Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q56301

- KPYK_THELN

UniProt

Q56301 - KPYK_THELN

Protein

Pyruvate kinase

Gene

pki

Organism
Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 2 (14 May 2014)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Divalent metal cations.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei36 – 361SubstrateBy similarity
    Metal bindingi38 – 381PotassiumBy similarity
    Metal bindingi40 – 401PotassiumBy similarity
    Metal bindingi70 – 701PotassiumBy similarity
    Sitei221 – 2211Transition state stabilizerBy similarity
    Metal bindingi223 – 2231MagnesiumBy similarity
    Binding sitei246 – 2461Substrate; via amide nitrogenBy similarity
    Metal bindingi247 – 2471MagnesiumBy similarity
    Binding sitei247 – 2471Substrate; via amide nitrogenBy similarity
    Binding sitei279 – 2791SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase (EC:2.7.1.40)
    Short name:
    PK
    Gene namesi
    Name:pki
    ORF Names:OCC_05009
    OrganismiThermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C)
    Taxonomic identifieri523849 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
    ProteomesiUP000015502: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 475475Pyruvate kinasePRO_0000112130Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ56301.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q56301-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELPSHKTKI IATIGPASKQ KETIKKMIKA GMSVARINFS HGTLEEHAKT    50
    IETVRDVAEK LERRVAILGD LPGLKMRVGK IKGDSVTLRK GDKVVLTTRD 100
    IEGDETTIPV EFKDLPKLVS KGDTIYLSDG YIMLRVEEVR ENEVECVVVN 150
    GGILFSHKGI NIPKANLPIE AITPRDFEII EFAIEHGVDA IGLSFVGSVY 200
    DVLKVKSFLE KKSADLFVIA KIERPDAVRN FDEILNAADG IMIARGDLGV 250
    EMPIEKLPIM QKQLIKKTNL AAKPVITATQ MLVSMTTERI PKRAEVTDVA 300
    NAILDGTDAV MLSEETAIGK YPVESVEMMA KIAKTTEEYR ESLGYSRLRA 350
    WIDSLPKRST IKEAITRSVI DALCAIDIKY ILTPTRTGLT PRLISRFKPK 400
    QWILAFSSSE RVCNNLAFSY GVYPFCMDEN FNEKDIIMLV KGLGIVKEDD 450
    TVLLTEGRPI GKTVGTNTMR IFQIP 475
    Length:475
    Mass (Da):52,767
    Last modified:May 14, 2014 - v2
    Checksum:i7970729B9948B94A
    GO

    Sequence cautioni

    The sequence CAA58793.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti23 – 231T → S in CAA58793. (PubMed:7642512)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP006670 Genomic DNA. Translation: EHR79867.1.
    X83963 Genomic DNA. Translation: CAA58793.1. Different initiation.
    PIRiA57418.
    RefSeqiWP_004066098.1. NC_022084.1.
    YP_008428436.1. NC_022084.1.

    Genome annotation databases

    GeneIDi16548766.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP006670 Genomic DNA. Translation: EHR79867.1 .
    X83963 Genomic DNA. Translation: CAA58793.1 . Different initiation.
    PIRi A57418.
    RefSeqi WP_004066098.1. NC_022084.1.
    YP_008428436.1. NC_022084.1.

    3D structure databases

    ProteinModelPortali Q56301.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 16548766.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the model hyperthermophilic archaeon Thermococcus litoralis NS-C."
      Gardner A.F., Kumar S., Perler F.B.
      J. Bacteriol. 194:2375-2376(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51850 / DSM 5473 / JCM 8560 / NS-C.
    2. "Molecular characterization of the genes encoding the tungsten-containing aldehyde ferredoxin oxidoreductase from Pyrococcus furiosus and formaldehyde ferredoxin oxidoreductase from Thermococcus litoralis."
      Kletzin A., Mukund S., Kelley-Crouse T.L., Chan M.K.S., Rees D.C., Adams M.W.W.
      J. Bacteriol. 177:4817-4819(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-216.
      Strain: ATCC 51850 / DSM 5473 / JCM 8560 / NS-C.

    Entry informationi

    Entry nameiKPYK_THELN
    AccessioniPrimary (citable) accession number: Q56301
    Secondary accession number(s): H3ZJK4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: May 14, 2014
    Last modified: October 1, 2014
    This is version 70 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3