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Q562B5

- PGAM5_RAT

UniProt

Q562B5 - PGAM5_RAT

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Protein

Serine/threonine-protein phosphatase PGAM5, mitochondrial

Gene

Pgam5

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression (By similarity). Acts as a central mediator for programmed necrosis induced by TNF, by reactive oxygen species and by calcium ionophore.By similarity1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

GO - Molecular functioni

  1. GTPase activator activity Source: UniProtKB
  2. phosphatase activity Source: UniProtKB
  3. phosphoprotein phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. dephosphorylation Source: UniProtKB
  2. necroptotic process Source: UniProtKB
  3. positive regulation of GTPase activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Necrosis

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PGAM5, mitochondrial (EC:3.1.3.16)
Alternative name(s):
Phosphoglycerate mutase family member 5
Gene namesi
Name:Pgam5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi1312028. Pgam5.

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial outer membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 288288Serine/threonine-protein phosphatase PGAM5, mitochondrialPRO_0000288784Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei115 – 1151N6-acetyllysineBy similarity
Modified residuei143 – 1431N6-acetyllysineBy similarity
Modified residuei190 – 1901N6-acetyllysineBy similarity

Post-translational modificationi

Phosphorylated by the RIPK1/RIPK3 complex under necrotic conditions. This phosphorylation increases PGAM5 phosphatase activity (By similarity).By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ562B5.
PRIDEiQ562B5.

Expressioni

Gene expression databases

GenevestigatoriQ562B5.

Interactioni

Subunit structurei

Dimer. Forms a ternary complex with NFE2L2 and KEAP1. Interacts with BCL2L1 and MAP3K5 (By similarity). Upon TNF-induced necrosis, forms in complex with RIPK1, RIPK3 and MLKL; the formation of this complex leads to PGAM5 phosphorylation (By similarity). Interacts with DNM1L; this interaction leads to DNM1L dephosphorylation and activation and eventually to mitochondria fragmentation (By similarity).By similarity

Protein-protein interaction databases

BioGridi252666. 1 interaction.
STRINGi10116.ENSRNOP00000053513.

Structurei

3D structure databases

ProteinModelPortaliQ562B5.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei7 – 2923HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni76 – 816Interaction with KEAP1By similarity

Domaini

The N-terminal 35 amino acids, including the potential transmembrane alpha-helix, function as a non-cleaved mitochondrial targeting sequence that targets the protein to the cytosolic side of the outer mitochondrial membrane.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG71348.
HOGENOMiHOG000261217.
HOVERGENiHBG105576.
InParanoidiQ562B5.
KOiK15637.
PhylomeDBiQ562B5.

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
[Graphical view]
PfamiPF00300. His_Phos_1. 1 hit.
[Graphical view]
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF53254. SSF53254. 1 hit.

Sequencei

Sequence statusi: Complete.

Q562B5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAFRQALQLA ACGLAGGSAA VLFSAVAVGK PRAGGDADTR TTEPLAWTGA
60 70 80 90 100
RPGHGVWDSN WDRREPLSLI NLKKRNVEFG EDELASRLDH YKAKATRHIF
110 120 130 140 150
LIRHSQYNVD GSMEKDRTLT PLGREQAELT GIRLASLGLK FNKIVHSSMT
160 170 180 190 200
RAVETTDIIS KHLPGVCRVS TDLLREGAPI EPDPPVSHWK PEAVQYYEDG
210 220 230 240 250
ARIEAAFRNY IHRADAKQEE DSYEIFICHA NVIRYIVCRA LQFPPEGWLR
260 270 280
LSLNNGSITH LVIRPNGRVA LRTLGDTGFM PPDKITRS
Length:288
Mass (Da):32,060
Last modified:May 10, 2005 - v1
Checksum:iB4DD3F3D276A61C5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC092607 mRNA. Translation: AAH92607.1.
RefSeqiNP_001020443.1. NM_001025272.1.
UniGeneiRn.23489.

Genome annotation databases

GeneIDi288731.
KEGGirno:288731.
UCSCiRGD:1312028. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC092607 mRNA. Translation: AAH92607.1 .
RefSeqi NP_001020443.1. NM_001025272.1.
UniGenei Rn.23489.

3D structure databases

ProteinModelPortali Q562B5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 252666. 1 interaction.
STRINGi 10116.ENSRNOP00000053513.

Proteomic databases

PaxDbi Q562B5.
PRIDEi Q562B5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 288731.
KEGGi rno:288731.
UCSCi RGD:1312028. rat.

Organism-specific databases

CTDi 192111.
RGDi 1312028. Pgam5.

Phylogenomic databases

eggNOGi NOG71348.
HOGENOMi HOG000261217.
HOVERGENi HBG105576.
InParanoidi Q562B5.
KOi K15637.
PhylomeDBi Q562B5.

Miscellaneous databases

NextBioi 628617.

Gene expression databases

Genevestigatori Q562B5.

Family and domain databases

Gene3Di 3.40.50.1240. 1 hit.
InterProi IPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
[Graphical view ]
Pfami PF00300. His_Phos_1. 1 hit.
[Graphical view ]
SMARTi SM00855. PGAM. 1 hit.
[Graphical view ]
SUPFAMi SSF53254. SSF53254. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.
  2. "The mitochondrial phosphatase PGAM5 functions at the convergence point of multiple necrotic death pathways."
    Wang Z., Jiang H., Chen S., Du F., Wang X.
    Cell 148:228-243(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPGAM5_RAT
AccessioniPrimary (citable) accession number: Q562B5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 10, 2005
Last modified: October 29, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3