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Protein

Beta-galactosidase

Gene

lacZ

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei441Proton donorBy similarity1
Active sitei507NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Protein family/group databases

CAZyiGH2 Glycoside Hydrolase Family 2

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
Ordered Locus Names:TM_1193
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000576781 – 1084Beta-galactosidaseAdd BLAST1084

Proteomic databases

PRIDEiQ56307

Interactioni

Protein-protein interaction databases

STRINGi243274.TM1193

Structurei

3D structure databases

ProteinModelPortaliQ56307
SMRiQ56307
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Phylogenomic databases

eggNOGiENOG4105CNT Bacteria
COG3250 LUCA
InParanoidiQ56307
KOiK01190
OMAiPSNWQLQ

Family and domain databases

Gene3Di2.60.120.260, 1 hit
2.60.40.10, 2 hits
2.70.98.10, 1 hit
InterProiView protein in InterPro
IPR004199 B-gal_small/dom_5
IPR036156 Beta-gal/glucu_dom_sf
IPR011013 Gal_mutarotase_sf_dom
IPR008979 Galactose-bd-like_sf
IPR014718 GH-type_carb-bd
IPR006101 Glyco_hydro_2
IPR023232 Glyco_hydro_2_AS
IPR006103 Glyco_hydro_2_cat
IPR023230 Glyco_hydro_2_CS
IPR006102 Glyco_hydro_2_Ig-like
IPR006104 Glyco_hydro_2_N
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR032312 LacZ_4
PfamiView protein in Pfam
PF02929 Bgal_small_N, 1 hit
PF16353 DUF4981, 1 hit
PF00703 Glyco_hydro_2, 1 hit
PF02836 Glyco_hydro_2_C, 1 hit
PF02837 Glyco_hydro_2_N, 1 hit
PRINTSiPR00132 GLHYDRLASE2
SMARTiView protein in SMART
SM01038 Bgal_small_N, 1 hit
SUPFAMiSSF49303 SSF49303, 2 hits
SSF49785 SSF49785, 1 hit
SSF51445 SSF51445, 1 hit
SSF74650 SSF74650, 1 hit
PROSITEiView protein in PROSITE
PS00719 GLYCOSYL_HYDROL_F2_1, 1 hit
PS00608 GLYCOSYL_HYDROL_F2_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q56307-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYEWENPQL VSEGTEKSHA SFIPYLDPFS GEWEYPEEFI SLNGNWRFLF
60 70 80 90 100
AKNPFEVPED FFSEKFDDSN WDEIEVPSNW EMKGYGKPIY TNVVYPFEPN
110 120 130 140 150
PPFVPKDDNP TGVYRRWIEI PEDWFKKEIF LHFEGVRSFF YLWVNGKKIG
160 170 180 190 200
FSKDSCTPAE FRLTDVLRPG KNLITVEVLK WSDGSYLEDQ DMWWFAGIYR
210 220 230 240 250
DVYLYALPKF HIRDVFVRTD LDENYRNGKI FLDVEMRNLG EEEEKDLEVT
260 270 280 290 300
LITPDGDEKT LVKETVKPED RVLSFAFDVK DPKKWSAETP HLYVLKLKLG
310 320 330 340 350
EDEKKVNFGF RKIEIKDGTL LFNGKPLYIK GVNRHEFDPD RGHAVTVERM
360 370 380 390 400
IQDIKLMKQH NINTVRTSHY PNQTKWYDLC DYFGLYVIDE ANIESHGIDW
410 420 430 440 450
DPEVTLANRW EWEKAHFDRI KRMVERDKNH PSIIFWSLGN EAGDGVNFEK
460 470 480 490 500
AALWIKKRDN TRLIHYEGTT RRGESYYVDV FSLMYPKMDI LLEYASKKRE
510 520 530 540 550
KPFIMCEYAH AMGNSVGNLK DYWDVIEKYP YLHGGCIWDW VDQGIRKKDE
560 570 580 590 600
NGREFWAYGG DFGDTPNDGN FCINGVVLPD RTPEPELYEV KKVYQNVKIR
610 620 630 640 650
QVSKDTYEVE NRYLFTNLEM FDGAWKIRKD GEVIEEKTFK IFAEPGEKRL
660 670 680 690 700
LKIPLPEMDD SEYFLEISFS LSEDTPWAEK GHVVAWEQFL LKAPAFEKKS
710 720 730 740 750
ISDGVSLRED GKHLTVEAKD TVYVFSKLTG LLEQILHRRK KILKSPVVPN
760 770 780 790 800
FWRVPTDNDI GNRMPQRLAI WKRASKERKL FKMHWKKEEN RVSVHSVFQL
810 820 830 840 850
PGNSWVYTTY TVFGNGDVLV DLSLIPAEDV PEIPRIGFQF TVPEEFGTVE
860 870 880 890 900
WYGRGPHETY WDRKESGLFA RYRKAVGEMM HRYVRPQETG NRSDVRWFAL
910 920 930 940 950
SDGETKLFVS GMPQIDFSVW PFSMEDLERV QHISELPERD FVTVNVDFRQ
960 970 980 990 1000
MGLGGDDSWG AMPHLEYRLL PKPYRFSFRM RISEEIPSWR VLAAIPETLH
1010 1020 1030 1040 1050
VEMSSEDVIR EGDTLRVKFS LLNDTPLSKE KQVVLFVDGN EYSVRRVVIP
1060 1070 1080
PFKKEELVFK VEGLKKGEHL IHTNLNTRKT IYVR
Length:1,084
Mass (Da):127,608
Last modified:May 30, 2000 - v2
Checksum:iD52E3B762B53DDFC
GO

Sequence cautioni

The sequence AAD36268 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti152 – 175SKDSC…KNLIT → RQRQLHARRIQTHRCSKTRE ESDH in AAA50597 (PubMed:8088532).CuratedAdd BLAST24
Sequence conflicti1028 – 1084SKEKQ…TIYVR → RQGKTGGSLC (PubMed:8088532).CuratedAdd BLAST57

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08186 Genomic DNA Translation: AAA50597.1
AE000512 Genomic DNA Translation: AAD36268.1 Different initiation.
AJ001072 Genomic DNA Translation: CAA04513.1
PIRiF72283
RefSeqiNP_228998.1, NC_000853.1

Genome annotation databases

EnsemblBacteriaiAAD36268; AAD36268; TM_1193
GeneIDi898291
KEGGitma:TM1193
PATRICifig|243274.5.peg.1211

Similar proteinsi

Entry informationi

Entry nameiBGAL_THEMA
AccessioniPrimary (citable) accession number: Q56307
Secondary accession number(s): O33834
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 30, 2000
Last modified: May 23, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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