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Protein

Pumilio homolog 3

Gene

Pum3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Inhibits the poly(ADP-ribosyl)ation activity of PARP1 and the degradation of PARP1 by CASP3 following genotoxic stress. Binds to double-stranded RNA or DNA without sequence specificity. Involved in development of the eye and of primordial germ cells.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

DNA-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pumilio homolog 3Curated
Gene namesi
Name:Pum3Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1564753. Pum3.

Subcellular locationi

  • Nucleusnucleolus By similarity
  • Nucleusnucleoplasm By similarity

  • Note: Localizes predominantly in the nucleolus with minor punctate signals in the nucleoplasm.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 647647Pumilio homolog 3PRO_0000075931Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei33 – 331N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ562C7.
PRIDEiQ562C7.

PTM databases

iPTMnetiQ562C7.
PhosphoSiteiQ562C7.

Expressioni

Gene expression databases

GenevisibleiQ562C7. RN.

Interactioni

Subunit structurei

Interacts with PARP1 (via catalytic domain).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000034834.

Structurei

3D structure databases

ProteinModelPortaliQ562C7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini142 – 509368PUM-HDPROSITE-ProRule annotationAdd
BLAST
Repeati176 – 21136Pumilio 1By similarityAdd
BLAST
Repeati212 – 24736Pumilio 2By similarityAdd
BLAST
Repeati248 – 27629Pumilio 3By similarityAdd
BLAST
Repeati288 – 32437Pumilio 4By similarityAdd
BLAST
Repeati325 – 36036Pumilio 5By similarityAdd
BLAST
Repeati361 – 39636Pumilio 6By similarityAdd
BLAST
Repeati397 – 43438Pumilio 7By similarityAdd
BLAST
Repeati435 – 50369Pumilio 8By similarityAdd
BLAST
Repeati504 – 55047Pumilio 9By similarityAdd
BLAST
Repeati551 – 59545Pumilio 10By similarityAdd
BLAST
Repeati596 – 63540Pumilio 11By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi105 – 11713Nuclear localization signalBy similarityAdd
BLAST

Domaini

A 90 degree bend between Pumilio repeats 3 and 4 gives rise to a L-shaped protein.By similarity

Sequence similaritiesi

Contains 1 PUM-HD domain.PROSITE-ProRule annotation
Contains 11 pumilio repeats.By similarity

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2050. Eukaryota.
ENOG410XSHY. LUCA.
GeneTreeiENSGT00390000015757.
HOGENOMiHOG000237322.
HOVERGENiHBG052166.
InParanoidiQ562C7.
KOiK14844.
OMAiYLQEHAQ.
OrthoDBiEOG783MV2.
PhylomeDBiQ562C7.
TreeFamiTF312954.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR012959. CPL_dom.
IPR033133. PUM-HD.
IPR001313. Pumilio_RNA-bd_rpt.
[Graphical view]
PfamiPF08144. CPL. 1 hit.
[Graphical view]
SMARTiSM00025. Pumilio. 6 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS50302. PUM. 5 hits.
PS50303. PUM_HD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q562C7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVKGKKKIT GKSPQTSQGK NKFHKNSESS SAKAFPRKAA KEGGPKVTSK
60 70 80 90 100
NFEKGATKPG KKRVKQFKNK PQGGKGPKDK FQKANKFNKK RKFQPDGKSD
110 120 130 140 150
ESAAKKPKWD DFKKKKKELK QNRQLSDKTN YDVVVRAKHI WESLRRKDCD
160 170 180 190 200
KEKRVKLMSD LQKLIQGKIK TIAFAHDSTR VIQCLIQYGS EEQRKWAFEE
210 220 230 240 250
LQGDLVELSK AKYSRNIVKK FLMYGSKPQI AEIIRSFKGH VRKMLRHSEA
260 270 280 290 300
SAIVEYAYND KAILEQRNML TEELYGNTFQ LYKSADHPTL EKVLEVQPGK
310 320 330 340 350
LELILDEMKQ ILTPMAQKEA VIKHSLVHKV FLDFFTYAPP KLRSELIEAI
360 370 380 390 400
REAVVYLAHT HDGARVAMHC LWHGTPKDRK VIVKTMKTYV EKIANGQYSH
410 420 430 440 450
LVLLAAFDCI DDTKLVKQII ISEVISSLPS IVNDKYGRKV LLYLLSPRAP
460 470 480 490 500
AHLVPEIIQL LQKGDGNAHS KKDTAIRRRE LLESISPALL SYLQGHTREV
510 520 530 540 550
VLDKSVCVLV SDILGSATGD AQPAMDAIAG LAAEELYPGG KDGELHIAEH
560 570 580 590 600
PAGHLVLKWL IEQDKKIKEN GKEGCFAKTL VECVGMKNLK SWASINRGAI
610 620 630 640
VLSSLLQSCD QDVVNKVKAG LKTLIPTLEK TKSTSKGIQT LLEKLTA
Length:647
Mass (Da):72,786
Last modified:May 10, 2005 - v1
Checksum:i59AFDE6F6D502AC6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC092579 mRNA. Translation: AAH92579.1.
BC098744 mRNA. Translation: AAH98744.2.
RefSeqiNP_001020207.1. NM_001025036.1.
XP_006231308.1. XM_006231246.2.
UniGeneiRn.127943.

Genome annotation databases

EnsembliENSRNOT00000033724; ENSRNOP00000034834; ENSRNOG00000012574.
GeneIDi499339.
KEGGirno:499339.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC092579 mRNA. Translation: AAH92579.1.
BC098744 mRNA. Translation: AAH98744.2.
RefSeqiNP_001020207.1. NM_001025036.1.
XP_006231308.1. XM_006231246.2.
UniGeneiRn.127943.

3D structure databases

ProteinModelPortaliQ562C7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000034834.

PTM databases

iPTMnetiQ562C7.
PhosphoSiteiQ562C7.

Proteomic databases

PaxDbiQ562C7.
PRIDEiQ562C7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000033724; ENSRNOP00000034834; ENSRNOG00000012574.
GeneIDi499339.
KEGGirno:499339.

Organism-specific databases

CTDi9933.
RGDi1564753. Pum3.

Phylogenomic databases

eggNOGiKOG2050. Eukaryota.
ENOG410XSHY. LUCA.
GeneTreeiENSGT00390000015757.
HOGENOMiHOG000237322.
HOVERGENiHBG052166.
InParanoidiQ562C7.
KOiK14844.
OMAiYLQEHAQ.
OrthoDBiEOG783MV2.
PhylomeDBiQ562C7.
TreeFamiTF312954.

Miscellaneous databases

PROiQ562C7.

Gene expression databases

GenevisibleiQ562C7. RN.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR012959. CPL_dom.
IPR033133. PUM-HD.
IPR001313. Pumilio_RNA-bd_rpt.
[Graphical view]
PfamiPF08144. CPL. 1 hit.
[Graphical view]
SMARTiSM00025. Pumilio. 6 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS50302. PUM. 5 hits.
PS50303. PUM_HD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver and Thymus.

Entry informationi

Entry nameiPUM3_RAT
AccessioniPrimary (citable) accession number: Q562C7
Secondary accession number(s): Q4G054
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: May 10, 2005
Last modified: June 8, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.