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Q562B5

- PGAM5_RAT

UniProt

Q562B5 - PGAM5_RAT

Protein

Serine/threonine-protein phosphatase PGAM5, mitochondrial

Gene

Pgam5

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (10 May 2005)
      Previous versions | rss
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    Functioni

    Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression By similarity. Acts as a central mediator for programmed necrosis induced by TNF, by reactive oxygen species and by calcium ionophore.By similarity1 Publication

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    GO - Molecular functioni

    1. GTPase activator activity Source: UniProtKB
    2. phosphatase activity Source: UniProtKB
    3. phosphoprotein phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. dephosphorylation Source: UniProtKB
    2. necroptotic process Source: UniProtKB
    3. positive regulation of GTPase activity Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Necrosis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase PGAM5, mitochondrial (EC:3.1.3.16)
    Alternative name(s):
    Phosphoglycerate mutase family member 5
    Gene namesi
    Name:Pgam5
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi1312028. Pgam5.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial outer membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion outer membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 288288Serine/threonine-protein phosphatase PGAM5, mitochondrialPRO_0000288784Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei115 – 1151N6-acetyllysineBy similarity
    Modified residuei143 – 1431N6-acetyllysineBy similarity
    Modified residuei190 – 1901N6-acetyllysineBy similarity

    Post-translational modificationi

    Phosphorylated by the RIPK1/RIPK3 complex under necrotic conditions. This phosphorylation increases PGAM5 phosphatase activity By similarity.By similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ562B5.
    PRIDEiQ562B5.

    Expressioni

    Gene expression databases

    GenevestigatoriQ562B5.

    Interactioni

    Subunit structurei

    Dimer. Forms a ternary complex with NFE2L2 and KEAP1. Interacts with BCL2L1 and MAP3K5 By similarity. Upon TNF-induced necrosis, forms in complex with RIPK1, RIPK3 and MLKL; the formation of this complex leads to PGAM5 phosphorylation By similarity. Interacts with DNM1L; this interaction leads to DNM1L dephosphorylation and activation and eventually to mitochondria fragmentation By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000053513.

    Structurei

    3D structure databases

    ProteinModelPortaliQ562B5.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2923HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni76 – 816Interaction with KEAP1By similarity

    Domaini

    The N-terminal 35 amino acids, including the potential transmembrane alpha-helix, function as a non-cleaved mitochondrial targeting sequence that targets the protein to the cytosolic side of the outer mitochondrial membrane.By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG71348.
    HOGENOMiHOG000261217.
    HOVERGENiHBG105576.
    InParanoidiQ562B5.
    KOiK15637.
    PhylomeDBiQ562B5.

    Family and domain databases

    Gene3Di3.40.50.1240. 1 hit.
    InterProiIPR013078. His_Pase_superF_clade-1.
    IPR029033. His_PPase_superfam.
    [Graphical view]
    PfamiPF00300. His_Phos_1. 1 hit.
    [Graphical view]
    SMARTiSM00855. PGAM. 1 hit.
    [Graphical view]
    SUPFAMiSSF53254. SSF53254. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q562B5-1 [UniParc]FASTAAdd to Basket

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    MAFRQALQLA ACGLAGGSAA VLFSAVAVGK PRAGGDADTR TTEPLAWTGA    50
    RPGHGVWDSN WDRREPLSLI NLKKRNVEFG EDELASRLDH YKAKATRHIF 100
    LIRHSQYNVD GSMEKDRTLT PLGREQAELT GIRLASLGLK FNKIVHSSMT 150
    RAVETTDIIS KHLPGVCRVS TDLLREGAPI EPDPPVSHWK PEAVQYYEDG 200
    ARIEAAFRNY IHRADAKQEE DSYEIFICHA NVIRYIVCRA LQFPPEGWLR 250
    LSLNNGSITH LVIRPNGRVA LRTLGDTGFM PPDKITRS 288
    Length:288
    Mass (Da):32,060
    Last modified:May 10, 2005 - v1
    Checksum:iB4DD3F3D276A61C5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC092607 mRNA. Translation: AAH92607.1.
    RefSeqiNP_001020443.1. NM_001025272.1.
    UniGeneiRn.23489.

    Genome annotation databases

    GeneIDi288731.
    KEGGirno:288731.
    UCSCiRGD:1312028. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC092607 mRNA. Translation: AAH92607.1 .
    RefSeqi NP_001020443.1. NM_001025272.1.
    UniGenei Rn.23489.

    3D structure databases

    ProteinModelPortali Q562B5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000053513.

    Proteomic databases

    PaxDbi Q562B5.
    PRIDEi Q562B5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 288731.
    KEGGi rno:288731.
    UCSCi RGD:1312028. rat.

    Organism-specific databases

    CTDi 192111.
    RGDi 1312028. Pgam5.

    Phylogenomic databases

    eggNOGi NOG71348.
    HOGENOMi HOG000261217.
    HOVERGENi HBG105576.
    InParanoidi Q562B5.
    KOi K15637.
    PhylomeDBi Q562B5.

    Miscellaneous databases

    NextBioi 628617.

    Gene expression databases

    Genevestigatori Q562B5.

    Family and domain databases

    Gene3Di 3.40.50.1240. 1 hit.
    InterProi IPR013078. His_Pase_superF_clade-1.
    IPR029033. His_PPase_superfam.
    [Graphical view ]
    Pfami PF00300. His_Phos_1. 1 hit.
    [Graphical view ]
    SMARTi SM00855. PGAM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53254. SSF53254. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Ovary.
    2. "The mitochondrial phosphatase PGAM5 functions at the convergence point of multiple necrotic death pathways."
      Wang Z., Jiang H., Chen S., Du F., Wang X.
      Cell 148:228-243(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiPGAM5_RAT
    AccessioniPrimary (citable) accession number: Q562B5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: May 10, 2005
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3