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Protein

Beta-galactosidase

Gene

lacZ

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei441 – 4411Proton donorBy similarity
Active sitei507 – 5071NucleophileBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciTMAR243274:GC6P-1223-MONOMER.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
Ordered Locus Names:TM_1193
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000008183: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10841084Beta-galactosidasePRO_0000057678Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243274.TM1193.

Structurei

3D structure databases

ProteinModelPortaliQ56307.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Phylogenomic databases

eggNOGiCOG3250.
InParanoidiQ56307.
KOiK01190.
OMAiWPFAQAD.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q56307-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYEWENPQL VSEGTEKSHA SFIPYLDPFS GEWEYPEEFI SLNGNWRFLF
60 70 80 90 100
AKNPFEVPED FFSEKFDDSN WDEIEVPSNW EMKGYGKPIY TNVVYPFEPN
110 120 130 140 150
PPFVPKDDNP TGVYRRWIEI PEDWFKKEIF LHFEGVRSFF YLWVNGKKIG
160 170 180 190 200
FSKDSCTPAE FRLTDVLRPG KNLITVEVLK WSDGSYLEDQ DMWWFAGIYR
210 220 230 240 250
DVYLYALPKF HIRDVFVRTD LDENYRNGKI FLDVEMRNLG EEEEKDLEVT
260 270 280 290 300
LITPDGDEKT LVKETVKPED RVLSFAFDVK DPKKWSAETP HLYVLKLKLG
310 320 330 340 350
EDEKKVNFGF RKIEIKDGTL LFNGKPLYIK GVNRHEFDPD RGHAVTVERM
360 370 380 390 400
IQDIKLMKQH NINTVRTSHY PNQTKWYDLC DYFGLYVIDE ANIESHGIDW
410 420 430 440 450
DPEVTLANRW EWEKAHFDRI KRMVERDKNH PSIIFWSLGN EAGDGVNFEK
460 470 480 490 500
AALWIKKRDN TRLIHYEGTT RRGESYYVDV FSLMYPKMDI LLEYASKKRE
510 520 530 540 550
KPFIMCEYAH AMGNSVGNLK DYWDVIEKYP YLHGGCIWDW VDQGIRKKDE
560 570 580 590 600
NGREFWAYGG DFGDTPNDGN FCINGVVLPD RTPEPELYEV KKVYQNVKIR
610 620 630 640 650
QVSKDTYEVE NRYLFTNLEM FDGAWKIRKD GEVIEEKTFK IFAEPGEKRL
660 670 680 690 700
LKIPLPEMDD SEYFLEISFS LSEDTPWAEK GHVVAWEQFL LKAPAFEKKS
710 720 730 740 750
ISDGVSLRED GKHLTVEAKD TVYVFSKLTG LLEQILHRRK KILKSPVVPN
760 770 780 790 800
FWRVPTDNDI GNRMPQRLAI WKRASKERKL FKMHWKKEEN RVSVHSVFQL
810 820 830 840 850
PGNSWVYTTY TVFGNGDVLV DLSLIPAEDV PEIPRIGFQF TVPEEFGTVE
860 870 880 890 900
WYGRGPHETY WDRKESGLFA RYRKAVGEMM HRYVRPQETG NRSDVRWFAL
910 920 930 940 950
SDGETKLFVS GMPQIDFSVW PFSMEDLERV QHISELPERD FVTVNVDFRQ
960 970 980 990 1000
MGLGGDDSWG AMPHLEYRLL PKPYRFSFRM RISEEIPSWR VLAAIPETLH
1010 1020 1030 1040 1050
VEMSSEDVIR EGDTLRVKFS LLNDTPLSKE KQVVLFVDGN EYSVRRVVIP
1060 1070 1080
PFKKEELVFK VEGLKKGEHL IHTNLNTRKT IYVR
Length:1,084
Mass (Da):127,608
Last modified:May 30, 2000 - v2
Checksum:iD52E3B762B53DDFC
GO

Sequence cautioni

The sequence AAD36268.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti152 – 17524SKDSC…KNLIT → RQRQLHARRIQTHRCSKTRE ESDH in AAA50597 (PubMed:8088532).CuratedAdd
BLAST
Sequence conflicti1028 – 108457SKEKQ…TIYVR → RQGKTGGSLC (PubMed:8088532).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08186 Genomic DNA. Translation: AAA50597.1.
AE000512 Genomic DNA. Translation: AAD36268.1. Different initiation.
AJ001072 Genomic DNA. Translation: CAA04513.1.
PIRiF72283.
RefSeqiNP_228998.1. NC_000853.1.
YP_007977549.1. NC_021214.1.

Genome annotation databases

EnsemblBacteriaiAAD36268; AAD36268; TM_1193.
GeneIDi15494802.
898291.
KEGGitma:TM1193.
PATRICi23937328. VBITheMar51294_1211.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08186 Genomic DNA. Translation: AAA50597.1.
AE000512 Genomic DNA. Translation: AAD36268.1. Different initiation.
AJ001072 Genomic DNA. Translation: CAA04513.1.
PIRiF72283.
RefSeqiNP_228998.1. NC_000853.1.
YP_007977549.1. NC_021214.1.

3D structure databases

ProteinModelPortaliQ56307.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM1193.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36268; AAD36268; TM_1193.
GeneIDi15494802.
898291.
KEGGitma:TM1193.
PATRICi23937328. VBITheMar51294_1211.

Phylogenomic databases

eggNOGiCOG3250.
InParanoidiQ56307.
KOiK01190.
OMAiWPFAQAD.
OrthoDBiEOG6XWV0T.

Enzyme and pathway databases

BioCyciTMAR243274:GC6P-1223-MONOMER.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and sequencing of the Thermotoga maritima lacZ gene, part of a divergently transcribed operon."
    Moore J.B., Markiewicz P., Miller J.H.
    Gene 147:101-106(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  3. "Properties of an alpha-galactosidase, and structure of its gene galA, within an alpha- and beta-galactoside utilization gene cluster of the hyperthermophilic bacterium Thermotoga maritima."
    Liebl W., Wagner B., Schellhase J.
    Syst. Appl. Microbiol. 21:1-11(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 554-1084.
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Entry informationi

Entry nameiBGAL_THEMA
AccessioniPrimary (citable) accession number: Q56307
Secondary accession number(s): O33834
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 30, 2000
Last modified: March 4, 2015
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.