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Q56308

- PIMT_THEMA

UniProt

Q56308 - PIMT_THEMA

Protein

Protein-L-isoaspartate O-methyltransferase

Gene

pcm

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins.

    Catalytic activityi

    S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester.

    Kineticsi

      Vmax=164 nmol/min/mg enzyme with KASA (isoD) LAKY as substrate at 85 degrees Celsius

      Temperature dependencei

      Optimum temperature is 85 degrees Celsius. Highly thermostable, with no loss of activity after 60 min at 100 degrees Celsius. Enzyme activity is observed at temperatures as high as 93 degrees Celsius.

      Sites

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Active sitei59 – 591By similarity

      GO - Molecular functioni

      1. protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Source: UniProtKB-HAMAP

      GO - Biological processi

      1. protein repair Source: UniProtKB-HAMAP

      Keywords - Molecular functioni

      Methyltransferase, Transferase

      Keywords - Ligandi

      S-adenosyl-L-methionine

      Names & Taxonomyi

      Protein namesi
      Recommended name:
      Protein-L-isoaspartate O-methyltransferase (EC:2.1.1.77)
      Alternative name(s):
      L-isoaspartyl protein carboxyl methyltransferase
      Protein L-isoaspartyl methyltransferase
      Protein-beta-aspartate methyltransferase
      Short name:
      PIMT
      Gene namesi
      Name:pcm
      Ordered Locus Names:TM_0704
      OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
      Taxonomic identifieri243274 [NCBI]
      Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
      ProteomesiUP000008183: Chromosome

      Subcellular locationi

      Cytoplasm By similarity

      GO - Cellular componenti

      1. cytoplasm Source: UniProtKB-SubCell

      Keywords - Cellular componenti

      Cytoplasm

      PTM / Processingi

      Molecule processing

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Chaini1 – 317317Protein-L-isoaspartate O-methyltransferasePRO_0000111905Add
      BLAST

      Interactioni

      Subunit structurei

      Monomer.

      Protein-protein interaction databases

      STRINGi243274.TM0704.

      Structurei

      Secondary structure

      1
      317
      Legend: HelixTurnBeta strand
      Show more details
      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Helixi2 – 1110
      Helixi16 – 249
      Helixi27 – 304
      Helixi37 – 404
      Beta strandi42 – 443
      Beta strandi46 – 505
      Beta strandi55 – 584
      Helixi61 – 7010
      Beta strandi78 – 825
      Helixi88 – 9710
      Beta strandi102 – 1087
      Helixi110 – 12213
      Beta strandi127 – 1337
      Helixi135 – 1373
      Helixi140 – 1423
      Beta strandi145 – 1506
      Beta strandi152 – 1554
      Helixi159 – 1646
      Beta strandi165 – 17511
      Helixi178 – 1803
      Beta strandi184 – 1918
      Beta strandi194 – 2029
      Helixi210 – 2123
      Helixi215 – 2206
      Beta strandi228 – 2336
      Helixi237 – 24812
      Beta strandi251 – 2544
      Beta strandi257 – 2615
      Beta strandi263 – 2708
      Beta strandi273 – 2786
      Helixi281 – 29212
      Turni293 – 2953
      Helixi298 – 3003
      Beta strandi301 – 3099
      Beta strandi312 – 3143

      3D structure databases

      Select the link destinations:
      PDBe
      RCSB PDB
      PDBj
      Links Updated
      EntryMethodResolution (Å)ChainPositionsPDBsum
      1DL5X-ray1.80A/B1-317[»]
      ProteinModelPortaliQ56308.
      SMRiQ56308. Positions 1-317.
      ModBaseiSearch...
      MobiDBiSearch...

      Miscellaneous databases

      EvolutionaryTraceiQ56308.

      Family & Domainsi

      Sequence similaritiesi

      Phylogenomic databases

      eggNOGiCOG2518.
      KOiK00573.
      OMAiPTQWRES.
      OrthoDBiEOG644ZP2.

      Family and domain databases

      Gene3Di3.40.50.150. 1 hit.
      3.55.20.10. 1 hit.
      HAMAPiMF_00090. PIMT.
      InterProiIPR000682. PCMT.
      IPR009107. PIM_MeTrfase_C.
      IPR029063. SAM-dependent_MTases-like.
      [Graphical view]
      PANTHERiPTHR11579. PTHR11579. 1 hit.
      PfamiPF01135. PCMT. 1 hit.
      [Graphical view]
      SUPFAMiSSF53335. SSF53335. 2 hits.
      SSF68930. SSF68930. 1 hit.
      PROSITEiPS01279. PCMT. 1 hit.
      [Graphical view]

      Sequencei

      Sequence statusi: Complete.

      Q56308-1 [UniParc]FASTAAdd to Basket

      « Hide

      MREKLFWILK KYGVSDHIAK AFLEIPREEF LTKSYPLSYV YEDIVLVSYD    50
      DGEEYSTSSQ PSLMALFMEW VGLDKGMRVL EIGGGTGYNA AVMSRVVGEK 100
      GLVVSVEYSR KICEIAKRNV ERLGIENVIF VCGDGYYGVP EFSPYDVIFV 150
      TVGVDEVPET WFTQLKEGGR VIVPINLKLS RRQPAFLFKK KDPYLVGNYK 200
      LETRFITAGG NLGNLLERNR KLLREFPFNR EILLVRSHIF VELVDLLTRR 250
      LTEIDGTFYY AGPNGVVEFL DDRMRIYGDA PEIENLLTQW ESCGYRSFEY 300
      LMLHVGYNAF SHISCSI 317
      Length:317
      Mass (Da):36,400
      Last modified:November 1, 1996 - v1
      Checksum:i2FE6019571ADDF2C
      GO

      Sequence databases

      Select the link destinations:
      EMBL
      GenBank
      DDBJ
      Links Updated
      U30501 Genomic DNA. Translation: AAA96385.1.
      AE000512 Genomic DNA. Translation: AAD35786.1.
      PIRiG72342.
      RefSeqiNP_228513.1. NC_000853.1.
      YP_007977054.1. NC_021214.1.
      YP_008990327.1. NC_023151.1.

      Genome annotation databases

      EnsemblBacteriaiAAD35786; AAD35786; TM_0704.
      GeneIDi898371.
      KEGGitma:TM0704.
      tmi:THEMA_01145.
      tmm:Tmari_0704.
      PATRICi23936326. VBITheMar51294_0716.

      Cross-referencesi

      Sequence databases

      Select the link destinations:
      EMBL
      GenBank
      DDBJ
      Links Updated
      U30501 Genomic DNA. Translation: AAA96385.1 .
      AE000512 Genomic DNA. Translation: AAD35786.1 .
      PIRi G72342.
      RefSeqi NP_228513.1. NC_000853.1.
      YP_007977054.1. NC_021214.1.
      YP_008990327.1. NC_023151.1.

      3D structure databases

      Select the link destinations:
      PDBe
      RCSB PDB
      PDBj
      Links Updated
      Entry Method Resolution (Å) Chain Positions PDBsum
      1DL5 X-ray 1.80 A/B 1-317 [» ]
      ProteinModelPortali Q56308.
      SMRi Q56308. Positions 1-317.
      ModBasei Search...
      MobiDBi Search...

      Protein-protein interaction databases

      STRINGi 243274.TM0704.

      Protocols and materials databases

      Structural Biology Knowledgebase Search...

      Genome annotation databases

      EnsemblBacteriai AAD35786 ; AAD35786 ; TM_0704 .
      GeneIDi 898371.
      KEGGi tma:TM0704.
      tmi:THEMA_01145.
      tmm:Tmari_0704.
      PATRICi 23936326. VBITheMar51294_0716.

      Phylogenomic databases

      eggNOGi COG2518.
      KOi K00573.
      OMAi PTQWRES.
      OrthoDBi EOG644ZP2.

      Miscellaneous databases

      EvolutionaryTracei Q56308.

      Family and domain databases

      Gene3Di 3.40.50.150. 1 hit.
      3.55.20.10. 1 hit.
      HAMAPi MF_00090. PIMT.
      InterProi IPR000682. PCMT.
      IPR009107. PIM_MeTrfase_C.
      IPR029063. SAM-dependent_MTases-like.
      [Graphical view ]
      PANTHERi PTHR11579. PTHR11579. 1 hit.
      Pfami PF01135. PCMT. 1 hit.
      [Graphical view ]
      SUPFAMi SSF53335. SSF53335. 2 hits.
      SSF68930. SSF68930. 1 hit.
      PROSITEi PS01279. PCMT. 1 hit.
      [Graphical view ]
      ProtoNeti Search...

      Publicationsi

      1. "Thermostable chemotaxis proteins from the hyperthermophilic bacterium Thermotoga maritima."
        Swanson R.V., Sanna M.G., Simon M.I.
        J. Bacteriol. 178:484-489(1996) [PubMed] [Europe PMC] [Abstract]
        Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
        Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
      3. "A highly active protein repair enzyme from an extreme thermophile: the L-isoaspartyl methyltransferase from Thermotoga maritima."
        Ichikawa J.K., Clarke S.
        Arch. Biochem. Biophys. 358:222-231(1998) [PubMed] [Europe PMC] [Abstract]
        Cited for: CHARACTERIZATION.
      4. "Crystal structure of protein isoaspartyl methyltransferase. A catalyst for protein repair."
        Skinner M.M., Puvathingal J.M., Walter R.L., Friedman A.M.
        Structure 8:1189-1201(2000) [PubMed] [Europe PMC] [Abstract]
        Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).

      Entry informationi

      Entry nameiPIMT_THEMA
      AccessioniPrimary (citable) accession number: Q56308
      Entry historyi
      Integrated into UniProtKB/Swiss-Prot: November 1, 1997
      Last sequence update: November 1, 1996
      Last modified: October 1, 2014
      This is version 106 of the entry and version 1 of the sequence. [Complete history]
      Entry statusiReviewed (UniProtKB/Swiss-Prot)
      Annotation programProkaryotic Protein Annotation Program

      Miscellaneousi

      Keywords - Technical termi

      3D-structure, Complete proteome, Reference proteome

      Documents

      1. PDB cross-references
        Index of Protein Data Bank (PDB) cross-references
      2. SIMILARITY comments
        Index of protein domains and families

      External Data

      Dasty 3