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Protein

UvrABC system protein B

Gene

uvrB

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi36 – 43ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Excision nuclease

Keywords - Biological processi

DNA damage, DNA excision, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein B
Short name:
Protein UvrB
Alternative name(s):
Excinuclease ABC subunit B
Gene namesi
Name:uvrB
Ordered Locus Names:TTHA1892
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001384401 – 665UvrABC system protein BAdd BLAST665

Interactioni

Subunit structurei

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex (By similarity).By similarity

Protein-protein interaction databases

STRINGi300852.TTHA1892.

Structurei

Secondary structure

1665
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 26Combined sources13
Beta strandi30 – 36Combined sources7
Helixi42 – 53Combined sources12
Beta strandi57 – 63Combined sources7
Helixi64 – 77Combined sources14
Beta strandi81 – 85Combined sources5
Helixi89 – 91Combined sources3
Beta strandi97 – 99Combined sources3
Helixi100 – 102Combined sources3
Beta strandi104 – 106Combined sources3
Helixi115 – 129Combined sources15
Beta strandi133 – 138Combined sources6
Helixi139 – 141Combined sources3
Helixi148 – 153Combined sources6
Beta strandi156 – 158Combined sources3
Turni172 – 177Combined sources6
Beta strandi182 – 184Combined sources3
Beta strandi193 – 195Combined sources3
Beta strandi197 – 199Combined sources3
Beta strandi208 – 210Combined sources3
Beta strandi237 – 239Combined sources3
Helixi251 – 271Combined sources21
Helixi275 – 295Combined sources21
Helixi301 – 304Combined sources4
Helixi305 – 309Combined sources5
Helixi320 – 323Combined sources4
Beta strandi329 – 333Combined sources5
Helixi335 – 358Combined sources24
Helixi364 – 368Combined sources5
Helixi374 – 379Combined sources6
Beta strandi382 – 390Combined sources9
Helixi393 – 398Combined sources6
Beta strandi400 – 405Combined sources6
Beta strandi416 – 420Combined sources5
Helixi425 – 438Combined sources14
Beta strandi442 – 446Combined sources5
Helixi450 – 462Combined sources13
Beta strandi467 – 470Combined sources4
Helixi476 – 487Combined sources12
Beta strandi492 – 497Combined sources6
Beta strandi509 – 514Combined sources6
Turni515 – 518Combined sources4
Helixi522 – 524Combined sources3
Helixi526 – 533Combined sources8
Helixi534 – 536Combined sources3
Beta strandi543 – 547Combined sources5
Helixi553 – 576Combined sources24

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C4OX-ray1.50A2-665[»]
1D2MX-ray1.90A1-665[»]
ProteinModelPortaliQ56243.
SMRiQ56243.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ56243.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 408Helicase ATP-bindingAdd BLAST386
Domaini425 – 591Helicase C-terminalAdd BLAST167
Domaini611 – 646UVRAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi89 – 112Beta-hairpinAdd BLAST24

Domaini

The beta-hairpin motif is involved in DNA binding.By similarity

Sequence similaritiesi

Belongs to the UvrB family.Curated
Contains 1 helicase ATP-binding domain.Curated
Contains 1 helicase C-terminal domain.Curated
Contains 1 UVR domain.Curated

Phylogenomic databases

eggNOGiENOG4105CCW. Bacteria.
COG0556. LUCA.
HOGENOMiHOG000073580.
KOiK03702.
OMAiQEYVDRM.
PhylomeDBiQ56243.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB. 1 hit.
InterProiIPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q56243-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFRYRGPSP KGDQPKAIAG LVEALRDGER FVTLLGATGT GKTVTMAKVI
60 70 80 90 100
EALGRPALVL APNKILAAQL AAEFRELFPE NAVEYFISYY DYYQPEAYVP
110 120 130 140 150
GKDLYIEKDA SINPEIERLR HSTTRSLLTR RDVIVVASVS AIYGLGDPRE
160 170 180 190 200
YRARNLVVER GKPYPREVLL ERLLELGYQR NDIDLSPGRF RAKGEVLEIF
210 220 230 240 250
PAYETEPIRV ELFGDEVERI SQVHPVTGER LRELPGFVLF PATHYLSPEG
260 270 280 290 300
LEEILKEIEK ELWERVRYFE ERGEVLYAQR LKERTLYDLE MLRVMGTCPG
310 320 330 340 350
VENYARYFTG KAPGEPPYTL LDYFPEDFLV FLDESHVTVP QLQGMYRGDY
360 370 380 390 400
ARKKTLVDYG FRLPSALDNR PLRFEEFLER VSQVVFVSAT PGPFELAHSG
410 420 430 440 450
RVVEQIIRPT GLLDPLVRVK PTENQILDLM EGIRERAARG ERTLVTVLTV
460 470 480 490 500
RMAEELTSFL VEHGIRARYL HHELDAFERQ ALIRDLRLGH YDCLVGINLL
510 520 530 540 550
REGLDIPEVS LVAILDADKE GFLRSERSLI QTIGRAARNA RGEVWLYADR
560 570 580 590 600
VSEAMQRAIE ETNRRRALQE AYNLEHGITP ETVRKEVRAV IRPEGYEEAP
610 620 630 640 650
LEADLSGEDL RERIAELELA MWQAAEALDF ERAARLRDEI RALEARLQGV
660
RAPEPVPGGR KRKRR
Length:665
Mass (Da):76,160
Last modified:March 29, 2005 - v2
Checksum:i68502BFCB6F28BEF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti478E → K in BAA08653 (PubMed:8621636).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49912 Genomic DNA. Translation: BAA08653.1.
AP008226 Genomic DNA. Translation: BAD71715.1.
RefSeqiWP_011228991.1. NC_006461.1.
YP_145158.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71715; BAD71715; BAD71715.
GeneIDi3168014.
KEGGittj:TTHA1892.
PATRICi23958745. VBITheThe93045_1859.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49912 Genomic DNA. Translation: BAA08653.1.
AP008226 Genomic DNA. Translation: BAD71715.1.
RefSeqiWP_011228991.1. NC_006461.1.
YP_145158.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C4OX-ray1.50A2-665[»]
1D2MX-ray1.90A1-665[»]
ProteinModelPortaliQ56243.
SMRiQ56243.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1892.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71715; BAD71715; BAD71715.
GeneIDi3168014.
KEGGittj:TTHA1892.
PATRICi23958745. VBITheThe93045_1859.

Phylogenomic databases

eggNOGiENOG4105CCW. Bacteria.
COG0556. LUCA.
HOGENOMiHOG000073580.
KOiK03702.
OMAiQEYVDRM.
PhylomeDBiQ56243.

Miscellaneous databases

EvolutionaryTraceiQ56243.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB. 1 hit.
InterProiIPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUVRB_THET8
AccessioniPrimary (citable) accession number: Q56243
Secondary accession number(s): Q5SH38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 29, 2005
Last modified: November 2, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The ATPase activity is stimulated by single-stranded DNA. UvrB is stable up to 80 Celsius degrees.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.