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Protein

tRNA (adenine(37)-N6)-methyltransferase

Gene

Trmo

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase responsible for the addition of the methyl group in the formation of N6-methyl-N6-threonylcarbamoyladenosine at position 37 (m6t6A37) of the tRNA anticodon loop of tRNA(Ser)(GCU). The methyl group of m6t6A37 may improve the efficiency of the tRNA decoding ability. May bind to tRNA.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (adenine(37)-N6)-methyltransferase (EC:2.1.1.-By similarity)
Alternative name(s):
tRNA methyltransferase OImported
Gene namesi
Name:TrmoImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1922003. Trmo.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002888871 – 431tRNA (adenine(37)-N6)-methyltransferaseAdd BLAST431

Proteomic databases

PaxDbiQ562D6.
PRIDEiQ562D6.

PTM databases

iPTMnetiQ562D6.
PhosphoSitePlusiQ562D6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028331.
CleanExiMM_5830415F09RIK.
ExpressionAtlasiQ562D6. baseline and differential.
GenevisibleiQ562D6. MM.

Interactioni

Protein-protein interaction databases

BioGridi216996. 1 interactor.
IntActiQ562D6. 1 interactor.
MINTiMINT-8414996.

Structurei

3D structure databases

ProteinModelPortaliQ562D6.
SMRiQ562D6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 168TsaA-likePROSITE-ProRule annotationAdd BLAST139

Sequence similaritiesi

Contains 1 TsaA-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2942. Eukaryota.
COG1720. LUCA.
GeneTreeiENSGT00390000004643.
HOGENOMiHOG000231645.
HOVERGENiHBG054288.
InParanoidiQ562D6.
OMAiFKYFQSA.
OrthoDBiEOG091G0DXG.
TreeFamiTF331670.

Family and domain databases

CDDicd09281. UPF0066. 1 hit.
Gene3Di2.40.30.70. 1 hit.
InterProiIPR023370. TsaA-like.
IPR023368. UPF0066_cons_site.
[Graphical view]
PfamiPF01980. UPF0066. 1 hit.
[Graphical view]
SUPFAMiSSF118196. SSF118196. 2 hits.
TIGRFAMsiTIGR00104. tRNA_TsaA. 1 hit.
PROSITEiPS01318. TSAA_1. 1 hit.
PS51668. TSAA_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q562D6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRGLEKQGSC ATAAPCGCVQ PALEAGNLLT EPIGYLESCF PAKIGTPRQP
60 70 80 90 100
SICSHSRACL KIRKNIFNNP EHSLMGLEEF SHVWILFVFH KNGHLNYKAK
110 120 130 140 150
VQPPRLNGAK TGVFSTRSPH RPNAIGLTLA KLEKVEGGAV YLSGVDMIDG
160 170 180 190 200
TPVLDIKPYI ADYDSPQNLS VHNDHHKLRA EAQVDGTANS CDQLLLSGRG
210 220 230 240 250
KVQPRQSTKE RPKCLEDRTS GENSQKSRDM SEIQHTLPED RERALDLALE
260 270 280 290 300
PSRGESMDMP ENQLGPPELK SFLEEGTDRP RKVEGALVLP GSSAETQWDA
310 320 330 340 350
SYRARTADRV PYSVVPSWVT EAPVAPLQVR FTPHAEMDLR KLNSGDASQP
360 370 380 390 400
SFKYFHSAEE AKRAIEAVLS ADPRSVYRRK LCEDRLFFFT VDTAHVTCWF
410 420 430
GDGFAEVVRI KLASESVQVA DPEESLAALG S
Length:431
Mass (Da):47,559
Last modified:May 29, 2007 - v2
Checksum:i5C98C96BE6A6692B
GO
Isoform 2 (identifier: Q562D6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     419-431: VADPEESLAALGS → TVLPPSPSPN...PPLPQGLVFS

Note: No experimental confirmation available.
Show »
Length:488
Mass (Da):53,677
Checksum:iA20D1CD4D29888AE
GO
Isoform 3 (identifier: Q562D6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-25: MRGLEKQGSCATAAPCGCVQPALEA → MWVRSAGPG

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. No experimental confirmation available.
Show »
Length:415
Mass (Da):46,027
Checksum:i893560AF2A75659B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22A → V in AAH92543 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0258151 – 25MRGLE…PALEA → MWVRSAGPG in isoform 3. 1 PublicationAdd BLAST25
Alternative sequenceiVSP_025816419 – 431VADPE…AALGS → TVLPPSPSPNGREEQSSVEE EVQQRAGPSRRAAPRAASML LTPQSSSLATSALTHWAISL PPLPQGLVFS in isoform 2. 1 PublicationAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017931 mRNA. Translation: BAB31009.1.
AK044073 mRNA. Translation: BAC31763.1.
AK153576 mRNA. Translation: BAE32105.1.
AL806523 Genomic DNA. Translation: CAM23957.1.
BC092543 mRNA. Translation: AAH92543.1.
CCDSiCCDS18148.1. [Q562D6-2]
RefSeqiNP_083362.1. NM_029086.2. [Q562D6-2]
XP_006538388.1. XM_006538325.3. [Q562D6-1]
UniGeneiMm.45973.

Genome annotation databases

EnsembliENSMUST00000030015; ENSMUSP00000030015; ENSMUSG00000028331. [Q562D6-1]
ENSMUST00000086563; ENSMUSP00000083752; ENSMUSG00000028331. [Q562D6-2]
GeneIDi74753.
KEGGimmu:74753.
UCSCiuc008stp.1. mouse. [Q562D6-2]
uc008stq.1. mouse. [Q562D6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017931 mRNA. Translation: BAB31009.1.
AK044073 mRNA. Translation: BAC31763.1.
AK153576 mRNA. Translation: BAE32105.1.
AL806523 Genomic DNA. Translation: CAM23957.1.
BC092543 mRNA. Translation: AAH92543.1.
CCDSiCCDS18148.1. [Q562D6-2]
RefSeqiNP_083362.1. NM_029086.2. [Q562D6-2]
XP_006538388.1. XM_006538325.3. [Q562D6-1]
UniGeneiMm.45973.

3D structure databases

ProteinModelPortaliQ562D6.
SMRiQ562D6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi216996. 1 interactor.
IntActiQ562D6. 1 interactor.
MINTiMINT-8414996.

PTM databases

iPTMnetiQ562D6.
PhosphoSitePlusiQ562D6.

Proteomic databases

PaxDbiQ562D6.
PRIDEiQ562D6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030015; ENSMUSP00000030015; ENSMUSG00000028331. [Q562D6-1]
ENSMUST00000086563; ENSMUSP00000083752; ENSMUSG00000028331. [Q562D6-2]
GeneIDi74753.
KEGGimmu:74753.
UCSCiuc008stp.1. mouse. [Q562D6-2]
uc008stq.1. mouse. [Q562D6-1]

Organism-specific databases

CTDi51531.
MGIiMGI:1922003. Trmo.

Phylogenomic databases

eggNOGiKOG2942. Eukaryota.
COG1720. LUCA.
GeneTreeiENSGT00390000004643.
HOGENOMiHOG000231645.
HOVERGENiHBG054288.
InParanoidiQ562D6.
OMAiFKYFQSA.
OrthoDBiEOG091G0DXG.
TreeFamiTF331670.

Miscellaneous databases

PROiQ562D6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028331.
CleanExiMM_5830415F09RIK.
ExpressionAtlasiQ562D6. baseline and differential.
GenevisibleiQ562D6. MM.

Family and domain databases

CDDicd09281. UPF0066. 1 hit.
Gene3Di2.40.30.70. 1 hit.
InterProiIPR023370. TsaA-like.
IPR023368. UPF0066_cons_site.
[Graphical view]
PfamiPF01980. UPF0066. 1 hit.
[Graphical view]
SUPFAMiSSF118196. SSF118196. 2 hits.
TIGRFAMsiTIGR00104. tRNA_TsaA. 1 hit.
PROSITEiPS01318. TSAA_1. 1 hit.
PS51668. TSAA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMO_MOUSE
AccessioniPrimary (citable) accession number: Q562D6
Secondary accession number(s): Q3U5H3, Q8BRJ8, Q9D3F5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: November 2, 2016
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.