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Q56307

- BGAL_THEMA

UniProt

Q56307 - BGAL_THEMA

Protein

Beta-galactosidase

Gene

lacZ

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei441 – 4411Proton donorBy similarity
    Active sitei507 – 5071NucleophileBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciTMAR243274:GC6P-1223-MONOMER.

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase (EC:3.2.1.23)
    Short name:
    Beta-gal
    Alternative name(s):
    Lactase
    Gene namesi
    Name:lacZ
    Ordered Locus Names:TM_1193
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    ProteomesiUP000008183: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10841084Beta-galactosidasePRO_0000057678Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi243274.TM1193.

    Structurei

    3D structure databases

    ProteinModelPortaliQ56307.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.Curated

    Phylogenomic databases

    eggNOGiCOG3250.
    KOiK01190.
    OMAiNPPFVPK.
    OrthoDBiEOG6XWV0T.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q56307-1 [UniParc]FASTAAdd to Basket

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    MPYEWENPQL VSEGTEKSHA SFIPYLDPFS GEWEYPEEFI SLNGNWRFLF     50
    AKNPFEVPED FFSEKFDDSN WDEIEVPSNW EMKGYGKPIY TNVVYPFEPN 100
    PPFVPKDDNP TGVYRRWIEI PEDWFKKEIF LHFEGVRSFF YLWVNGKKIG 150
    FSKDSCTPAE FRLTDVLRPG KNLITVEVLK WSDGSYLEDQ DMWWFAGIYR 200
    DVYLYALPKF HIRDVFVRTD LDENYRNGKI FLDVEMRNLG EEEEKDLEVT 250
    LITPDGDEKT LVKETVKPED RVLSFAFDVK DPKKWSAETP HLYVLKLKLG 300
    EDEKKVNFGF RKIEIKDGTL LFNGKPLYIK GVNRHEFDPD RGHAVTVERM 350
    IQDIKLMKQH NINTVRTSHY PNQTKWYDLC DYFGLYVIDE ANIESHGIDW 400
    DPEVTLANRW EWEKAHFDRI KRMVERDKNH PSIIFWSLGN EAGDGVNFEK 450
    AALWIKKRDN TRLIHYEGTT RRGESYYVDV FSLMYPKMDI LLEYASKKRE 500
    KPFIMCEYAH AMGNSVGNLK DYWDVIEKYP YLHGGCIWDW VDQGIRKKDE 550
    NGREFWAYGG DFGDTPNDGN FCINGVVLPD RTPEPELYEV KKVYQNVKIR 600
    QVSKDTYEVE NRYLFTNLEM FDGAWKIRKD GEVIEEKTFK IFAEPGEKRL 650
    LKIPLPEMDD SEYFLEISFS LSEDTPWAEK GHVVAWEQFL LKAPAFEKKS 700
    ISDGVSLRED GKHLTVEAKD TVYVFSKLTG LLEQILHRRK KILKSPVVPN 750
    FWRVPTDNDI GNRMPQRLAI WKRASKERKL FKMHWKKEEN RVSVHSVFQL 800
    PGNSWVYTTY TVFGNGDVLV DLSLIPAEDV PEIPRIGFQF TVPEEFGTVE 850
    WYGRGPHETY WDRKESGLFA RYRKAVGEMM HRYVRPQETG NRSDVRWFAL 900
    SDGETKLFVS GMPQIDFSVW PFSMEDLERV QHISELPERD FVTVNVDFRQ 950
    MGLGGDDSWG AMPHLEYRLL PKPYRFSFRM RISEEIPSWR VLAAIPETLH 1000
    VEMSSEDVIR EGDTLRVKFS LLNDTPLSKE KQVVLFVDGN EYSVRRVVIP 1050
    PFKKEELVFK VEGLKKGEHL IHTNLNTRKT IYVR 1084
    Length:1,084
    Mass (Da):127,608
    Last modified:May 30, 2000 - v2
    Checksum:iD52E3B762B53DDFC
    GO

    Sequence cautioni

    The sequence AAD36268.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti152 – 17524SKDSC…KNLIT → RQRQLHARRIQTHRCSKTRE ESDH in AAA50597. (PubMed:8088532)CuratedAdd
    BLAST
    Sequence conflicti1028 – 108457SKEKQ…TIYVR → RQGKTGGSLC(PubMed:8088532)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U08186 Genomic DNA. Translation: AAA50597.1.
    AE000512 Genomic DNA. Translation: AAD36268.1. Different initiation.
    AJ001072 Genomic DNA. Translation: CAA04513.1.
    PIRiF72283.
    RefSeqiNP_228998.1. NC_000853.1.
    YP_007977549.1. NC_021214.1.

    Genome annotation databases

    EnsemblBacteriaiAAD36268; AAD36268; TM_1193.
    GeneIDi898291.
    KEGGitma:TM1193.
    tmm:Tmari_1200.
    PATRICi23937328. VBITheMar51294_1211.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U08186 Genomic DNA. Translation: AAA50597.1 .
    AE000512 Genomic DNA. Translation: AAD36268.1 . Different initiation.
    AJ001072 Genomic DNA. Translation: CAA04513.1 .
    PIRi F72283.
    RefSeqi NP_228998.1. NC_000853.1.
    YP_007977549.1. NC_021214.1.

    3D structure databases

    ProteinModelPortali Q56307.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243274.TM1193.

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD36268 ; AAD36268 ; TM_1193 .
    GeneIDi 898291.
    KEGGi tma:TM1193.
    tmm:Tmari_1200.
    PATRICi 23937328. VBITheMar51294_1211.

    Phylogenomic databases

    eggNOGi COG3250.
    KOi K01190.
    OMAi NPPFVPK.
    OrthoDBi EOG6XWV0T.

    Enzyme and pathway databases

    BioCyci TMAR243274:GC6P-1223-MONOMER.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and sequencing of the Thermotoga maritima lacZ gene, part of a divergently transcribed operon."
      Moore J.B., Markiewicz P., Miller J.H.
      Gene 147:101-106(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
    3. "Properties of an alpha-galactosidase, and structure of its gene galA, within an alpha- and beta-galactoside utilization gene cluster of the hyperthermophilic bacterium Thermotoga maritima."
      Liebl W., Wagner B., Schellhase J.
      Syst. Appl. Microbiol. 21:1-11(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 554-1084.
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

    Entry informationi

    Entry nameiBGAL_THEMA
    AccessioniPrimary (citable) accession number: Q56307
    Secondary accession number(s): O33834
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 103 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3