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Q562D6 (NAP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nef-associated protein 1

EC=3.1.2.-
Alternative name(s):
Thioesterase NAP1
Gene names
Name:Nap1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length431 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolyzes acyl-CoA thioesters (in vitro). Has a preference for substrates with medium chain length (C10-C14). Inactive towards substrates with C18 or C20 aliphatic chains. Its physiological substrate is not known By similarity.

Subunit structure

Interacts with lentivirus protein Nef By similarity.

Sequence similarities

Contains 1 TsaA-like domain.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functionhydrolase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q562D6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q562D6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     419-431: VADPEESLAALGS → TVLPPSPSPN...PPLPQGLVFS
Note: No experimental confirmation available.
Isoform 3 (identifier: Q562D6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-25: MRGLEKQGSCATAAPCGCVQPALEA → MWVRSAGPG
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 431431Nef-associated protein 1
PRO_0000288887

Regions

Domain30 – 168139TsaA-like

Natural variations

Alternative sequence1 – 2525MRGLE…PALEA → MWVRSAGPG in isoform 3.
VSP_025815
Alternative sequence419 – 43113VADPE…AALGS → TVLPPSPSPNGREEQSSVEE EVQQRAGPSRRAAPRAASML LTPQSSSLATSALTHWAISL PPLPQGLVFS in isoform 2.
VSP_025816

Experimental info

Sequence conflict221A → V in AAH92543. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 29, 2007. Version 2.
Checksum: 5C98C96BE6A6692B

FASTA43147,559
        10         20         30         40         50         60 
MRGLEKQGSC ATAAPCGCVQ PALEAGNLLT EPIGYLESCF PAKIGTPRQP SICSHSRACL 

        70         80         90        100        110        120 
KIRKNIFNNP EHSLMGLEEF SHVWILFVFH KNGHLNYKAK VQPPRLNGAK TGVFSTRSPH 

       130        140        150        160        170        180 
RPNAIGLTLA KLEKVEGGAV YLSGVDMIDG TPVLDIKPYI ADYDSPQNLS VHNDHHKLRA 

       190        200        210        220        230        240 
EAQVDGTANS CDQLLLSGRG KVQPRQSTKE RPKCLEDRTS GENSQKSRDM SEIQHTLPED 

       250        260        270        280        290        300 
RERALDLALE PSRGESMDMP ENQLGPPELK SFLEEGTDRP RKVEGALVLP GSSAETQWDA 

       310        320        330        340        350        360 
SYRARTADRV PYSVVPSWVT EAPVAPLQVR FTPHAEMDLR KLNSGDASQP SFKYFHSAEE 

       370        380        390        400        410        420 
AKRAIEAVLS ADPRSVYRRK LCEDRLFFFT VDTAHVTCWF GDGFAEVVRI KLASESVQVA 

       430 
DPEESLAALG S 

« Hide

Isoform 2 [UniParc].

Checksum: A20D1CD4D29888AE
Show »

FASTA48853,677
Isoform 3 [UniParc].

Checksum: 893560AF2A75659B
Show »

FASTA41546,027

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
Strain: C57BL/6J.
Tissue: Brain cortex and Thymus.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK017931 mRNA. Translation: BAB31009.1.
AK044073 mRNA. Translation: BAC31763.1.
AK153576 mRNA. Translation: BAE32105.1.
AL806523 Genomic DNA. Translation: CAM23957.1.
BC092543 mRNA. Translation: AAH92543.1.
RefSeqNP_083362.1. NM_029086.2.
XP_006538388.1. XM_006538325.1.
UniGeneMm.45973.

3D structure databases

ProteinModelPortalQ562D6.
SMRQ562D6. Positions 27-221.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ562D6. 1 interaction.
MINTMINT-8414996.

PTM databases

PhosphoSiteQ562D6.

Proteomic databases

PRIDEQ562D6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000030015; ENSMUSP00000030015; ENSMUSG00000028331. [Q562D6-1]
ENSMUST00000086563; ENSMUSP00000083752; ENSMUSG00000028331. [Q562D6-2]
GeneID74753.
KEGGmmu:74753.
UCSCuc008stq.1. mouse. [Q562D6-1]

Organism-specific databases

MGIMGI:1922003. 5830415F09Rik.

Phylogenomic databases

eggNOGCOG1720.
GeneTreeENSGT00390000004643.
HOGENOMHOG000231645.
HOVERGENHBG054288.
OMAYEIACAP.
TreeFamTF331670.

Gene expression databases

BgeeQ562D6.
CleanExMM_5830415F09RIK.
GenevestigatorQ562D6.

Family and domain databases

Gene3D2.40.30.70. 1 hit.
InterProIPR023370. TsaA-like.
IPR001378. UPF0066.
IPR023368. UPF0066_cons_site.
[Graphical view]
PfamPF01980. UPF0066. 1 hit.
[Graphical view]
SUPFAMSSF118196. SSF118196. 2 hits.
TIGRFAMsTIGR00104. YaeB_AF0241. 1 hit.
PROSITEPS01318. TSAA_1. 1 hit.
PS51668. TSAA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio341560.
PROQ562D6.
SOURCESearch...

Entry information

Entry nameNAP1_MOUSE
AccessionPrimary (citable) accession number: Q562D6
Secondary accession number(s): Q3U5H3, Q8BRJ8, Q9D3F5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: April 16, 2014
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot