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Protein

tRNA (adenine(37)-N6)-methyltransferase

Gene

Trmo

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase responsible for the addition of the methyl group in the formation of N6-methyl-N6-threonylcarbamoyladenosine at position 37 (m6t6A37) of the tRNA anticodon loop of tRNA(Ser)(GCU). The methyl group of m6t6A37 may improve the efficiency of the tRNA decoding ability. May bind to tRNA.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (adenine(37)-N6)-methyltransferase (EC:2.1.1.-By similarity)
Alternative name(s):
tRNA methyltransferase OImported
Gene namesi
Name:TrmoImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1922003 Trmo

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002888871 – 431tRNA (adenine(37)-N6)-methyltransferaseAdd BLAST431

Proteomic databases

PaxDbiQ562D6
PRIDEiQ562D6

PTM databases

iPTMnetiQ562D6
PhosphoSitePlusiQ562D6

Expressioni

Gene expression databases

BgeeiENSMUSG00000028331
CleanExiMM_5830415F09RIK
ExpressionAtlasiQ562D6 baseline and differential
GenevisibleiQ562D6 MM

Interactioni

Protein-protein interaction databases

BioGridi216996, 1 interactor
IntActiQ562D6, 1 interactor
MINTiQ562D6

Structurei

3D structure databases

ProteinModelPortaliQ562D6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 168TsaA-likePROSITE-ProRule annotationAdd BLAST139

Phylogenomic databases

eggNOGiKOG2942 Eukaryota
COG1720 LUCA
GeneTreeiENSGT00390000004643
HOGENOMiHOG000231645
HOVERGENiHBG054288
InParanoidiQ562D6
OMAiFKYFQSA
OrthoDBiEOG091G0DXG
TreeFamiTF331670

Family and domain databases

CDDicd09281 UPF0066, 1 hit
Gene3Di2.40.30.70, 1 hit
InterProiView protein in InterPro
IPR023368 UPF0066_cons_site
IPR023370 YaeB-like_N
IPR036413 YaeB-like_sf
IPR036414 YaeB_N_sf
PfamiView protein in Pfam
PF01980 UPF0066, 1 hit
SUPFAMiSSF118196 SSF118196, 2 hits
TIGRFAMsiTIGR00104 tRNA_TsaA, 1 hit
PROSITEiView protein in PROSITE
PS01318 TSAA_1, 1 hit
PS51668 TSAA_2, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q562D6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRGLEKQGSC ATAAPCGCVQ PALEAGNLLT EPIGYLESCF PAKIGTPRQP
60 70 80 90 100
SICSHSRACL KIRKNIFNNP EHSLMGLEEF SHVWILFVFH KNGHLNYKAK
110 120 130 140 150
VQPPRLNGAK TGVFSTRSPH RPNAIGLTLA KLEKVEGGAV YLSGVDMIDG
160 170 180 190 200
TPVLDIKPYI ADYDSPQNLS VHNDHHKLRA EAQVDGTANS CDQLLLSGRG
210 220 230 240 250
KVQPRQSTKE RPKCLEDRTS GENSQKSRDM SEIQHTLPED RERALDLALE
260 270 280 290 300
PSRGESMDMP ENQLGPPELK SFLEEGTDRP RKVEGALVLP GSSAETQWDA
310 320 330 340 350
SYRARTADRV PYSVVPSWVT EAPVAPLQVR FTPHAEMDLR KLNSGDASQP
360 370 380 390 400
SFKYFHSAEE AKRAIEAVLS ADPRSVYRRK LCEDRLFFFT VDTAHVTCWF
410 420 430
GDGFAEVVRI KLASESVQVA DPEESLAALG S
Length:431
Mass (Da):47,559
Last modified:May 29, 2007 - v2
Checksum:i5C98C96BE6A6692B
GO
Isoform 2 (identifier: Q562D6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     419-431: VADPEESLAALGS → TVLPPSPSPN...PPLPQGLVFS

Note: No experimental confirmation available.
Show »
Length:488
Mass (Da):53,677
Checksum:iA20D1CD4D29888AE
GO
Isoform 3 (identifier: Q562D6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-25: MRGLEKQGSCATAAPCGCVQPALEA → MWVRSAGPG

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.
Show »
Length:415
Mass (Da):46,027
Checksum:i893560AF2A75659B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22A → V in AAH92543 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0258151 – 25MRGLE…PALEA → MWVRSAGPG in isoform 3. 1 PublicationAdd BLAST25
Alternative sequenceiVSP_025816419 – 431VADPE…AALGS → TVLPPSPSPNGREEQSSVEE EVQQRAGPSRRAAPRAASML LTPQSSSLATSALTHWAISL PPLPQGLVFS in isoform 2. 1 PublicationAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017931 mRNA Translation: BAB31009.1
AK044073 mRNA Translation: BAC31763.1
AK153576 mRNA Translation: BAE32105.1
AL806523 Genomic DNA No translation available.
BC092543 mRNA Translation: AAH92543.1
CCDSiCCDS18148.1 [Q562D6-2]
CCDS84727.1 [Q562D6-1]
RefSeqiNP_001334024.1, NM_001347095.1 [Q562D6-1]
NP_083362.1, NM_029086.2 [Q562D6-2]
UniGeneiMm.45973

Genome annotation databases

EnsembliENSMUST00000030015; ENSMUSP00000030015; ENSMUSG00000028331 [Q562D6-1]
ENSMUST00000086563; ENSMUSP00000083752; ENSMUSG00000028331 [Q562D6-2]
GeneIDi74753
KEGGimmu:74753
UCSCiuc008stp.1 mouse [Q562D6-2]
uc008stq.1 mouse [Q562D6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTRMO_MOUSE
AccessioniPrimary (citable) accession number: Q562D6
Secondary accession number(s): Q3U5H3, Q8BRJ8, Q9D3F5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: May 23, 2018
This is version 83 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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