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Q56307

- BGAL_THEMA

UniProt

Q56307 - BGAL_THEMA

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Protein

Beta-galactosidase

Gene

lacZ

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei441 – 4411Proton donorBy similarity
Active sitei507 – 5071NucleophileBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciTMAR243274:GC6P-1223-MONOMER.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
Ordered Locus Names:TM_1193
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000008183: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10841084Beta-galactosidasePRO_0000057678Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243274.TM1193.

Structurei

3D structure databases

ProteinModelPortaliQ56307.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Phylogenomic databases

eggNOGiCOG3250.
InParanoidiQ56307.
KOiK01190.
OMAiNPPFVPK.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q56307-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPYEWENPQL VSEGTEKSHA SFIPYLDPFS GEWEYPEEFI SLNGNWRFLF
60 70 80 90 100
AKNPFEVPED FFSEKFDDSN WDEIEVPSNW EMKGYGKPIY TNVVYPFEPN
110 120 130 140 150
PPFVPKDDNP TGVYRRWIEI PEDWFKKEIF LHFEGVRSFF YLWVNGKKIG
160 170 180 190 200
FSKDSCTPAE FRLTDVLRPG KNLITVEVLK WSDGSYLEDQ DMWWFAGIYR
210 220 230 240 250
DVYLYALPKF HIRDVFVRTD LDENYRNGKI FLDVEMRNLG EEEEKDLEVT
260 270 280 290 300
LITPDGDEKT LVKETVKPED RVLSFAFDVK DPKKWSAETP HLYVLKLKLG
310 320 330 340 350
EDEKKVNFGF RKIEIKDGTL LFNGKPLYIK GVNRHEFDPD RGHAVTVERM
360 370 380 390 400
IQDIKLMKQH NINTVRTSHY PNQTKWYDLC DYFGLYVIDE ANIESHGIDW
410 420 430 440 450
DPEVTLANRW EWEKAHFDRI KRMVERDKNH PSIIFWSLGN EAGDGVNFEK
460 470 480 490 500
AALWIKKRDN TRLIHYEGTT RRGESYYVDV FSLMYPKMDI LLEYASKKRE
510 520 530 540 550
KPFIMCEYAH AMGNSVGNLK DYWDVIEKYP YLHGGCIWDW VDQGIRKKDE
560 570 580 590 600
NGREFWAYGG DFGDTPNDGN FCINGVVLPD RTPEPELYEV KKVYQNVKIR
610 620 630 640 650
QVSKDTYEVE NRYLFTNLEM FDGAWKIRKD GEVIEEKTFK IFAEPGEKRL
660 670 680 690 700
LKIPLPEMDD SEYFLEISFS LSEDTPWAEK GHVVAWEQFL LKAPAFEKKS
710 720 730 740 750
ISDGVSLRED GKHLTVEAKD TVYVFSKLTG LLEQILHRRK KILKSPVVPN
760 770 780 790 800
FWRVPTDNDI GNRMPQRLAI WKRASKERKL FKMHWKKEEN RVSVHSVFQL
810 820 830 840 850
PGNSWVYTTY TVFGNGDVLV DLSLIPAEDV PEIPRIGFQF TVPEEFGTVE
860 870 880 890 900
WYGRGPHETY WDRKESGLFA RYRKAVGEMM HRYVRPQETG NRSDVRWFAL
910 920 930 940 950
SDGETKLFVS GMPQIDFSVW PFSMEDLERV QHISELPERD FVTVNVDFRQ
960 970 980 990 1000
MGLGGDDSWG AMPHLEYRLL PKPYRFSFRM RISEEIPSWR VLAAIPETLH
1010 1020 1030 1040 1050
VEMSSEDVIR EGDTLRVKFS LLNDTPLSKE KQVVLFVDGN EYSVRRVVIP
1060 1070 1080
PFKKEELVFK VEGLKKGEHL IHTNLNTRKT IYVR
Length:1,084
Mass (Da):127,608
Last modified:May 30, 2000 - v2
Checksum:iD52E3B762B53DDFC
GO

Sequence cautioni

The sequence AAD36268.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti152 – 17524SKDSC…KNLIT → RQRQLHARRIQTHRCSKTRE ESDH in AAA50597. (PubMed:8088532)CuratedAdd
BLAST
Sequence conflicti1028 – 108457SKEKQ…TIYVR → RQGKTGGSLC(PubMed:8088532)CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U08186 Genomic DNA. Translation: AAA50597.1.
AE000512 Genomic DNA. Translation: AAD36268.1. Different initiation.
AJ001072 Genomic DNA. Translation: CAA04513.1.
PIRiF72283.
RefSeqiNP_228998.1. NC_000853.1.
YP_007977549.1. NC_021214.1.

Genome annotation databases

EnsemblBacteriaiAAD36268; AAD36268; TM_1193.
GeneIDi898291.
KEGGitma:TM1193.
tmm:Tmari_1200.
PATRICi23937328. VBITheMar51294_1211.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U08186 Genomic DNA. Translation: AAA50597.1 .
AE000512 Genomic DNA. Translation: AAD36268.1 . Different initiation.
AJ001072 Genomic DNA. Translation: CAA04513.1 .
PIRi F72283.
RefSeqi NP_228998.1. NC_000853.1.
YP_007977549.1. NC_021214.1.

3D structure databases

ProteinModelPortali Q56307.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243274.TM1193.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD36268 ; AAD36268 ; TM_1193 .
GeneIDi 898291.
KEGGi tma:TM1193.
tmm:Tmari_1200.
PATRICi 23937328. VBITheMar51294_1211.

Phylogenomic databases

eggNOGi COG3250.
InParanoidi Q56307.
KOi K01190.
OMAi NPPFVPK.
OrthoDBi EOG6XWV0T.

Enzyme and pathway databases

BioCyci TMAR243274:GC6P-1223-MONOMER.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and sequencing of the Thermotoga maritima lacZ gene, part of a divergently transcribed operon."
    Moore J.B., Markiewicz P., Miller J.H.
    Gene 147:101-106(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  3. "Properties of an alpha-galactosidase, and structure of its gene galA, within an alpha- and beta-galactoside utilization gene cluster of the hyperthermophilic bacterium Thermotoga maritima."
    Liebl W., Wagner B., Schellhase J.
    Syst. Appl. Microbiol. 21:1-11(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 554-1084.
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Entry informationi

Entry nameiBGAL_THEMA
AccessioniPrimary (citable) accession number: Q56307
Secondary accession number(s): O33834
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 30, 2000
Last modified: October 29, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3