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Protein

Chaperone protein DnaJ 2

Gene

dnaJ2

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Does not influence ATP binding or hydrolysis nor ADP release. Exerts influence on the interaction of DnaK with substrates; in the presence of DafA, DnaJ inhibits substrate binding, and substrate already bound to DnaK is displaced by DnaJ and DafA.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

DNA replication, Stress response

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1526-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJ 2
Gene namesi
Name:dnaJ2
Ordered Locus Names:TTHA1489
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 280280Chaperone protein DnaJ 2PRO_0000070921Add
BLAST

Interactioni

Subunit structurei

Forms a heterononamer with DnaJ and DafA in the resting state. Three copies of each protein are present in the complex.

Protein-protein interaction databases

STRINGi300852.TTHA1489.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4J7ZOther1.64A/B/C/D/E/F2-114[»]
4J80Other2.90A/B/C/D1-280[»]
ProteinModelPortaliQ56237.
SMRiQ56237. Positions 5-70.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 7065JPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi75 – 806Poly-Pro
Compositional biasi81 – 11030Gly-richAdd
BLAST

Sequence similaritiesi

Belongs to the DnaJ family.Curated
Contains 1 J domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105DVC. Bacteria.
COG2214. LUCA.
HOGENOMiHOG000226716.
KOiK05516.
OMAiLEVRITI.
OrthoDBiEOG6BPDKP.
PhylomeDBiQ56237.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 2 hits.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q56237-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAKKDYYAI LGVPRNATQE EIKRAYKRLA RQYHPDVNKS PEAEEKFKEI
60 70 80 90 100
NEAYAVLSDP EKRRIYDTYG TTEAPPPPPP GGYDFSGFDV EDFSEFFQEL
110 120 130 140 150
FGPGLFGGFG RRSRKGRDLR AELPLTLEEA FHGGERVVEV AGRRVSVRIP
160 170 180 190 200
PGVREGSVIR VPGMGGQGNP PGDLLLVVRL LPHPVFRLEG QDLYATLDVP
210 220 230 240 250
APIAVVGGKV RAMTLEGPVE VAVPPRTQAG RKLRLKGKGF PGPAGRGDLY
260 270 280
LEVRITIPER LTPEEEALWK KLAEAYYARA
Length:280
Mass (Da):30,978
Last modified:July 15, 1998 - v2
Checksum:iAE32D959359388D0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti129 – 1291E → K in AAB04678 (PubMed:9349721).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L57504 Genomic DNA. Translation: AAB04678.1.
Y07826 Genomic DNA. Translation: CAA69161.1.
D84222 Genomic DNA. Translation: BAA12282.1.
AB012390 Genomic DNA. Translation: BAA81743.1.
AB032368 Genomic DNA. Translation: BAA96087.1.
AP008226 Genomic DNA. Translation: BAD71312.1.
RefSeqiWP_011228713.1. NC_006461.1.
YP_144755.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71312; BAD71312; BAD71312.
GeneIDi3170135.
KEGGittj:TTHA1489.
PATRICi23957939. VBITheThe93045_1464.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L57504 Genomic DNA. Translation: AAB04678.1.
Y07826 Genomic DNA. Translation: CAA69161.1.
D84222 Genomic DNA. Translation: BAA12282.1.
AB012390 Genomic DNA. Translation: BAA81743.1.
AB032368 Genomic DNA. Translation: BAA96087.1.
AP008226 Genomic DNA. Translation: BAD71312.1.
RefSeqiWP_011228713.1. NC_006461.1.
YP_144755.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4J7ZOther1.64A/B/C/D/E/F2-114[»]
4J80Other2.90A/B/C/D1-280[»]
ProteinModelPortaliQ56237.
SMRiQ56237. Positions 5-70.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1489.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71312; BAD71312; BAD71312.
GeneIDi3170135.
KEGGittj:TTHA1489.
PATRICi23957939. VBITheThe93045_1464.

Phylogenomic databases

eggNOGiENOG4105DVC. Bacteria.
COG2214. LUCA.
HOGENOMiHOG000226716.
KOiK05516.
OMAiLEVRITI.
OrthoDBiEOG6BPDKP.
PhylomeDBiQ56237.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1526-MONOMER.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 2 hits.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing, and expression of dnaK-operon proteins from the thermophilic bacterium Thermus thermophilus."
    Osipiuk J., Joachimiak A.
    Biochim. Biophys. Acta 1353:253-265(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Seidel R.
    Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "K+ is an indispensable cofactor for GrpE stimulation of ATPase activity of DnaK/DnaJ complex from Thermus thermophilus."
    Motohashi K., Yohda M., Odaka M., Yoshida M.
    FEBS Lett. 412:633-636(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.
  5. "The functional cycle and regulation of the Thermus thermophilus DnaK chaperone system."
    Klostermeier D., Seidel R., Reinstein J.
    J. Mol. Biol. 287:511-525(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiDNAJ2_THET8
AccessioniPrimary (citable) accession number: Q56237
Secondary accession number(s): P77642, Q5SI85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 15, 1998
Last modified: November 11, 2015
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.