ID   BGAL_THEMA              Reviewed;        1084 AA.
AC   Q56307; O33834;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 2.
DT   27-MAR-2024, entry version 155.
DE   RecName: Full=Beta-galactosidase;
DE            Short=Beta-gal;
DE            EC=3.2.1.23;
DE   AltName: Full=Lactase;
GN   Name=lacZ; OrderedLocusNames=TM_1193;
OS   Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826
OS   / MSB8).
OC   Bacteria; Thermotogota; Thermotogae; Thermotogales; Thermotogaceae;
OC   Thermotoga.
OX   NCBI_TaxID=243274;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX   PubMed=8088532; DOI=10.1016/0378-1119(94)90046-9;
RA   Moore J.B., Markiewicz P., Miller J.H.;
RT   "Identification and sequencing of the Thermotoga maritima lacZ gene, part
RT   of a divergently transcribed operon.";
RL   Gene 147:101-106(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX   PubMed=10360571; DOI=10.1038/20601;
RA   Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H.,
RA   Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A.,
RA   Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M.,
RA   Cotton M.D., Pratt M.S., Phillips C.A., Richardson D.L., Heidelberg J.F.,
RA   Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L.,
RA   Smith H.O., Venter J.C., Fraser C.M.;
RT   "Evidence for lateral gene transfer between Archaea and Bacteria from
RT   genome sequence of Thermotoga maritima.";
RL   Nature 399:323-329(1999).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 554-1084.
RC   STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX   PubMed=9741105; DOI=10.1016/s0723-2020(98)80002-7;
RA   Liebl W., Wagner B., Schellhase J.;
RT   "Properties of an alpha-galactosidase, and structure of its gene galA,
RT   within an alpha- and beta-galactoside utilization gene cluster of the
RT   hyperthermophilic bacterium Thermotoga maritima.";
RL   Syst. Appl. Microbiol. 21:1-11(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC         in beta-D-galactosides.; EC=3.2.1.23;
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAD36268.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; U08186; AAA50597.1; -; Genomic_DNA.
DR   EMBL; AE000512; AAD36268.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AJ001072; CAA04513.1; -; Genomic_DNA.
DR   PIR; F72283; F72283.
DR   RefSeq; NP_228998.1; NC_000853.1.
DR   RefSeq; WP_004080135.1; NZ_CP011107.1.
DR   PDB; 6S6Z; EM; 2.10 A; A/B/C/D/E/F/G/H=2-1084.
DR   PDB; 6SD0; X-ray; 3.70 A; A/B/C/D=1-1084.
DR   PDBsum; 6S6Z; -.
DR   PDBsum; 6SD0; -.
DR   AlphaFoldDB; Q56307; -.
DR   EMDB; EMD-10109; -.
DR   SMR; Q56307; -.
DR   STRING; 243274.TM_1193; -.
DR   CAZy; GH2; Glycoside Hydrolase Family 2.
DR   PaxDb; 243274-THEMA_08365; -.
DR   EnsemblBacteria; AAD36268; AAD36268; TM_1193.
DR   KEGG; tma:TM1193; -.
DR   PATRIC; fig|243274.5.peg.1211; -.
DR   eggNOG; COG3250; Bacteria.
DR   InParanoid; Q56307; -.
DR   OrthoDB; 9762066at2; -.
DR   Proteomes; UP000008183; Chromosome.
DR   GO; GO:0009341; C:beta-galactosidase complex; IBA:GO_Central.
DR   GO; GO:0004565; F:beta-galactosidase activity; IBA:GO_Central.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR   GO; GO:0005990; P:lactose catabolic process; IBA:GO_Central.
DR   Gene3D; 2.70.98.10; -; 1.
DR   Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR   InterPro; IPR004199; B-gal_small/dom_5.
DR   InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR   InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR014718; GH-type_carb-bd.
DR   InterPro; IPR006101; Glyco_hydro_2.
DR   InterPro; IPR023232; Glyco_hydro_2_AS.
DR   InterPro; IPR006103; Glyco_hydro_2_cat.
DR   InterPro; IPR023230; Glyco_hydro_2_CS.
DR   InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR   InterPro; IPR006104; Glyco_hydro_2_N.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR032312; LacZ_4.
DR   NCBIfam; NF041100; betagal_LacZ_Ttogales; 1.
DR   PANTHER; PTHR46323; BETA-GALACTOSIDASE; 1.
DR   PANTHER; PTHR46323:SF2; BETA-GALACTOSIDASE; 1.
DR   Pfam; PF02929; Bgal_small_N; 1.
DR   Pfam; PF00703; Glyco_hydro_2; 1.
DR   Pfam; PF02836; Glyco_hydro_2_C; 1.
DR   Pfam; PF02837; Glyco_hydro_2_N; 1.
DR   Pfam; PF16353; LacZ_4; 1.
DR   PRINTS; PR00132; GLHYDRLASE2.
DR   SMART; SM01038; Bgal_small_N; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 2.
DR   SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR   SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR   PROSITE; PS00719; GLYCOSYL_HYDROL_F2_1; 1.
DR   PROSITE; PS00608; GLYCOSYL_HYDROL_F2_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Glycosidase; Hydrolase; Reference proteome.
FT   CHAIN           1..1084
FT                   /note="Beta-galactosidase"
FT                   /id="PRO_0000057678"
FT   ACT_SITE        441
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        507
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        152..175
FT                   /note="SKDSCTPAEFRLTDVLRPGKNLIT -> RQRQLHARRIQTHRCSKTREESDH
FT                   (in Ref. 1; AAA50597)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1028..1084
FT                   /note="SKEKQVVLFVDGNEYSVRRVVIPPFKKEELVFKVEGLKKGEHLIHTNLNTRK
FT                   TIYVR -> RQGKTGGSLC (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   HELIX           4..6
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          12..14
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          23..26
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   TURN            28..30
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          37..41
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          44..53
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   HELIX           54..56
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   TURN            59..62
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          63..65
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          71..77
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   HELIX           80..82
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          89..94
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          106..108
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          111..120
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   HELIX           122..126
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          127..134
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          137..144
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          147..152
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          159..162
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   TURN            164..166
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          169..180
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   HELIX           183..187
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          191..194
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          202..207
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          209..221
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          227..240
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          245..252
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          258..266
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          271..281
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          287..290
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          293..299
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          302..308
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          313..316
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          319..322
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          329..333
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   TURN            339..341
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   HELIX           347..358
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   TURN            359..361
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          364..366
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   HELIX           374..383
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          386..390
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   TURN            402..404
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   HELIX           406..408
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   HELIX           410..412
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   HELIX           413..427
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          433..437
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   HELIX           446..458
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          461..465
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   HELIX           467..470
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          479..485
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   HELIX           488..495
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          503..509
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          512..514
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   HELIX           519..526
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          532..538
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          545..548
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          554..557
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          561..563
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   TURN            569..572
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   HELIX           586..594
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          596..603
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          606..611
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   HELIX           618..620
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          621..629
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          632..639
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          648..652
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          663..673
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          676..678
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          683..692
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          697..699
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          706..709
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          711..718
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          721..726
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   TURN            727..729
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          731..737
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          744..750
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   HELIX           757..761
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   HELIX           764..767
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   HELIX           769..774
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          779..787
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          789..799
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          805..813
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          818..825
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          834..843
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          848..857
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          868..875
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   HELIX           876..878
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          892..901
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          906..922
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   HELIX           924..929
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   HELIX           933..935
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          940..950
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          958..960
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   HELIX           965..967
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          974..985
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          991..993
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          1000..1004
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          1007..1009
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          1015..1023
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          1025..1027
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          1029..1037
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          1040..1049
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          1053..1061
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          1066..1073
FT                   /evidence="ECO:0007829|PDB:6S6Z"
FT   STRAND          1078..1083
FT                   /evidence="ECO:0007829|PDB:6S6Z"
SQ   SEQUENCE   1084 AA;  127608 MW;  D52E3B762B53DDFC CRC64;
     MPYEWENPQL VSEGTEKSHA SFIPYLDPFS GEWEYPEEFI SLNGNWRFLF AKNPFEVPED
     FFSEKFDDSN WDEIEVPSNW EMKGYGKPIY TNVVYPFEPN PPFVPKDDNP TGVYRRWIEI
     PEDWFKKEIF LHFEGVRSFF YLWVNGKKIG FSKDSCTPAE FRLTDVLRPG KNLITVEVLK
     WSDGSYLEDQ DMWWFAGIYR DVYLYALPKF HIRDVFVRTD LDENYRNGKI FLDVEMRNLG
     EEEEKDLEVT LITPDGDEKT LVKETVKPED RVLSFAFDVK DPKKWSAETP HLYVLKLKLG
     EDEKKVNFGF RKIEIKDGTL LFNGKPLYIK GVNRHEFDPD RGHAVTVERM IQDIKLMKQH
     NINTVRTSHY PNQTKWYDLC DYFGLYVIDE ANIESHGIDW DPEVTLANRW EWEKAHFDRI
     KRMVERDKNH PSIIFWSLGN EAGDGVNFEK AALWIKKRDN TRLIHYEGTT RRGESYYVDV
     FSLMYPKMDI LLEYASKKRE KPFIMCEYAH AMGNSVGNLK DYWDVIEKYP YLHGGCIWDW
     VDQGIRKKDE NGREFWAYGG DFGDTPNDGN FCINGVVLPD RTPEPELYEV KKVYQNVKIR
     QVSKDTYEVE NRYLFTNLEM FDGAWKIRKD GEVIEEKTFK IFAEPGEKRL LKIPLPEMDD
     SEYFLEISFS LSEDTPWAEK GHVVAWEQFL LKAPAFEKKS ISDGVSLRED GKHLTVEAKD
     TVYVFSKLTG LLEQILHRRK KILKSPVVPN FWRVPTDNDI GNRMPQRLAI WKRASKERKL
     FKMHWKKEEN RVSVHSVFQL PGNSWVYTTY TVFGNGDVLV DLSLIPAEDV PEIPRIGFQF
     TVPEEFGTVE WYGRGPHETY WDRKESGLFA RYRKAVGEMM HRYVRPQETG NRSDVRWFAL
     SDGETKLFVS GMPQIDFSVW PFSMEDLERV QHISELPERD FVTVNVDFRQ MGLGGDDSWG
     AMPHLEYRLL PKPYRFSFRM RISEEIPSWR VLAAIPETLH VEMSSEDVIR EGDTLRVKFS
     LLNDTPLSKE KQVVLFVDGN EYSVRRVVIP PFKKEELVFK VEGLKKGEHL IHTNLNTRKT
     IYVR
//