Reviewed, UniProtKB/Swiss-Prot Q56303 (FOR_THELI)

Names and origin

Protein names

Recommended name:
    Tungsten-containing formaldehyde ferredoxin oxidoreductase
    EC=1.2.7.5

Gene names

Name:

for

Organism

Thermococcus litoralis

Taxonomic identifier

2265 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus

Protein attributes

Sequence length	621 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the oxidation of C1-C3 aldehydes. Involved in peptides fermentation.
Catalytic activity	An aldehyde + H2O + 2 oxidized ferredoxin = an acid + 2 H+ + 2 reduced ferredoxin.
Cofactor	Binds 1 4Fe-4S cluster per subunit. Ref.2 Binds 1 tungstopterin cofactor per subunit. Ref.2
Enzyme regulation	Inhibited by arsenite, iodoacetate and cyanide.
Subunit structure	Homotetramer. Ref.2
Miscellaneous	A low amount of aldehyde ferredoxin oxidoreductase activity has been observed.
Sequence similarities	Belongs to the AOR/FOR family.
biophysicochemical properties	Kinetic parameters: Significant activity only with aliphatic aldehydes up to C3 and only at high concentration (15 mM). KM=62 mM for formaldehyde Vmax=17.5 µmol/min/mg enzyme with formaldehyde as substrate pH dependence: Optimum pH is above 10 (at 80 degrees Celsius). Temperature dependence: Optimum temperature is above 90 degrees Celsius (at pH 8.4).

Ontologies

Keywords
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Tungsten
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW aldehyde ferredoxin oxidoreductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 621	621	Tungsten-containing formaldehyde ferredoxin oxidoreductase		PRO_0000087325
Sites
	Metal binding	284	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	287	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	291	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	493	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q56303-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 95589C2DC4F18E13	FASTA	621	68,941
	10 20 30 40 50 60 MKGWWGKILR VDLTNNKVWV QEYSPEVAKN FIGGRGLAAW ILWNEAKNVD PLGPKNKLVF 70 80 90 100 110 120 ATGPFNGLPT PSGGKMVIAA KSPLTGGYGD GNLGTMATVH LRKAGYDALV VEGKAKKPVY 130 140 150 160 170 180 IYIEDDNVSI LSAEGLWGKT TFETERELKE IHGKNVGILS IGPGGENLVK YAVVISQEGR 190 200 210 220 230 240 AAGRPGMGAV MGSKKLKAVV IKGTKEIPVA DKEKLKELSQ EAYDAILNSP GYPFWHRQGT 250 260 270 280 290 300 MAAVEWTNEN SALPTRNFSD GSFEFARSID GYTMEGMKVK QRGCPYCNMP CGNVVLDAEG 310 320 330 340 350 360 QESELDYENV ALLGSNLGIG KLNEVSVLNR IADEMGLDTI SLGVAISYVM EAKEKGIIKD 370 380 390 400 410 420 DDAPEFGDFK KAKQLALDIA YRRGELGNLA AEGVKVMSEK LGAKDFAMHV KGLEVSGYNC 430 440 450 460 470 480 YIYPAMALAY GTSSIGAHHK EAWVIAWEIG TAPIEGEKAQ KVEYKITYDP EKAAKVIELQ 490 500 510 520 530 540 RLRGGLFEML TACRLPWVEV GLSLDYYPKL LEAITGVKYT WDDLYKAADR VYALMRAYWV 550 560 570 580 590 600 REFNGNWSRE MDYPPERWFK EGLKSGPYKG QHLEKDKYDA LLSEYYKLRG WDERGIPKKE 610 620 TLKELNLEFV IPELEKVTKL E

References

[1]

"Molecular characterization of the genes encoding the tungsten-containing aldehyde ferredoxin oxidoreductase from Pyrococcus furiosus and formaldehyde ferredoxin oxidoreductase from Thermococcus litoralis." Kletzin A., Mukund S., Kelley-Crouse T.L., Chan M.K.S., Rees D.C., Adams M.W.W. J. Bacteriol. 177:4817-4819(1995) [PubMed: 7642512] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 5473.

[2]

"Characterization of a novel tungsten-containing formaldehyde ferredoxin oxidoreductase from the hyperthermophilic archaeon, Thermococcus litoralis. A role for tungsten in peptide catabolism." Mukund S., Adams M.W.W. J. Biol. Chem. 268:13592-13600(1993) [PubMed: 8390467] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-26, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, CHARACTERIZATION. Strain: DSM 5473.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X83963 Genomic DNA. Translation: CAA58795.1.
3D structure databases
HSSP	HSSP built from PDB template 1B25 based on UniProtKB O93738.
SMR	Q56303. Positions 1-621.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.2.7.5. 74708.
Family and domain databases
InterPro	IPR013985. Ald_Fedxn_OxRdtase_3. IPR013983. Ald_Fedxn_OxRdtase_N. IPR001203. OxRdtase_Ald_Fedxn_C. [Graphical view]
Gene3D	G3DSA:1.10.599.10. Oxred_Ald_Fedxn_3. 1 hit. G3DSA:3.60.9.10. Oxred_Ald_Fedxn_N. 1 hit.
Pfam	PF01314. AFOR_C. 1 hit. PF02730. AFOR_N. 1 hit. [Graphical view]
SMART	SM00790. AFOR_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FOR_THELI

Accession

Primary (citable) accession number: Q56303

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	January 20, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)