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Q56301 (KPYK_THELN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase

Short name=PK
EC=2.7.1.40
Gene names
Name:pki
ORF Names:OCC_05009
OrganismThermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C) [Complete proteome] [HAMAP]
Taxonomic identifier523849 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

Protein attributes

Sequence length475 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Divalent metal cations By similarity.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the pyruvate kinase family.

Sequence caution

The sequence CAA58793.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 475475Pyruvate kinase
PRO_0000112130

Sites

Metal binding381Potassium By similarity
Metal binding401Potassium By similarity
Metal binding701Potassium By similarity
Metal binding2231Magnesium By similarity
Metal binding2471Magnesium By similarity
Binding site361Substrate By similarity
Binding site2461Substrate; via amide nitrogen By similarity
Binding site2471Substrate; via amide nitrogen By similarity
Binding site2791Substrate By similarity
Site2211Transition state stabilizer By similarity

Experimental info

Sequence conflict231T → S in CAA58793. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q56301 [UniParc].

Last modified May 14, 2014. Version 2.
Checksum: 7970729B9948B94A

FASTA47552,767
        10         20         30         40         50         60 
MELPSHKTKI IATIGPASKQ KETIKKMIKA GMSVARINFS HGTLEEHAKT IETVRDVAEK 

        70         80         90        100        110        120 
LERRVAILGD LPGLKMRVGK IKGDSVTLRK GDKVVLTTRD IEGDETTIPV EFKDLPKLVS 

       130        140        150        160        170        180 
KGDTIYLSDG YIMLRVEEVR ENEVECVVVN GGILFSHKGI NIPKANLPIE AITPRDFEII 

       190        200        210        220        230        240 
EFAIEHGVDA IGLSFVGSVY DVLKVKSFLE KKSADLFVIA KIERPDAVRN FDEILNAADG 

       250        260        270        280        290        300 
IMIARGDLGV EMPIEKLPIM QKQLIKKTNL AAKPVITATQ MLVSMTTERI PKRAEVTDVA 

       310        320        330        340        350        360 
NAILDGTDAV MLSEETAIGK YPVESVEMMA KIAKTTEEYR ESLGYSRLRA WIDSLPKRST 

       370        380        390        400        410        420 
IKEAITRSVI DALCAIDIKY ILTPTRTGLT PRLISRFKPK QWILAFSSSE RVCNNLAFSY 

       430        440        450        460        470 
GVYPFCMDEN FNEKDIIMLV KGLGIVKEDD TVLLTEGRPI GKTVGTNTMR IFQIP 

« Hide

References

« Hide 'large scale' references
[1]"Genome sequence of the model hyperthermophilic archaeon Thermococcus litoralis NS-C."
Gardner A.F., Kumar S., Perler F.B.
J. Bacteriol. 194:2375-2376(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51850 / DSM 5473 / JCM 8560 / NS-C.
[2]"Molecular characterization of the genes encoding the tungsten-containing aldehyde ferredoxin oxidoreductase from Pyrococcus furiosus and formaldehyde ferredoxin oxidoreductase from Thermococcus litoralis."
Kletzin A., Mukund S., Kelley-Crouse T.L., Chan M.K.S., Rees D.C., Adams M.W.W.
J. Bacteriol. 177:4817-4819(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-216.
Strain: ATCC 51850 / DSM 5473 / JCM 8560 / NS-C.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP006670 Genomic DNA. Translation: EHR79867.1.
X83963 Genomic DNA. Translation: CAA58793.1. Different initiation.
PIRA57418.
RefSeqYP_008428436.1. NC_022084.1.

3D structure databases

ProteinModelPortalQ56301.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID16548766.
KEGGtlt:OCC_05009.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK00873.

Enzyme and pathway databases

UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPYK_THELN
AccessionPrimary (citable) accession number: Q56301
Secondary accession number(s): H3ZJK4
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 14, 2014
Last modified: June 11, 2014
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways