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Protein

Glutamate dehydrogenase

Gene

gdhA

Organism
Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.PROSITE-ProRule annotation

pH dependencei

Optimum pH is 8.0.

Temperature dependencei

Optimum temperature is above 95 degrees Celsius.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei105PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi219 – 225NADSequence analysis7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD, NADP

Enzyme and pathway databases

BRENDAi1.4.1.3. 6302.
1.4.1.4. 6302.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate dehydrogenase (EC:1.4.1.3)
Short name:
GDH
Gene namesi
Name:gdhA
ORF Names:OCC_00135
OrganismiThermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C)
Taxonomic identifieri523849 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000015502 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001827622 – 419Glutamate dehydrogenaseAdd BLAST418

Interactioni

Subunit structurei

Homohexamer.1 Publication

Structurei

Secondary structure

1419
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 18Combined sources14
Helixi25 – 30Combined sources6
Beta strandi35 – 45Combined sources11
Beta strandi51 – 62Combined sources12
Beta strandi66 – 69Combined sources4
Beta strandi72 – 75Combined sources4
Helixi80 – 97Combined sources18
Beta strandi102 – 110Combined sources9
Helixi112 – 114Combined sources3
Helixi117 – 131Combined sources15
Helixi132 – 134Combined sources3
Turni137 – 139Combined sources3
Helixi150 – 164Combined sources15
Helixi170 – 173Combined sources4
Beta strandi174 – 176Combined sources3
Helixi179 – 181Combined sources3
Helixi190 – 205Combined sources16
Beta strandi214 – 218Combined sources5
Helixi222 – 235Combined sources14
Beta strandi238 – 243Combined sources6
Beta strandi248 – 250Combined sources3
Helixi257 – 267Combined sources11
Beta strandi268 – 270Combined sources3
Beta strandi277 – 279Combined sources3
Helixi281 – 286Combined sources6
Beta strandi290 – 294Combined sources5
Helixi303 – 306Combined sources4
Beta strandi312 – 315Combined sources4
Beta strandi318 – 320Combined sources3
Helixi324 – 333Combined sources10
Beta strandi336 – 338Combined sources3
Helixi340 – 343Combined sources4
Helixi346 – 360Combined sources15
Helixi366 – 391Combined sources26
Helixi395 – 414Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BVUX-ray2.50A/B/C/D/E/F2-419[»]
SMRiQ56304.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ56304.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK00261.

Family and domain databases

CDDicd01076. NAD_bind_1_Glu_DH. 1 hit.
Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR033524. Glu/Leu/Phe/Val_DH_AS.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
IPR033922. NAD_bind_Glu_DH.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q56304-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEQDPFEIA VKQLERAAQY MDISEEALEF LKRPQRIVEV SIPVEMDDGS
60 70 80 90 100
VKVFTGFRVQ YNWARGPTKG GIRWHPEETL STVKALAAWM TWKTAVMDLP
110 120 130 140 150
YGGGKGGVIC NPKEMSDREK ERLARGYVRA IYDVISPYTD IPAPDVYTNP
160 170 180 190 200
QIMAWMMDEY ETISRRKDPS FGVITGKPPS VGGIVARMDA TARGASYTVR
210 220 230 240 250
EAAKALGMDL KGKTIAIQGY GNAGYYMAKI MSEEYGMKVV AVSDSKGGIY
260 270 280 290 300
NPDGLNADEV LAWKKKTGSV KDFPGATNIT NEELLELEVD VLAPSAIEEV
310 320 330 340 350
ITKKNADNIK AKIVAELANG PTTPEADEIL YEKGILIIPD FLCNAGGVTV
360 370 380 390 400
SYFEWVQNIT GDYWTVEETR AKLDKKMTKA FWDVYNTHKE KNINMRDAAY
410
VVAVSRVYQA MKDRGWIKK
Length:419
Mass (Da):46,726
Last modified:May 14, 2014 - v4
Checksum:iF53A237F0C5B7215
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti245S → T in AAA72393 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19995 Genomic DNA. Translation: AAA72393.1.
CP006670 Genomic DNA. Translation: EHR77478.1.
RefSeqiWP_004070133.1. NC_022084.1.

Genome annotation databases

EnsemblBacteriaiEHR77478; EHR77478; OCC_00135.
GeneIDi16550867.
KEGGitlt:OCC_00135.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19995 Genomic DNA. Translation: AAA72393.1.
CP006670 Genomic DNA. Translation: EHR77478.1.
RefSeqiWP_004070133.1. NC_022084.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BVUX-ray2.50A/B/C/D/E/F2-419[»]
SMRiQ56304.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiEHR77478; EHR77478; OCC_00135.
GeneIDi16550867.
KEGGitlt:OCC_00135.

Phylogenomic databases

KOiK00261.

Enzyme and pathway databases

BRENDAi1.4.1.3. 6302.
1.4.1.4. 6302.

Miscellaneous databases

EvolutionaryTraceiQ56304.

Family and domain databases

CDDicd01076. NAD_bind_1_Glu_DH. 1 hit.
Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR033524. Glu/Leu/Phe/Val_DH_AS.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
IPR033922. NAD_bind_Glu_DH.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHE3_THELN
AccessioniPrimary (citable) accession number: Q56304
Secondary accession number(s): H3ZRF0, Q9UWK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 14, 2014
Last modified: November 30, 2016
This is version 95 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.