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Q56307 (BGAL_THEMA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:lacZ
Ordered Locus Names:TM_1193
OrganismThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [Reference proteome] [HAMAP]
Taxonomic identifier243274 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

Protein attributes

Sequence length1084 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Sequence caution

The sequence AAD36268.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentbeta-galactosidase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10841084Beta-galactosidase
PRO_0000057678

Sites

Active site4411Proton donor By similarity
Active site5071Nucleophile By similarity

Experimental info

Sequence conflict152 – 17524SKDSC…KNLIT → RQRQLHARRIQTHRCSKTRE ESDH in AAA50597. Ref.1
Sequence conflict1028 – 108457SKEKQ…TIYVR → RQGKTGGSLC Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q56307 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: D52E3B762B53DDFC

FASTA1,084127,608
        10         20         30         40         50         60 
MPYEWENPQL VSEGTEKSHA SFIPYLDPFS GEWEYPEEFI SLNGNWRFLF AKNPFEVPED 

        70         80         90        100        110        120 
FFSEKFDDSN WDEIEVPSNW EMKGYGKPIY TNVVYPFEPN PPFVPKDDNP TGVYRRWIEI 

       130        140        150        160        170        180 
PEDWFKKEIF LHFEGVRSFF YLWVNGKKIG FSKDSCTPAE FRLTDVLRPG KNLITVEVLK 

       190        200        210        220        230        240 
WSDGSYLEDQ DMWWFAGIYR DVYLYALPKF HIRDVFVRTD LDENYRNGKI FLDVEMRNLG 

       250        260        270        280        290        300 
EEEEKDLEVT LITPDGDEKT LVKETVKPED RVLSFAFDVK DPKKWSAETP HLYVLKLKLG 

       310        320        330        340        350        360 
EDEKKVNFGF RKIEIKDGTL LFNGKPLYIK GVNRHEFDPD RGHAVTVERM IQDIKLMKQH 

       370        380        390        400        410        420 
NINTVRTSHY PNQTKWYDLC DYFGLYVIDE ANIESHGIDW DPEVTLANRW EWEKAHFDRI 

       430        440        450        460        470        480 
KRMVERDKNH PSIIFWSLGN EAGDGVNFEK AALWIKKRDN TRLIHYEGTT RRGESYYVDV 

       490        500        510        520        530        540 
FSLMYPKMDI LLEYASKKRE KPFIMCEYAH AMGNSVGNLK DYWDVIEKYP YLHGGCIWDW 

       550        560        570        580        590        600 
VDQGIRKKDE NGREFWAYGG DFGDTPNDGN FCINGVVLPD RTPEPELYEV KKVYQNVKIR 

       610        620        630        640        650        660 
QVSKDTYEVE NRYLFTNLEM FDGAWKIRKD GEVIEEKTFK IFAEPGEKRL LKIPLPEMDD 

       670        680        690        700        710        720 
SEYFLEISFS LSEDTPWAEK GHVVAWEQFL LKAPAFEKKS ISDGVSLRED GKHLTVEAKD 

       730        740        750        760        770        780 
TVYVFSKLTG LLEQILHRRK KILKSPVVPN FWRVPTDNDI GNRMPQRLAI WKRASKERKL 

       790        800        810        820        830        840 
FKMHWKKEEN RVSVHSVFQL PGNSWVYTTY TVFGNGDVLV DLSLIPAEDV PEIPRIGFQF 

       850        860        870        880        890        900 
TVPEEFGTVE WYGRGPHETY WDRKESGLFA RYRKAVGEMM HRYVRPQETG NRSDVRWFAL 

       910        920        930        940        950        960 
SDGETKLFVS GMPQIDFSVW PFSMEDLERV QHISELPERD FVTVNVDFRQ MGLGGDDSWG 

       970        980        990       1000       1010       1020 
AMPHLEYRLL PKPYRFSFRM RISEEIPSWR VLAAIPETLH VEMSSEDVIR EGDTLRVKFS 

      1030       1040       1050       1060       1070       1080 
LLNDTPLSKE KQVVLFVDGN EYSVRRVVIP PFKKEELVFK VEGLKKGEHL IHTNLNTRKT 


IYVR 

« Hide

References

« Hide 'large scale' references
[1]"Identification and sequencing of the Thermotoga maritima lacZ gene, part of a divergently transcribed operon."
Moore J.B., Markiewicz P., Miller J.H.
Gene 147:101-106(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima."
Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. expand/collapse author list , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M.
Nature 399:323-329(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[3]"Properties of an alpha-galactosidase, and structure of its gene galA, within an alpha- and beta-galactoside utilization gene cluster of the hyperthermophilic bacterium Thermotoga maritima."
Liebl W., Wagner B., Schellhase J.
Syst. Appl. Microbiol. 21:1-11(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 554-1084.
Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U08186 Genomic DNA. Translation: AAA50597.1.
AE000512 Genomic DNA. Translation: AAD36268.1. Different initiation.
AJ001072 Genomic DNA. Translation: CAA04513.1.
PIRF72283.
RefSeqNP_228998.1. NC_000853.1.
YP_007977549.1. NC_021214.1.

3D structure databases

ProteinModelPortalQ56307.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243274.TM1193.

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD36268; AAD36268; TM_1193.
GeneID898291.
KEGGtma:TM1193.
tmm:Tmari_1200.
PATRIC23937328. VBITheMar51294_1211.

Phylogenomic databases

eggNOGCOG3250.
KOK01190.
OMANPPFVPK.
OrthoDBEOG6XWV0T.

Enzyme and pathway databases

BioCycTMAR243274:GC6P-1223-MONOMER.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_THEMA
AccessionPrimary (citable) accession number: Q56307
Secondary accession number(s): O33834
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 30, 2000
Last modified: July 9, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries