Q562B5 (PGAM5_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase PGAM5, mitochondrial
EC=3.1.3.16

Alternative name(s):
Phosphoglycerate mutase family member 5

Gene names

Name:

Pgam5

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression By similarity. Acts as a central mediator for programmed necrosis induced by TNF, by reactive oxygen species and by calcium ionophore. Ref.2
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Subunit structure	Dimer. Forms a ternary complex with NFE2L2 and KEAP1. Interacts with BCL2L1 and MAP3K5 By similarity. Upon TNF-induced necrosis, forms in complex with RIPK1, RIPK3 and MLKL; the formation of this complex leads to PGAM5 phosphorylation By similarity. Interacts with DNM1L; this interaction leads to DNM1L dephosphorylation and activation and eventually to mitochondria fragmentation By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein By similarity.
Domain	The N-terminal 35 amino acids, including the potential transmembrane alpha-helix, function as a non-cleaved mitochondrial targeting sequence that targets the protein to the cytosolic side of the outer mitochondrial membrane By similarity.
Post-translational modification	Phosphorylated by the RIPK1/RIPK3 complex under necrotic conditions. This phosphorylation increases PGAM5 phosphatase activity By similarity.
Sequence similarities	Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.

Ontologies

Keywords
Biological process	Necrosis
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	phosphoprotein phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	Serine/threonine-protein phosphatase PGAM5, mitochondrial		PRO_0000288784
Regions
	Transmembrane	7 – 29	23	Helical; Potential
	Region	76 – 81	6	Interaction with KEAP1 By similarity
Amino acid modifications
	Modified residue	115	1	N6-acetyllysine By similarity
	Modified residue	143	1	N6-acetyllysine By similarity
	Modified residue	190	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q562B5 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: B4DD3F3D276A61C5	FASTA	288	32,060
	10 20 30 40 50 60 MAFRQALQLA ACGLAGGSAA VLFSAVAVGK PRAGGDADTR TTEPLAWTGA RPGHGVWDSN 70 80 90 100 110 120 WDRREPLSLI NLKKRNVEFG EDELASRLDH YKAKATRHIF LIRHSQYNVD GSMEKDRTLT 130 140 150 160 170 180 PLGREQAELT GIRLASLGLK FNKIVHSSMT RAVETTDIIS KHLPGVCRVS TDLLREGAPI 190 200 210 220 230 240 EPDPPVSHWK PEAVQYYEDG ARIEAAFRNY IHRADAKQEE DSYEIFICHA NVIRYIVCRA 250 260 270 280 LQFPPEGWLR LSLNNGSITH LVIRPNGRVA LRTLGDTGFM PPDKITRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
[2]	"The mitochondrial phosphatase PGAM5 functions at the convergence point of multiple necrotic death pathways." Wang Z., Jiang H., Chen S., Du F., Wang X. Cell 148:228-243(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC092607 mRNA. Translation: AAH92607.1.
IPI	IPI00392594.
RefSeq	NP_001020443.1. NM_001025272.1.
UniGene	Rn.23489.
3D structure databases
ProteinModelPortal	Q562B5.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000053513.
Proteomic databases
PaxDb	Q562B5.
PRIDE	Q562B5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	288731.
KEGG	rno:288731.
UCSC	RGD:1312028. rat.
Organism-specific databases
CTD	192111.
RGD	1312028. Pgam5.
Phylogenomic databases
eggNOG	NOG71348.
HOGENOM	HOG000261217.
HOVERGEN	HBG105576.
InParanoid	Q562B5.
KO	K15637.
OrthoDB	EOG4T4CW2.
Gene expression databases
Genevestigator	Q562B5.
Family and domain databases
InterPro	IPR013078. His_Pase_superF_clade-1. [Graphical view]
Pfam	PF00300. His_Phos_1. 1 hit. [Graphical view]
SMART	SM00855. PGAM. 1 hit. [Graphical view]
PROSITE	PS00175. PG_MUTASE. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	628617.

Entry information

Entry name

PGAM5_RAT

Accession

Primary (citable) accession number: Q562B5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	May 10, 2005
Last modified:	April 3, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program