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Protein

1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase

Gene

Adi1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of formate and 2-keto-4-methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene). Also down-regulates cell migration mediated by MMP14 (By similarity). Induces apoptosis when overexpressed.UniRule annotation

Catalytic activityi

1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 = 4-(methylthio)-2-oxobutanoate + formate.UniRule annotation

Cofactori

Fe cationUniRule annotationNote: Binds 1 Fe cation per monomer. Can also use other divalent metal cations.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 5 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Methylthioribose-1-phosphate isomerase (Mri1)
  2. Methylthioribulose-1-phosphate dehydratase (Apip)
  3. Enolase-phosphatase E1 (Enoph1)
  4. Enolase-phosphatase E1 (Enoph1)
  5. 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (Adi1)
  6. no protein annotated in this organism
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi88 – 881Iron or nickelUniRule annotation
Metal bindingi90 – 901Iron or nickelUniRule annotation
Metal bindingi94 – 941Iron or nickelUniRule annotation
Metal bindingi133 – 1331Iron or nickelUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-1237112. Methionine salvage pathway.
UniPathwayiUPA00904; UER00878.

Names & Taxonomyi

Protein namesi
Recommended name:
1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenaseUniRule annotation (EC:1.13.11.54UniRule annotation)
Alternative name(s):
Acireductone dioxygenase (Fe(2+)-requiring)UniRule annotation
Short name:
ARDUniRule annotation
Short name:
Fe-ARDUniRule annotation
Androgen-responsive ARD-like protein 1
Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1UniRule annotation
Short name:
MTCBP-1UniRule annotation
Gene namesi
Name:Adi1
Synonyms:Alp1, Mtcbp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi727828. Adi1.

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Nucleus UniRule annotation1 Publication
  • Cell membrane UniRule annotation1 Publication; Peripheral membrane protein UniRule annotation1 Publication; Cytoplasmic side UniRule annotation1 Publication

  • Note: Localizes to the plasma membrane when complexed to MMP14.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1791791,2-dihydroxy-3-keto-5-methylthiopentene dioxygenasePRO_0000162944Add
BLAST

Proteomic databases

PaxDbiQ562C9.
PRIDEiQ562C9.

Expressioni

Tissue specificityi

Detected in prostate, liver, heart, brain, muscle, kidney and seminal vesicles.1 Publication

Inductioni

Up-regulated by androgens in the prostate, but not in the other tissues tested.1 Publication

Gene expression databases

GenevisibleiQ562C9. RN.

Interactioni

Subunit structurei

Monomer. Interacts with MMP14.UniRule annotation

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011952.

Structurei

3D structure databases

ProteinModelPortaliQ562C9.
SMRiQ562C9. Positions 1-179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acireductone dioxygenase (ARD) family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2107. Eukaryota.
COG1791. LUCA.
GeneTreeiENSGT00390000008195.
HOGENOMiHOG000201071.
HOVERGENiHBG081992.
InParanoidiQ562C9.
KOiK08967.
OMAiMVRAWYM.
OrthoDBiEOG73FQP0.
PhylomeDBiQ562C9.
TreeFamiTF300231.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_03154. Salvage_MtnD_euk.
InterProiIPR004313. ARD.
IPR027496. ARD_euk.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR23418. PTHR23418. 1 hit.
PfamiPF03079. ARD. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

Q562C9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVQAWYMDES TADPRMPHRA QPDRPVGLEQ LRTLGVLYWK LDADKYENDP
60 70 80 90 100
ELEQIRKTRN YSWMDIITIC KDSLPNYEEK IKMFFEEHLH LDEEIRYILE
110 120 130 140 150
GSGYFDVRDK EDKWIRISME KGDMITLPAG IYHRFTLDEK NYVKAMRLFV
160 170
GEPVWTPYNR PADHFDARVQ YVKFLEGTA
Length:179
Mass (Da):21,461
Last modified:May 10, 2005 - v1
Checksum:i160E7A28A263A21A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti161 – 1611P → A in AAQ24524 (PubMed:14684610).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY346335 mRNA. Translation: AAQ24524.1.
BC092562 mRNA. Translation: AAH92562.1.
RefSeqiNP_954528.1. NM_199097.1.
UniGeneiRn.12694.

Genome annotation databases

EnsembliENSRNOT00000011951; ENSRNOP00000011952; ENSRNOG00000008950.
GeneIDi298934.
KEGGirno:298934.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY346335 mRNA. Translation: AAQ24524.1.
BC092562 mRNA. Translation: AAH92562.1.
RefSeqiNP_954528.1. NM_199097.1.
UniGeneiRn.12694.

3D structure databases

ProteinModelPortaliQ562C9.
SMRiQ562C9. Positions 1-179.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011952.

Proteomic databases

PaxDbiQ562C9.
PRIDEiQ562C9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000011951; ENSRNOP00000011952; ENSRNOG00000008950.
GeneIDi298934.
KEGGirno:298934.

Organism-specific databases

CTDi55256.
RGDi727828. Adi1.

Phylogenomic databases

eggNOGiKOG2107. Eukaryota.
COG1791. LUCA.
GeneTreeiENSGT00390000008195.
HOGENOMiHOG000201071.
HOVERGENiHBG081992.
InParanoidiQ562C9.
KOiK08967.
OMAiMVRAWYM.
OrthoDBiEOG73FQP0.
PhylomeDBiQ562C9.
TreeFamiTF300231.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00878.
ReactomeiR-RNO-1237112. Methionine salvage pathway.

Miscellaneous databases

NextBioi644526.
PROiQ562C9.

Gene expression databases

GenevisibleiQ562C9. RN.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_03154. Salvage_MtnD_euk.
InterProiIPR004313. ARD.
IPR027496. ARD_euk.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR23418. PTHR23418. 1 hit.
PfamiPF03079. ARD. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of an androgen-responsive gene encoding an aci-reductone dioxygenase-like protein in the rat prostate."
    Oram S., Jiang F., Cai X., Haleem R., Dincer Z., Wang Z.
    Endocrinology 145:1933-1942(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Prostate.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiMTND_RAT
AccessioniPrimary (citable) accession number: Q562C9
Secondary accession number(s): Q6V9S4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: May 10, 2005
Last modified: May 11, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.