Q56243 (UVRB_THET8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UvrABC system protein B
Short name=Protein UvrB

Alternative name(s):
Excinuclease ABC subunit B

Gene names

Name:	uvrB
Ordered Locus Names:	TTHA1892

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	665 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage By similarity. HAMAP MF_00204
Subunit structure	Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex By similarity.
Subcellular location	Cytoplasm HAMAP MF_00204.
Domain	The beta-hairpin motif is involved in DNA binding By similarity. HAMAP MF_00204
Miscellaneous	The ATPase activity is stimulated by single-stranded DNA. UvrB is stable up to 80 Celsius degrees. HAMAP MF_00204
Sequence similarities	Belongs to the UvrB family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 UVR domain.

Ontologies

Keywords
Biological process	DNA damage DNA excision DNA repair SOS response
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Excision nuclease
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	SOS response Inferred from electronic annotation. Source: UniProtKB-KW nucleotide-excision repair Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro excinuclease ABC activity Inferred from electronic annotation. Source: InterPro helicase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 665

665

UvrABC system protein B HAMAP MF_00204

PRO_0000138440

Regions

Domain

23 – 408

386

Helicase ATP-binding

Domain

425 – 591

167

Helicase C-terminal

Domain

611 – 646

36

UVR

Nucleotide binding

36 – 43

8

ATP Potential

Motif

89 – 112

24

Beta-hairpin HAMAP MF_00204

Experimental info

Sequence conflict

478

1

E → K in BAA08653. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

665

Helix Strand Turn

Details...

Helix

14 – 26

13

Beta strand

30 – 36

7

Helix

42 – 53

12

Beta strand

57 – 63

7

Helix

64 – 77

14

Beta strand

81 – 85

5

Helix

89 – 91

3

Beta strand

97 – 99

3

Helix

100 – 102

3

Beta strand

104 – 106

3

Helix

115 – 129

15

Beta strand

133 – 138

6

Helix

139 – 141

3

Helix

148 – 153

6

Helix

251 – 271

21

Helix

275 – 295

21

Helix

301 – 304

4

Helix

305 – 309

5

Helix

320 – 323

4

Beta strand

329 – 333

5

Helix

335 – 358

24

Helix

364 – 368

5

Helix

374 – 379

6

Beta strand

382 – 390

9

Helix

393 – 398

6

Beta strand

400 – 405

6

Beta strand

416 – 420

5

Helix

425 – 438

14

Beta strand

442 – 446

5

Helix

450 – 462

13

Beta strand

467 – 470

4

Helix

476 – 487

12

Beta strand

492 – 497

6

Beta strand

509 – 514

6

Turn

515 – 518

4

Helix

522 – 524

3

Helix

526 – 533

8

Helix

534 – 536

3

Beta strand

543 – 547

5

Helix

553 – 576

24

Sequences

	Sequence		Length	Mass (Da)
	Q56243 [UniParc]. Last modified March 29, 2005. Version 2. Checksum: 68502BFCB6F28BEF	FASTA	665	76,160
	10 20 30 40 50 60 MTFRYRGPSP KGDQPKAIAG LVEALRDGER FVTLLGATGT GKTVTMAKVI EALGRPALVL 70 80 90 100 110 120 APNKILAAQL AAEFRELFPE NAVEYFISYY DYYQPEAYVP GKDLYIEKDA SINPEIERLR 130 140 150 160 170 180 HSTTRSLLTR RDVIVVASVS AIYGLGDPRE YRARNLVVER GKPYPREVLL ERLLELGYQR 190 200 210 220 230 240 NDIDLSPGRF RAKGEVLEIF PAYETEPIRV ELFGDEVERI SQVHPVTGER LRELPGFVLF 250 260 270 280 290 300 PATHYLSPEG LEEILKEIEK ELWERVRYFE ERGEVLYAQR LKERTLYDLE MLRVMGTCPG 310 320 330 340 350 360 VENYARYFTG KAPGEPPYTL LDYFPEDFLV FLDESHVTVP QLQGMYRGDY ARKKTLVDYG 370 380 390 400 410 420 FRLPSALDNR PLRFEEFLER VSQVVFVSAT PGPFELAHSG RVVEQIIRPT GLLDPLVRVK 430 440 450 460 470 480 PTENQILDLM EGIRERAARG ERTLVTVLTV RMAEELTSFL VEHGIRARYL HHELDAFERQ 490 500 510 520 530 540 ALIRDLRLGH YDCLVGINLL REGLDIPEVS LVAILDADKE GFLRSERSLI QTIGRAARNA 550 560 570 580 590 600 RGEVWLYADR VSEAMQRAIE ETNRRRALQE AYNLEHGITP ETVRKEVRAV IRPEGYEEAP 610 620 630 640 650 660 LEADLSGEDL RERIAELELA MWQAAEALDF ERAARLRDEI RALEARLQGV RAPEPVPGGR KRKRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ATPase activity of UvrB protein form Thermus thermophilus HB8 and its interaction with DNA." Kato R., Yamamoto N., Kito K., Kuramitsu S. J. Biol. Chem. 271:9612-9618(1996) [PubMed: 8621636] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 3-12, ATPASE ACTIVITY.
[2]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB8 / ATCC 27634 / DSM 579.
[3]	"Crystal structure of Thermus thermophilus HB8 UvrB protein, a key enzyme of nucleotide excision repair." Nakagawa N., Sugahara M., Masui R., Kato R., Fukuyama K., Kuramitsu S. J. Biochem. 126:986-990(1999) [PubMed: 10578047] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
[4]	"Crystal structure of the DNA nucleotide excision repair enzyme UvrB from Thermus thermophilus." Machius M., Henry L., Palnitkar M., Deisenhofer J. Proc. Natl. Acad. Sci. U.S.A. 96:11717-11722(1999) [PubMed: 10518516] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.83 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D49912 Genomic DNA. Translation: BAA08653.1.
AP008226 Genomic DNA. Translation: BAD71715.1.

RefSeq

YP_145158.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1C4O	X-ray	1.50	A	2-665	[»]
1D2M	X-ray	1.90	A	1-665	[»]

ProteinModelPortal

Q56243.

SMR

Q56243. Positions 2-583.

ModBase

Search...

Protein-protein interaction databases

STRING

Q56243.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

3168014.

GenomeReviews

Gene locus TTHA1892 in contig AP008226_GR.

KEGG

ttj:TTHA1892.

NMPDR

fig|300852.3.peg.1503.

PATRIC

23958745. VBITheThe93045_1859.

Phylogenomic databases

eggNOG

COG0556.

HOGENOM

HBG703949.

OMA

AAQLCNE.

PhylomeDB

Q56243.

ProtClustDB

PRK05298.

Enzyme and pathway databases

BioCyc

TTHE300852:TTHA1892-MONOMER.

Family and domain databases

HAMAP

MF_00204. UvrB.
[Tree]

InterPro

IPR014001. DEAD-like_helicase.
IPR006935. Helicase/UvrB_dom.
IPR001650. Helicase_C.
IPR004807. UvrABC_suB.
IPR001943. UvrB/C.
IPR009055. UvrB_UvrC-bd_C.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]

KO

K03702.

PANTHER

PTHR24029. PTHR24029. 1 hit.

Pfam

PF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]

SMART

SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]

SUPFAM

SSF46600. UvrB_C. 1 hit.

TIGRFAMs

TIGR00631. Uvrb. 1 hit.

PROSITE

PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

UVRB_THET8

Accession

Primary (citable) accession number: Q56243
Secondary accession number(s): Q5SH38

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	March 29, 2005
Last modified:	January 25, 2012
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program