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Protein

Serine/threonine-protein phosphatase PGAM5, mitochondrial

Gene

Pgam5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression (By similarity). Acts as a central mediator for programmed necrosis induced by TNF, by reactive oxygen species and by calcium ionophore.By similarity1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

GO - Molecular functioni

  1. GTPase activator activity Source: UniProtKB
  2. phosphatase activity Source: UniProtKB
  3. phosphoprotein phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. dephosphorylation Source: UniProtKB
  2. necroptotic process Source: UniProtKB
  3. positive regulation of GTPase activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Necrosis

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PGAM5, mitochondrial (EC:3.1.3.16)
Alternative name(s):
Phosphoglycerate mutase family member 5
Gene namesi
Name:Pgam5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1312028. Pgam5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei7 – 2923HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial outer membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 288288Serine/threonine-protein phosphatase PGAM5, mitochondrialPRO_0000288784Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei115 – 1151N6-acetyllysineBy similarity
Modified residuei143 – 1431N6-acetyllysineBy similarity
Modified residuei190 – 1901N6-acetyllysineBy similarity

Post-translational modificationi

Phosphorylated by the RIPK1/RIPK3 complex under necrotic conditions. This phosphorylation increases PGAM5 phosphatase activity (By similarity).By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ562B5.
PRIDEiQ562B5.

Expressioni

Gene expression databases

GenevestigatoriQ562B5.

Interactioni

Subunit structurei

Dimer. Forms a ternary complex with NFE2L2 and KEAP1. Interacts with BCL2L1 and MAP3K5 (By similarity). Upon TNF-induced necrosis, forms in complex with RIPK1, RIPK3 and MLKL; the formation of this complex leads to PGAM5 phosphorylation (By similarity). Interacts with DNM1L; this interaction leads to DNM1L dephosphorylation and activation and eventually to mitochondria fragmentation (By similarity).By similarity

Protein-protein interaction databases

BioGridi252666. 1 interaction.
STRINGi10116.ENSRNOP00000053513.

Structurei

3D structure databases

ProteinModelPortaliQ562B5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni76 – 816Interaction with KEAP1By similarity

Domaini

The N-terminal 35 amino acids, including the potential transmembrane alpha-helix, function as a non-cleaved mitochondrial targeting sequence that targets the protein to the cytosolic side of the outer mitochondrial membrane.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG71348.
HOGENOMiHOG000261217.
HOVERGENiHBG105576.
InParanoidiQ562B5.
KOiK15637.
PhylomeDBiQ562B5.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00300. His_Phos_1. 1 hit.
[Graphical view]
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.

Sequencei

Sequence statusi: Complete.

Q562B5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFRQALQLA ACGLAGGSAA VLFSAVAVGK PRAGGDADTR TTEPLAWTGA
60 70 80 90 100
RPGHGVWDSN WDRREPLSLI NLKKRNVEFG EDELASRLDH YKAKATRHIF
110 120 130 140 150
LIRHSQYNVD GSMEKDRTLT PLGREQAELT GIRLASLGLK FNKIVHSSMT
160 170 180 190 200
RAVETTDIIS KHLPGVCRVS TDLLREGAPI EPDPPVSHWK PEAVQYYEDG
210 220 230 240 250
ARIEAAFRNY IHRADAKQEE DSYEIFICHA NVIRYIVCRA LQFPPEGWLR
260 270 280
LSLNNGSITH LVIRPNGRVA LRTLGDTGFM PPDKITRS
Length:288
Mass (Da):32,060
Last modified:May 9, 2005 - v1
Checksum:iB4DD3F3D276A61C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC092607 mRNA. Translation: AAH92607.1.
RefSeqiNP_001020443.1. NM_001025272.1.
UniGeneiRn.23489.

Genome annotation databases

GeneIDi288731.
KEGGirno:288731.
UCSCiRGD:1312028. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC092607 mRNA. Translation: AAH92607.1.
RefSeqiNP_001020443.1. NM_001025272.1.
UniGeneiRn.23489.

3D structure databases

ProteinModelPortaliQ562B5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi252666. 1 interaction.
STRINGi10116.ENSRNOP00000053513.

Proteomic databases

PaxDbiQ562B5.
PRIDEiQ562B5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi288731.
KEGGirno:288731.
UCSCiRGD:1312028. rat.

Organism-specific databases

CTDi192111.
RGDi1312028. Pgam5.

Phylogenomic databases

eggNOGiNOG71348.
HOGENOMiHOG000261217.
HOVERGENiHBG105576.
InParanoidiQ562B5.
KOiK15637.
PhylomeDBiQ562B5.

Miscellaneous databases

NextBioi628617.

Gene expression databases

GenevestigatoriQ562B5.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00300. His_Phos_1. 1 hit.
[Graphical view]
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.
  2. "The mitochondrial phosphatase PGAM5 functions at the convergence point of multiple necrotic death pathways."
    Wang Z., Jiang H., Chen S., Du F., Wang X.
    Cell 148:228-243(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPGAM5_RAT
AccessioniPrimary (citable) accession number: Q562B5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 28, 2007
Last sequence update: May 9, 2005
Last modified: March 3, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.