
    Beta strandi312 – 314Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1DL5X-ray1.80A/B1-317[»]
    ProteinModelPortaliQ56308.
    SMRiQ56308.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ56308.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105E26. Bacteria.
    COG2518. LUCA.
    InParanoidiQ56308.
    KOiK00573.
    OMAiFARILVT.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    3.55.20.10. 1 hit.
    HAMAPiMF_00090. PIMT. 1 hit.
    InterProiIPR000682. PCMT.
    IPR009107. PIM_MeTrfase_C.
    IPR029063. SAM-dependent_MTases.
    [Graphical view]
    PANTHERiPTHR11579. PTHR11579. 1 hit.
    SUPFAMiSSF53335. SSF53335. 2 hits.
    SSF68930. SSF68930. 1 hit.
    PROSITEiPS01279. PCMT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q56308-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MREKLFWILK KYGVSDHIAK AFLEIPREEF LTKSYPLSYV YEDIVLVSYD
    60 70 80 90 100
    DGEEYSTSSQ PSLMALFMEW VGLDKGMRVL EIGGGTGYNA AVMSRVVGEK
    110 120 130 140 150
    GLVVSVEYSR KICEIAKRNV ERLGIENVIF VCGDGYYGVP EFSPYDVIFV
    160 170 180 190 200
    TVGVDEVPET WFTQLKEGGR VIVPINLKLS RRQPAFLFKK KDPYLVGNYK
    210 220 230 240 250
    LETRFITAGG NLGNLLERNR KLLREFPFNR EILLVRSHIF VELVDLLTRR
    260 270 280 290 300
    LTEIDGTFYY AGPNGVVEFL DDRMRIYGDA PEIENLLTQW ESCGYRSFEY
    310
    LMLHVGYNAF SHISCSI
    Length:317
    Mass (Da):36,400
    Last modified:November 1, 1996 - v1
    Checksum:i2FE6019571ADDF2C
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U30501 Genomic DNA. Translation: AAA96385.1.
    AE000512 Genomic DNA. Translation: AAD35786.1.
    PIRiG72342.
    RefSeqiNP_228513.1. NC_000853.1.
    WP_004081036.1. NZ_CP011107.1.

    Genome annotation databases

    EnsemblBacteriaiAAD35786; AAD35786; TM_0704.
    GeneIDi898371.
    KEGGitma:TM0704.
    PATRICi23936326. VBITheMar51294_0716.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U30501 Genomic DNA. Translation: AAA96385.1.
    AE000512 Genomic DNA. Translation: AAD35786.1.
    PIRiG72342.
    RefSeqiNP_228513.1. NC_000853.1.
    WP_004081036.1. NZ_CP011107.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1DL5X-ray1.80A/B1-317[»]
    ProteinModelPortaliQ56308.
    SMRiQ56308.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi243274.TM0704.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAD35786; AAD35786; TM_0704.
    GeneIDi898371.
    KEGGitma:TM0704.
    PATRICi23936326. VBITheMar51294_0716.

    Phylogenomic databases

    eggNOGiENOG4105E26. Bacteria.
    COG2518. LUCA.
    InParanoidiQ56308.
    KOiK00573.
    OMAiFARILVT.

    Miscellaneous databases

    EvolutionaryTraceiQ56308.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    3.55.20.10. 1 hit.
    HAMAPiMF_00090. PIMT. 1 hit.
    InterProiIPR000682. PCMT.
    IPR009107. PIM_MeTrfase_C.
    IPR029063. SAM-dependent_MTases.
    [Graphical view]
    PANTHERiPTHR11579. PTHR11579. 1 hit.
    SUPFAMiSSF53335. SSF53335. 2 hits.
    SSF68930. SSF68930. 1 hit.
    PROSITEiPS01279. PCMT. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPIMT_THEMA
    AccessioniPrimary (citable) accession number: Q56308
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: November 2, 2016
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.