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Protein

UvrABC system protein B

Gene

uvrB

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi36 – 438ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Excision nuclease

Keywords - Biological processi

DNA damage, DNA excision, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1937-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein B
Short name:
Protein UvrB
Alternative name(s):
Excinuclease ABC subunit B
Gene namesi
Name:uvrB
Ordered Locus Names:TTHA1892
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 665665UvrABC system protein BPRO_0000138440Add
BLAST

Interactioni

Subunit structurei

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex (By similarity).By similarity

Protein-protein interaction databases

STRINGi300852.TTHA1892.

Structurei

Secondary structure

1
665
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi14 – 2613Combined sources
Beta strandi30 – 367Combined sources
Helixi42 – 5312Combined sources
Beta strandi57 – 637Combined sources
Helixi64 – 7714Combined sources
Beta strandi81 – 855Combined sources
Helixi89 – 913Combined sources
Beta strandi97 – 993Combined sources
Helixi100 – 1023Combined sources
Beta strandi104 – 1063Combined sources
Helixi115 – 12915Combined sources
Beta strandi133 – 1386Combined sources
Helixi139 – 1413Combined sources
Helixi148 – 1536Combined sources
Beta strandi156 – 1583Combined sources
Turni172 – 1776Combined sources
Beta strandi182 – 1843Combined sources
Beta strandi193 – 1953Combined sources
Beta strandi197 – 1993Combined sources
Beta strandi208 – 2103Combined sources
Beta strandi237 – 2393Combined sources
Helixi251 – 27121Combined sources
Helixi275 – 29521Combined sources
Helixi301 – 3044Combined sources
Helixi305 – 3095Combined sources
Helixi320 – 3234Combined sources
Beta strandi329 – 3335Combined sources
Helixi335 – 35824Combined sources
Helixi364 – 3685Combined sources
Helixi374 – 3796Combined sources
Beta strandi382 – 3909Combined sources
Helixi393 – 3986Combined sources
Beta strandi400 – 4056Combined sources
Beta strandi416 – 4205Combined sources
Helixi425 – 43814Combined sources
Beta strandi442 – 4465Combined sources
Helixi450 – 46213Combined sources
Beta strandi467 – 4704Combined sources
Helixi476 – 48712Combined sources
Beta strandi492 – 4976Combined sources
Beta strandi509 – 5146Combined sources
Turni515 – 5184Combined sources
Helixi522 – 5243Combined sources
Helixi526 – 5338Combined sources
Helixi534 – 5363Combined sources
Beta strandi543 – 5475Combined sources
Helixi553 – 57624Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1C4OX-ray1.50A2-665[»]
1D2MX-ray1.90A1-665[»]
ProteinModelPortaliQ56243.
SMRiQ56243. Positions 2-583.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ56243.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 408386Helicase ATP-bindingAdd
BLAST
Domaini425 – 591167Helicase C-terminalAdd
BLAST
Domaini611 – 64636UVRAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi89 – 11224Beta-hairpinAdd
BLAST

Domaini

The beta-hairpin motif is involved in DNA binding.By similarity

Sequence similaritiesi

Belongs to the UvrB family.Curated
Contains 1 helicase ATP-binding domain.Curated
Contains 1 helicase C-terminal domain.Curated
Contains 1 UVR domain.Curated

Phylogenomic databases

eggNOGiENOG4105CCW. Bacteria.
COG0556. LUCA.
HOGENOMiHOG000073580.
KOiK03702.
OMAiQEYVDRM.
PhylomeDBiQ56243.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB. 1 hit.
InterProiIPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q56243-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFRYRGPSP KGDQPKAIAG LVEALRDGER FVTLLGATGT GKTVTMAKVI
60 70 80 90 100
EALGRPALVL APNKILAAQL AAEFRELFPE NAVEYFISYY DYYQPEAYVP
110 120 130 140 150
GKDLYIEKDA SINPEIERLR HSTTRSLLTR RDVIVVASVS AIYGLGDPRE
160 170 180 190 200
YRARNLVVER GKPYPREVLL ERLLELGYQR NDIDLSPGRF RAKGEVLEIF
210 220 230 240 250
PAYETEPIRV ELFGDEVERI SQVHPVTGER LRELPGFVLF PATHYLSPEG
260 270 280 290 300
LEEILKEIEK ELWERVRYFE ERGEVLYAQR LKERTLYDLE MLRVMGTCPG
310 320 330 340 350
VENYARYFTG KAPGEPPYTL LDYFPEDFLV FLDESHVTVP QLQGMYRGDY
360 370 380 390 400
ARKKTLVDYG FRLPSALDNR PLRFEEFLER VSQVVFVSAT PGPFELAHSG
410 420 430 440 450
RVVEQIIRPT GLLDPLVRVK PTENQILDLM EGIRERAARG ERTLVTVLTV
460 470 480 490 500
RMAEELTSFL VEHGIRARYL HHELDAFERQ ALIRDLRLGH YDCLVGINLL
510 520 530 540 550
REGLDIPEVS LVAILDADKE GFLRSERSLI QTIGRAARNA RGEVWLYADR
560 570 580 590 600
VSEAMQRAIE ETNRRRALQE AYNLEHGITP ETVRKEVRAV IRPEGYEEAP
610 620 630 640 650
LEADLSGEDL RERIAELELA MWQAAEALDF ERAARLRDEI RALEARLQGV
660
RAPEPVPGGR KRKRR
Length:665
Mass (Da):76,160
Last modified:March 29, 2005 - v2
Checksum:i68502BFCB6F28BEF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti478 – 4781E → K in BAA08653 (PubMed:8621636).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49912 Genomic DNA. Translation: BAA08653.1.
AP008226 Genomic DNA. Translation: BAD71715.1.
RefSeqiWP_011228991.1. NC_006461.1.
YP_145158.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71715; BAD71715; BAD71715.
GeneIDi3168014.
KEGGittj:TTHA1892.
PATRICi23958745. VBITheThe93045_1859.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49912 Genomic DNA. Translation: BAA08653.1.
AP008226 Genomic DNA. Translation: BAD71715.1.
RefSeqiWP_011228991.1. NC_006461.1.
YP_145158.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1C4OX-ray1.50A2-665[»]
1D2MX-ray1.90A1-665[»]
ProteinModelPortaliQ56243.
SMRiQ56243. Positions 2-583.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1892.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71715; BAD71715; BAD71715.
GeneIDi3168014.
KEGGittj:TTHA1892.
PATRICi23958745. VBITheThe93045_1859.

Phylogenomic databases

eggNOGiENOG4105CCW. Bacteria.
COG0556. LUCA.
HOGENOMiHOG000073580.
KOiK03702.
OMAiQEYVDRM.
PhylomeDBiQ56243.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1937-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ56243.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB. 1 hit.
InterProiIPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUVRB_THET8
AccessioniPrimary (citable) accession number: Q56243
Secondary accession number(s): Q5SH38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 29, 2005
Last modified: September 7, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The ATPase activity is stimulated by single-stranded DNA. UvrB is stable up to 80 Celsius degrees.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.