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Protein

Nef-associated protein 1

Gene

Nap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes acyl-CoA thioesters (in vitro). Has a preference for substrates with medium chain length (C10-C14). Inactive towards substrates with C18 or C20 aliphatic chains. Its physiological substrate is not known (By similarity).By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Nef-associated protein 1 (EC:3.1.2.-)
Alternative name(s):
Thioesterase NAP1
Gene namesi
Name:Nap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1922003. 5830415F09Rik.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 431431Nef-associated protein 1PRO_0000288887Add
BLAST

Proteomic databases

PRIDEiQ562D6.

PTM databases

PhosphoSiteiQ562D6.

Expressioni

Gene expression databases

BgeeiQ562D6.
CleanExiMM_5830415F09RIK.
ExpressionAtlasiQ562D6. baseline and differential.
GenevisibleiQ562D6. MM.

Interactioni

Subunit structurei

Interacts with lentivirus protein Nef.By similarity

Protein-protein interaction databases

BioGridi216996. 1 interaction.
IntActiQ562D6. 1 interaction.
MINTiMINT-8414996.

Structurei

3D structure databases

ProteinModelPortaliQ562D6.
SMRiQ562D6. Positions 27-221.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 168139TsaA-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 TsaA-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1720.
GeneTreeiENSGT00390000004643.
HOGENOMiHOG000231645.
HOVERGENiHBG054288.
InParanoidiQ562D6.
OMAiYEIACAP.
TreeFamiTF331670.

Family and domain databases

Gene3Di2.40.30.70. 1 hit.
InterProiIPR023370. TsaA-like.
IPR001378. UPF0066.
IPR023368. UPF0066_cons_site.
[Graphical view]
PfamiPF01980. UPF0066. 1 hit.
[Graphical view]
SUPFAMiSSF118196. SSF118196. 2 hits.
TIGRFAMsiTIGR00104. tRNA_TsaA. 1 hit.
PROSITEiPS01318. TSAA_1. 1 hit.
PS51668. TSAA_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q562D6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRGLEKQGSC ATAAPCGCVQ PALEAGNLLT EPIGYLESCF PAKIGTPRQP
60 70 80 90 100
SICSHSRACL KIRKNIFNNP EHSLMGLEEF SHVWILFVFH KNGHLNYKAK
110 120 130 140 150
VQPPRLNGAK TGVFSTRSPH RPNAIGLTLA KLEKVEGGAV YLSGVDMIDG
160 170 180 190 200
TPVLDIKPYI ADYDSPQNLS VHNDHHKLRA EAQVDGTANS CDQLLLSGRG
210 220 230 240 250
KVQPRQSTKE RPKCLEDRTS GENSQKSRDM SEIQHTLPED RERALDLALE
260 270 280 290 300
PSRGESMDMP ENQLGPPELK SFLEEGTDRP RKVEGALVLP GSSAETQWDA
310 320 330 340 350
SYRARTADRV PYSVVPSWVT EAPVAPLQVR FTPHAEMDLR KLNSGDASQP
360 370 380 390 400
SFKYFHSAEE AKRAIEAVLS ADPRSVYRRK LCEDRLFFFT VDTAHVTCWF
410 420 430
GDGFAEVVRI KLASESVQVA DPEESLAALG S
Length:431
Mass (Da):47,559
Last modified:May 29, 2007 - v2
Checksum:i5C98C96BE6A6692B
GO
Isoform 2 (identifier: Q562D6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     419-431: VADPEESLAALGS → TVLPPSPSPN...PPLPQGLVFS

Note: No experimental confirmation available.
Show »
Length:488
Mass (Da):53,677
Checksum:iA20D1CD4D29888AE
GO
Isoform 3 (identifier: Q562D6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-25: MRGLEKQGSCATAAPCGCVQPALEA → MWVRSAGPG

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.
Show »
Length:415
Mass (Da):46,027
Checksum:i893560AF2A75659B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti22 – 221A → V in AAH92543 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2525MRGLE…PALEA → MWVRSAGPG in isoform 3. 1 PublicationVSP_025815Add
BLAST
Alternative sequencei419 – 43113VADPE…AALGS → TVLPPSPSPNGREEQSSVEE EVQQRAGPSRRAAPRAASML LTPQSSSLATSALTHWAISL PPLPQGLVFS in isoform 2. 1 PublicationVSP_025816Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017931 mRNA. Translation: BAB31009.1.
AK044073 mRNA. Translation: BAC31763.1.
AK153576 mRNA. Translation: BAE32105.1.
AL806523 Genomic DNA. Translation: CAM23957.1.
BC092543 mRNA. Translation: AAH92543.1.
CCDSiCCDS18148.1. [Q562D6-2]
RefSeqiNP_083362.1. NM_029086.2. [Q562D6-2]
XP_006538388.1. XM_006538325.2. [Q562D6-1]
UniGeneiMm.45973.

Genome annotation databases

EnsembliENSMUST00000030015; ENSMUSP00000030015; ENSMUSG00000028331. [Q562D6-1]
ENSMUST00000086563; ENSMUSP00000083752; ENSMUSG00000028331. [Q562D6-2]
GeneIDi74753.
KEGGimmu:74753.
UCSCiuc008stq.1. mouse. [Q562D6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017931 mRNA. Translation: BAB31009.1.
AK044073 mRNA. Translation: BAC31763.1.
AK153576 mRNA. Translation: BAE32105.1.
AL806523 Genomic DNA. Translation: CAM23957.1.
BC092543 mRNA. Translation: AAH92543.1.
CCDSiCCDS18148.1. [Q562D6-2]
RefSeqiNP_083362.1. NM_029086.2. [Q562D6-2]
XP_006538388.1. XM_006538325.2. [Q562D6-1]
UniGeneiMm.45973.

3D structure databases

ProteinModelPortaliQ562D6.
SMRiQ562D6. Positions 27-221.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi216996. 1 interaction.
IntActiQ562D6. 1 interaction.
MINTiMINT-8414996.

PTM databases

PhosphoSiteiQ562D6.

Proteomic databases

PRIDEiQ562D6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030015; ENSMUSP00000030015; ENSMUSG00000028331. [Q562D6-1]
ENSMUST00000086563; ENSMUSP00000083752; ENSMUSG00000028331. [Q562D6-2]
GeneIDi74753.
KEGGimmu:74753.
UCSCiuc008stq.1. mouse. [Q562D6-1]

Organism-specific databases

MGIiMGI:1922003. 5830415F09Rik.

Phylogenomic databases

eggNOGiCOG1720.
GeneTreeiENSGT00390000004643.
HOGENOMiHOG000231645.
HOVERGENiHBG054288.
InParanoidiQ562D6.
OMAiYEIACAP.
TreeFamiTF331670.

Miscellaneous databases

NextBioi341560.
PROiQ562D6.
SOURCEiSearch...

Gene expression databases

BgeeiQ562D6.
CleanExiMM_5830415F09RIK.
ExpressionAtlasiQ562D6. baseline and differential.
GenevisibleiQ562D6. MM.

Family and domain databases

Gene3Di2.40.30.70. 1 hit.
InterProiIPR023370. TsaA-like.
IPR001378. UPF0066.
IPR023368. UPF0066_cons_site.
[Graphical view]
PfamiPF01980. UPF0066. 1 hit.
[Graphical view]
SUPFAMiSSF118196. SSF118196. 2 hits.
TIGRFAMsiTIGR00104. tRNA_TsaA. 1 hit.
PROSITEiPS01318. TSAA_1. 1 hit.
PS51668. TSAA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
    Strain: C57BL/6J.
    Tissue: Brain cortex and Thymus.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Kidney.

Entry informationi

Entry nameiNAP1_MOUSE
AccessioniPrimary (citable) accession number: Q562D6
Secondary accession number(s): Q3U5H3, Q8BRJ8, Q9D3F5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: June 24, 2015
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.